间接ELISA技术在病原性鳗弧菌SMP1快速检测中的应用

以大菱鲆(Scophthalmus maximus)出血症的病原菌——鳗弧菌(Vibrio anguillarum)SMP1为抗原,制备兔抗血清,测定了抗血清的工作浓度和对抗原的特异性,建立了在大菱鲆肝肾组织中检测SMP1的间接ELISA方法。SMP1人工感染大菱鲆,用建立的间接ELISA对大菱鲆肾脏和肝脏进行检测,结果肾脏的阳性检出率为87.7%,肝脏的阳性检出率为90%。该方法的建立有助于快速准确地鉴定由SMP1引起的大菱鲆疾病。     

新一代天气雷达产品故障识别及监测预警系统设计实现

新一代天气雷达故障直接影响数据质量,异常产品的产生对短时临近预报预警带来了重大的影响,实现雷达异常产品实时监测具有一定现实意义。本系统采用WinForm 3层架构+ORM模型架构,通过统计纹理分析、灰度图像特征值分析等图像处理技术实现自动识别雷达异常产品。新一代天气雷达产品故障监测预警系统可以实时、高效、准确检测出异常雷达产品,满足强对流天气预警监测、天气雷达故障预警服务预判、天气雷达产品可用性服务等功能要求,从单部雷达产品数据中识别出异常回波,避免进一步影响多雷达组网拼图,对天气雷达组网产品的质量控制具有重要的意义。     

Curvature of Flexibility Matrix for Damage Identification in Legs of Jacket Platforms

In this paper a new nondestructive damage identification method is introduced. The method based on flexibility matrix can be used to detect and locate structm＇al damage and evaluate the severity of damage in legs of jacket platforms by modal parameters of a structure. With the modal data for only the few lower modes in both the intact and damaged states, the one-dimensional and two-dimensional distributed curvatures can be used to analyze damage location and the severity. Instead of directly comparing the curvatures before and ＇after damage, the method here uses modal parameters only in the damaged structure to detect the damage and it consists of three parts. First, ilexibility matrix is obtained by use of the absolute maximum in each column. Second, because the legs of jacket platforms are the pipe-like structure, the circumferential flexibility curvature matrix is obtained by use of the circular curvature. At last, equivalent curvature ratio is defined and the curve meaning equivalent curvature ratio and the severity of damage relationship for one element is given through the data of damage severity from ten percent to ninety percent by numerical simulation. Many existing damage detection methods need two steps, locate the damage firstly and evaluate the severity of the damage. However, the method present- ed! in this paper can locate and then evaluate the severity of damage at the same time. The numerical analysis results in- dicate that the present method is effective, useful and only need the first and the second mode data of the structure.     

一种准确而快速的运动目标检测算法

运动目标的检测是计算机视觉和图像编码研究的主要内容,有着广泛的应用领域.减背景法是常用的方法之一,但其受环境光照影响;而帧差法又不能完全提取出目标的所有相关点.针对传统运动目标检测算法的不足,提出基于动态阈值的减背景法与帧差法相结合的运动目标检测算法.实验证明,算法既能完全提取出目标的所有相关点,又适应复杂变化的环境,运动目标检测的精确度和速度都有了明显的提高.     

珠母贝糖胺聚糖胶囊制备与检测

以马氏珠母贝糖胺聚糖粗品为主要功能成分,以β-环糊精为辅料,制成珠母贝糖胺聚糖胶囊,并进行检测。结果表明:该胶囊中水分、总糖、糖胺聚糖(GAG)和粗蛋白质量分数分别为8.85%、85.23%、3.00%和3.60%;胶囊的装量差异与崩解时限均符合质量标准;胶囊制剂中细菌总数小于1000 g-1,大肠杆菌MPN小于30 g-1,霉菌和酵母菌总数小于25 g-1,符合《保健食品通用卫生要求》规定;致病菌均未检出。     

城市活断层控制性浅层地震探测方法分析

在城市活断层探测中,最大的问题是城市干扰和震源问题。为了解决这两个难题,采用了横波、纵波联合探测的方法。对野外数据采集横波激发采用了150 kg冲击锤的机械震源多次叠加,纵波激发采用了Bidirectional,TRacToR9030型3T可控震源多次叠加,取得了良好的地质效果。     

Application of clan member signal method in structural damage detection

It is well known that in most cases, a reference is necessary for structural health diagnosis, and it is very difficult to obtain such a reference for a given structure. In this paper, a clan member signal method (CMSM) is proposed for use in structures consisting of groups (or clans) that have the same geometry, i.e., the same cross section and length, and identical boundary conditions. It is expected that signals measured on any undamaged member in a clan after an event could be used as a reference for any other members in the clan. To verify the applicability of the proposed method, a steel truss model is tested and the results show that the CMSM is very effective in detecting local damage in structures composed of identical slender members.     

广东省粤西地区凡纳滨对虾3种主要病毒的PCR与LAMP检测及流行病学特点分析

应用聚合酶链式反应(Polymerase Chain Reaction,PCR)和环介导等温扩增(Loop-Mediated Isothermal Ampli-cation,LAMP)两种检测技术分别对2009年广东省粤西地区养殖体系中凡纳滨对虾Litopenaeus vannamei白斑综合症病毒(White Spot Syndrome,WSSV)、桃拉病毒(Taura Syndrome Virus,TSV)和传染性皮下及造血组织坏死病毒(Infectious Hypodermal and Hematopoietic Necrosis Virus,IHHNV)的携带情况进行了调查。结果显示,WSSV和TSV的携带率较高,是该地区凡纳滨对虾的主要流行病毒种类;LAMP检测方法与PCR方法具有相当的灵敏度和特异性,但LAMP检测方法更加简单方便、快速且成本低。     

利用流式细胞计进行虾类倍性检测的研究

在生物有机体中,一个细胞核中含的ＤＮＡ总量对于某种类有机体是一定的。利用流式细胞计测量细胞中的ＤＮＡ含量,可以和来鉴别有机体的倍性,本文处次报道了用流式细胞计测定中国对虾（Ｐｅｎａｅｕｓｃｈｉｎｅｎｓｉｓ）,日本对虾（Ｐ．ｊａｐｏｎｉｃｕｓ）和刀额新对虾（Ｍｅｔａｐｅｎａｅｕｓｅｎｓｉｓ）倍性的结果。     

国外港口物流发展与闽台港口物流对接

分析福建与台湾港口物流发展现状和存在问题，以及高雄-厦门、高雄-福州海上试航和“两门两马”小三通直航发展态势，指出两岸试航的局限性和港口物流直航的必然性。综合分析鹿特丹、新加坡、安特卫普、釜山、汉堡等世界著名港口物流发展经验，提出对闽台两岸港口物流对接具有可供借鉴经验。针对闽台港口物流发展中存在问题提出了两岸港口资源整合，建立台海港口群；完善集疏运体系，促进港口物流对接；大力发展临港工业，构建环台湾海峡临港工业走廊等措施。