Optimization of the purification methods for recovery of recombinant growth hormone from Paralichthys olivaceus

This study aimed to optimize the purification of recombinant growth hormone from Paralichthys olivaceus. Recombinant flounder growth hormone (r-fGH) was expressed by Escherichia coli in form of inclusion body or as soluble protein under different inducing conditions. The inclusion body was renatured using two recovery methods, i.e., dilution and dialysis. Thereafter, the refolded protein was purified by Glutathione Sepharase 4B affinity chromatography and r-fGH was obtained by cleavage of thrombin. For soluble products, r-fGH was directly purified from the lysates by Glutathione Sepharase 4B affinity chromatography. ELISA-receptor assay demonstrated that despite its low receptor binding activity, the r-fGH purified from refolded inclusion body had a higher yield (2.605 mgL-1) than that from soluble protein (1.964 mgL-1). Of the tested recovery methods, addition of renaturing buffer (pH 8.5) into denatured inclusion body yielded the best recovery rate (17.9%). This work provided an optimized purification method for high recovery of r-fGH, thus contributing to the application of r-fGH to aquaculture.     

Anti-proliferative activity of phlorotannin extracts from brown algae Laminaria japonica Aresch

In this study,we evaluated the anti-proliferative activity of phlorotannins derived from brown algae Laminaria japonica Aresch extracts on the human hepatocellular carcinoma cell (BEL-7402) and on murine leukemic cells (P388) by MTT assay.Cells were incubated with 100 μg/mL of the phlorotannin extract (PE) for 48 h.The inhibitory rate of PE on BEL-7402 and P388 cells was 30.20±1.16% and 43.44±1.86%,respectively,and the half-inhibitory concentration of PE (IC50) on P388 and BEL-7402 cells was 120 μg/mL and >...     

Effects of Pseudoalteromonas sp. BC228 on digestive enzyme activity and immune response of juvenile sea cucumber (Apostichopus japonicus)

A marine bacterium, Pseudoalteromonas sp. BC228 was supplemented to feed in a feeding experiment aiming to determine its ability of enhancing the digestive enzyme activity and immune response of juvenile Apostichopus japonicus. Sea cucumber individuals were fed with the diets containing 0(control), 105, 107 and 109 CFU g-1 diet of BC228 for 45 days. Results showed that intestinal trypsin and lipase activities were significantly enhanced by 107 and 109 CFU g-1 diet of BC228 in comparison with control(P 0.01). The phagocytic activity in the coelomocytes of sea cucumber fed the diet supplemented with 107 CFU g-1 diet of BC228 was significantly higher than that of those fed control diet(P 0.05). In addition, 105 and 107 CFU g-1 diet of BC228 significantly enhanced lysozyme and phenoloxidase activities in the coelomic fluid of sea cucumber, respectively, in comparison with other diets(P 0.01). Sea cucumbers, 10 each diet, were challenged with Vibrio splendidus NB13 after 45 days of feeding. It was found that the cumulative incidence and mortality of sea cucumber fed with BC228 containing diets were lower than those of animals fed control diet. Our findings evidenced that BC228 supplemented in diets improved the digestive enzyme activity of juvenile sea cucumber, stimulated its immune response and enhanced its resistance to the infection of V. splendidus.     

BDS卫星差分码偏差稳定性分析及其短期预报

利用MGEX(multi-GNSS experiment)发布的BDS卫星差分码偏差(differential code bias, DCB)产品，比较分析不同太阳活动水平下BDS卫星DCB产品的稳定性变化特性，并采用差分自回归移动平均(auto-regressive integrated moving average, ARIMA)时间序列预测模型，实现不同太阳活动水平下BDS卫星DCB的短期预报。结果表明，在太阳活动高年，BDS卫星DCB日解值稳定度、月稳定性均明显低于太阳活动低年，且不同卫星星座类型的BDS卫星DCB稳定性也存在差异；ARIMA时间序列预报结果与MGEX发布值符合程度较好，优于多项式拟合法预测结果。     

2016年门源Ms6.4地震前祁连山周边断层活动分析

基于青藏高原东北缘地区最新的跨断层资料,利用时序曲线分析、断层速率合成分析和垂直形变累积率分析等多种方法进行计算,从不同角度和范围提取2016年门源Ms6.4地震震前异常特征,揭示断层活动。结果显示：1）震中距150 km以内多个场地出现了典型的以加速张性变化为主的短期异常,150~300 km内的异常以趋势变化为主;2）祁连山-海原断裂带上异常场地的空间分布表现出由祁连山断裂带中部集中向东迁移至中东部集中的特征,海原断裂上异常有所增多,此类现象与2003年民乐6.1级地震前的现象极其相似;3）祁连山断裂自2014年以来合成速率偏离正常动态值较远,容易积累应变。垂直形变趋势累积率的计算结果显示,地震震中位于形变积累的高值区。     

本溪地区太古宙岩石中黑云母击象的发现及其地质意义

作者在辽宁省本溪地区大范围内发现黑云母击象,这在该区尚属首次。黑云母击象以及存在的地质环境表明,在地质演变史上,本溪地区曾存在至少一期影响普遍的快速构造变形—岩浆活动事件,它很可能代表一次古断裂地震活动。     

南秦岭古生代盆地演化中幕式流体过程及成岩成矿效应

从南秦岭古生代盆地构造-沉积演化出发,通过分析古生界不同层位流体成岩成矿事实与盆地发展演化之间的关系,探讨了南秦岭早古生代—泥盆纪演化时期多幕流体过程和成岩成矿效应。幕式流体成岩成矿最早可追溯到早寒武世,区域上从东到西形成了重要的碳硅质岩中重晶石/毒重石矿床及金、铂族元素矿化;早、中志留世继续发展,形成了钠长石岩、重晶石岩、铁碳酸盐岩、硅质岩与铅-锌、金矿床;中泥盆世为最强烈的一次热水沉积活动,其规模大、热水沉积产物多样,形成了泥盆系硅质岩-钠长石岩-重晶石岩-铁白云石岩中的铅锌(铜)矿床、铁白云石硅质岩中铅锌(铜)矿床以及钠长石角砾-铁白云石板岩中金-铜矿床。     

文安MS5.1地震前区域形变异常研究

对2006-07-04文安MS5.1地震前2001~2004年区域GPS站速度场和应变率场使用刚性-线弹性运动模型拟合处理的结果进行研究,获得文安MS5.1地震的1~3 a期的前兆异常。结果表明,地震发生在异常区高异常体发育的边缘梯度带的转折处。     

一种签字工作流的定义方法研究

对签字的基本模型进行了总结，并提出一种有足够建模能力、便于用户理解与计算机实现的签字过程定义方法——带条件的链表描述法。     

1984年2月14日大角星色球活动的CaⅡ H,K线观测

我们用云台一米望远镜Coudé摄谱仪得到大量的大角星(αBoo)的高分辨率光谱,从中找到1984年2月14日一组随时间变化的CallH,K线光谱。经对比分析,我们认为这可能是一次大角星的色球爆发,其特征如下:在连续观测近四小时中获得5张光谱片,可看出CallH,K线轮廓的变化。它们变化的顺序是:开始出现轮廓的不对称一轮廓仍然不对称并伴随着峰值发射增强一轮廓恢复到对称状况;K_2线中K_(2V)与K_(2r)最大不对称为20％。发射极大时K_(2S2)峰值增强20％左右。K_(1r)和K_(1V)的变化也明显。特别是K_3线在K_2线峰值增强时出现吸收线反转出发射线核。