Stable 85Rb micro vapour cells: fabrication based on anodic bonding and application in chip-scale atomic clocks

We describe the microfabrication of ⁸⁵Rb vapour cells using a glass-silicon anodic bonding technique and in situ chemical reaction between rubidium chloride and barium azide to produce Rb. Under controlled conditions, the pure metallic Rb drops and buffer gases were obtained in the cells with a few mm³ internal volumes during the cell sealing process. At an ambient temperature of 90 ℃ the optical absorption resonance of ⁸⁵Rb D1 transition with proper broadening and the corresponding coherent population trapping (CPT) resonance, with a signal contrast of 1.5% and linewidth of about 1.7 kHz, have been detected. The sealing quality and the stability of the cells have also been demonstrated experimentally by using the helium leaking detection and the after-9-month optoelectronics measurement which shows a similar CPT signal as its original status. In addition, the physics package of chip-scale atomic clock (CSAC) based on the cell was realized. The measured frequency stability of the physics package can reach to 2.1×10^-10 at one second when the cell was heated to 100 ℃ which proved that the cell has the quality to be used in portable and battery-operated devices.     

渤海海效应暴雪的三维热力结构特征

采用烟台多普勒天气雷达、天气图等观测资料和中尺度模式(RAMS4.4)进行数值模拟,分析了2008年12月4~6日发生的一次海效应暴雪的时空分布和三维热力结构特征。结果表明:(1)渤海海效应降雪并非传统认为的仅发生在蓬莱以东的山东半岛地区,而是广泛分布于山东半岛、莱州湾、渤海中东部和黄海西部洋面上;降雪源地有暖海面和陆地,即莱州湾、渤海中部至渤海海峡和山东半岛。(2)浅层对流是海效应降雪的重要热力特征,当强冷空气流经渤海时,暖海面通过湍流交换等作用向冷空气底层输送感热和潜热,使得低层增温增湿,产生对流层中上层干冷低层暖湿的对流性不稳定。(3)冷空气的强弱影响渤海暖海面及山东半岛地区的垂直热力结构,导致降雪强度在不同时段存在显著差异。海气温差与热通量成正比,初期冷空气弱,对流层中低层的垂直温差小,海面上空的暖湿层浅薄,不稳定能量弱,产生的降雪量小;中后期冷空气强盛,对流层中低层的垂直温差大,暖湿层较为深厚,不稳定能量增强,导致降雪强度和降雪量大。(4)与单一要素温度和湿度相比,对流层低层相当位温的水平分布对强降雪落区具有更好的指示意义,强降雪发生在高相当位温脊线附近。     

凡纳滨对虾性别分化的初步研究

通过组织学连续切片与扫描电镜观察,研究凡纳滨对虾的性别分化。结果显示:凡纳滨对虾外部形态的性别分化最早发生在仔虾后第37天,此时在扫描电镜下可观察到雄性附肢的芽基,仔虾后第45天,光镜下可观察到交接器;仔虾后第24天出现原始生殖细胞(PGCS),位于背大动脉的两侧及围心腔膜的腹面,细胞个体及细胞核明显比周围的细胞大,具有强嗜碱性,细胞核圆形或椭圆形,核内染色质清晰可见,随着生殖腺的发育,在第43天出现生殖管,第55天生殖腺可区分为精巢和卵巢。     

斜带髭鲷后肠、头肾和脾脏中肥大细胞超微结构观察

应用电镜技术研究了斜带髭鲷的后肠、头肾及脾脏中肥大细胞的超微结构。结果显示:在所研究的器官组织中均发现有肥大细胞,但以后肠和头肾分布较多;这些肥大细胞的表面具有胞质突起或表面折叠。根据其特征性胞浆颗粒所含特殊亚结构的不同,可初步认为斜带髭鲷肥大细胞可能存在Ⅰ型和Ⅱ型两种亚型。Ⅰ型肥大细胞主要分布于后肠粘膜固有层、粘膜下层和头肾的结缔组织中,其特征性胞浆颗粒具有微粒样特殊亚结构;Ⅱ型肥大细胞仅分布于头肾和脾脏的结缔组织中,其特征性胞浆颗粒则具有漩涡样、线团样、蜂巢样、细粒状、无定形和混合型等多种晶格状特殊亚结构。同时,这两种亚型肥大细胞均存在脱颗粒现象。由观察结果可知,斜带髭鲷后肠、头肾及脾脏中存在大量的肥大细胞,并且其特征性胞浆颗粒具有精细的特殊亚结构。     

Initiation of primary cell culture from amphioxus <Emphasis Type="Italic">Branchiostoma belcheri tsingtauense</Emphasis>

Amphioxus, a cephalochordate, is an important model fish for studies in evolution and comparative biology. A successful cell culture from amphioxus tissues in vitro would help understanding some basic issues. To determine the optimal culture conditions for proliferation of amphioxus cells, primary cultures were initiated from buccal cirri, tail, gill, gut and metapleural fold of amphioxus Branchiostoma belcheri tsingtauense. The media tested were L-15, F-12, M 199, MEM, DMEM, PRMI 1640 and LDF, each was supplemented with 20% fetal bovine serum. The optimal conditions include tail tissue cultured in L-15 or F-12 with supplement of 20% FBS and 1.5% NaCl at about 25°C. Supported by Doctoral Initial Fund of Ludong University (No.43304)     

Characterization of a new lectin involved in the protoplast regeneration of <Emphasis Type="Italic">Bryopsis hypnoides</Emphasis>

A group of coenocytic marine algae differs from higher plants,whose totipotency depends on an intact cell(or protoplast).Instead,this alga is able to aggregate its extruded protoplasm in sea water and generate new mature individuals.It is thought that lectins play a key role in the aggregation process.We purified a lectin associated with the aggregation of cell organelles in Bryopsis hypnoides.The lectin was ca.27 kDa with a pI between pH 5 and pH 6.The absence of carbohydrate suggested that the lectin was ...     

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青藏高原上中尺度对流系统东移传播成因

运用1998年夏季日本静止气象卫星探测反演出的红外辐射亮温资料,对青藏高原上的中尺度对流系统(MCSs)进行了自动追踪,进而对移出高原的MCSs进行了分类,在此基础上,运用空间数据挖掘中的决策树方法得到了东移出高原的MCSs与其环境物理量场之间的关系,结果表明,在400hPa上,当MCSs的中心位置位于100°E附近时,移出高原且向东南方向移动的MCSs仅与该等压面上的高度和温度有关;而向东北方向移动的MCSs仅与高度和假相当位温有关;此外,移出高原且方向向东的MCSs主要决定于等压面高度、散度、涡度和假相当位温这4个物理量值。在500hPa上,位于东经104°E以西且最终移出高原的MCSs,决定其是否移向东北方向的环境物理量因素为K指数和水汽通量散度,而影响其向东南及东方向移动的因素则较多。     

北黄海典型水域春夏季浮游植物的昼夜变化

根据黄海西北部2006年夏季3个连续站和2007年春季1个连续站垂直分层拖网的调查资料,研究了黄海西北部典型海区浮游植物的昼夜垂直变化.夏季共检出浮游植物79种,主要优势种为旋链角毛藻Chaetoceros curvisetus、梭角藻Ceratium fusus、三角角藻Ceratium tripos和具槽帕拉藻Paralia sulcata;春季检出51种,主要优势种为浮动弯角藻Eucampia zodiacus、具槽帕拉藻和尖刺伪菱形藻Pseudo-nitzschia pungens.夏季L01站受潮汐的影响各水层低潮期浮游植物细胞丰度高于高潮期,垂直分布趋势为表层细胞丰度最高,底层最低.而由于底栖硅藻的细胞再悬浮作用夏季L02、L03站和春季L02站底层细胞丰度高于表层,但各水层细胞丰度的昼夜变化相对较平缓.