Initiation of primary cell culture from amphioxus <Emphasis Type="Italic">Branchiostoma belcheri tsingtauense</Emphasis>

Amphioxus, a cephalochordate, is an important model fish for studies in evolution and comparative biology. A successful cell culture from amphioxus tissues in vitro would help understanding some basic issues. To determine the optimal culture conditions for proliferation of amphioxus cells, primary cultures were initiated from buccal cirri, tail, gill, gut and metapleural fold of amphioxus Branchiostoma belcheri tsingtauense. The media tested were L-15, F-12, M 199, MEM, DMEM, PRMI 1640 and LDF, each was supplemented with 20% fetal bovine serum. The optimal conditions include tail tissue cultured in L-15 or F-12 with supplement of 20% FBS and 1.5% NaCl at about 25°C. Supported by Doctoral Initial Fund of Ludong University (No.43304)     

Argo floats revealing bimodality of large-scale mid-depth circulation in the North Atlantic

Analysis of Argo float trajectories at 1 000 m and temperature at 950 m in the North Atlantic between November 2003 and January 2005 demonstrates the existence of two different circulation modes with fast transition between them. Each mode has a pair of cyclonic - anticyclonic gyres. The difference is the location of the cyclonic gyre. The cyclonic gyre stretches from southeast to northwest in the first mode and from the southwest to the northeast in the second mode. The observed modes strongly affect the heat and salt transport in the North Atlantic. In particular, the second mode slows down the westward transport of the warm and saline water from the Mediterranean Sea.     

深水滑翔机器人耐压壳体结构优化设计

针对深水滑翔机器人耐压壳体在深水中压缩变形和海水密度随水深增大而变化等因素造成驱动浮力变化问题,利用压缩变形和密度变化对驱动浮力影响的互补特性,归纳了载体耐压壳体结构优化设计方法.保证耐压壳体抗压条件和质量最小前提下采用结构优化设计方法,降低耐压壳体压缩变形和海水密度变化对驱动浮力的影响,提高系统能源的利用率.     

带乘性噪声广义系统状态最优估计算法

针对带乘性噪声广义系统,提出1种在线性最小方差意义下的状态最优估计算法.首先,采用标准分解将系统变换为2个子系统;其次,通过估计子系统的状态,获得原系统的状态最优滤波.同时,算法还给出了动态噪声与量测噪声的估计.仿真结果表明了该算法的有效性.     

多尺度带乘性噪声系统的最优状态平滑算法

针对多尺度带乘性噪声系统,在多尺度最优滤波融合的基础上,进行状态最优固定域平滑算法的研究.通过推广得到的平滑算法需要大量的局部传感器参数,而分布式多尺度滤波融合后不能保留这些信息.针对这一弊端对算法进行改进,推导出仅使用融合后的一步预测及滤波值的平滑算法.该算法在线性最小方差意义下是最优的.计算机仿真验证了算法的可行性.     

最优TS评分方法在逐时降水订正中的初步应用及评估

使用2020年3—9月逐时更新的CMA广东短临3 km数值模式(CMA-GD(R3)模式)1～12 h逐小时降水量资料,利用最优TS评分订正方法(OTS)对逐小时降水量进行分级订正,并分别从整体和分类型降水过程预报订正效果进行了检验和对比评估。结果表明：从整体预报订正性能来看,通过OTS方法对CMA-GD(R3)模式订正后,对于≥1 mm/h及以上量级的降水,OTS均有较好的订正能力,并且随着雨强的增加,其TS评分的改善比率越大;同时,OTS可有效减少各个预报时效的漏报率和空报率,其中漏报率减小更加明显,表现出明显的湿偏差(空报偏多)。从三类暴雨过程逐时降水预报订正效果来看,通过OTS订正之后,对于≥1 mm/h的降水,OTS对三类暴雨类型均有正的订正能力。其中在0.1 mm、1 mm、10 mm、20 mm、35 mm、50 mm 6个量级上,季风型的逐时降水预报表现最好,6个量级的TS评分值分别为0.403、0.232、0.053、0.023、0.009和0.004;在5 mm量级上锋面型的逐时降水预报表现最优,其TS值为0.102。从改善效果来看,经过OTS订正后,在1 mm量级...     

基于遗传算法的浮式防波结构物形状最优化设计

采用遗传算法作为优化手段,以透射系数为目标函数,对给定横剖面面积的浮式防波结构物在规则波中的消波性能及剖面形状进行了最优化计算研究,探讨了透射系数最小的剖面形状与波浪周期之间的关系,为浮式防波结构物的设计提供了一定的理论依据     

VTI介质qP波方程高精度有限差分算子

波动方程有限差分法是一种使用广泛的地震波数值模拟方法.但是有限差分法本身固有存在着数值频散问题,会降低地震波场模拟的精度与分辨率.为了克服常规有限差分算子的数值频散,本文针对VTI介质地震波数值模拟问题,构造了频率-空间域qP波波动方程高精度有限差分优化算子,根据最优化理论中高斯-牛顿法确定了高精度有限差分算子的优化系数.利用常规差分算子和高精度优化差分算子对归一化相速度的频散关系精度进行了对比分析,并对均匀各向同性介质和均匀VTI介质中的qP波地震波场进行了有限差分数值模拟,通过频散关系精度分析和波场数值模拟结果表明：有限差分优化算子具有较高的波场数值模拟精度,有效压制了传统有限差分算子数值模拟中的数值频散现象,提高了有限差分算子精度,为VTI介质频率-空间域qP波正演模拟奠定了基础.     

潮流界以下河段航道整治水位确定方法研究——以长江江阴以下河段为例

在总结分析现有整治水位确定方法的基础上,根据潮流界以下河段的水沙运动特性,本文提出一种基于输沙能力的航道整治水位确定方法。考虑上游来水、下游潮汐为独立事件,统计潮流界以下河段上游来水、下游潮汐不同等级组合出现的频率,采用数学模型计算相应组合下河段沿程的潮位、流速过程,以流速四次方代表水流的输沙能力,统计不同潮位等级对应的综合净输沙能力,确定最大综合净输沙能力对应的水位为(最优)航道整治水位。以长江下游白茆沙水道和福姜沙水道为例,计算了所在河段的航道整治水位,并探讨了起动流速对整治水位计算的影响和最高整治水位概念对工程的意义。     

Reconstruction of eddies by assimilating satellite altimeter data into Princeton Ocean Model

An optimal interpolation assimilation model for satellite altimetry data is developed based on Princeton Ocean Model (POM), which is applied in a quasi-global domain, by the method of isotropic correlation between sea level anomaly (SLA) and sea temperature anomaly. The perfor- mance of this assimilation model is validated by the modeled results of SLA and the current patterns. Comparisons between modeling and satellite data show that both the magnitudes and distribution patterns of the simulated SLA are improved by assimilation. The most signiˉcant improvement is that meso-scale systems, e.g., eddies, are well reconstructed. The evolution of an eddy located in the northwest Paciˉc Ocean is traced by using the assimilation model. Model results show that during three months the eddy migrated southwestward for about 6 degrees before merging into the Kuroshio. The three dimensional structure of this eddy on 12 August 2001 is further analyzed. The strength of this warm, cyclonic eddy decreases with the increase of depth. The eddy shows di?erent horizontal patterns at di?erent layers, and the SLA and temperature ˉelds agree with each other well. This study suggests that this kind of data assimilation is economic and reliable for eddy reconstruction, and can be used as a promising technique in further studies of ocean eddies as well as other ˉne circulation structures.