Temporal and dimensional distribution of sulfate-reducing bacteria （SRB） groups and quantity in the sediments of Lake Erhai

The purpose of the thesis is to analyze the temporal and dimensional distribution of sulfate-reducing bacteria （SRB） groups and quantity in Lake Erhai. In April and September 2005, two sediment cores were collected from Lake Erhai. SRB groups were analyzed by PCR with six-groups primers designed according to the specific 16SrDNA sequence. FISH （fluorescence in-situ hybridization） was established with the oligonucleotide probe （SRB385） and utilized to analyze SRB quantity in the sediments. The results showed that in the sediments of Lake Erhai four SRB groups were detected except Desulfobacterium and Desulfobacter, meanwhile Desulfovibrio-Desulfomicrobium were detected only in autumn; different SRB groups had different temporal and dimensional distribution, and each group in autumn is distributed more widely than in spring; FISH used to count SRB in the sediments of fresh lake was set up successfully; the analysis of correlation between the sediment＇s depth and SRB quantity had statistical meaning （P〈0.05） . The result showed that SRB quantity showed a decreasing trend with increasing depth. Through the analysis of randomized block designed analysis of variance, the difference in SRB quantity between spring and autumn also had statistical meaning （P〈0.001）, which revealed SRB quantity in autumn was larger than in spring; the result of FISH showed that there were some SRB in the deeper sediments in which no above-mentioned six SRB groups were detected by PCR. SRB groups in the sediments of Lake Erhai were rich, and the quantities of SRB groups in autumn were larger than in spring; possibly there were uncultivable SRB groups in the sediments of Lake Erhai.     

Geochemistry of porewater and sulfate-reducing bacteria in sediment cores from the Chianan Plain, Taiwan

High concentrations of arsenic and humic substances in groundwater from the southwestern coastal plain of Taiwan were well known for their probable relationships with black-foot disease. In order to realize the relationships between the concentrations of humic substances and arsenic in this area, 24 well water samples were analyzed. After filtered through 0.45 μm glass fiber membrane in the field, samples were kept in the dark at 4℃ and then separated into six fractions with varying range of molecular weight （〈0.5, 0.5-1, 1-5, 5-10, 10-50 and 〉50 k Da） by ultrafiltration apparatus （Molecular/Por Stirred Cell system） in the lab. Concentrations of humic substances were measured by fluorescence spectrometer （HITACHI F-2000, ex=370, em=445） and arsenic by FIAS-AA （Perkin Elmer AAnalyst 100, FIAS-400）. On average, only 6.2% of the total arsenic in water existed in the fraction of 〉0.5 k Da. and the others were complexed with humic substances （including humic acid and fulvic acid）. The results demonstrated a distinct positive correlation between the concentrations of humic substances and arsenic.     

Synergistic effect of combining sulfate reducing bacteria and zerovalent iron permeable reactive barriers on the treatment of groundwater rich in uranium, sulfate and heavy metals

Uranium processing and mining activities that generate many contaminants, such as high concentrations of U （VI）, sulfate and heavy metals （Zn, Cu, Ni, etc）, may pose a serious threat to the groundwater resources. In recent years, considerable research has been conducted respectively on two kinds of permeable reactive barriers （PRB）, including zerovalent iron （ZVI） and sulfate reducing bacteria （SRB）, for in-situ removal of these pollutants from groundwater. However, little investigation has been carried out on the potential benefits of bioaugmenting ZVI barriers to enhance the elimination of the pollutants by combining ZVI with SRB systems. The main goal of this study was to conduct batch and column experiments to determine whether the combination of SRB and ZVI can function synergistically and accelerate the rate of pollutant removal. The results of anaerobic batch experiments demonstrated that although the integrated ZVI/PRB system itself has no ability to reduce and remove sulfate directly, SRB can utilize hydrogen gas produced during the slow process of ZVI corrosion as an electron donor to raise biomass yields significantly and accelerate reductive sulfate removal. In particular, ferrous cations produced as the byproduct of ZVI corrosion process reacted with hydrogen sulfide from sulfate reduction and formed iron-bearing sulfide precipitates, which can stimulate the growth of SRB and promote sulfate removal activity by eliminating the biotoxicity of hydrogen sulfide. It was also shown that secondary mineral products （pyrite/ferrous sulfide） formed as a consequence of microbial sulfate reduction and ZVI corrosion process can enhance the microbial precipitation of soluble U （VI） as insoluble uraninite（uranium dioxide）.     

Characterization of microbial communities associated with African desert dust and their implications for human and ecosystem health

Desert winds aerosolize several billion tons of soil-derived dust each year, including concentrated seasonal pulses from Africa and Asia. Huge dust events create an atmospheric bridge over continents and oceans, and eject a large pulse of soil-associated microorganisms into the atmosphere. These dust events might therefore have a role in expanding the biogeographical range of some microorganisms by facilitating rare long-distance dispersal events. The goal of this study is to characterize the microbes associated with African dust events and determine if they pose a risk to humans or downwind ecosystems. Air samples were collected by vacuum filtration in a source region （Mali, West Africa） during dust events and plated on R2A media to culture microorganisms. These organisms were compared to those in similar samples collected in the Caribbean during Saharan/Sahelian dust events. A high-volume liquid impinger is currently being tested in Barbados, collecting aerosol samples during African dust events. Over 100 bacteria and fungi （19 genera of bacteria and three genera of fungi） have been characterized from source region dust events.     

紫金牛生长土壤微生物和养分特性

研究了自然坡地坡上、坡中和坡下三种不同部位上紫金牛生长土壤的微生物特性和养分特性。结果表明，紫金牛只生长在坡下，紫金牛生长的坡下土壤中细菌比例最高、真菌比例最低，土壤全氮和有机质含量均高于对照的坡中和坡上。     

南极菌Pseudoalteromonas sp．S-15-13产胞外多糖的研究及其分子鉴定

本文对影响南极细菌S-15-13生长和胞外多糖产量的主要环境因子进行了研究,同时采用16S rDNA序列分析及系统发育分析方法对其进行了分子鉴定。结果表明：菌株S-15-13最佳产糖条件为：培养时间,56h；培养温度,8℃；碳源,1．0%葡萄糖；NaCl浓度,3．0%；pH,6．0-7．0。16S rDNA序列分析和系统发育分析表明,菌株S-15-13属于假交替单胞菌属(Pseudoaltero- monas)。     

早期煤化作用机制与有机质早期成烃演化

近年来，未熟和低熟油气田的发现、生物气藏的勘探和开发，极大地促进了对有机质早期转变的研究；而有机质早期转变机制的正确认识对于煤和干酪根中显微组分的成因、后期热演化都有着重要的影响。对早期煤化作用机制和有机质早期成烃作用的研究现状、进展及存在的问题进行了综述。     

上扬子台地晚震旦世灯影组中葡萄状-雪花状白云岩的成因意义

葡萄状 -雪花状白云岩是白云岩形成之后经淡水淋滤作用而成 ,葡萄内或雪花周围的有机物质不是原生藻 ,而是蓝绿藻经细菌腐解后形成的细菌集合体。该套白云岩的形成与沉积环境关系密切 ,与藻类多少无关。葡萄状白云岩富含有机物质 ,而雪花状白云岩孔洞发育 ,因此葡萄状 -雪花状白云岩既是生油层 ,又是良好的储集层 ,对其重新认识和定位 ,必将为寻找深部油气资源提供极为重要场所     

滇池农村固体废物和化肥流失治理试验研究

怎样合理处置农村固体废物和减少农田化肥流失一直是农村面源污染控制的难题之一。该研究将农村固体废物的处理与减少化肥施用措施有机结合，利用微生物复合发酵剂和农村固体废物生产活性肥料，开展了一系列农业种植试验和数据监测，在此基础上，提出了一套实用性强、效果好的控制农业固体废物和化肥流失污染的对策措施。     

北极海冰细菌产胞外酶及主要环境因子的初步研究

覆盖在北极海洋上的广阔海冰为其内部生存的微生物群落提供了一个独特的生境。研究表明 ,大量海冰细菌能够分泌产生胞外酶 ,其中产蛋白或脂质水解酶细菌的比例远高于产多糖水解酶的细菌。温度、盐度是直接影响海冰细菌生存与活力的 2个主要环境因子。 76%的产酪蛋白酶海冰细菌为低温菌 ,菌株只能在 <35°C条件下生长 ;而 98%的酪蛋白酶最适作用温度≥ 35°C ,其中 62 %的酶最适作用温度≥ 45°C。几乎所有的产酪蛋白酶海冰细菌都耐盐或嗜盐