中国近海藻毒素及有毒微藻产毒原因种调查研究进展

麻痹性贝类毒素在我国近海污染问题已十分突出,基本呈现逐年加剧的趋势。20世纪90年代,南海麻痹性贝类毒素污染较重; 21世纪初,北黄海麻痹性贝类毒素污染较重;近几年,渤海和福建近海麻痹性贝类毒素污染较重;可产生麻痹性贝类毒素的微藻有亚历山大藻和裸甲藻等。采用小鼠生物法检测我国近海腹泻性贝类毒素超标率32%左右,采用液相色谱/质谱法检测,仅有3起超标的研究报道;现行小鼠生物法检测腹泻性贝类毒素假阳性问题十分突出,应尽快废除;腹泻性贝类毒素均是脂溶性的,脂溶性海洋生物毒素在我国近海常年可检出,偶有虾夷扇贝毒素和鳍藻毒素超标现象。可产生脂溶性毒素微藻有鳍藻和原甲藻及网状原角藻等。失忆性贝类毒素在我国近海常有检出,但无超标现象;产毒微藻有拟菱形藻等。西加鱼毒素在我国南海污染较重,但毒素标准物质的匮乏,限制了西加鱼毒素的调查研究;至今尚未确定产生西加鱼毒素的微藻。酶联免疫吸附法和液相色谱串联质谱法相结合已成为藻毒素快速准确检测成熟的技术,逐渐代替小鼠生物法和液相色谱法。     

基于F型mtDNA D-Loop的厚壳贻贝(Mytilus coruscus)群体遗传多样性研究

为研究东海区厚壳贻贝(Mytilus coruscus)遗传多样性,以厚壳贻贝F mtDNA D-loop为标记,对浙江省舟山嵊山岛、宁波渔山岛、温州南麂岛、福建省宁德湾和莆田南日岛五个海区的厚壳贻贝群体进行了遗传分析。结果表明,厚壳贻贝各群体的遗传多样性差异不明显,在5个群体中,宁德群体的遗传多样性相对最丰富;将5个群体作为一个整体时,呈现出较高的单倍型多样性和较低的核苷酸多样性。对厚壳贻贝5个群体间的遗传分化系数(Fst)和基因流(Nm)进行检测,结果显示群体间Fst值都很低,但Nm值都很高(Nm绝对值1),表明5个群体间存在丰富的基因交流。但宁德群体与浙江沿海的3个群体(嵊山、渔山、温州)的Fst值相对较高,且差异显著(P0.5),表明宁德群体与这3个群体间出现遗传分化。本研究旨在为海洋经济贝类资源的保护管理提供理论依据。     

南海大洋钻探及海洋地质与地球物理前沿研究新突破

南海是西太平洋地区规模最大且具有代表性的边缘海盆地之一。经过近几十年的研究积累,尤其是通过实施5个国际大洋钻探航次(1999–2018年)与国家自然科学基金委“南海深海过程演变”重大研究计划(2011–2019年),我国科学家获得了大量宝贵的第一手资料,取得了一系列创新进展与重大突破,标志着南海海洋地质与地球物理研究正走向国际前沿。重要研究成果包括:(1)新提出南海是“板缘张裂”盆地,与经典的大西洋型陆缘模式不同;(2)大洋钻探首次获取了基底玄武岩样品,结合中国在南海首次深拖地磁测量实验,精确测定了南海海盆玄武岩年龄,揭示南海海盆从东向西分段扩张;(3)大洋钻探结果发现南海陆缘岩石圈减薄之初岩浆迅速出现,未发现缓慢破裂造成的蛇纹岩出露;(4)发现南海扩张结束后仍存在大量岩浆活动,可能受控于多种构造与地幔因素;(5)地球化学证据与地球动力学模拟都显示南海岩浆的形成受到周边俯冲带的影响。目前我国的海洋地球科学正在进入崭新的发展阶段,有望以南海为基点,开始拓展到周边大洋,通过主导大型研究计划以及建设我国大洋钻探平台,以提升我国在南海、西太平洋与印度洋海洋地质科学研究的实质性影响力与引领地位。     

卡里马塔海峡水体交换的季节变化

Four trawl-resistant bottom mounts, with acoustic Doppler current profilers(ADCPs) embedded, were deployed in the Karimata Strait from November 2008 to June 2015 as part of the South China Sea-Indonesian Seas Transport/Exchange and Impact on Seasonal Fish Migration(SITE) Program, to estimate the volume and property transport between the South China Sea and Indonesian seas via the strait. The observed current data reveal that the volume transport through the Karimata Strait exhibits significant seasonal variation. The winteraveraged(from December to February) transport is –1.99 Sv(1 Sv=1×10~6 m~3/s), while in the boreal summer(from June to August), the average transport is 0.69 Sv. Moreover, the average transport from January 2009 to December2014 is –0.74 Sv(the positive/negative value indicates northward/southward transport). May and September are the transition period. In May, the currents in the Karimata Strait turn northward, consistent with the local monsoon. In September, the southeasterly trade wind is still present over the strait, driving surface water northward, whereas the bottom flow reverses direction, possibly because of the pressure gradient across the strait from north to south.     

2015年台风“彩虹”过境后下山冷急流引起的南海北部海域异常海面降温

This study deals with a unusual cooling event after Typhoon Mujigea passed over the northern South China Sea(SCS) in October 2015. We analyze the satellite sea surface temperature(SST) time series from October 3 to 18,2015 and find that the cooling process in the coastal ocean had two different stages. The first stage occurred immediately after typhoon passage on October 3, and reached a maximum SST drop of –2℃ on October 7 as the usual cold wake after typhoon. The second stage or the unusual extended cooling event occurred after 7d of the typhoon passage, and lasted for 5d from October 10 to 15. The maximum SST cooling was –4℃ and occurred after 12d of typhoon passage. The mechanism analysis results indicate that after landing and moving northwestward to the Yunnan-Guizhou Plateau(YGP), Typhoon Mujigea(2015) met the westerly wind front on October 5. The lowpressure and positive-vorticity disturbances to the front triggered meridional air flow and low-pressure trough,thus induced a katabatic cold jet downward from the Qinghai-Tibet Plateau(QTP) passing through the YGP to the northwestern SCS. The second cooling reached the maximum SST drop 4d later after the maximum air temperature drop of –9℃ on October 11. The simultaneous air temperature and SST observations at three coastal stations reveal that it is this katabatic cold jet intrusion to lead the unusual SST cooling event.     

南海东北部内潮的模态结构和传播

The evolution of energy, energy flux and modal structure of the internal tides(ITs) in the northeastern South China Sea is examined using the measurements at two moorings along a cross-slope section from the deep continental slope to the shallow continental shelf. The energy of both diurnal and semidiurnal ITs clearly shows a～14-day spring-neap cycle, but their phases lag that of barotropic tides, indicating that ITs are not generated on the continental slope. Observations of internal tidal energy flux suggest that they may be generated at the Luzon Strait and propagate west-northwest to the continental slope in the northwestern SCS. Because the continental slope is critical-supercritical with respect to diurnal ITs, about 4.6 kJ/m~2 of the incident energy and 8.7 kW/m of energy flux of diurnal ITs are reduced from the continental slope to the continental shelf. In contrast, the semidiurnal internal tides enter the shelf because of the sub-critical topography with respect to semidiurnal ITs.From the continental slope to the shelf, the vertical structure of diurnal ITs shows significant variation, with dominant Mode 1 on the deep slope and dominant higher modes on the shelf. On the contrary, the vertical structure of the semidiurnal ITs is stable, with dominant Mode 1.     

再探温跃层有孔虫B/Ca与温度和[CO32-]的依赖性及其在24 ka以来热带西太平洋次表层碳酸盐系统重建中的应用

The B/Ca ratio of planktonic foraminifer shells has been used as a proxy for reconstructing past ocean carbonate chemistry. However, recent studies have revealed significant uncertainties associated with this proxy, such as whether seawater temperature or [ CO_3~(2-)] is the dominant control on the partition coefficient(K_D) of planktonic foraminiferal B/Ca. To address these uncertainties and thus improve our understanding of the planktonic foraminiferal B/Ca proxy, we analysed B/Ca ratios in the tests of Neogloboquadrina dutertrei(300–355 μm) and Pulleniatina obliquiloculata(355–400 μm) in surface sediment samples from the tropical western Pacific and South China Sea. The relationship between these B/Ca ratios and bottom water calcite saturation states(Δ[ CO_3~(2-)]) is weak, thus suggesting only a small dissolution effect on the B/Ca of the two species. The correlation coefficients(R~2) between the B/Ca ratios of N. dutertrei and P. obliquiloculata and environmental parameters(e.g., temperature, salinity, phosphate, DIC and ALK) in the tropical western Pacific and South China Sea are not high enough to justify using B/Ca ratios as a palaeoenvironmental proxy in the study areas. The significant correlation between K_D values of N. dutertrei and P. obliquiloculata and carbonate system parameters(e.g.,[ CO_3~(2-)], DIC, ALK, pH and [ HCO_3~-]) in the study area reflect chemical links between the K_D denominator and these variables. Based on our surface sediment calibration, an empirical relationship between the K_D of N.dutertrei and temperature is proposed in the tropical western Pacific. We also generated a record of B/Ca ratios in N. dutertrei(300–355 μm) from Core MD06-3052 in the tropical western Pacific over the past 24 ka to evaluate the application of the revised B/Ca proxy method. Based on the reconstructed empirical relationship for B/Ca and subsurface seawater ALK, we estimated subsurface seawater carbonate system parameters in the tropical western Pacific since 24 ka. In general, the estimated subsurface seawater pH and [ CO_3~(2-)] show an increase with time, and the record of subsurface seawater pCO_2 shows a decrease with time, in the tropical western Pacific over the past 24 ka. The consistent trends in subsurface seawater pCO_2 and opal flux during deglaciation may imply that the reported increase in subsurface water pCO_2 in the study area was promoted by enhanced upwelling in the Southern Ocean.     

南海北部台风海浪谱的双峰性和成长性

本论文通过对南海北部三次台风过境期间基于浮标观测的海浪谱进行分析,发现虽然大部分成熟的台风海浪谱为单峰结构,但实际上在台风海浪的成长和衰减阶段,双峰谱占据了很大的比例。双峰谱的形成主要是由于风浪和涌浪的叠加以及不同波分量之间的非线性相互作用,我们可以通过能量密度的成长率对谱型变化进行高效的预报。此外,台风海浪的主要波向依赖于台风中心相对观测点的位置,而波向的分散情况在相距台风中心较远的区域无明显规律。本文提出了一个新的六参数波浪谱型拟合双峰谱,其拟合效果相较于前人的谱型更好。通过验证,形状参数和谱宽度之间的理论关系依然适用于单个谱峰。通过分析谱参量的变化特征,证明了谱参量不仅与台风强度和台风路径相关,还存在很强的交互相关。最后通过拟合海浪谱数据,本文得到了台风影响下海浪有效波高和有效周期之间的成长关系,这对海洋工程实际应用具有重要意义。     

南海沉积物细菌胞外蛋白酶在家族水平上的多样性

海洋产蛋白酶细菌及其分泌的胞外蛋白酶在降解有机氮以推动海洋氮循环进行方面发挥着重要作用,但目前对这二者多样性的认识非常有限。本研究自南海沉积物中筛选得到90株产蛋白酶细菌,并通过N-端氨基酸序列,分析了其胞外蛋白酶在家族水平上的多样性。16S rRNA基因序列分析的结果表明,筛选的产蛋白酶细菌均属于Gammaproteobacteria纲,且大多数属于Alteromonadales目和Vibrionales目的不同属。对其中14株菌株的14个胞外蛋白酶的N-端氨基酸序列分析表明,所有这些蛋白酶属于金属蛋白酶的M4家族或丝氨酸蛋白酶的S8家族。本研究提供了海洋沉积物产蛋白酶细菌在类群及其胞外蛋白酶在类型上的新细节,这将有助于全面了解微生物酶促降解海洋沉积物有机氮的过程和机理。     

140kaBP以来南海西南部上升流影响区沉积记录及其古海洋环境变化

对南海西南部现代上升流区沉积物柱样BIS-187-61孔有机碳、生物蛋白石、碳酸盐等各沉积组分进行了详细的分析,试图探讨末次间冰期(MIS 5)以来南海西南部夏季上升流影响区沉积过程及其所反映的海洋环境变化过程。研究结果发现,140kaBP以来该孔沉积记录期总体表现为:碳酸盐百分含量冰期低间冰期高,主要受周边陆源物质输入稀释的控制,为典型的"大西洋型碳酸盐旋回",有机碳、生物蛋白石、碳酸钙以及陆源沉积物的堆积速率均在MIS 2期、MIS 4期和MIS 5e期出现高值。其中,MIS 2期和MIS 4期生源和陆源沉积堆积速率增加可能与冰期时冬季风增强及海平面下降导致陆源营养物质输入增加对初级生产力的刺激有关,也可能与陆源物质输入增加对海洋生源颗粒物输出的压载作用增强有关。而MIS 5e期南海海平面高度与现代相仿,间冰期较强的夏季风引起的越南沿岸上升流增强是导致该时段本研究区域表层初级生产力增强,沉积物总堆积速率及各组分堆积速率都相应增加的主要原因。