海底爆破振动强度衰减规律及中华白海豚保护措施研究

为确保海底爆破施工所诱发的振动不对中华白海豚造成危害,基于量纲分析法对萨道夫斯基经验公式进行修正,建立适用于预测海底爆破质点峰值振动速度的相似准数方程。在此基础上根据现场试验数据进行非线性回归运算,得到相关参数并分析了海底爆破振动强度衰减规律。分析表明:海底爆破质点峰值振速主要由单段装药量、监测点距爆心水平距离、覆盖层厚度及海水深度等因素决定。将参数值分别代入萨氏公式及新建公式计算预测值并进行误差估计,其平均误差分别为20.3%和10.0%,新建公式提高了对质点振速预测的准确性。利用新建公式及超压内安全振速估算保护中华白海豚的安全距离并提出相应的保护措施,为同类工程施工提供理论参考依据。     

三疣梭子蟹几丁质酶基因(PtCht6)的克隆及其在免疫中的功能分析

几丁质酶(chitinase)在甲壳动物的蜕皮、消化和免疫等很多生物学过程中发挥重要功能,为深入探讨几丁质酶的免疫防御机制,本实验利用RACE技术克隆了三疣梭子蟹(Portunus trituberculatus)PtCht6基因。该基因cDNA全长2736bp,开放阅读框(ORF)2103bp,编码700个氨基酸,具有几丁质酶GH18家族典型特征。利用实时荧光定量技术分析PtCht6在三疣梭子蟹不同组织、不同蜕皮阶段、不同病原感染以及低盐胁迫(11)下的表达特征,结果显示:PtCht6在各组织中均有表达且在肝胰腺中的表达量最高;在不同蜕皮时期的肝胰腺中,其表达量由蜕皮后期(A/B)、蜕皮间期(C)、蜕皮前期(D)依次递减(P0.05);人工感染WSSV和副溶血弧菌后.PtCht6的表达量在肝胰腺中分别于12h、72h达到最大值,在血细胞中均于12h达到最大值,且相较于对照组,整体显著上调表达(P0.05);低盐胁迫能够显著抑制该基因的表达,最高抑制73倍(P0.05)。同时发现,低盐环境下感染WSSV后,该基因达到峰值的时间明显延迟(至少延迟12h.P0.05)。该研究结果预示PtCht6作为免疫因子参与三疣梭子蟹的病原防御,且低盐胁迫在一定程度上抑制了该基因的免疫功能。     

GPS变形监测动态数据处理中卡尔曼滤波的应用

介绍了卡尔曼滤波的基本原理,针对常规GPS变形监测数据处理中存在着不连续、非实时的缺点,结合卡尔曼滤波的特点,运用三峡实测数据对GPS变形监测动态数据处理中卡尔曼滤波的应用进行研究。并采用各点点位位移速度图对所建立的函数模型进行验证,同时对状态方程的建立及初始值的选取进行分析总结。     

White spot syndrome virus envelope protein VP124 involved in the virus infection

White spot syndrome virus （WSSV） is one of the major shrimp pathogens causing large economic losses to shrimp farming. In an attempt to identify the envelope proteins involved in the virus infection, purified WSSV virions were mixed with three antisera against WSSV envelope proteins （VP39, VP124 and VP187 ）, individually. And then they were injected intramuscularly into crayfish （Procambarus clarkii） to conduct in vivo neutralization assays. The results showed that for groups injected with virions only and groups injected with the mixture of virions and antiserum against VP124, the crayfish mortalities were 100% and 60% on the 8th day postinfection, individually. The virus infection could be delayed or neutralized by antibody against the envelope protein VP124. Quantitative PCR was used to further investigate the influence of three antisera described above on the virus infection. The results showed that the antiserum against VP124 could restrain the propagation of WSSV in crayfish. All of the results suggested that the viral envelope protein VP124 played a role in WSSV infection.     

Experimental Study of a Command Governor Adaptive Depth Controller for an Unmanned Underwater Vehicle

Unmanned Underwater Vehicles (UUVs) are increasingly being used in advanced applications that require them to operate in tandem with human divers and around underwater infrastructure and other vehicles. These applications require precise control of the UUVs which is challenging due to the non-linear and time varying nature of the hydrodynamic forces, presence of external disturbances, uncertainties and unexpected changes that can occur within the UUV’s operating environment. Adaptive control has been identified as a promising solution to achieve desired control within such dynamic environments. Nevertheless, adaptive control in its basic form, such as Model Reference Adaptive Control (MRAC) has a trade-off between the adaptation rate and transient performance. Even though, higher adaptation rates produce better performance they can lead to instabilities and actuator fatigue due to high frequency oscillations in the control signal. Command Governor Adaptive Control (CGAC) is a possible solution to achieve better transient performance at low adaptation rates. In this study CGAC has been experimentally validated for depth control of a UUV, which is a unique challenge due to the unavailability of full state measurement and a greater thrust requirement. These in turn leads to additional noise from state estimation, time-delays from input noise filters, higher energy expenditure and susceptibility to saturation. Experimental results show that CGAC is more robust against noise and time-delays and has lower energy expenditure and thruster saturation. In addition, CGAC offers better tracking, disturbance rejection and tolerance to partial thruster failure compared to the MRAC.     

Heterosis and heritability estimates for the survival of the Pacific white shrimp (Litopenaeus vannamei) under the commercial scale ponds

The aim of the present study is to detect the potential of the base population from diallel crosses of eight introduced strains of the Pacific white shrimp(Litopenaeus vannamei) for improving the yield. Heterosis and heritability were estimated for pond survival at commercial farm conditions for the base population that included 207 full-sib families from a nested mating design by artificial insemination. Among all the hybrids,the heterosis ranged from –11.37%(UA1×UA2) to 20.53%(UA3×SIN) with an average of 0.953%. The results showed that more than half of the hybrids(51.85%) have negative heterosis for survival rate, but most of the hybrids with positive heterosis have high estimates. The high proportion of negative heterosis for survival rate reminders us that the survival trait also should be considered in the crossbreeding program to avoid yield decrease. However, high positive heterosis manifested in most of the hybrids for survival indicates the usefulness of these hybrids for improving the survival to obtain higher yield by crossbreeding in this breeding program. The heritability estimate for pond survival was 0.092±0.043 when genetic groups were included in the pedigree, and it was significantly different from zero(P0.05). The results from this study also indicated that significant improvement for survival is possible through selection in L. vannamei.     

小波变换压制噪声在单道地震资料处理中的应用

海洋区域地质调查中,单道地震勘探以其高分辨率、方便快捷等优点成为揭示浅地层地质构造的重要手段之一.结合前人研究成果并出于保护信号的完整性,对低频部分的噪声采用带通滤波进行滤除,而对高频信号中的随机噪声采用小波阈值去噪方法进行压制处理.在小波阈值去噪过程中,通过比较选用最佳小波基函数sym5对地震剖面进行三层小波分解.结果表明:两种阈值去噪方法在带通滤波的基础上进一步提升了剖面的质量,使得反射层面变得连续可追踪.软阈值去噪滤掉了剖面上的较多信息,去噪效果较为彻底,硬阈值去噪则保留了较多的细节信息.     

Movement patterns of an endangered fishery species,Lithognathus lithognathus (Sparidae), and the role of no-take marine protected areas as a management tool

Understanding movement behaviour is essential for effective management of fishery species. Dart tags were used to study coastal movement patterns of white steenbras Lithognathus lithognathus, an overexploited seabream (Sparidae) in South Africa. In total, 6 962 fish (190–1 080 mm fork length) were tagged throughout the species’ distributional range, in four long-term fish-tagging programmes. The predominant behaviour recorded was residency, with relatively short-ranging movements. More than 60% of the 351 recaptured fish were recaptured within 1 km of their tagging site, some of which were at liberty in excess of three years. Most of the juveniles (93.9%), subadults (71.7%) and adults (64.0%) were recaptured within 10 km. Occasional long-distance movements of up to 800 km were recorded. Recapture distances were positively, but weakly, correlated with fish fork length (n = 257 fish measured at recapture; r² = 0.166, p < 0.001). Low levels of connectivity among coastal areas suggest that large-scale annual spawning migrations, as previously hypothesised for this species, are unlikely, which raises the possibility of multiple spawning sites. Seventy-seven percent of L. lithognathus tagged within three marine protected areas (MPAs) were recaptured within the same MPA, suggesting that area closures provide protection for L. lithognathus through the post-estuarine juvenile, subadult and adult life stages. We confirm that the country’s current network of coastal MPAs plays a vital role in sustaining this species, and suggest that additional closures, or otherwise substantial reductions in catch rates, are necessary for the species’ recovery.     

基于PSR 模型的厦门湾中华白海豚资源评价

中华白海豚(Sousa chinensis Osbeck,1765)为国家Ⅰ级重点野生保护动物,厦门湾作为其在中国的主要分布区之一,自20世纪80年代以来,海岸带开发活动和环境污染程度均不断加大,导致此海域中华白海豚的生存环境不断恶化,种群数量日趋减少,资源状况面临严重威胁。作者采用PSR(压力-状态-响应)模型框架,对厦门湾中华白海豚资源面临的主要环境压力、当前的资源状态以及采取的保护响应措施进行了一个较全面的评价,评价结果认为:围填海工程、船只交通、水下爆破作业和陆源排污等人类活动给厦门湾中华白海豚带来了生境缩小、身体伤害、环境污染和饵料短缺等严重影响,厦门市已采取的保护政策响应包括就地保护、迁地保护和公共管理等方面,目前种群资源数量稳定在80头左右并有潜力扩大。基于评价结果,作者提出了加强保护的建议和策略,可为厦门以外海区的中华白海豚保护工作提供参考。     

基于潜标测量的海洋环境噪声谱特性分析

利用海洋环境噪声测量潜标系统对南海典型海域开展了为期3个月的海洋环境噪声测量,16通道海洋环境噪声测量系统每小时测量两分钟噪声信号。数据处理结果表明,800~5 000Hz范围内,噪声谱与风速相关性最好,且风速越大相关性越好,噪声谱与风速的相关性好于与浪高的相关性。风关噪声谱级在海水中部基本不随接收深度发生变化,但由于测量水听器阵长度未能覆盖整个水深,因此未给出海面和海底处谱级变化规律。在400Hz以上的高频段整个风速范围内噪声谱级都随风速发生变化,且噪声谱级与对数风速具有很好的线性关系。