The DNA de-methylating agent 5-azacytidine does not restore CYP1A induction in PCB resistant Newark Bay killifish (Fundulus heteroclitus)

Newark Bay (NB) killifish (Fundulus heteroclitus) have been chronically exposed to environmental contaminants that activate the aryl hydrocarbon receptor (AHR) and are tolerant to toxic effects and CYP1A induction provoked by AHR ligands. Resistance to CYP1A induction could be due to an epigenetic mechanism such as DNA methylation. We measured in-ovo CYP1A catalytic activity (ethoxyresorufin-O-deethylase, EROD) in NB and reference site killifish embryos aqueously exposed to various concentrations of the de-methylating agent 5-azacytidine, 5-AC (5, 50 and 500 μ(micro)M) with or without 0.2 μ(micro)g/l of the CYP1A inducer 3,3^′,4,4^′,5 pentachlorobiphenyl (IUPAC PCB126). Neither PCB126 alone, nor PCB126 plus 5-AC, induced EROD above levels in vehicle treated Newark Bay fish. In reference site fish, the same PCB126 dose provoked a 7.4-fold EROD induction relative to controls. We conclude that Newark Bay killifish are resistant to CYP1A induction by co-planar PCBs during early embryological development and our data suggests that DNA methylation does not play a critical role in resistance to CYP1A induction in this model.     

Data assimilation for a coastal area morphodynamic model: Morecambe Bay

This paper investigates the use of data assimilation in coastal area morphodynamic modelling using Morecambe Bay as a study site. A simple model of the bay has been enhanced with a data assimilation scheme to better predict large-scale changes in bathymetry observed in the bay over a 3-year period. The 2DH decoupled morphodynamic model developed for the work is described, as is the optimal interpolation scheme used to assimilate waterline observations into the model run. Each waterline was acquired from a SAR satellite image and is essentially a contour of the bathymetry at some level within the inter-tidal zone of the bay. For model parameters calibrated against validation observations, model performance is good, even without data assimilation. However the use of data assimilation successfully compensates for a particular failing of the model, and helps to keep the model bathymetry on track. It also improves the ability of the model to predict future bathymetry. Although the benefits of data assimilation are demonstrated using waterline observations, any observations of morphology could potentially be used. These results suggest that data assimilation should be considered for use in future coastal area morphodynamic models.     

Experimental investigation on a two-part underwater towed system

An experimental set-up is developed and proved to be effective for laboratory study of an underwater towed system. The experimental technique gives a practical method for monitoring the kinematic and dynamic performance of an underwater towed system in a ship towing tank. Both the theoretical and experimental results in the investigation indicate that the hydrodynamic response of a towed vehicle to the wave induced motion of a towing ship can be significantly reduced by applying a two-part tow method. A comparison of the numerical and experimental results in the investigation demonstrates that the numerical simulation results are close to the experimental data, overall agreement between experimental and theoretical results is satisfactory. The results qualitatively verify the mathematical model of a two-part underwater towed system proposed by Wu and Chwang [Wu, J., Chwang, A.T., 2000. A hydrodynamic model of a two-part underwater towed system. Ocean Engineering 27 (5), 455–472].     

A New Fall-Rate Equation for T-5 Expendable Bathythermograph (XBT) by TSK

The accuracy of the manufacturer’s fall-rate equation for the T-5 Model of expendable bathythermograph (XBT) has been investigated based on about 300 collocated pairs of XBT-CTD (Conductivity-Temperature-Depth profiler) measurements in various climatological regions. We found that the equation systematically overestimates depth by about 5% for the T-5 produced by Tsurumi Seiki, Co. Ltd. (TSK), but almost no bias is associated with the T-5 produced by Sippican, Inc., in USA. The cause of this difference is not clear, because the two manufacturers’ T-5 probes are reported to have identical shape and weight in water. We propose a new fall-rate equation for the TSK T-5: z(t) = 6.54071t - 0.0018691t ², where z(t) is depth in meters at time, t, in seconds.     

On the fairing of resistance test data: A software-based approach

A fairing method is presented for giving a good representation of ship model resistance test data, which in general tend to exhibit a scattered trend and need to be smoothed appropriately. One component of the test data originates from the frictional resistance and can be formulated smoothly. The second component is the wave resistance which exhibits a specific harmonic character with peaks and troughs. In this method, this component is modelled using suitable harmonic cosine terms for obtaining the best representation of the corresponding resistance data. The performance of the method is then tested on two example cases, using the characteristic parameters of the sample data as input during the smoothing process.     

黄茅海河口湾现代动力地貌体系和冲淤过程分析

1980—1993年对黄茅海河口湾进行沉积物采样和水流测定及水深测量。根据水动力和地形条件，冲淤分析及Mclaren模型研究河口湾的动力地貌体系、冲淤特征和现代沉积物运移。结果表明：（1）水下地形主要为下泄流或上溯流控制的“深槽－槽沟－浅滩－湾口”的动力地貌体系，反映了河口湾“东进西出”的水流格局；（2）整个河口湾以淤积为主，只有崖门深槽有较明显的优势冲刷特征，并随着崖门深槽向海推移和河口湾“东进西出”水动力作用，黄茅海落潮三角洲相应向西南进积；（3）应用Mclaren模型揭示了黄茅海河口湾现代沉积物运移规律，同样反映了河口湾具有“东进西出”的运移趋势。     

注射生物胺对凡纳滨对虾鳃丝离子转运酶活力和血淋巴渗透压的影响

本文研究了注射生物胺(多巴胺、5-羟色胺)对凡纳滨对虾(Litopenaeus vannamei)离子转运酶活力和渗透压的影响。结果表明:注射生物胺(DA、5-HT)对凡纳滨对虾鳃丝总AT- Pase、Na -K -ATPase、V-ATPase活力的影响均显著(P<0．05)．而对HCO3--ATPase活力和渗透压的影响不显著(P>0．05),而对照组渗透生理指标无明显变化。注射DA组AT- Pase、Na -K -ATPase活力在6h内呈峰值变化,分别在1h、3h达到最大值,在6-12h时稳定在对照组水平,12h后逐渐升高,于36h时达到最大值后降低,而V-ATPase活力在实验时间内呈阶段式升高现象,在36h达最大值后降低;注射5-HT组ATPase、Na -K -ATPase活力随取样时间增加而降低．至9h达到最小值,9h后趋于稳定,V-ATPase活力在48h内与对照组差异不显著。注射DA、5-HT处理组血淋巴渗透压分别在6h、9h内呈明显的峰值变化．分别在1h、6h达到最大值,然后分别在6h、9h后趋于稳定,并恢复至对照组水平。     

用Matlab中的Neural Network Toolbox仿真赤道东太平洋SST的预报模型

基于ＮＣＥＰ／ＮＣＡＲ再分析资料和ＣＯＡＤＳ海洋资料中的全球月平均海平面气压场、８５０hPa纬向风场及海洋温度场，利用Ｍatlab中的Ｎeural Network Toolbox仿真环境和ＢＰ模型改进算法比较准确地仿真和反演出了南方涛动指数、赤道纬向风指数和滞后的赤道东太平洋海温之间的动力结构和预报模型。该模型具有很好的拟合精度和可行的预报效果。可在一定时效内预测赤道东太平洋月平均海温的变化趋势。由于所建系统是具有直接因果关系的预报模型，因此不仅可直接用于预测，而且可有效避免类拟非线性微分方程组在积分过程中由于对初值敏感性而可能产生的对预报结果的不确定性。     

台风"杜鹃"影响期间福建大风天气的特点及成因

台风“杜鹃”影响期间，福建大风天气出现的比较早，而且范围比较大．本文通过对天气尺度的环境场、具有代表性的单站资料的对比分析，寻求大风出现的原因．还深入利用MM5模式对台风的气压场、风场、流场等进行数值模拟，分析结果表明台湾地形倒槽的形成对此次大风天气出现有着重要的作用，此外从流场分型的角度进一步分析台湾地形倒槽的形成原因．     

Th sorption and export models in the water column: A review

Over the past few decades, the radioisotope pair of ²³⁸U / ²³⁴Th has been widely and increasingly used to describe particle dynamics and particle export fluxes in a variety of aquatic systems. The present paper is one of five review articles dedicated to ²³⁴Th. It is focused on the models associated with ²³⁴Th whereas the companion papers (same issue) are focused on present and future methodologies and techniques (Rutgers van der Loeff et al.), C / ²³⁴Th ratios (Buesseler et al.), ²³⁴Th speciation (Santschi et al.) and present and future applications of ²³⁴Th [Waples, J.T., Benitez-Nelson, C.R., Savoye, N., Rutgers van der Loeff, M., Baskaran, M., Gustafsson, Ö., this issue. An Introduction to the application and future use of ²³⁴Th in aquatic systems. Marine Chemistry, FATE special issue]. In this paper, we review current ²³⁴Th scavenging models and discuss the relative importance of the non-steady state and physical terms associated with the most commonly used model to estimate ²³⁴Th flux. Based on this discussion we recommend that for future work the use of models should be accompanied by a discussion of the effect that model and data uncertainty have on the model results. We also suggest that future field work incorporate repeat occupations of sample sites on time scales of 1–4 weeks in order to evaluate steady state versus non-steady state estimates of ²³⁴Th export, especially during high flux events (> ca. 800 dpm m^− 2 d^− 1). Finally, knowledge of the physical oceanography of the study area is essential, particularly in ocean margins and in areas of established upwelling (e.g., Equatorial Pacific). These suggestions will greatly enhance the application of ²³⁴Th as a tracer of particle dynamics and flux in more complicated regimes.