双壳贝类CK1基因家族的鉴定及表达分析

酪蛋白激酶1 (CK1)是一种重要的蛋白激酶家族,其在DNA损伤应答和修复、细胞增殖和凋亡、胚胎发育和稳态等重要的生物学过程中都具有复杂多样的调控作用。为了理解CK1基因家族在双壳贝类中的特征、进化及生物学功能,实验采用比较基因组学及生物信息学的方法对双壳贝类CK1基因家族进行了鉴定分析,通过虾夷扇贝高温应激实验,研究了CK1基因家族在高温应激时的表达规律。结果显示,在虾夷扇贝、栉孔扇贝、长牡蛎与侏儒蛤中均存在CK1α,CK1αlike,CK1δ,CK1γ3,并发现CK1ε,CK1γ1,CK1γ2在双壳贝类中发生了丢失。时空表达分析发现,双壳贝类CK1基因均在胚胎发育早期集中表达,并以多细胞/囊胚期为界呈现出母源/合子特异性表达模式。CK1为关键基因的基因共表达模块显著富集在错配修复、核苷酸剪切修复等相关通路上,暗示了CK1基因在双壳贝类胚胎发育过程中参与调控DNA损伤修复过程,从而维持早期胚胎发育时的基因组稳定性。双壳贝类CK1基因在成体中呈现组织特异的表达模式,主要在鳃和雄性性腺中具有相对较高的表达量。虾夷扇贝受到高温应激后,其鳃中PyCK1α, PyCK1αlike和PyCK1δ...     

Annual Variation of Benthic Nutrient Fluxes in Shallow Coastal Waters (Gulf of Trieste, Northern Adriatic Sea)

Abstract. Benthic fluxes of dissolved N. Si and P nutrients, alkalinity, dissolved inorganic C (DIC), and O₂ from sediments in the Gulf of Trieste (northern Adriatic, Italy) were measured monthly for 16 months, using laboratory incubated flux chambers at in siru temperatures in the dark. The annual average fluxes were: 02 = -19.3 ± 8.2, DIC = 13.7 ± 9.6, NO3 = -0.04 ± 0.16, NH4 = 0.3 ± 0.4. PO₄= 4.001 ± 0.01, Si = 0.9 ± 0.1 mmol m-² d^-1, with strong temporal fluctuations. The highest effluxes of all nutrients and DIC were observed in the summer. Small effluxes of DIC and NH4 and influxes of Si and PO4 were observed in late winter. Only NH₄ (ca. 50%) and Si (ca. 70%) fluxes were significantly correlated with temperature. This correlation suggests that the rate of downward input and the quality of sedimented organic matter (autochthonous and allochthonous) were superimposed on the temperature fluctuations. High DIC, NH₄ and Si effluxes observed in May 1993 during low temperature were due to the degradation of sedimentary organic matter produced by an early spring bloom of benthic microalgae which occurred about 6 weeks earlies while the autumn phytoplankton bloom was simultaneously reflected in enhanced benthic fluxes due to higher temperature. The role of benthic biological advection in this transport across the sediment-water interface, evaluated by comparison between measured benthic and calculated diffusive fluxes from nutrient pore water concentrations, was of minor importance. This is probably due to low infaunal activity throughout the year it was localized mostly in the narrow surficial layer. The annual average diffusive fluxes of NH4 and PO4 were higher than those measured, probably due to the presence of nitrificationdenitrifi-cation processes and redox-dependent chemical reactions at the oxic sediment-water interface, respectively. Only during bottom-water hypoxia in September 1993 did strong PO₄ effluxes prevail. Calculations based on the Redfield stoichiometry of oxic decomposition of organic N to NH4 and NO3, and differences between diffusive and measured NH₄ fluxes showed that denitrifkation averaged 0.8 mmol m^-2 d^-1. Significant correlations between NH₄ and PO₄ DIC and Si, and NH4 and Si fluxes suggested their parallel regeneration and utilization at the sediment-water interface. The nutrient fluxes observed were not significantly linked to O₂ consumption, suggesting also that anaerobic oxidation processes were important at the sediment-water interface in the gulf. The N, P and Si nutriqnts released from sediment pore waters are probably utilized in benthic microalgal and bottorn-hater primary production. This indicates that pelagic and benthic communities in the central part of the Gulf of Trieste function relatively independently of each other.     

New Experimental Platform for Studies of Turbulence and Turbulent Mixing in Accelerating and Rotating Fluids at High Reynolds Numbers

We present a new experimental platform for studies of turbulence and turbulent mixing in accelerating and rotating fluids. The technology is based on the ultra-high performance optical holographic digital data storage. The state-of-the-art electro-mechanical, electronic, and laser components allow for realization of turbulent flows with high Reynolds number (>10⁷) in a relatively small form-factor, and quantification of their properties with extremely high spatio-temporal resolutions and high data acquisition rates. The technology can be applied for investigation of a large variety of hydrodynamic problems including the fundamental properties of non-Kolmogorov turbulence and turbulent mixing in accelerating, rotating and multiphase flows, magneto-hydrodynamics, and laboratory astrophysics. Unique experimental and metrological capabilities enable the studies of spatial and temporal properties of the transports of momentum, angular momentum, and energy and the identification of scalings, invariants, and statistical properties of these complex turbulent flows.     

Physical,radiative, and dynamical processes within a nighttime marine stratus cloud

Observations taken by aircraft and conventional platforms are used to investigate dynamical, physical, and radiative processes within a marine stratus cloud during the Canadian Atlantic Storms Program (CASP) II field project which took place over the east coast of Canada. Stratus which formed over the ocean on February 6, 1992 during the nighttime, is studied to analyze cloud top and base processes. The cloud was supercooled during the study period. Fluctuations and fluxes are calculated along constant flight altitude legs approximately 100 km long in space. The scales of structures larger than 5 km are removed from the analysis using a running average technique. Droplet spectra obtained by a forward scattering spectrometer probe (FSSP) were used in a 1-D radiative transfer model to calculate infrared (IR) fluxes and radiative heating rates. A heat conservation equation was used to estimate vertical air velocity (w _a ) within the cloud. The results showed that, because of a warmer ocean surface, significant moisture and heat were transferred from the ocean surface to the boundary layer. The cloud base was at about 400 m height and the top was at about 1.4 km.w _a at the cloud base was estimated about 5 cm s^–1. Strong IR cooling rate at the cloud top was calculated to be 75°C day^–1 for a 100 m thick layer. Negative skewness inw _a , suggesting narrow downdrafts, was likely due to radiative cooling at the cloud top. The entrainment velocity was found to be about 1.5 cm s^–1 at cloud top. Mean moisture and heat fluxes within the cloud were estimated to be comparable to those from the ocean surface. Vertical air velocity at the cloud top due to radiative cooling was found to be about –40 cm s^–1.     

桂北豆乍山岩体放射性地球化学特征及其干热岩资源潜力研究

对桂北豆乍山岩体钻孔样品进行了放射性生热元素含量、岩石密度和岩石热导率测试.结果 显示该岩体花岗岩U平均含量为17.49×10-6,Th平均含量为27.54×10-6,K2O平均含量为4.64％,放射性生热率平均值6.46 μW/m3,高于地壳平均值及大部分华南其他岩体的放射性生热率值;岩石密度平均在2.57 g/cm...     

临沂市温泉（井）成热机制分析及找热方向研究

临沂市地处鲁西隆起南部,地热资源丰富。该文介绍了临沂市的地层、岩浆岩、构造、地热背景、主要控热断裂及20余眼温泉(井)的情况和分布。通过分析汤头温泉、白塔地热井、汤坊崖地热井、铜井地热井、松山地热井、新村地热井、西高都地热井等主要温泉(井)的控热断裂、成热机制,认为该区地热主要分布在沂沭断裂带与NW向深大断裂交会处,地下水补给多沿NW—SE断裂,热源以深大断裂沟通深部热源,水温、水量较好地热井均处在沂沭断裂带沂水-汤头断裂以西的、控热断裂为张性断裂或的张扭性断裂凹陷区内。结合临沂市区域地质构造特点,认为蒙山断裂与鄌郚-葛沟断裂深部交会处,尼山断裂、峄城断裂与鄌郚-葛沟断裂交会处为地热勘探靶区。     

水源热泵井回灌堵塞颗粒迁移模型的建立

以地下水源热泵井回灌中的物理堵塞问题作为研究背景,建立基于质量平衡方程来模拟多孔介质中颗粒的迁移方程和颗粒的沉积造成孔隙损伤的数学模型。该模型充分考虑了颗粒流动速度修正系数、孔隙率修正系数以及颗粒在孔隙里发生捕获率3个因素;模型的建立考虑孔隙率变化的情况,在物质的迁移和堵塞的过程中,孔隙率的变化往往也是个动态的变化过程;与传统的过滤模型相比,考虑了速度折减系数和流量折减系数后新建模型更加系统、全面。该模型为解决回灌井物理堵塞的室内外试验研究提供理论依据。     

SENSITIVITY TESTS OF INTERACTION BETWEEN LAND SURFACE PHYSICAL PROCESS AND ATMOSPHERIC BOUNDARY LAYER*

In this paper,an interactive model between land surface physical process and atmosphere boundary layer is established,and is used to simulate the features of soil environmental physics,surface heat fluxes,evaporation from soil and evapotranspiration from vegetation and structures of atmosphere boundary layer over grassland underlying.The sensitivity experiments are engaged in primary physics parameters.The results show that this model can obtain reasonable simulation for diurnal variations of heat balance,soil volumetric water content,resistance of vegetation evaporation,flux of surface moisture,and profiles of turbulent exchange coefficient,turbulent momentum,potential temperature,and specific humidity.The model developed can be used to study the interaction between land surface processes and atmospheric boundary layer in city regions,and can also be used in the simulation of regional climate incorporating a mesoscale model.     

中国邻海海-气热量、水汽通量计算和分析

使用长年代、高质量海洋气象观测资料,选用适合研究海域的计算参数,按2°×2°网格计算了中国邻海（黄、渤、东、南海及140°E以西西北太平洋）辐射平衡和海-气热量、水汽通量,讨论了它们的年、月分布和时空变化,并以海面热量净收支为主要指标进行了海洋气候区划。     

中国城市群热岛效应时空演变及其影响因素分析

基于长期不透水面和MODIS地表温度数据,分析2000—2015年中国城市群扩张及热岛效应时空演变,进而综合采用冗余分析(RDA)、线性回归分析和变异分配分析(VPA)等方法,揭示城市群城市热岛效应的驱动机制。结果表明：城市群内建成区面积快速扩张,不透水面比例从2000年2.08%增长到2015年5.33%,且主要集中于珠江三角洲等沿海城市群;2000—2015年,夏季热岛分布较广,且白天强度高于夜晚。东部以及大部分北部城市群如哈长城市群等,降温强度较大,但其夜晚热岛效应在不同程度增强。冬季夜晚比白天热岛分布广、强度高,北方、西北、东部等地区夜晚热岛效应也在增强;自然环境因素显著影响城市群热岛强度,降水对夏季夜晚热岛强度起显著负贡献(22%),纬度越高,冬季夜晚热岛强度也越高。人为因素显著影响夜晚热岛分布和城市群内热岛强度的平衡,城市植被覆盖显著减少夜晚城市群内热岛分布,灯光强度对夏季夜晚热岛强度起显著负贡献(24%),对热岛比例起显著正贡献(27%),人口密度对夏季夜晚热岛强度起显著负贡献(31%);自然环境因素对热岛强度的贡献占主导,而人为干扰因素对热岛比例的贡献占主导。