6-磷酸山梨醇脱氢酶(gutD)基因克隆、测序和表达

本研究根据6-磷酸山梨醇脱氢酶基因(gutD)两端序列设计引物，以假单胞杆菌(Pseudonmonas XM)的总DNA为模板通过PCR的方法克隆gutD基因并进行序列分析，将克隆得到的gutD基因与原核表达载体pBV220连接并转化大肠杆菌(Escherichia coli)JM101后检测其蛋白表达及菌株生长耐盐性。     

地层学的发展推动油气地质理论研究的进步

自上个世纪50年代以来,地层学经历了彻底的变革,逐渐由“相模式”、“沉积体系”、“地震地层学”发展到目前的“层序地层学”,形成高分辨率层序地层学和事件地层学的理论体系和研究方法。文章围绕层序地层学、高分辨率层序地层学基本方法及理论体系,与含油气系统相关关系进行了讨论。     

An Application of Direct Ritz Method for Random Seismic Responses of Jacket Platforms

1 .IntroductionNowadays tremendous efforts have been devotedtothe analysis of randomseismic responses .Forexample ,American PetroleumInstitute (API) has declared that the analysis of randomseismic re-sponsesis a useful tool for safety inspection. Owing to the complexity of platformand randomness ofload,it is difficult to carry out the randomresponse analysis of a jacket platform. The conventionalrandomvibration methods ,such as square root of the sumof squares (SRSS) and complete quadratic…     

湍流数值模拟中封闭模式应用的局限性

通过对琼州海峡的潮流场特征进行数值模拟，指出了选择不同的特征混合长度表达式对数值模拟结果的影响，表明了基于特征混合长度理论的流封闭模式在近海湍流数值模拟中应用的局限性。     

1994年发生在台湾海峡的一次地震海啸的数值模拟

建立了一个地震海啸数值模式,模式包含越洋海啸传播部分和近岸海啸变形部分,在越洋海啸传播部分中采用线性浅水方程,使用蛙跃格式求解,并且选择合适的空间步长与时间步长,使差分格式中产生的数值频散与包辛尼斯克方程中的物理频散一致,这样在不影响海啸数值计算精度的前提下,节省了计算机的机时与内存.在近岸海啸变形部分的计算中,考虑了非线性对流项与海底摩擦项.同时该模式采用了多重网格嵌套技术,提高了所关心地区的计算精度.利用这个地震海啸模式模拟了1994年发生在台湾海峡的一次地震海啸,结果与观测记录较吻合.这个模型已用于我国沿海核电站可能最大地震海啸的数值计算.     

Research on Maneuverability and Simulation of Underwater Vehicles for Pipeline Detection and Maintenance

1 .IntroductionWiththe advent of underwater vehicle technology in the offshore industry, major survey and in-spectiontasks can be done at lower cost withfaster execution.Pipe inspection is of major interest inthat respect because thousands of meters of pi…     

塘沽海区海底地形的SAR影像仿真与反演研究

利用袁业立、金梅兵(1997)提出的海底地形SAR影像仿真与反演模型，对渤海塘沽海区的一张Radarsat SAR影像进行了仿真和水深反演研究。研究结果表明：仿真影像与真实SAR影像基本吻合，反演水深与实际水深也有较好的一致性；进一步证实了袁业立(1997)SAR成像机理的正确性和在中国近海利用SAR影像进行浅海水深探测的可行性。     

Dynamics and control of a towed underwater vehicle system, part I: model development

Autonomous vehicles are being developed to replace the conventional, manned surface vehicles that tow mine hunting towed platforms. While a wide body of work exists that describes numerical models of towed systems, they usually include relatively simple models of the towed bodies and neglect the dynamics of the towing vehicle. For systems in which the mass of the towing vehicle is comparable to that of the towed vehicle, it becomes important to consider the dynamics of both vehicles. In this work, we describe the development of a numerical model that accurately captures the dynamics of these new mine hunting systems. We use a lumped mass approximation for the towcable and couple this model to non-linear numerical models of an autonomous surface vehicle and an actively controlled towfish. Within the dynamics models of the two vehicles, we include non-linear controllers to allow accurate maneuvering of the towed system.     

A Method of Coding and Decoding in Underwater Image Transmission

A new method of coding and decoding in the system of underwater image transmission is introduced, including the rapid digital frequency synthesizer in multiple frequency shift keying,image data generator, image grayscale decoder with intelligent fuzzy algorithm, image restoration and display on microcomputer.     

Hydrocarbon Potential of Pre-cenozoic Strata in the North Yellow Sea Basin

The North Yellow Sea Basin ( NYSB ), which was developed on the basement of North China (Huabei) continental block, is a typical continental Mesozoic Cenozoic sedimentary basin in the sea area. Its Mesozoic basin is a residual basin, below which there is probably a larger Paleozoic sedimentary basin. The North Yellow Sea Basin comprises four sags and three uplifts. Of them, the eastern sag is a Mesozoic Cenozoic sedimentary sag in NYSB and has the biggest sediment thickness; the current Korean drilling wells are concentrated in the eastern sag. This sag is comparatively rich in oil and gas resources and thus has a relatively good petroleum prospect in the sea. The central sag has also accommodated thick Mesozoic-Cenozoic sediments. The latest research results show that there are three series of hydrocarbon source rocks in the North Yellow Sea Basin, namely, black shales of the Paleogene, Jurassic and Cretaceous. The principal hydrocarbon source rocks in NYSB are the Mesozoic black shale. According to the drilling data of Korea, the black shales of the Paleogene, Jurassic and Cretaceous have all come up to the standards of good and mature source rocks. The NYSB owns an intact system of oil generation, reservoir and capping rocks that can help hydrocarbon to form in the basin and thus it has the great potential of oil and gas. The vertical distribution of the hydrocarbon resources is mainly considered to be in the Cretaceous and then in the Jurassic.