Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China （LCDV-cn） genome

An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene. Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309)     

Evaluation on tourism ecological security in nature heritage sites —Case of Kanas nature reserve of Xinjiang, China

The nature heritages are the precious legacy of nature with outstanding scientific and aesthetic value. They are quite different from other common ecotourism areas, because of its original and unique system, sensitive and vulnerable landscape, and peripheral cultural features. Therefore, the tourism development in the nature heritage sites should be on the premise of ecological security. The evaluation index system of tourism ecological security in nature heritage sites was constructed in this article by AHP and Delphi methods, including nature ecological security, landscape visual security and local culture ecological security, and the security thresholds of indices were also established. In the indices’ weights of the evaluation model, the nature ecological security ranked the highest, followed by tourist landscape visual security and culture ecological security, which reflected the influence degree of the limited factor to tourism ecological security. Then, this paper carried out an empirical study of Kanas of Xinjiang Uygur Autonomous Region, China, which has the potential to be the World Nature Heritage. On the basis of the data attained from survey and observation on the spot, as well as questionnaire answered by tourists and local communities, the ecological security status in Kanas was evaluated. The result showed that the status of Kanas tourism ecological security was better, but there had some limiting factors. Lastly, effective measures were put forward to ensure its ecological security. Foundation item: Under the auspices of National Natural Science Foundation of China (No. 40671057), Knowledge Innovation Programs of Chinese Academy of Sciences (No. KZCX3-SW-355)     

整合多源异构的城市规划数据进行认知交流的研究

针对整个城市规划过程的参与者对规划数据的不同种类、不同层次认知需求,本文利用本体思想对包括GIS数据、规划文档、规划图件等多源异构的规划数据进行关系重构,设计本体模型要素的数据语义关系,定义数据间的关联关系,实现基于本体方法的规划领域语义表达算法,方便了各类规划参与者对规划方案认识的交流:同时根据用户特征分类,进行本体关系的逻辑推理,过滤冗余信息,进行多源异构规划数据的可视化。     

基于交通轨迹数据的三维动态噪声地图

噪声信息具有空间传播和随距离衰减变化的特征,采用三维动态可视化形式表达噪声地图可以体现该特征。本文针对噪声的空间分布与强度变化提出一种噪声地图表达的方法,基于Bertin符号参量分析,以具有高度可变的方柱并配以深浅变化的颜色作为噪声符号,动态传递噪声信息。为进行多尺度地图展示,在不同地图等级下设有相应细节层次的符号尺寸和定位排列方式。噪声地图以Web端Mapbox为展示平台,基于交通噪声模型和GPS轨迹数据,对上海黄浦区某区域一时间段内的噪声情况进行模拟表达,实现噪声数据的立体动态化显示。三维动态的表达扩展了噪声数据的表现维度,多维度、多参量的立体动态符号不仅可准确刻画噪声的分布,而且从视觉观感上提高了受众的兴趣度和专注度,这为研究噪声的热点分布、传递趋势等空间规律提供了可视化参考。     

雄性半滑舌鳎雄激素受体基因的克隆与表达分析

利用RT-PCR方法从半滑舌鳎(Cynoglossus semilaevis)中克隆了雄激素受体(Androgen receptor,AR)基因cDNA的部分序列(434 bp),经BLAST比对与狼鲈(Dicentrarchus labrax)、花溪(Kryptolebias marmoratus)、三斑海猪鱼(Halichoeres trimaculatus)和三刺鱼(Gasterosteus aculeatus)的雄激素受体同源性分别为84%,82%,82%和81%.组织表达分析表明,AR基因在半滑舌鳎的性腺、肝、胃、脾、肾、头肾、肠、鳃、心、脑和肌肉这11种组织中均有表达,表达量有所差异.肾中表达量最丰富,鳃中最少.根据其他硬骨鱼类表达模式,推测半滑舌鳎可能只含有1种AR.     

融合光度参数估计的单目直接法视觉里程计

针对单目直接法视觉里程计在光照变化大的场景下定位精度低、定位鲁棒性差的问题,文中提出一种融合光度参数估计的单目直接法视觉里程计.首先通过对图像帧进行特征匹配实现特征跟踪,建立起图像像素点之间的关联,接着利用非线性优化方法进行光度参数估计,然后在光度参数估计提供的光度参数先验信息的基础上对图像灰度值进行校正,最后利用校正...     

Direct observation of complex Tóthian groundwater flow systems in the laboratory

Based on the theory of gravity‐driven groundwater flow systems, we have developed a complex Flow System Sand‐Box Model (FSM). It enables the visual observations of the development and characteristics and temporal evolution of complex Tóthian flow systems in the laboratory. The configuration of the regional, intermediate and local flow systems can be controlled and observed; hydraulic head, flow direction and travel time can be measured; and the scale and shape of the sub‐flow systems as well as the path lines and flow lines can be observed directly. The experiments demonstrate the Tóthian flow systems in a small basin with multiple sources and sinks. Greater local topographic (water table) undulation will lead to larger local flow systems. Greater regional and less local topographic undulation will enhance the development of intermediate and regional flow systems. In homogeneous media, increasing fluid‐potential differences between source and sink increase the spatial scale of the generated flow systems. The FSM is a useful teaching aid and experimental device to study and develop an intuitive insight into gravity‐driven groundwater flow systems. It helps to visualize and understand the hydraulic properties and controlling factors of Tóthian flow systems and may be used to study problems related to the chemical and temperature characteristics of the flow systems as well. Copyright © 2010 John Wiley & Sons, Ltd.     

Areal extent of the 1988 flood in Bangladesh: How much did the satellite imagery show?

This paper presents the methods and results of visual interpretation of satellite imagery for estimating areal extent of the 1988 flood in Bangladesh. The main limitation of this method was that cloud-free images were not available for the peak flood period in the first week of September. Relatively cloud-free images on three selected dates in the last three weeks of September showed that areas of inundation on those dates ranged from 44000 to 60000 km² ± 5% (31 to 42% of Bangladesh). The Government of Bangladesh official estimates of the areal extent of flooding were tentative in nature, since these were based on a choropleth map of relative proportions (percentages) of flooding in different parts of the country. The official estimate of the maximum extent of flooding was 82000 km² (57% of Bangladesh), i.e. 15 to 26% larger than the area shown on satellite imagery. The actual extent of flooding was certainly larger than the estimates from satellite imagery but, perhaps, smaller than the official estimates, since a reduction of 15 to 26% of flood area in one to three weeks seemed unlikely. An alternative method of mapping flood-affected areas by using newspaper-interpreted data was attempted, but the method had limited values because of reporting bias.     

视景地形仿真及其应用

本文主要讨论了视景地形仿真的特点、数据基础及其实现的整个过程，列举了视景地形仿真在军事上和其它领域的应用实例。实验表明，本文所介绍的视景地形仿真过程简单而容易实现，仿真的地形景观具有良好的视觉效果。     

微机双道原子荧光光度计测控系统设计

该系统采用串口通信方式完成嵌入式单片机系统^[1]和计算机之间通信，通过测量待测溶液和标样的荧光强度来计算待测液中某痕量元素的含量，该系统具有图形化友好菜单操作、可视化的曲线分析和数据处理等特点。