Microbial release of potassium from K-bearing minerals by thermophilic fungus Aspergillus fumigatus

A strain of thermophilic fungus Aspergillus fumigatus was cultured with K-bearing minerals to determine if microbe-mineral interactions enhance the release of mineralic potassium. Experiments were carried out in two settings, one with the mineral grains and the fungal cells in direct contact, and the other employing a membrane (pore size 0.22 μm) to separate the two. Measurements over a period of 30 days showed that, irrespective of the experimental setup, the concentration of free K in the culture was drastically higher than those in any of the control experiments where no living organism was present. Moreover, the occurrence of mineral-cell physical contact enhanced potassium release by an additional factor of 3 to 4 in comparison to the separation experiments. For contact experiments, Electron Probe Microanalysis revealed the formation of mycelium-mineral aggregates, and Atomic Force Microscopy imaging further indicated the possible ingestion of mineral particles by the fungus cells. Contrasting to what was observed and expected in control experiments, the potassium solubilization rate showed a positive dependence upon pH when fungi and minerals were mixed directly, and exhibited no correlations with solution acidity if cell-rock contact was restrained. These results appear to suggest that A. fumigatus promoted potassium release by means of at least three likely routes, one through the complexation of soluble organic ligands, another appealing to the immobile biopolymers such as the insoluble components of secretion, and the third related to the mechanical forces in association with the direct physical contact between cells and mineral particles.     

扰动重力梯度的球冠谐分析建模

从球冠谐理论出发,详细推导了球冠坐标系下扰动重力梯度的无奇异性计算公式。基于Tikhonov正则化方法,利用GOCE卫星实际观测数据解算局部重力场球冠谐模型。数值计算表明,基于扰动重力梯度的球冠谐分析建模方法能够有效地恢复局部重力场中的短波信号,与GO_CONS_GCF_2_DIR_R5模型的差异在±0.3×10~(-5) m/s~2水平。     

北京天竺地热田东坝凹陷南部地区地下热水富集的地热地质条件分析

以北京地区地热流体中温度和矿物质含量较高的东坝凹陷南部地区作为实例,在完整的地热钻孔及相关地质资料基础上,通过对研究区断裂构造、热储层、热储盖层、地温场、流体水化学特性和富水性等地热地质条件进行分析,研究造成该区地热流体中温度、矿物质含量升高的主要原因。研究表明：受太阳宫断裂北段、良乡—前门断裂和楼梓庄断裂的切割作用,东坝凹陷南部地区热储层下落近千米,形成了一个较为封闭的“黑箱子”区域。这种独特的地质结构导致东坝凹陷南部地区接受东南城区地热田侧向补给较少,长期处于较为封闭的还原环境;太阳宫断裂北段存在深部的热源通道,地层深部的高温热流顺断裂上涌在东坝凹陷南部地区的热储层富集,因此该区地热流体表现出温度高、矿物质含量高、富水性差的特征,该研究对我国今后地热资源的开发具有积极的借鉴意义。     

盐酸普鲁卡因诱导下海洋真菌Hypocrea lixii次级代谢化学成分

【目的】通过化学诱导方法从一株海洋真菌Hypocrea lixii中获得多样化的活性小分子化合物。【方法】对菌株在盐酸普鲁卡因的化学诱导作用下进行固体发酵,代谢产物采用硅胶减压柱层析、Sephadex LH-20以及pHPLC等手段分离纯化,对化合物运用NMR、MS等波谱学技术并比对文献进行结构鉴定,采用Ellman比色法及DPPH自由基清除法对化合物进行初步抗乙酰胆碱酯酶(AChE)和抗氧化活性测试。【结果】从海洋真菌Hypocrea lixii在盐酸普鲁卡因诱导下的发酵提取物中分离得到4个异黄酮类化合物,包括6'-O-crotonylgenistin(1)、Genistein(2)、Daidzein(3)和Genistin(4),以及对氨基苯甲酸甲酯(5)、对羟基苯丙酸(6)和胞嘧啶(7),其中化合物1和6为该种中首次报道,化合物5为生物转化来源首次报道,化合物2~4可能源自培养基。在100μmol/L剂量浓度下,化合物1~4对AChE的抑制以及DPPH自由基的清除活性均较弱,化合物5和6显示了相对强的AChE抑制活性,抑制率分别为40%和38%。【结论】盐酸普鲁卡因对该菌次级代谢产生了显著的影响,为深入研究该菌株化学诱导次级代谢产物提供基础。     

原地栖息真菌Talaromyces flavus作用下不同粒径利蛇纹石的风化作用研究

主要研究了原地栖息真菌Talaromyces flavus作用下不同粒径的蛇纹石矿物粉末的风化作用。蛇纹石矿物粉末被筛分为不同粒度的4组(150~300、100~150、75~100和45~75μm)后进行真菌溶蚀实验,并使用p H计、ICP-OES、FTIR、XRD、SEM对反应产物进行了分析。结果表明,真菌作用显著加快了蛇纹石矿物的溶蚀,当蛇纹石矿物粒度降低到100μm左右时,Mg的溶出率从21.56%明显增加到35.91%。该现象可能与矿物比表面积的增大以及生物物理作用的增强有关。在此基础上粒径进一步减小并未使Mg溶出率明显提高,因此该粒径可以作为真菌对蛇纹石溶解工业化应用的最优粒径。扫描电镜观察与矿物相分析表明,反应过程中真菌分化产生的菌丝与矿物紧密接触,破坏了矿物的晶体结构,导致矿物相的改变。该研究结果可为相关菌种在浸矿等工业领域上的应用提供参考依据。     

皖南新元古代冰期地层再认识

前人认为皖南地区新元古代冰期地层存在两套冰碛岩。通过对皖南蓝田新元古界剖面和皖南地区的另外3条新元古界剖面的重新观察,发现该剖面只存在一套冰碛层和一套盖帽白云岩,均未发现间冰期地层。植被覆盖和地层错断是造成前人认为本地区新元古代冰期地层存在两套冰碛层和两套盖帽白云岩的主要原因。这一套冰碛层与中国南方扬子区南华系南沱组相当。     

京津冀地区地磁场球冠谐分析

2002年在京津冀地区进行了45个测点的地磁三分量测量,对测量资料进行通化处理,通化时间为2002年5月5日16～18时（世界时）. 通化后的观测均方差分别优于1.5nT（地磁场总强度F）,0.5′(磁偏角D和磁倾角I). 将国际参考地磁场（IGRF2000）作为地磁正常场,建立了京津冀地区地磁异常场的球冠谐模型（BTHASCH）. 球冠极的空间位置坐标为39.5°N和117.0°E,球冠半角为4°. 在模型计算过程中,球冠谐函数的截断阶数分别取为1～10. 经综合比较, 最终采用的截断阶数为5. 建立了京津冀地区参考地磁场的球冠谐模型（BTHGRF）.根据模型,绘制了京津冀地区地磁异常场图(ΔX、ΔY、ΔZ、ΔF、ΔD、ΔI)和京津冀地区地磁图(X、Y、Z、F、D、I).     

Magnetic susceptibility variations of the Ediacaran cap carbonates in the Yangtze platform and their implications for paleoclimate

Magnetic susceptibility (MS) data were obtained from 11 sections of the Doushantuo (Ediacaran) cap carbonate that directly overlies the Nantuo glacial diamictite in the southeastern margin of the Yangtze platform. The MS data revealed two regionally correlatable peaks at the bottom and top of the cap carbonate, separated by an interval of low values. The lower MS peak coincides with high percentage of insoluble siliciclastic residues that are compositionally identical to the matrix of the underlying diamictite, suggesting its origin controlled mainly by detrital components during the first phase of cap carbonate deposition at the end of the glaciation. The upper MS peak is associated with high clay content and iron sulfides, and can be interpreted as either derived from enhanced greenhouse weathering that could have brought more terrigenous components into the ocean, or the result of ocean anoxia at the late stage of cap carbonate deposition that could led to formation of abundant iron sulfides. The regionally consistent MS curves from the cap carbonates provided the first geophysical record for the rapid climate change from icehouse to greenhouse conditions in the aftermath of the Neoproterozoic “snowball Earth” event.     

四川盐边红格温泉成因研究

针对红格温泉所处的地层、地质构造、水文地质条件,结合该区域地热背景分析了红格温泉的成矿条件,研究区内深度已达上地幔的昔格达深大断裂深部热源,区内发育的断裂构造及其断裂破碎带,厚约200m的昔格达组砂、泥、页岩互层的良好隔热盖层,区内丰富的大气降水、地表水与岩溶水资源,区内地热水产出的最低侵蚀基准面（沿崖羊河谷发育的清门口断裂带）,为红格温泉的形成提供了良好的物资基础。     

Experimental Study on the Interaction Mechanism of Cap—Pile Group—Soil

Static load tests on pile group with prototype size were carried out in order to study the behavior and the working properties of the cap—pile group—soil interaction in the pile group foundation. The soil resistance under the cap, the pile shaft resistance and the tip resistance were measured by installing various measuring gauges. Based on these test results, the cap—pile group—soil interaction characteristics were analyzed. The regulations of the soil reaction on the cap, the shaft resistance and the tip resistance of pile, the mechanism of load transfer have been discussed with comparison to the result of the single pile tests. The bearing capacity of pile group is greater than the sum of the bearing capacity of the single pile obtained from testing in the same site in pile group foundation in the case presented here.