牙鲆dmrt1 基因的克隆及其与P450arom 基因的组织表达分析

通过基因组步移和3’RACE获得了牙鲆(Paralichthys olivaceus)dmrt1的cDNA全序列,该基因开放阅读框全长909bp,其编码的蛋白具有高度保守的DM结构域,5’UTR区含有性别相关转录因子Sox9和Sox5的结合位点。牙鲆dmrt1基因只在牙鲆的性腺中表达,且在精巢中的表达明显高于卵巢,表明牙鲆的dmrt1可能是一种性别相关基因。同时,对牙鲆的另一性别相关基因P450arom在成体各组织的表达分析结果表明,该基因除在牙鲆的性腺中有表达外,在肾脏、脾脏、鳃和脑等其他组织中也有不同程度的表达。牙鲆P450arom基因在性腺中的表达也存在两性差异,其表达模式与dmrt1的正好相反,在卵巢中的表达量明显高于精巢。     

基于三维地图视觉变量理论的三维符号设计

本文首先介绍了三维符号的一种分类方法,然后结合三维态势感知的特点,将三维地图符号视觉变量理论合理的运用到三维符号的设计中去,并从实际应用的角度分析了三维态势表达中符号设计的合理性。     

多源地理空间数据差异的成因探讨

随着地理信息的应用不断深入,人们对地理信息的需求也在不断变化,不可避免产生不同来源、格式、比例尺、多投影方式或大地坐标系统的地理空间数据.从空间实体或现象本身不确定性、人类认知表达能力的局限性、空间实体或现象观测的误差、计算机表达地理对象的局限性和空间数据操作产生的误差等方面详细分析了多源地理空间数据产生的客观和主观原因,这对GIS部门之间的数据共享和数据集成来说,具有重要应用价值.     

SPOT5影像目视判读在土地利用类型更新中的应用研究

近年来,SPOT 5影像被广泛应用于土地利用类型的调查中[1],一方面,为我们进行土地利用类型分类更新提供了新的信息源;另一方面,对遥感的室内解译提供了更高更新的要求.本文以常德市汉寿县为例探讨在进行土地利用类型分类更新中前期图像处理、解译标志的建立以及人工目视判读中的难点和重点,对于在遥感图上不易判读或容易判错的地物特征给予总结,一定程度上提高了遥感目视解译的准确性.     

cDNA cloning and expression of a collectin from red-spotted grouper （Epinephelus akaara）

Lectins play a crucial role in the innate immunity of invertebrates and vertebrates by recognizing and disposing of pathogens.We obtained the complete cDNA of a C-type lectin(EALec1) from Epinephelus akaara using RACE.The complete EALec1 cDNA sequence was 827 bp.The 5-UTR and 3-UTR were 28 bp and 151 bp,respectively,in length.The sequence also contained a polyadenylation signal AATAAA and a poly(A) tail.The EALec1 cDNA encodes polypeptides with 215 amino acids,including a signal peptide of 31 amino acids.Th...     

Development of retinal structure and visual acuity in Japanese flounder (Paralichthys olivaceus)

The retinal structure and visual acuity in Japanese flounderParalichthys olivaceus at different stages of development were examined by light microscopy. The resolving power of the retina, the visual axis and the best visual field were estimated based on the distribution of cone cells in the retina. The visual system of the larvae appears poorly developed at hatching. The larvae with total length (TL) of less than 10 mm, have single cones only and the eyes were well pigmented. At 10–11 mm TL, most single cones fused to form double cones, with the single and double cones forming a mosaic pattern. From larvae to early juvenile the retina stretches, the cones increase in diameter and rods increase in number. Based on the highest density of the cones in the ventro-temporal region, the visual axis was orientated upforward. The resolving power of the retina in 40–530 mm TL Japanese flounder was found to range from 25.1 to 11.5 min. The results indicated continual improvements in the visual system of the growing fish towards higher resolving power, visual acuity and sensitivity. Project 39970578 supported by the NSFC, and also supported by the Ministry of Education Foundation for University Key Teachers.     

实景三维在输变电工程选线规划中的应用

输变电工程选线制约因素较多,受环境影响十分明显,传统二维图选结合现场勘察的工作方式,不仅费时费力,而且无法全面掌握线路上的自然条件,导致选线效果难以保证。本文利用实景三维对输变电工程的选线规划进行了研究;在真实地理场景中进行选线范围约束、线路标绘及实景模拟,并结合空间分析技术辅助方案比选,为科学合理选线提供了一种新的思路。实践证明,在黄埠岭500 kV输变电工程建设项目中,实景三维以其真实、立体、直观的表达优势,不仅可以提高选线效率和质量,还可以在穿越风景区等特殊线路段上开展景观视觉影响分析,为方案比选与论证提供科学客观的决策参考,是对输电线路数字化选线的一次深度赋能。     

Molecular cloning and expression analysis of Crustin-like gene from Chinese shrimp Fenneropenaeus chinensis

A new member of antimicrobial protein genes of the Crustin family was cloned from haemocytes of the Chinese shrimp Fennero- penaeus chinensis by 3 ′and 5′ RACE. The full-length cDNA of Crustin-like gene contains a 390 bp open reading frame, encoding 130 amino acids. The deduced peptide contains a putative signal peptide of 17 amino acids and mature peptide of 113 amino acids. The molecular mass of the deduced mature peptide is 12. 3 ku. It is highly cationic with a theoretical isoelectric point of 8.5. The deduced amino acids sequence of this Crustin showed high homology with those of Penaeus （ Litopenaeus ） setferus. Northern blotting showed that the cloned Crustin gene was mainly expressed in haemocytes, gill, intestine, and RNA in situ hybridization indicated that the Crustin gene was constitutively expressed exclusively in haemocytes of these tissues. Capillary elec- trophoresis RT-PCR analysis showed that Crustin was up-regulated dramatically from 12 to 48 h after a brief decrease of mRNA during first 6 h in response to microbe infection. The level of Crustin mRNA began to restore at 72 h post-challenge. This indicated that Crustin gene might play an important role when shrimps are infected by bacterial pathogen.     

Efficient expression of green fluorescent protein （GFP） mediated by a chimeric promoter in Chlamydomonas reinhardtii

To improve the expression efficiency of exogenous genes in Chlamydomonas reinhardtii, a high efficient expression vector was constructed. Green fluorescent protein （GFP） was expressed in C. reinhardtii under the control of promoters： RBCS2 and HSP70A-RBCS2. Efficiency of transformation and expression were compared between two transgenic algae： RBCS2 mediated strain Tran-Ⅰ and HSP70A-RBCS2 mediated strain Tran-Ⅱ. Results show that HSP70A-RBCS2 could improve greatly the transformation efficiency by approximately eightfold of RBCS2, and the expression efficiency of GFP in Tran-Ⅱ was at least double of that in Tran-Ⅰ. In addition, a threefold increase of GFP in Tran-Ⅱ was induced by heat shock at 40℃. All of the results demonstrated that HSP70A-RBCS2 was more efficient than RBCS2 in expressing exogenous gene in C. reinhardtii.     

Characterization of defensin gene from abalone <Emphasis Type="Italic">Haliotis discus hannai</Emphasis> and its deduced protein

Defensin is one of preserved ancient host defensive materials formed in biological evolution. As a regulator and effector molecule, it is very important in animals＇ acquired immune system. This paper reports the defensin gene from the mixed liver and kidney cDNA library of abalone Haliotis discus hannai Ino. Sequence analysis shows that the gene sequence of full-length cDNA encodes 42 mature peptides （including six Cys）, molecular weight of 4 323 Da, and pI of 8.02. Amino acid sequence homology analysis shows that the peptides are highly similar （70% in common） to other insects defensin. Because of a typical insect-defensin structural character of mature peptide in the secondary structure, the polypeptide named Haliotis discus defensin （hd-def）, a novel of antimicrobial peptides, belongs to insects defensin subfamily. The RT-PCR result of Haliotis discus defensin shows that the gene can be expressed only in the hepatopancreas by Gram-negative and positive bacteria stimulation, which is ascribed to inducible expression. Therefore, it is revealed that the Haliotis discus defensin gene expression was related to the antibacterial infection of Haliotis discus hannai Ino.