双壳贝类CK1基因家族的鉴定及表达分析

酪蛋白激酶1 (CK1)是一种重要的蛋白激酶家族,其在DNA损伤应答和修复、细胞增殖和凋亡、胚胎发育和稳态等重要的生物学过程中都具有复杂多样的调控作用。为了理解CK1基因家族在双壳贝类中的特征、进化及生物学功能,实验采用比较基因组学及生物信息学的方法对双壳贝类CK1基因家族进行了鉴定分析,通过虾夷扇贝高温应激实验,研究了CK1基因家族在高温应激时的表达规律。结果显示,在虾夷扇贝、栉孔扇贝、长牡蛎与侏儒蛤中均存在CK1α,CK1αlike,CK1δ,CK1γ3,并发现CK1ε,CK1γ1,CK1γ2在双壳贝类中发生了丢失。时空表达分析发现,双壳贝类CK1基因均在胚胎发育早期集中表达,并以多细胞/囊胚期为界呈现出母源/合子特异性表达模式。CK1为关键基因的基因共表达模块显著富集在错配修复、核苷酸剪切修复等相关通路上,暗示了CK1基因在双壳贝类胚胎发育过程中参与调控DNA损伤修复过程,从而维持早期胚胎发育时的基因组稳定性。双壳贝类CK1基因在成体中呈现组织特异的表达模式,主要在鳃和雄性性腺中具有相对较高的表达量。虾夷扇贝受到高温应激后,其鳃中PyCK1α, PyCK1αlike和PyCK1δ...     

Tracing dissolved organic matter cycling in the eastern boundary of the temperate North Atlantic using absorption and fluorescence spectroscopy

Apparent oxygen utilization (AOU), dissolved organic carbon (DOC), coloured dissolved organic matter (CDOM) absorption spectra, and CDOM fluorescence characteristic of aromatic amino acids (excitation/emission 280 nm/320 nm; F(280/320)) and marine-humic like substances (320 nm/410 nm; F(320/410)) were measured in full depth during a cruise in the temperate Eastern North Atlantic (ENA). An optimum multi-parameter (OMP) inverse method was run to calculate water mass proportion-weighted average (archetypal) concentrations of these chemical parameters for all water masses and samples. Archetypal concentrations retain the variability due to water mass mixing and basin scale mineralization from the water mass formation sites to the study area. Conversely, the difference between measured and archetypal concentrations, retain the variability due to dissimilarities in mineralization processes within the study area. Our analysis indicates that DOC supported 26±3% of the AOU in the dark temperate ENA and that basin scale processes occurring at and from the formation area of the water masses explained 63% of the total DOC variability. Our data also suggests that DOC remineralized at the basin scale was of lower molecular weight, and with a lower proportion of fluorescent aromatic amino acids than found within the study area. The relationship between the absorption coefficient at 254 nm (a_CDOM(254)) and AOU indicates that a_CDOM(254) was consumed during organic matter remineralization in the dark ocean, with 55% of the variability being explained by basin scale processes. The relationships of F(320/410) with AOU and DOC confirmed that marine humic-like substances are produced by microbial degradation processes, at a rate of 6.1±0.9×10⁻³ mg equivalents of QS mol AOU⁻¹. Our results also indicate that basin-scale remineralization processes account for 85% of the total variability of F(320/410), emphasizing that large scale processes control the formation of humic-like substance in the dark ENA.     

Analysis of the spatial variation of heavy metals in the Guadiana Estuary sediments (SW Iberian Peninsula) based on GIS-mapping techniques

This work reveals the usefulness of the GIS (Geographic Information Systems) mapping techniques to show the distribution of pollutants along an estuarine environment, as the final stage of a thorough study. In the case of study, the environmental quality of the sediments in the Guadiana river estuary was determined by means of a complete geochemical characterization consisting on the calculation of enrichment factors for the most important metals and metalloids (compared with the local background of non-contaminated sediments). The obtained results were depicted in “enrichment distribution maps” which evidenced a distribution of the elements in two groups: Group-I, elements with natural origin (Al, Fe, Mn, Co, and Cr) distributed homogeneously along the basin, and Group-II, elements associated to anthropic origin (As, Cd, Cu, Pb, Ni and Zn) with clear punctual sources besides a high concentration all over the estuary. The enrichment factors for the elements of Group-II are indicative of the existence of a noticeable diffuse historical mining pollution associated with the acid mine drainage generated in the internal zones of the basin, which could overlap minor pollution inputs from other human activities. The obtained results lead us to a reconsideration of the traditionally thought “unpolluted” environment when it was compared to nearby estuaries.     

Effects of wave–current interaction on the current profile

Wave–current laboratory experiments have shown that the logarithmic current profile observed in pure current flows is modified due to the presence of waves. When waves propagate opposite the current, an increase in the current intensity is achieved near the mean water level, while a reduction is obtained for following waves and currents. With the aim of analyzing these nonlinear effects along the whole water column, an Eulerian wave–current model is presented. In contrast to previously presented wave–current models, the present is able to include the variation of the free surface elevation due to the wave motion and the effect of a non hydrostatic pressure field. Therefore it does not restrict its application to waves in shallow waters. Moreover, the model is able to simulate all the possible angles between waves and currents.     

下扬子下寒武统岩相古地理及烃源岩条件研究

以下扬子下寒武统为研究对象,利用野外露头、岩心、岩石微观分析、有机地球化学等资料,在地层对比基础上,开展了下扬子下寒武统岩相古地理研究及烃源岩条件评价。结果表明：下扬子下寒武统主要发育硅质岩、硅质泥页岩、碳质泥页岩、灰质泥页岩、泥灰岩、白云岩6种岩石类型;地层可划分为3段,其中安吉-石台-上海地区三段发育齐全,其他地区一段大部分缺失,二段存在不同程度缺失;沉积相主要发育盆地、陆棚、斜坡、台地边缘、局限台地5种类型;烃源岩主要分布于石台-安吉、滁州-盐城、德兴-桐庐盆地相及周边陆棚相,石台-安吉地区是烃源岩发育最有利区,泥岩厚度300~700m,TOC为3%~9%,平均4.5%~5.76%;德兴-桐庐地区泥岩厚度200~400m,TOC为3%~9%,平均4.96%;滁州-盐城凹陷泥岩厚度50~200m,TOC为2%~4%。下扬子下寒武统烃源岩干酪根类型全部为Ⅰ型,有机质组分主要为腐泥组,以藻类体为主,含少量腐泥无定形体。下扬子下寒武统烃源岩均处于过成熟演化阶段,总体上由皖南盆地区向两侧台地逐渐降低,九江、景德镇、黄山及开化淳安地区受火山岩影响Ro在4.0%以上;安庆-溧阳-南通以南,湖州-苏州以西处于盆地、陆棚及斜披部位,埋深大,Ro为3.0%~4.0%;潜山、南陵、无为、金坛及苏北中新生代凹陷由于后期埋深较大,Ro在3.0%以上;其他区域Ro为2.0%~3.0%。     

Spatiotemporal variation and mechanisms of temperature inversion in the Bay of Bengal and the eastern equatorial Indian Ocean

In the northern Bay of Bengal, the existence of intense temperature inversion during winter is a widely accepted phenomenon. However, occurrences of temperature inversion during other seasons and the spatial distribution within and adjacent to the Bay of Bengal are not well understood. In this study, a higher resolution spatiotemporal variation of temperature inversion and its mechanisms are examined with mixed layer heat and salt budget analysis utilizing long-term Argo（2004 to 2020） and RAMA（2...     

渤海湾渔业碳汇分析与预测初探

为了解渤海湾近十年来渔业碳汇能力,本研究汇总了渤海湾地区2010-2020年海水养殖的品种和产量。通过建立不同养殖品种固碳能力评估体系,计算了不同养殖品种对渔业碳汇的贡献和渤海湾近十年海水养殖固碳总量。结果表明,2010-2020年渤海湾海水养殖以池塘养殖为主要养殖模式,年固碳量从2010年的5.79万吨增至2020年的11.04万吨,十年内共计固碳96.0万吨,平均碳转化比为12.5%,其中山东省贡献超过65%。该探索对建立“渔业碳汇”计算的指标体系、科学发展碳汇渔业、提升海洋碳汇生态价值具有一定的指导意义。     

南海东北部海底沉积物波的形态、粒度特征及物源、成因分析

在南海东北部广泛发育沉积物波。通过高分辨率多波束数据、地震剖面以及重力柱状样,对沉积物波的形态特征、粒度特征、物源以及形成机制进行了分析。研究表明大致以台湾浅滩南海底峡谷为界,北侧为近北东向展布,南侧为近南北向展布。对其分布规律、地貌和形态特征及重力柱状样粒度分析表明这些沉积物波为浊流成因。沉积物波的发育与新生代晚期研究区的构造活动密切相关,自距今6.5 Ma以来台湾造山运动使台湾岛强烈抬升剥蚀,这些剥蚀物为研究区提供了大量的陆源物质,而在南海东北部陆坡区大量发育的峡谷-冲沟系统为陆缘物质向下陆坡的输送提供了良好的通道。研究区西侧的东沙隆起长期处于抬升剥蚀状态,这种抬升剥蚀也为研究区沉积物波的发育提供了部分物源。随着坡度的减缓,浊流沉积物开始堆积,在台湾浅滩南海底峡谷的北侧形成了展布方向与冲沟垂直的沉积物波,而在南侧由于台湾浅滩南海底峡谷发生转向,浊流从水道中漫溢出来,沉积物堆积下来,形成了与原先水道近于垂直的近南北向的沉积物波。     

Annual and Seasonal Variations of the Sea Surface Heat Fluxes in the East Asian Marginal Seas

Based on the twice-daily marine atmospheric variables which were derived mostly from the weather maps for 18 years period from 1978 to 1995, the surface heat flux over the East Asian marginal seas was calculated at 0.5°×0.5° grid points twice a day. The annual mean distribution of the net heat flux shows that the maximum heat loss occurs in the central part of the Yellow Sea, along the Kuroshio axis and along the west coast of the northern Japanese islands. The area off Vladivostok turned out to be a heat-losing region, however, on the average, the amount of heat loss is minimum over the study area and the estuary of the Yangtze River also appears as a region of the minimum heat loss. The seasonal variations of heat flux show that the period of heat gain is longest in the Yellow Sea, and the maximum heat gain occurs in June. The maximum heat loss occurs in January over the study area, except the Yellow Sea where the heat loss is maximum in December. The annual mean value of the net heat flux in the East/Japan Sea is −108 W/m² which is about twice the value of Hirose et al. (1996) or about 30% higher than Kato and Asai (1983). For the Yellow Sea, it is about −89 W/m² and it becomes −75 W/m² in the East China Sea. This increase in values of the net heat flux comes mostly from the turbulent fluxes which are strongly dependent on the wind speed, which fluctuates largely during the winter season. This revised version was published online in August 2006 with corrections to the Cover Date.