北极斯瓦尔巴群岛冰川流域可培养细菌的系统发育多样性及固氮菌初步研究

从北极斯瓦尔巴群岛冰川流域的上、中、下游采集了5种代表性土壤和冰川融水样品(MT、AT、TN、BT、MS),采用常规LB和严格无氮培养基低温培养方法,对其中的可培养细菌和潜在固氮细菌进行了总菌计数以及菌株的富集、分离与纯化。经菌落菌体形态观察、16S r RNA基因初步测序排重、固氮基因nif H的PCR检测,分别选取48株可培养分离细菌和31株潜在固氮菌进行了基于16S r RNA基因全序列测定的系统发育分析。目的是初步了解该特殊生境中可培养细菌(包括潜在固氮菌)的多样性及其分布状况,为进一步研究它们在此生境中的生态功能奠定基础。实验结果表明,可培养细菌在5个采集样品中的总菌数为BTTNATMT=MS,严格无氮培养基分离菌总菌数为BTATMTTNMS。48株可培养代表菌分属于Proteobacteria、Actinobacteria、Bacteroidetes、Firmicutes 4个门的7纲、14属之中,它们在5个样品中的多样性程度为BT(6纲、6属)MT(4纲、6属)AT、MS(均为3纲、3属)TN(2纲、3属),优势菌群主要分布在γ-proteobacteria纲的Pseudomonas属(20.8%,集中在AT、BT、MS样品中)和β-proteobacteria纲的Janthinobacterium属(35.4%,集中在MS样品中),表现出一定程度的系统发育多样性。潜在固氮菌实验结果表明,能在严格无氮培养基上生长且可检测到nif H的31株代表菌具有极为单一的系统发育多样性。其中90.32%菌株集中在γ-proteobacteria纲的Pseudomonas属,仅在MT和MS样品中有3株菌分布在α-proteobacteria纲的Rhizobium属、β-proteobacteria纲的Janthinobacterium属和Actinobacteria纲的Arthrobacter属,它们与分离自其他极端环境的相关标准菌株具有高度16S r RNA基因同源性。另外,本实验还发现有8株菌的16S r RNA基因序列与相关标准菌株的相似度低于98.50%,推测它们具有成为该特殊生境下新种的潜质。     

两株北极褐藻DNA条形码序列的记录

2012年第5次北极科学考察期间,分别在白令海海域和冰岛附近海域各采集到1株褐藻。描述了其形态学特征,并利用多种DNA条形码序列对其进行了分子鉴定。形态学和基于ITS2序列构建的系统进化树均表明白令海北部海域采集到的AE5-8为孔叶藻亚种Agarum clathratum subsp.Clathratum,这一亚种起源于日本北海道,经过阿拉斯加到北美的东西海岸都有分布。形态学特征及ITS序列和cox1序列均表明在冰岛附近海域采集到的AE5-12是瘤状囊叶藻(Ascophyllum nodosum),瘤状囊叶藻在北大西洋沿岸属于常见种,但是北太平洋海岸未见其分布的报道。     

北极巴伦支海和拉普捷夫海海冰中细菌和微藻的垂直分布

通过计算机图像测定系统测定、计算获得海冰细菌的大小、面积和碳含量,研究了北极巴伦支海和拉普捷夫海的浮冰中细菌大小、丰度和生物量的垂直分布及其与海冰微藻生物量的关系.细菌在冰心中是非均匀分布的,至少有两种不同分布类型:第一类有单一的丰度峰值,在冰心底部或在冰心中部;第二类有两个丰度峰值.海冰中细菌的丰度为0.4×105～36.7×105个/cm3.不同类型海冰中的细菌大小变化极大,在多年冰中,最大的细菌与叶绿素a最大值出现在同一层,而在一年冰中,细菌大小几乎没有垂直变化.整柱冰心的细菌生物量变化为19.2～79.2mg/cm2,细菌与海冰微藻生物量之比为0.43～10.00.对固定冰比较和研究的结果发现,海冰冰心中细菌大小、丰度和生物量的垂直分布差别极大.据此,分析了对目前海冰研究采样方法的局限性,并提出了规范采样方法的设想.     

长链烯酮及U37k值在北极海洋古温度的应用研究

在北极楚科奇海和白令海表层沉积物中检出长链烯酮化合物,研究结果表明楚科奇海和白令海沉积物中长链烯酮以C_37:3甲基酮占优势,C₃₇～C₃₉不饱和烯酮丰度变化顺序为C₃₇>C₃₈>C₃₉.根据∑C₃₇/∑C₃₈比值,所检出的长链烯酮母质生物主要是颗石藻(Emiliania huxleyi).应用U₃₇k和U₃₇k'标准校正关系式估算了表层海水古温度,其中U₃₇k'估算值为4.147～5.706℃(平均为5.092℃).     

Some parameters in arctic sea ice dynamics from an expedition in the summer of 2003

1Introduction Seaiceoccupiesthemainpartofthesurfaceof theArcticOcean.ThefocusoftheSecondChineseNa- tionalArcticResearchExpedition(CHINAE-2003) wastounderstandthevariationsofarcticmarineenvi- ronmentsandtheseaiceeffectsontheclimatechanges ofglobalextent,inmiddleandlowerlatitudesareas, especiallyinChina.Therefore,thejointsea-ice-airob- servationforseaicestudieswasoneofthekeypro- jectsinCHINARE-2003.Theinvestigatedareacov- ered3000kmfromsouthtonorthand900kmfrom westtoeast.Seventemporali…     

Arctic sea ice distribution in summer based on aerial photos

1Introduction TheArcticOceanisoneoftheimportantcold sourcesontheearth,whichaffectsglobalclimateand oceancirculationseriously.Itsinteractionwithglobal climatesystemisrepresentedbyseaice,whichisthe mainfeatureonthesurfaceoftheArcticOcean(Aa- gaard,etal.,1989).Firstly,seaiceplaysapivotalrole intheheatandmassbalanceonthesurfaceoftheArc- ticOcean.Seaicenotonlyobstructstheheatexchange betweenatmosphereandocean,butalsoreflectsthe mostofthelocalsolarradiationbacktotheatmo- spherebecauseofitshighalb…     

Extreme Cold Events in North America and Eurasia in November-December 2022: A Potential Vorticity Gradient Perspective

From 17 November to 27 December 2022, extremely cold snowstorms frequently swept across North America and Eurasia. Diagnostic analysis reveals that these extreme cold events were closely related to the establishment of blocking circulations. Alaska Blocking(AB) and subsequent Ural Blocking(UB) episodes are linked to the phase transition of the North Atlantic Oscillation(NAO) and represent the main atmospheric regimes in the Northern Hemisphere. The downstream dispersion and propagation of Rossby...     

Interdecadal correlation of solar activity with Tibetan Plateau snow depth and winter atmospheric circulation in East Asia

Studies on the impact of solar activity on climate system are very important in understanding global climate change. Previous studies in this field were mostly focus on temperature, wind and geopotential height. In this paper, interdecadal correlations of solar activity with Winter Snow Depth Index (WSDI) over the Tibetan Plateau, Arctic Oscillation Index (AOI) and the East Asian Winter Monsoon Index (EAWMI) are detected respectively by using Solar Radio Flux (SRF), Total Solar Irradiance (TSI) and Solar Sunspot Number (SSN) data and statistical methods. Arctic Oscillation and East Asian winter monsoon are typical modes of the East Asian atmospheric circulation. Research results show that on interdecadal time scale over 11-year solar cycle, the sun modulated changes of winter snow depth over the Tibetan Plateau and East Asian atmospheric circulation. At the fourth lag year, the correlation coefficient of SRF and snow depth is 0.8013 at 0.05 significance level by Monte-Carlo test method. Our study also shows that winter snow depth over the Tibetan Plateau has significant lead and lag correlations with Arctic Oscillation and the East Asian winter monsoon on long time scale. With more snow in winter, the phase of Arctic Oscillation is positive, and East Asian winter monsoon is weak, while with less snow, the parameters are reversed. An example is the winter of 2012/2013, with decreased Tibetan Plateau snow, phase of Arctic Oscillation was negative, and East Asian winter monsoon was strong.     

The Diavik Waste Rock Project: Geochemical and microbiological characterization of low sulfide content large-scale waste rock test piles

Two experimental waste-rock piles (test piles), each 15 m in height × 60 m × 50 m, were constructed at the Diavik diamond mine in Northern Canada to study the behavior of low-sulfide content waste rock, with a similarly low acid-neutralization potential, in a continuous permafrost region. One test pile with an average of 0.035 wt.% S (<50 mm fraction; referred to as Type I) and a second test pile with an average of 0.053 wt.% S (<50 mm fraction; referred to as Type III) were constructed in 2006. The average carbon content in the <50 mm fraction of waste rock in the Type I test pile was 0.031 wt.% as C and in the Type III test pile was 0.030 wt.% as C. The NP:AP ratio, based on the arithmetic mean of particle-size weighted NP and AP values, for the Type I test pile was 12.2, suggesting this test pile was non-acid generating and for the Type III test pile was 2.2, suggesting an uncertain acid-generating potential. The Type I test pile maintained near-neutral pH for the 4-year duration of the study. Sulfate and dissolved metal concentrations were low, with the exception of Ni, Zn, Cd, and Co in the fourth year following construction. The pore water in the Type III test pile contained higher concentrations of SO₄²⁻ and dissolved metals, with a decrease in pH to <4.7 and an annual depletion of alkalinity. Maximum concentrations of dissolved metals (20 mg L⁻¹ Ni, 2.3 mg L⁻¹ Cu, 3.7 mg L⁻¹ Zn, 35 μg L⁻¹ Cd, and 3.8 mg L⁻¹ Co) corresponded to decreases in flow rate, which were observed at the end of each field season when the contribution of the total outflow from the central portion of the test pile was greatest. Bacteria were present each year in spite of annual freeze/thaw cycles. The microbial community within the Type I test pile included a population of neutrophilic S-oxidizing bacteria. Each year, changes in the water quality of the Type III test-pile effluent were accompanied by changes in the microbial populations. Populations of acidophilic S-oxidizing bacteria and Fe-oxidizing bacteria became more abundant as the pH decreased and internal test pile temperatures increased. Irrespective of the cold-climate conditions and low S content of the waste rock, the geochemical and microbiological results of this study are consistent with other acid mine drainage studies; indicating that a series of mineral dissolution–precipitation reactions controls pH and metal mobility, and transport is controlled by matrix-dominated flow and internal temperatures.     

基于AMSR-E遥感数据应用强度比参数确定多年冰的方法探讨

研究发现,AMSR-E的垂直极化的18.7 GHz(V18.7)和36.5 GHz(V36.5)的亮温比值在一年冰覆盖区域主要是相应频段的海冰微波发射率之比,而在多年冰覆盖区域受海冰微波发射率和海冰温度共同影响,并且海冰年龄越大亮温比值也越大。应用强度比参数可以比较好地确定冬季一年冰与多年冰之间的阈值,其中,在该阈值处,强度比梯度达到最大。该阈值呈现明显的季节性变化规律,在冬季阈值比较稳定,而在夏季受海水的影响变化范围比较大。应用强度比方法确定的多年冰范围,与NASA Team2(NT2)方法相比在大西洋扇区差异非常小;而在太平洋扇区出现比较大的差异。对比发现强度比法确定的多年冰范围一般大于NT2法。