区域资源定量评价中面金属量法的应用

地球化学方法被普遍应用于矿产资源评价,但多数方法都只是基于地球化学数据本身而进行的,较少考虑除成矿物质来源之外的诸如构造、地层等地质环境的影响。文章运用面金属量法,在充分研究地质因素的基础上,确定了预测单元及地球化学背景值,在一定程度上屏蔽了虚假资源量,对东天山地区铜的资源量进行了定量评价。预测认为,东天山地区铜的资源潜力巨大。     

Quantitative relationship between pollen and vegetation in northern China

205 surface pollen samples from different communities in Northern China were analyzed to understand the quantitative relationship between pollen and its original vegetation. Pollen analysis and vegetation investigation show that the pollen assemblages differ a lot in different vegetation regions. Arboreal pollen account for more than 30% in temperate broad-deciduous forests region. In temperate steppe regions, herb pollen percentages are more than 90%, where Artemisia and Chenopodiaceae are domi- nant pollen types with Artemisia percentages more than 30%. In temperate desert, Chenopodiaceae pollen percentages are more than Artemisia, where ferns are rare. Cyperaceae pollen percentages are more than 20% in sub-alpine or cold meadows. The relations between pollen percentages and vegeta- tion cover indicate that most arboreal pollen shows a close relationship with parent plant covers, most shrubby pollen types have more or less correlations, but most herbs do not show clear correlations. For arboreal pollen types, Picea pollen shows the closest correlation with spruce trees coverage, then is Quercus and Carpinus. Betula, Larix and Juglans have also high correlation coefficients with their plants coverage, but Betula pollen is of overrepresented pollen type and more than 40% in birch forest, while Larix and Juglans pollen is underrepresented and pollen percentages are more than 10% in Larix or Juglans pure forests. Pinus is of overrepresented pollen type, and pollen percentages have some relations with plants cover. Pine forest might present when Pinus pollen percentages are more than 30%. The relations between Ulmus and Populus pollen percentages and vegetation cover are not close, where they are mixed with other arbors, they cannot be recorded easily, but if their pollen percentages are more than 1%, Ulmus or Populus trees should exist. For shrubby pollen types, the correlation be- tween Vitex pollen percentages and vegetation cover is the highest, then is Corylus, Tamariaceae and Nitraria, and their pollen percentages are less than 1% where original plant are absent. Caragana and Spiraea pollen percentages have some relations with vegetation cover. The relations between pollen percentages and vegetation cover are not clear for Rosaceae and Saxifragaceae. For herb pollen types, Cyperaceae pollen has the closest correlation with vegetation cover, where pollen percentages are more than 20% when Cyperaceae are constructive or dominant species in vegetation, and pollen per- centages are less than 5% where Cyperaceae are not constructive or dominant species (cover less than 30%). Artemisia and Chenopodiaceae pollen percentages mainly have close relations with ecological regions. The relations between pollen percentages and cover are not clear for Gramineae, Legumi- nosae and Compositae.     

湖北省耕地资源利用效益研究

构造了耕地产出效益系数、耕地消耗回报系数、耕地污染替代系数和耕地利用集约化系数等指标，对湖北省现有耕地资源的利用效益进行了定量分析。在此基础上，从观念更新、加大财政投资力度、优化农地使用制度等方面，提出了提高湖北省耕地资源利用效益的宏观对策，为合理利用耕地资源提供了建议。     

武汉台形变观测与降雨参数之间的定量分析与研究

基于“前兆台网(站)观测数据跟踪分析平台”,对武汉台形变观测资料进行了系统分析,提取出观测曲线受降雨干扰影响的事件,采用降雨总量、初始驱动降雨量和瞬时降雨量最大值等降雨参数对降雨干扰事件进行统计分析。结果表明:降雨总量达40 mm、初始驱动降雨量为0.3 mm或瞬时降雨量最大值达0.6 mm时,DSQ型水管倾斜仪易受降雨干扰;SSY型铟瓦棒伸缩仪当降雨总量超60 mm或瞬时降雨量最大值大于0.5 mm时易受降雨干扰;VS型垂直摆倾斜仪受降雨干扰与降雨总量、初始驱动降雨量和瞬时降雨量最大值无显著相关关系;降雨总量对形变仪器观测物理量的影响基本呈现线性;而形变仪器观测物理量与初始驱动降雨量、瞬时降雨量最大值无显著相关关系。认为武汉台形变观测受降雨影响主要来自降雨渗透影响和周边水体荷载变化影响两个方面。     

应用实时定量PCR技术检测秦皇岛近岸海域褐潮原因种——抑食金球藻(Aureococcus anophagefferens)

Aureococcus anophagefferens, a small pelagophyte algae, has caused brown tide blooms in coastal waters of Qinhuangdao in recent years, presenting significant negative impacts on the shellfish mariculture industry. Under standard light microscopy, it is visually indistinguishable from other small algae in field samples due to its extremely small size. In this study, quantitative polymerase chain reaction(q PCR) based on 18 S r DNA sequences was developed and used to detect and enumerate A. anophagefferens. A linear regression(R2 = 0.91) was generated based on cycle thresholds value(Ct) versus known concentrations of A. anophagefferens. Twenty-two field samples collected in coastal waters of Qinhuangdao were subjected to DNA extraction and then analyzed using q PCR. Results showed that A. anophagefferens had a wide distribution in coastal waters along Qinhuangdao. Elevated A. anophagefferens abundance, category 3 brown tide blooms(200 000 cells/m L) occurred at Dongshan Beach and Tiger-stone Beach in August in 2013. In shellfish mariculture areas along coastal waters of Qinhuangdao, 4 stations had category 3 blooms, and 6 stations had category 2 blooms(35 000–200 000 cells/m L) in August and all stations had category 1 blooms(0 to ≤35 000 cells/m L) in October. Quantitative PCR allows for detection of A. anophagefferens cells at low levels in filed samples, which is essential to effective management and prediction of brown tide blooms.     

Evaluation of radar and automatic weather station data assimilation for a heavy rainfall event in southern China

To improve the accuracy of short-term(0–12 h) forecasts of severe weather in southern China, a real-time storm-scale forecasting system, the Hourly Assimilation and Prediction System(HAPS), has been implemented in Shenzhen, China. The forecasting system is characterized by combining the Advanced Research Weather Research and Forecasting(WRF-ARW)model and the Advanced Regional Prediction System(ARPS) three-dimensional variational data assimilation(3DVAR) package. It is capable of assimilating radar reflectivity and radial velocity data from multiple Doppler radars as well as surface automatic weather station(AWS) data. Experiments are designed to evaluate the impacts of data assimilation on quantitative precipitation forecasting(QPF) by studying a heavy rainfall event in southern China. The forecasts from these experiments are verified against radar, surface, and precipitation observations. Comparison of echo structure and accumulated precipitation suggests that radar data assimilation is useful in improving the short-term forecast by capturing the location and orientation of the band of accumulated rainfall. The assimilation of radar data improves the short-term precipitation forecast skill by up to9 hours by producing more convection. The slight but generally positive impact that surface AWS data has on the forecast of near-surface variables can last up to 6–9 hours. The assimilation of AWS observations alone has some benefit for improving the Fractions Skill Score(FSS) and bias scores; when radar data are assimilated, the additional AWS data may increase the degree of rainfall overprediction.     

马氏珠母贝(Pinctada fucata)组织蛋白酶L基因的克隆与表达分析

根据已构建的溶藻弧菌(Vibro alginolyticus)诱导的马氏珠母贝(Pinctada fucata)血淋巴cDNA差减文库得到的ESTs序列, 应用cDNA末端快速扩增(RACE)技术成功克隆了其组织蛋白酶L基因(PFCatL), 并对其进行了生物信息学分析; 应用实时荧光定量PCR (Real-time PCR)技术, 研究了PFCatL基因在溶藻弧菌刺激前后马氏珠母贝足、外套膜、鳃、闭壳肌等8个组织中的表达变化。结果表明, PFCatL基因cDNA全长2004bp, 其中5′非编码区(5′-UTR)50bp, 3′非编码区(3′-UTR)865bp, 开放阅读框(ORF)1089bp, 编码362个氨基酸, 其分子量计算值(MW)为40.52kDa, 理论等电点(IP)为5.20; 生物信息学分析表明, PFCatL含有16个氨基酸残基组成的信号肽序列以及组织蛋白酶前体抑制功能域I29; Clustalw2多重比对发现PFCatL氨基酸序列在催化三联体Cys-His-Asn、底物结合位点以及二硫键形成相关的半胱氨酸残基位点高度保守; Real-time PCR研究发现, PFCatL在马氏珠母贝各组织中均有表达, 但各组织间的表达量存在差异, 其中以肾和闭壳肌中的表达量最高; 溶藻弧菌感染4h后, 外套膜、鳃以及血淋巴中PFCatL基因的表达较感染前显著上调。     

拟穴青蟹(Scylla paramamosain)蛋白二硫键异构酶基因的分子克隆与表达分析

蛋白二硫键异构酶(PDI)是内质网中的关键酶, 参与蛋白合成过程中二硫键的形成、还原和异构。本文首次从拟穴青蟹(Scylla paramamosain)克隆获得了PDI基因cDNA全长, 该序列长度为2015bp, 开放阅读框为1452bp, 编码483个氨基酸。荧光定量PCR检测发现PDI存在于拟穴青蟹的多个组织中; 在拟穴青蟹卵巢发育过程中, PDI基因的表达量在前4个时期逐渐上升, 到了第5期(成熟期)表达量则下降, 表明PDI参与了卵巢发育的蛋白合成过程。免疫组化表明, 拟穴青蟹卵母细胞存在PDI阳性反应, 进一步为该分子参与卵巢发育提供了形态学证据。     

硅酸盐玻璃中的Na和K含量电子探针分析条件设定探讨

含Ti硅酸盐玻璃在基础科学研究和应用技术开发上均有重要价值,亟需对其进行准确的成分分析,为进一步的科研工作提供重要的数据支撑.对玻璃进行电子探针分析时,由于样品的易损性和其中(Na和K)阳离子在电子束轰击下极易发生迁移和扩散,因此往往需要经过条件实验来确定合适的分析条件,确保样品在该条件下能够保持尽量稳定的状态,才能获...     

危机矿山深部、边部隐伏矿体的三维可视化预测——以安徽铜陵凤凰山矿田为例

以安徽铜陵凤凰山矿田为例,通过综合地质研究,构建了矿体定位预测概念模型。在地质勘探数据和物化探数据集成的基础上,采用三维地质建模技术,对地层、构造、岩浆岩、矿体等地质体进行了推断和圈定,构建了地质体的线框模型与块体模型。基于定位预测概念模型和地质体三维块体模型,通过地质空间定义和立体单元划分,建立了岩体及其表面形态起伏、接触带、地层、褶皱、断层等控矿地质因素的三维栅格场模型。在定量分析控矿地质因素与矿化分布的关系的基础上,建立了反应这种关联关系的矿体立体定量预测模型。预测模型提供矿田深部(-1000m标高以上)所有50m×50m×50m立体单元的铜品位、铜金属量和含矿概率的预测结果。基于预测结果圈定了4个深部找矿立体靶区,为深部找矿工程的设计、布置提供尚未发现的隐伏矿体的位置、品位、金属量等信息的指导。