蛋白质组学的研究概况及其在海洋生物学中的应用

随着DNA序列测定技术的飞速发展，人们可以在很短的时间内获得某种生物基因组的全序列。生物体的生命活动主要依赖于基因组的产物——蛋白质组，但是蛋白质组的研究相对于基因组来说却要复杂得多。蛋白质组技术是在细胞内整体蛋白质水平上进行研究，从蛋白质整体活动的角度来研究生命活动的规律。综述了蛋白质组和蛋白质组学出现的背景、起源、概念、研究技术、研究内容、面对的挑战、开展该项研究的意义、陆生动植物和微生物蛋白质组学的研究进展及海洋生物蛋白质组学的研究概况。目前，普通生物方面的蛋白质组工作开展得较多，海洋生物方面却相对滞后。种种迹象表明，开展海洋生物蛋白质组学研究，对揭示生命的奥秘、促进海洋资源的开发都有重大的推动作用。     

湍流数值模拟中封闭模式应用的局限性

通过对琼州海峡的潮流场特征进行数值模拟，指出了选择不同的特征混合长度表达式对数值模拟结果的影响，表明了基于特征混合长度理论的流封闭模式在近海湍流数值模拟中应用的局限性。     

一个湍能封闭模型对风暴潮海底底应力的应用研究

将一个三维湍能封闭模型应用于开阔海区的风暴潮，通过数值计算探讨了Ｔａｙｌｏｒ底摩擦二次率的拖曳系数随空间的分布及拖曳系数与水深、海底粗糙度、风向和风速等因素的关系。本文对底摩擦二次率的可靠性做了评价。     

Assessment of the effects of hydrocarbon contamination on the sedimentary bacterial communities and determination of the polar lipid fraction purity: Relevance of intact phospholipid analysis

This in situ study reports on the relevance of intact phospholipids as new biomarkers and highlights the limits of the classical fatty acid analysis after the hydrolysis of the polar lipid fraction. The analysis of polar lipid fractions revealed significant contributions of marine macrophytes with either non-phospholipid compounds (sulfoquinovosyl diacylglycerol) or with both non-bacterial phospholipid classes (phosphatidylcholine) and non-bacterial phospholipid molecular species (some molecular species of phosphatidylglycerol). The analysis of intact phospholipids showed also the predominance of gram-negative bacteria in sediments whereas the analysis of fatty acids alone cannot confirm such information. Lastly, as regard to the effects of petroleum hydrocarbons on the sedimentary bacterial communities, we observed a higher degree of saturation of the fatty acyl chains of the phospholipids extracted from sediment exposed to a massive and chronic crude oil contamination. Such a result was interpreted as an adaptive response resulting in higher membrane rigidity of the bacterial communities.     

烟威及石岛近海春季水团分析

本文使用1960—1980年春季的水文资料,以温度和盐度作为主要指标,根据聚类分析逼近温盐图解,结合对该海域地理环境特征的分析,对烟台、威海及石岛近海春季的水团结构演变及其和渔场、渔期的关系进行综合分析。结果表明,北黄海及青岛外海的冷水团,以及黄海暖水团的强度与位置的变动,对春季渔期的早迟以及渔场位置的变动,都有很大的影响。     

Trace gas exchange at the air/water interface: Measurements of mass accommodation coefficients

A liquid jet of 90 m diameter and variable length has been utilized to determine absorption rates and, hence, mass accommodation coefficients , of atmospheric trace gases. The compounds investigated are HCl (0.01), HNO₃ (0.01), N₂O₅ (0.005), peroxyacetyl nitrate (>0.001), and HONO (0.005). It is concluded that the absorption of these trace gases by liquid atmospheric water is not significantly retarded by interfacial mass transport. The strengths and limitations of the liquid jet technique for measuring mass accommodation coefficients are explored.     

Water–rock interaction during the process of steam stimulation exploitation of viscous crude oil in Liaohe Shuguang Oil Field, Liaoning, China

In the process of steam stimulation exploitation of viscous crude oil, the injected water, at high temperature and under high pressure, reacts intensively with the host rock. This kind of water–rock interaction in Liaohe Shuguang Oil Field was studied on the basis of analysis of water composition changes, laboratory experiments, mineral saturation indices analysis, and mass balance calculation. Compared with the injected water, the changes of the composition of discharged water are mainly the distinct decrease of pH, Na⁺, SiO₂ and Cl⁻, as well as the increase of K⁺, Ca²⁺, Mg²⁺, SO ₄ ²⁻ and HCO ₃ ⁻ . Laboratory experiments under field conditions showed: the dissolution sequence of minerals quantitatively is quartz>potassium feldspar>albite, and the main change of clay minerals is the conversion of kaolinite to analcime. Mass balance calculation indicated during the process of steam stimulation, large quantities of analcime are precipitated with the dissolution of large amounts of quartz, kaolinite, potassium feldspar, and CO₂. These results correlated very well with the experimental results. The calculated results of Liaohe Shuguang Oil Field showed that during the steam stimulation for viscous crude oil, the amounts of minerals dissolved (precipitated) are huge. To control the clogging of pore spaces of oil reservoirs, increased study of water–rock interaction is needed.     

Sources of Sediment in Lake Pepin on the Upper Mississippi River in Response to Holocene Climatic Changes

Sediments from Lake Pepin on the Mississippi River, southeastern Minnesota, are used as provenance tracers to assess variations in hydrology and sediment-transport during the middle Holocene. Three rivers contribute sediment to Lake Pepin, and each catchment is characterized by a distinctly different geologic terrain. The geochemical fingerprint for each drainage basin was determined from the elemental composition of heavy minerals in the silt-sized fraction of modern sediment samples. Down-core elemental abundances were compared with these fingerprints by use of a chemical-mass-balance model that apportions sediment to the source areas. We observed a decreased contribution from the Minnesota River during the interval ~6700–5500 ¹⁴C yr BP, which we attribute to decreased discharge of the Minnesota River, likely controlled by a combination of precipitation, snow melt, and groundwater input to the river. This hydrologic condition coincides with the mid-Holocene prairie period recorded by fossil pollen data. The occurrence of this feature in a proxy record for hydrologic variations supports the hypothesis that the mid-Holocene prairie period reflects drier conditions than before or after in midwestern North America.     

三维遥感影像数据库系统的研究与实现

介绍了以3S技术为手段，采用目前较为先进的三层结构体系，使用Dephi开发工具，结合数据库技术、遥感技术，尝试以数据库SQL Server组织、存储、管理海量的中巴资源卫星遥感影像数据和新疆1：10万地形矢量数据，应用OpenGL技术实现海量三维遥感影像数据的三维立体显示浏览，进而完成全疆三维遥感影像库建库和三维显示及漫游．     

中尺度模式中的边界层特征量分析

在中尺度数值天气预报模式MM4中，用level3及E-ε-l两种湍流闭合方法对原总体边界层参数化方案进行改进，使得中尺度模式中可以直接输出有关湍流量，并对地表通量参数化方案进行改进，进而对不同下势需如沙漠，植被上的有关湍流量和边界层特征量进行分析。研究中尺度系统中边界层结构的特征，本文主要从地表通量，湍流交换系数，湍流动能（q^2)，温度脉动方差（θ^2)及风温廓线等几个方面进行研究，结果表明，新方案能更好地反映边界层特征。