土壤重金属生物有效性研究进展

土壤重金属污染具有巨大环境风险。笔者围绕土壤重金属元素生物有效性(可给性)概念、元素形态与有效性影响因素、有效态(可给态)实验技术,综述了国内外研究现状。土壤金属元素形态和生物有效性取决于其地球化学行为、元素成因来源、土壤理化条件(p H值、有机质、粘土矿物与化学活性矿物、土壤粒级组成等)以及植物根际效应等。选择性单步提取和连续提取是检测土壤元素形态、有效态的有效和可行方法。针对手—口是土壤铅等污染物在儿童群体暴露的重要途径,发展了生物可给态体外试验方法。土壤污染物生物有效性和可给性已成为土壤污染风险评价的重要指标参数,实验成果也为土壤污染修复提供了重要依据。     

柴达木盆地东部石炭系古岩溶缝洞单元测井响应

柴达木盆地石炭系多发育致密化储层,后期成岩改造尤为重要。目前在盆地东部多口钻井中发现石炭系存在孔洞、溶缝以及溶蚀残丘等不同尺度的岩溶改造迹象,且个别钻井在该段有明显气测异常。因此,岩溶缝洞特征、缝洞测井响应是该区石炭系储层的基础问题。鉴于此,以柴东钻井资料为基础,岩石学-测井地球物理方法相结合,开展古岩溶缝洞单元测井响应研究。研究表明,石炭系岩溶缝洞可划分为风化壳型岩溶和沿构造断裂有关的岩溶缝洞两种类型。岩溶多见于克鲁克1~2段、扎布萨尕秀组以及怀头他拉与上覆层界限处。缝洞横向可对比,往往自然电位负异常,自然伽马中低值（20.5~35.0 API）,声波时差异常增高,中子（13.9%）、密度（2.50 g/cm3）有一定镜像特征,电阻率（215.2~1100.0 Ω·m）低于围岩（>1100.0 Ω·m）。该区成熟的风化壳型岩溶缝洞在测井上表现为五个结构单元,上覆层、渣状层、溶洞层、裂隙-溶缝层、致密层。岩溶风化壳缝洞为该区主要岩溶缝洞类型,改造强度大于裂缝型,且共生。二者改造机制各异,但改造时间近似,推测为印支期的构造响应,时间持续到早中侏罗世甚至到早白垩世结束。      

活动态测量中滤材的选择及其应用

研究滤材对元素活动态测定结果及测定精密度的影响,综合考虑元素活动态测量结果的真实性以及土壤胶体的性质等因素,确定了2μm孔径的滤膜是用于活动态测量的较佳滤材,本研究对活动态测量方法的规范化、标准化具有重要意义。应用试验表明所选滤材适用于活动态测量。     

过套管电阻率测井仪器的径向探测特性

为研究过套管电阻率测井仪器的径向探测特性,本文给出了反映地层径向介质变化的几何因子.利用过套管电阻率测井的传输线方程实现了电阻率测井几何因子的数值计算,针对Schlumberger公司的CHFR测井仪和俄罗斯的ЭКОС-31-7测井仪分别计算了几何因子响应曲线,通过曲线分析发现：两种测井仪对低侵剖面（有低阻流体侵入）均有较大的探测半径,但随侵入流体电阻的增大（高侵剖面）仪器的径向探测半径在减小,本文的研究为地层电阻率测井解释和仪器径向探测特性分析提供了理论依据.     

米槠天然林土壤真菌对N2O产生的贡献

研究表明亚热带地区森林土壤硝化和反硝化能力都非常低,但却是全球N2O最大的自然源之一,因而推测极有可能存在N2O潜在源未被认识,有迹象表明真菌最有可能是这一潜在源的贡献者,但实际研究证据并不多．为此本研究以中亚热带地区典型常绿阔叶林——武夷山自然保护区米槠天然林土壤为对象,利用室内基质诱导呼吸选择抑制方法,研究在水分60％WHC和温度25℃条件下土壤真菌细菌活性比及其对N2O产生的相对贡献．结果显示真菌和细菌的活性比例分别是0．7±0．3和0．3±0．1,前者显著大于后者（P〈0．05）,真菌与细菌的活性比为2．00±1．00;与对照相比,添加放线菌酮（真菌抑制剂）处理N2O产生速率减少了50．8±11．3％,添加链霉素（细菌抑制剂）处理则减少了43．7±11．8％,前者减少是后者的1．15倍,但差异不显著．研究结果表明该米槠天然林土壤在给定实验条件下真菌活性比细菌的大,但二者对土壤N2O产生的贡献几乎相等．基质诱导呼吸选择抑制方法具有较多的限制条件,若得出可靠结论,还应该利用包括生物化学、分子生物学方法在内的多种方法进行相互验证．同时今后应加强真菌产生N2O途径及机理研究．     

基于小波变换图像分割技术的电成像测井资料裂缝、孔洞面孔率提取方法

对于裂缝、溶蚀孔洞发育的碳酸盐岩缝洞储层,如何从测井资料中提取裂缝、溶蚀孔洞信息是评价储层有效性的关键问题.为了从电成像测井静态图像上准确地分割出清晰的裂缝、溶蚀孔洞子图像进而提取其参数信息,本文提出了一种基于小波变换模极大值图像分割技术的电成像测井资料缝洞面孔率提取方法.以钮扣电极电导率曲线为对象,先消除井壁凹凸不平导致的地层背景噪声的影响,利用小波变换模极大值图像分割方法得到包含裂缝和溶蚀孔洞目标的子图像,根据子图像提取裂缝-孔洞总面孔率、裂缝面孔率、孔洞面孔率等信息.本文利用塔里木盆地奥陶系碳酸盐岩地层的电成像测井数据提取了缝洞面孔率参数,还利用同井岩心CT扫描图像计算的平均缝洞面孔率、双侧向电阻率、常规测井资料三孔隙度模型计算的相对连通缝洞孔隙度进行了对比,并进行了试油验证.对比表明,电成像测井裂缝-孔洞总面孔率、裂缝面孔率、孔洞面孔率与岩心CT扫描图像平均缝洞面孔率、双侧向电阻率、相对连通缝洞孔隙度、试油结果均有较好的一致性.这一方面验证了采用本文方法提取的裂缝-孔洞总面孔率、裂缝面孔率、孔洞面孔率的合理性,另一方面表明所提取参数可用于指示缝洞型碳酸盐岩储层的渗透性和有效性.

     

Similar morphological and chemical variations of Gloeocapsomorpha prisca in Ordovician sediments and cultured Botryococcus braunii as a response to changes in salinity

Most Ordovician source rocks consist of accumulation of a colonial marine microorganism, Gloeocapsomorpha prisca (G. prisca) whose nature, ecology and affinity with extant organisms have been in dispute for years. Furthermore, recent studies have shown major differences in phenol moieties between two G. prisca-rich samples. Examination of five G. prisca-rich kerogens by electron microscopy and pyrolysis studies revealed (i) the occurrence of two markedly distinct “morpho/chemical” types: a “closed/phenol-rich” type (Baltic samples) and an “open/phenol-poor” one (North American samples) and (ii) the selective preservation of the resistant micromolecular material building up the thick cell walls in the original organism. Comparison with extant Botryococcus braunii (a widespread green microalga) grown on media of increasing salinity suggests that G. prisca is likely to be a planktonic green microalga related to B. braunii, which can adapt to large salinity variations which, in turn, control its polymorphism. The large differences in colony morphology and in the content of phenol moieties observed in fossil G. prisca and the resulting occurrence of two “morpho/chemical” types, should therefore reflect depositional environments with different salinities. The presence of thick, highly aliphatic, resistant walls in G. prisca selectively preserved during fossilization, accounts for the major contribution of this organism to Ordovician organic-rich sediments and for the resulting typical signature of Ordovician oils.     

Induced Calcite Precipitation by Cyanobacterium Synechococcus

This study investigated the role of the solution composition on calcite precipitation induced by cyanobacteria. The precipitation of calcium carbonate was induced by addition of cyanobacterium cells Synechococcus strain PCC 7942 in two artificial solutions with a different composition at similar saturation states in respect to calcite. Ion‐selective electrodes for pH, Ca²⁺, and COequation/tex2gif-stack-1.gif monitored the experiments, and the morphology of precipitated crystals was analysed by scanning electron microscopy. The calcite precipitation was observed in all experiments after the addition of the cells. The composition of solution (the ratio of dissolved inorganic carbon to dissolved calcium) strongly influenced the calcite precipitation. Based on laboratory experiment results, a possible mechanism for precipitation induced by Synechococcus is proposed linking precipitation with the conditions near to cell walls rather than with the saturation conditions in the bulk solution.     

东营凹陷大芦湖地区沙三段油藏研究

针对大芦湖地区沙三中、下砂体上覆高速火成岩体与巨厚高青砂岩体的地质特征，利用大芦湖地区测井、钻井、地质等资料相结合，寻找高青大砂体的分布规律，形成了一套解释樊家砂体成因的理论：块体滑塌理论。并且利用该区的声波测井及速度谱资料，分析该区特殊地质体、储层、围岩层速度及平均速度变化，采取相应措施提高沙三中、下砂体识别能力并准确地进行砂体等时图时深转换。在测井约束反演时，为了消除目的层上部的高速地质岩体的屏蔽作用，对测井声波作了预处理，相应地提高了目的层的速度，从而突出目的反射层阻抗界面。使用预处理的声波，进行多井约束反演，目的层清晰，基本消除了上覆高速地质岩体的屏蔽作用，因而有效地提高了目的层砂三中、下砂体的识别能力。     

Petrophysical characterization and natural fracturing in an olivine-dolerite aquifer

As part of a study investigating the naturally-occurring fractures in mafic rocks, two holes were drilled 450 m apart through the Palisades dolerite sill in New York. Well-2 is 229 m deep and Well-3 was drilled to 305 m, both penetrating through the sill and into the underlying Triassic sediments of the Newark Basin. Both holes were logged with downhole geophysical tools, including the BHTV, which acoustically images fractures intersecting the well. Understanding the fracture pattern, density, and porosity in the sill is essential for identifying possible zones of active fluid flow and high permeability. Using the BHTV logs, 96 and 203 fractures were digitally mapped within the sill in Well-2 and Well-3, respectively. Most fractures appear to dip steeply (76-78°). There is a shift in fracture orientation, however, and these fractures may or may not be continuous over the short lateral distance between Well-2 and Well-3. The lithology of the sill as identified by drill chips is nevertheless continuous between the holes. Both intersect a 7 m thick olivine-rich layer about 15 m above the bottom of the sill. Several fractures identified in Well-2 have large apparent aperture (>6cm) which correspond to high porosity zones (6-14%) observed in the logs. Resistivity logs were used to compute porosity using Archie's law and match well with the neutron porosity log in Well-2. We use the relationship between porosity and fracture aperture within the sill at Well-2 to infer the porosity in Well-3. High-porosity, large-aperture zones, including the target olivine layer, are identified in both holes. Changes in the temperature gradient log indicate active fluid flow in the sill, although flow appears to be most active in the sediments. Direct field measurements of bulk permeability, hydrologic modeling of fluid flow and calibration of fracture and log porosity will be undertaken in the future.