Study of colloidal phosphorus variation in estuary with salinity

The variation of colloidal phosphorus with salinity in estuary was studied in this paper, which was compared with those of particle and truly dissolved phosphorus with the purpose of ˉnding out the similarities and di?erences between their behaviors in the estuary. Distribution patterns of phospho- rus in particle, colloidal and truly dissolved phase at di?erent salinities and their relationships with suspended particulate matter were also studied to understand the transformation and transporta- tion of colloidal phosphorus in estuarine area. The result showed that the concentrations of total colloidal phosphorus, organic colloidal phosphorus and inorganic colloidall phosphate all descended from river-end to sea-end, illustrating their terrigenous source. Ratios of organic to total colloidal phosphorus decreased with salinity's increment, indicating that organic phosphorus was in°uenced by scavenging process of colloid more signiˉcantly as compared with inorganic phosphate. Both of the phosphorus variation tendencies and the proportion between organic and inorganic phosphorus in colloidal phase was similar with that in truly dissolved phase while di?erent from that in particle phase.     

Characterization of humic substances in salt marsh soils under sea rush (Juncus maritimus)

Humic substances (HS) from salt marsh soils were characterized and the relationships among HS composition and some geochemical factors were analysed. For this, three salt marshes with the same vegetation cover (Juncus maritimus), but with different geochemical characteristics, were selected. The qualitative characterization of the soil humic acids and fulvic acids was carried out by elemental analysis, FTIR spectroscopy, fluorescence spectroscopy and VACP/MAS ¹³C NMR spectroscopy.HS from salt marsh soils under sea rush (Juncus maritimus) displayed some shared characteristics such as low degree of humification, low aromatic content and high proportion of labile compounds, mainly polysaccharides and proteins. However, although the three salt marsh soils under study were covered by the same type of vegetation, the HS showed some important differences. HS composition was found to be determined not only by the nature of the original organic material, but also by environmental factors such as soil texture, redox conditions and tidal influence. In general, an increase in the humification process appeared to be related to aerobic conditions and predominance of sand in the mineral fraction of the soil, while the preservation of labile organic compounds may be associated with low redox potential values and fine soil texture.     

Advanced characterization of marine dissolved organic matter by combining reversed-phase liquid chromatography and FT-ICR-MS

Marine dissolved organic matter (DOM) was separated by reversed-phase (RP) liquid chromatography method and analyzed with fluorescence/absorption detection and Fourier transform ion cyclotron mass spectrometry (FT-ICR-MS). The three key characteristics of the RP method are: (a) The C18-RP column chosen provides enhanced separation when the aqueous phase is 100% buffer-free water, and it does not degrade over time; (b) the water eluent adjusted to pH 7 significantly improves the resolution of water soluble compounds; (c) the initial flow maintained at low levels improves the separation of polar compounds. In samples, containing “fresh” DOM, specific peaks were detected, which were absent in “old” DOM samples. The combination with size exclusion chromatography (SEC) also demonstrated the relation between polarity and molecular size of DOM. FT-ICR-MS was applied to evaluate the quality of separation on a molecular scale demonstrating that physico-chemical characteristics of DOM can be related to molecular formulas. Sample extracts were separated into 4 preparative fractions, and a large suite of the identified molecular formulas only occurred in specific fractions. This is an important basis for the application of further analytical techniques in order to perform a more target-oriented analysis aiming at the determination of source and process biomarkers for DOM.     

Determination of experimentally enriched15N in nitrate nitrogen based on an improved method of azo dye formation

A method has been developed for determination of¹⁵N isotope ratio in nitrate nitrogen, which is a major analytical step in tracer experiments for studies of nitrate metabolism in the marine environment. The method is based on diazotization of nitrite with sulfanilic acid following reduction of nitrate to nitrite by a cadmium-copper column. The diazonium compound is then subject to the azo coupling reaction with 2-naphthol, and the azo dye formed is extracted by a solid phase extraction column. The dye eluted from the column is collected, and total nitrogen and¹⁵N content of the dye are determined by mass spectrometry. Sulfanilic acid can also remove preexisting nitrite by heating the sample under acidic conditions before passing through the cadmium-copper reduction column. The average recovery of nitrate nitrogen was 86%. A procedure for reducing the background nitrogen that derives from the analytical operations has been developed; background nitrogen was limited to about 0.25 μg-atomN. The variation in the background nitrogen levels reflects the range of error in¹⁵N determination of nitrate nitrogen by this method. Application of the present method to a¹⁵NO₃ ⁻ isotope dilution experiment for determination of nitrification rate in sea water is demonstrated.     

Wave direction analysis from data buoys

This paper describes a new procedure of directional wave analysis from pitch-roll buoy measurements. The two previous procedures adopted by the National Data Buoy Center (NDBC) [Steele, K. E., Lau, J. C. K. and Hsu, Y.-H. L. (1985) Theory and application of calibration techniques for an NDBC directional wave measurement buoy. IEEE Journal of Oceanic Engineering OE-10(4), 382-396; Steele, K. E., Teng, C.-C. and Wang, D. W. C. (1992) Wave direction measurements using pitch-roll buoys. Ocean Engineering 19(4), 349-375] are relevant to our formulations. In these two studies, an estimate for the total phase shift of the sea surface displacement/slope spectra from the measured buoy heave/pitch and heave/roll spectra was calculated either by a weighted average method or a maximum heave/pitch quad-spectrum method. These two formulations were based on a fundamental assumption of symmetric hull-mooring effect on pitch and roll motions, which will never be true in the oceans. In the present study we essentially incorporate the basic formulations of NDBC, but calculate two estimates for this total phase shift.Examples of directional wave analysis from data measured by a 3 m diameter discus buoy during Typhoon Herb are presented in this paper. This data set was also analyzed by the weighted average method of Steele et al. (1985) which yielded unsatisfactory results of wave directions during severe wave climates.     

用13C-NMR光谱法研究几种红藻含硫半乳聚糖的结构特征

于１９８９年８月对采自青岛地区的５种红藻用冷水和热水分步提取或直接加热提取方法制备含硫半乳聚糖，对其多糖进行的＾１３Ｃ－光谱分析结果表明，扁江蓠多糖主要由琼胶糖和６－硫酸基－琼胶糖组分构成，海萝和多管藻多糖主要由６－硫酸基－琼胶糖组成，并含微量琼胶糖前体，６－ＯＣＨ３－琼胶糖组分，此两种藻的热水提取物中还含有微量２－ＯＣＨ３－琼胶糖，三叉仙菜的热水提取多糖主要由琼胶糖的前体组成；从海膜多糖谱图的信     

An Approximate Method for Calculation of Fluid Force and Response of A Circular Cylinder at Lock-in

In this paper, equations calculating lift force of a rigid circular cylinder at lock-in in uniform flow are deduced in detail. Besides, equations calculating the lift force on a long flexible circular cylinder at lock-in are deduced based on mode analysis of a multi-degree freedom system. The simplified forms of these equations are also given. Furthemore, an approximate method to predict the forces and response of rigid circular cylinders and long flexible circular cylinders at lock-in is introduced in the case of low mass-damping ratio. A method to eliminate one deficiency of these equations is introduced. Comparison with experimental results shows the effectiveness of this approximate method.     

海平面上升导致潮波系统变化的机理(Ⅱ)——基于数值模拟的研究

利用潮波基本方程数值模拟了湾口朝南的不同矩形海湾和曲折岸线海湾中海平面上升引起的潮位振幅和位相变化.根据矩形海湾中的模拟结果可以看出,如果忽略潮波方程中的非线性项,潮位振幅变化△R和位相变△θ的空间分布特征与基于理论模型在矩形海湾所获得的特征相当接近;当考虑方程中的非线性项时,不包括较深的海湾,正负△R的分界线变成1条通过无潮点附近的封闭曲线,而不是线性情况下的1条通过无潮点附近大致东西走向的曲线.另外也发现,在曲折岸线的海湾内,如果忽略非线性项,正负△R的分界线是1条通过2个无潮点的半环状的曲线,当考虑非线性项时,出现2条正负的分界线,它们是通过各自无潮点附近的封闭曲线.就△R的强度来说,非线性项使得正△R的强度减弱,在较浅水海湾中的△R强度大于较深水海湾中的强度,海底横向倾斜的海湾中的莫玆强度大于较浅水海湾中的强度.△R最强的区域位于无潮点附近的一段△R分界线之两侧.     

Dietary shift and feeding intensity of <Emphasis Type="Italic">Stenobrachius leucopsarus</Emphasis> in the Bering Sea

The food habits of the dominant myctophid Stenobrachius leucopsarus were examined in the central basin of the Bering Sea in relation to oceanographic conditions, in summer 2002 and 2003 and spring 2006. S. leucopsarus exhibited an ontogenetic and seasonal dietary shift. In spring, small fish (≤40 mm) preyed mainly on Neocalanus flemingeri/plumchrus whereas large fish fed mainly on Neocalanus cristatus. In summer, small fish preyed mainly on Metridia pacifica whereas large fish fed mainly on euphausiids (Thysanoessa spp.). In the summer of 2003, when water temperature in the epipelagic layer (≤100 m) was warmer, reflecting the prevalence of the Alaskan Stream, small-sized S. leucopsarus showed a higher stomach content index, perhaps reflecting the greater abundance of M. pacifica. Thus, the present study shows that the physical variability in the epipelagic layer affects not only diets but also feeding performance of micronekton.     

Improving the Definition and Algorithms of China’s Coastline Considering the Diversity of Tidal Characteristics

Two commonly used definitions of coastline are compared and analyzed in this article. To build a more perfect digital tide-coordinated coastline model in China, we should first solve the problems of the existing definition and unclear algorithms of China’s coastline. On the basis of defining the coastline in China as the intersection of coastal land and the mean high water spring (MHWS), it should be further revised, and defined either as an intersection of coastal land and the MHWS or as a mean higher high water level (MHHWL) considering the diversity of tidal characteristics in China Sea areas. Since MHWS is related to the extreme of the lunar phase and MHHWL is related to the extreme of the angle of the lunar declination elevation, the algorithms to obtain them are more complicated than that of mean high water (MHW) used in the United States. We should consider the specific law of tidal movement in different tide types of the predominantly semidiurnal or diurnal sea areas in China when studying the precise algorithms for MHWS and MHHWL. Several experiments show that the difference between the two types of calculations of coastal height, such as water level observations and tidal predictions, is within centimeter level. The research results of this article have a certain reference value for countries with similar coastline features as in China.