菲律宾蛤仔(Ruditapes philippinarum)漆酶型酚氧化酶的纯化及特性分析

采用非变性电泳结合特异性底物发色方法从菲律宾蛤仔血细胞中分离出一种酚氧化酶,研究了该酶的酶促反应动力学参数,金属离子、抑制剂对酶活的影响。结果表明,分离获得的酚氧化酶分子量约为563kDa;对底物邻苯二酚、左旋多巴(L-DOPA)、多巴胺、对苯二酚的米氏常数Km分别为0.42、1.60、1.79和1.76mmol/L;酶的活性在Mg2+、Zn2+、Mn2+、Fe2+、Cu2+和Ca2+浓度为5、10、20、30mmol/L时受到抑制;酶的活性可被抑制剂抗坏血酸、二乙基二硫代氨基甲酸钠(DETC)、半胱氨酸、亚硫酸钠、柠檬酸、乙二胺四乙酸二钠(EDTA)、叠氮钠和硫代尿素等抑制。根据底物特异性、抑制剂等结果,所分离获得的酚氧化酶属于漆酶型的含铜酚氧化酶。     

菲律宾蛤仔碱性磷酸酶的分离纯化及部分性质研究

经Tris-HCl缓冲液浸提、硫酸铵分级沉淀盐析、DEAE-Sepharose Fast Flow离子交换层析和Sephadex G-75凝胶过滤层析等步骤,从菲律宾蛤仔内脏团中分离得到碱性磷酸酶,SDS-PAGE显示为单一条带,提纯倍数为14.42,比活力达到131.08U/mg。酶学性质研究表明:该酶亚基分子量约44kD,最适反应温度为45℃,最适pH值为9.0,米氏常数Km为0.098mmol/L,最大反应速度Vmax为1.01μmol/(mL·min)。Ca2+、Mg2+对酶活力表现出显著的激活作用。     

Multi-scale variation characteristics of polar vortex at 10 hPa in the Southern Hemisphere

By use of 1948-2007 NCEP/NCAR reanalysis monthly geopotential data, a set of circulation indices are defined to characterize the polar vortex at 10 hPa in the Southern Hemisphere, including area-(S), intensity-(P) and centre position-(λc , φc) indices. Sea-sonal variation, interannual anomalies and their possible causes of 10 hPa polar vortex in the Southern Hemisphere are analyzed by using these indices, the relationship between 10 hPa polar vortex strength and the Antarctic Oscillation are analyzed as well. The results show that: (1) the polar region at 10 hPa in the Southern Hemisphere is controlled by anticyclone (cyclone) from Dec. to Jan. (from Mar. to Oct.), Feb. and Nov. are circulation transition seasons. (2) Intensity index (P) and area index (S) of anticy-clone (cyclone) in Jan. (Jul.) show a significant spike in the late 1970s, the anticyclone (cyclone) enhances (weakens) from ex-tremely weak (strong) oscillation to near the climatic mean before a spike, anticyclone tends to the mean state from very strong oscillation and cyclone oscillates in the weaker state after the spike. (3) There is significant interdecadal change for the anticyclone center in Jan., while markedly interannual variation for cyclone center in July. (4) The ozone anomalies can cause the interannual anomaly of the polar anticyclone at 10 hPa in the Southern Hemisphere in Jan. (positive correlation between them), but it is not related to the polar cyclone anomalies. (5) There is notable negative correlation between the polar vortex intensity index P and the Antarctic Oscillation index (AAOI), thus AAOI can be represented by P.     

中国大陆东南缘地震接收函数与地壳和上地幔结构

从2008-2011年,分别在中国大陆东南缘沿海和内陆两条NE向剖面上进行了宽频地震观测,利用记录到的远震波形资料提取得到1446个远震P波接收函数,用H-κ叠加扫描和CCP偏移叠加方法研究了中国大陆东南缘地壳及上地幔过渡带的结构及其变化特征.结合固定台网25个台站的H-κ结果,获得中国大陆东南缘(福建地区)地壳厚度从内陆到沿海逐渐减薄的图像:地壳从闽西北山区的33 km减薄到厦门沿海一带的29 km以下,平均地壳厚度为31.3 km,具有陆地向洋壳过渡的特征;地壳泊松比从内陆到沿海显示出分带特征,闽中西部内陆地区小于0.26,沿海地带高于0.26,且在断裂带的交汇区域表现为相对异常高值.地壳上地幔顶部(0~200 km)的CCP偏移叠加成像结果显示闽江断裂等NW向断裂深切Moho界面,在断裂两侧Moho面急剧抬升或下沉,产状改变,这些特征向内陆地区逐渐变得不明显.闽江等NW向断裂对研究区地壳厚度、地震等有明显控制作用.上地幔尺度(300~700 km)的CCP偏移叠加成像,未见410 km和660 km速度间断面突变和起伏异常,其绝对深度略大于IASP91模型的,上地幔转换带厚度正常(250±5 km),表明中国大陆东南缘上地幔转换带未受欧亚与菲律宾板块碰撞的明显影响,推断中国大陆东南缘及台湾海峡下方不存在俯冲板块,或俯冲前缘未扰动到410 km的深度.     

菲律宾三宝颜省巴哟铁矿类型及分布特征初探

巴哟磁铁矿矿化露头多,矿床小而散,属风化残坡积型,原生矿成因类型较复杂,成矿地质条件良好,铁矿找矿潜力较大,具大型富铁矿找矿远景。     

菲律宾地质构造及其区域成矿主要特征(二)

菲律宾属于太平洋板块向欧亚板块俯冲形成的岛弧构造体中的一环。它在地质构造演化历史上,前中生代为地槽区,可能在三叠纪末受构造变动影响(有人认为受全球性特提斯运动影响)构成了岛弧初形,晚中生代至古近纪末受太平洋构造变     

菲律宾土地管理机构概况

菲律宾环境和自然资源部是菲律宾国土资源的主管部门,而其下属的土地管理局为该国主要的土地管理机构。本文介绍了菲律宾环境和自然资源部及其下属的土地管理局的机构概况,以及菲律宾与土地管理相关的法律法规。     

菲律宾达沃砂金矿中金汞矿物研究及其找矿意义

本文系统研究了菲律宾棉兰老岛达沃砂金矿的矿物组合、金汞矿物的矿物学特征,认为随着金汞矿物中汞含量成分增加,金-汞系列矿物反射力具有由强-弱-强的变化特征,同时矿物硬度也随着减少。但是,当汞含量增加到相当比例时,尤其是晶体结构为等轴晶系时,其硬度却逐渐变大。笔者研究发现,金汞齐和r-汞金矿只是作为自然金或含汞自然金的矿物...     

菲律宾蛤仔苗种土池中间培育及越冬生产实验

利用2．6hm^2和3．13hm^2 2个养虾池,经过池底清淤、底质改良、敌害清除、培养用水处理、繁殖基础饵料生物等工作后,分别移入在室内培育的菲律宾蛤仔（Ruditapes philippinarum（Adams et Reeve））壳顶后期幼虫和双水管稚贝各80亿粒和9．7亿粒,经土池中间培育、越冬后,生产出2000-3000粒／500g幼贝7．9亿粒。自移入幼虫和稚贝到幼苗出池,成活率分别为4．5％和44．3％,达到了较高水平的中间培育、越冬成活率。     

根据多卫星高度计海面高数据推算南中国海及菲律宾海域重力异常

t Gravity anomalies on a2.5 ×2.5 arc-minute grid in a non-tidal system were derived over the South China and Philippine Seas from multi-satellite altimetry data. North and east components of deflections of the vertical were computed from altimeter-derived sea surface heights at crossover locations, and gridded onto a 2.5 × 2.5 arc-minute resolution grid. EGM96-derived components of deflections of the vertical and gravity anomalies gridded into 2.5 × 2.5 arc-minute resolutions were then used as reference global geopotential model quantities in a remove-restore procedure to implement the Inverse Vening Meinesz formula via the 1D-FFT technique to predict the gravity anomalies over the South China and Philippine Seas from the gridded altimeter-derived components of deflections of the vertical. Statistical comparisons between the altimeter-derived and the shipboard gravity anomalies showed that there is a root-mean-square agreement of 5.7 mgals between them.