水中荧光计及其在海洋现场探测中的应用

本文描述了近几年来我们首次研制成功的３种类型的水中荧光计系统及其应用测量结果：１．用于水体混合扩散实验研究的水中荧光计，对罗丹明Ｂ浓度测量范围为１×１０－６～１×１０－１０ｇ／ｃｍ３；２．拖曳荧光计系统，能在２～８０ｍ水深内进行水平拖曳平稳地工作，拖曳速度为０～８ｋｎ，除检测罗丹明Ｂ外，还可检测无机悬浮物质的含量，检测范围为２～２０００ｍｇ／ｄｍ３；３．用于测量海中浮游植物体内叶绿素ａ浓度的水中荧光计，测量范围为１×１０－６～１×１０－９ｇ／ｃｍ３。用荧光法测量海中物质是近代新发展的一种简便、快速、灵敏度高、用途极其广泛的测量技术，有着重要的应用价值。     

Estuary/ocean exchange and tidal mixing in a Gulf of Maine Estuary: A Lagrangian modeling study

A Lagrangian particle method embedded within a 2-D finite element code, is used to study the transport and ocean–estuary exchange processes in the well-mixed Great Bay Estuarine System in New Hampshire, USA. The 2-D finite element model, driven by residual, semi-diurnal and diurnal tidal constituents, includes the effects of wetting and drying of estuarine mud flats through the use of a porous medium transport module. The particle method includes tidal advection, plus a random walk model in the horizontal that simulates sub-grid scale turbulent transport processes. Our approach involves instantaneous, massive [O(500,000)] particle releases that enable the quantification of ocean–estuary and inter-bay exchanges in a Markovian framework. The effects of the release time, spring–neap cycle, riverine discharge and diffusion strength on the intra-estuary and estuary–ocean exchange are also investigated.The results show a rather dynamic interaction between the ocean and the estuary with a fraction of the exiting particles being caught up in the Gulf of Maine Coastal Current and swept away. Three somewhat different estimates of estuarine residence time are calculated to provide complementary views of estuary flushing. Maps of residence time versus release location uncover a strong spatial dependency of residence time within the estuary that has very important ramifications for local water quality. Simulations with and without the turbulent random walk show that the combined effect of advective shear and turbulent diffusion is very effective at spreading particles throughout the estuary relatively quickly, even at low (1 m²/s) diffusivity. The results presented here show that a first-order Markov Chain approach has applicability and a high potential for improving our understanding of the mixing processes in estuaries.     

Robust adaptive control of underactuated ships on a linear course with comfort

This paper addresses an important problem in ship control application—the robust stabilization of underactuated ships on a linear course with comfort. Specifically, we develop a multivariable controller to stabilize ocean surface ships without a sway actuator on a linear course and to reduce roll and pitch simultaneously. The controller adapts to unknown parameters of the ship and constant environmental disturbances induced by wave, ocean current and wind. It is also robust to time-varying environmental disturbances, time-varying change in ship parameters and other motions of the ship such as surge and heave. The roll and pitch can be made arbitrarily small while the heading angle and sway are kept to be in reasonably small bounds. The controller development is based on Lyapunov’s direct method and backstepping technique. A Lipschitz continuous projection algorithm is used to update the estimate of the unknown parameters to avoid the parameters’ drift due to time-varying environmental disturbances. Simulations on a full-scale catamaran illustrate the effectiveness of our proposed controller.     

东亚和东南亚第四系时间界面的建议

在东亚和东南亚第四系对比中,有关的几条界限,本文建议如后。采用Globorotalia truncatulinoides的初现位作为本区海相第四系的下界,其位置大致在古地磁Olduvai亚时的下界,时间约为距今1.9Ma。在南中国海、东海及沿岸地区,发现了以G.truncatulinoides的初现位为代表的一系列有意义的生物事件,如Pulleniatina finalis的初现位,Globigerinoides obliquus和Globoquadrina altispira的灭绝位,以及Globorotalia menardii和Pulleniatina obliquloculata壳体旋向的变化。此界限在东亚和东南亚也是相当稳定的。在陆相地层中,以泥河湾组为代表,狭义的泥河湾动物群(相当于晚维拉方动物群)所在地层的底界,其古地磁位置为Olduvai亚时的底界。以此作为陆相第四系的下界,与上述海相第四系底界一致。 以古地磁布容/松山界线作为中更新世和早更新世的界限,其年代为0.73MaB.P.。中国中更新世的标准地层周口店组,其底界恰为布容/松山的转换面,北京猿人的年代为0.60—0.23MaB.P.。在洛川黄土剖面上,布容/松山界线为古土壤S_7的底界。雷琼地区中更新统下部的北海组的底界也是布容/松山界线,所产的玻璃陨石的裂变径迹年龄为0.687—0.733MaB.P.。在黄海,布容/松山界线是第Ⅶ海侵层的顶界。通过岩石地层标志,以及动物群、古人     

围垦区地下水位的控制及计算方法

利用抽水井群和注水井群可以控制生态围垦区的地下水位.研究了潜水抽-注水井(群)耦合作用下浸润线的求解方法,利用势流理论和镜像原理,在假定含水层水平及土层渗透系数各向同性的条件下,推导出完整抽-注水井(群)耦合作用时浸润线的计算公式及非完整抽-注水井(群)耦合作用时浸润线的计算公式,还对潜水单个非完整抽水井及非完整抽-注水井群耦合作用时的解析解和数值解作了比较,结果表明,两者吻合得较好.同时还给出该理论用于地下水位控制中求解抽水量和注水量的方法等,对工程设计有实用价值.     

Ekman螺线的动力学结构

介绍了Ekman螺线的动力学结构 ,分析研究了风应力及海水内部的动量传递形式 .结果表明 :动量传递方向与海流方向成 45°左偏 .这对于研究海洋内部的动力结构是有意义的 .     

珠江口伶仃洋锋的类别及其对沉积的影响

根据近年在珠江口伶仃洋的观测资料，对比国外有关河口锋的定义，归纳了伶仃洋内几种锋的现象，计有潮侵锋、岬角锋及湖水退急时的浅滩锋等。解释了锋生的原因与潮汐进退，特别与随潮入侵的咸水关系。文中也探讨了这些锋对沉积环境的影响，认为它是陆架水入侵河口地区的一种不容忽视的沉积动力过程。     

利用牙鲆鳃细胞系分离和培养淋巴囊肿病毒

本文利用牙鲆鳃细胞系进行了养殖牙鲆淋巴囊肿病毒的分离及培养 ,并通过电镜对培养细胞中淋巴囊肿病毒的形态及感染循环进行了初步研究。将病鱼的淋巴囊肿组织无菌滤液接种牙鲆细胞系 ,细胞出现了明显的细胞病变 ( Cytopathic effect,CPE)。电镜观察在培养细胞的胞质中有病毒的包涵体 ,胞质中散在 6角形、5角形或圆形的病毒粒子 ,大小为 10 0～ 140 nm之间。在感染细胞的线粒体中也存在大量的病毒颗粒。     

Effects of organochlorine compounds on cytochrome P450 aromatase activity in an immortal sea turtle cell line

Many classes of environmental contaminants affect the reproductive function of animals through interactions with the endocrine system. The primary components affected by endocrine active compounds (EACs) are the steroid receptors and the enzymes responsible for steroidogenesis. This study sought to develop an in vitro model for assessing EAC effects in sea turtles by examining their ability to alter cytochrome P450 aromatase (CYP19) activity. Aromatase is the enzyme responsible for the conversion of testosterone to estradiol. This enzyme is critical in the sexual differentiation of reptiles which demonstrate temperature-dependent sex determination. An immortal testis cell line GST-TS from a green sea turtle was grown in culture at 30 degrees C in RPMI 1640 media. The cells were exposed to three known aromatase inducers; dexamethasone (Dex), 8Br-cyclic AMP, or human chronic gonadotropin (HCG) and one aromatase inhibitor 4-androstenol-dione (4-OHA). In addition, the GST-TS cells were exposed to 0.1-30 microM atrazine and 3-100 microM 4,4'-DDE. The inducing compounds that have been shown to increase aromatase activity in other systems failed to induce aromatase activity in the GST-TS cells, yet exposure to the inhibiting compound, 4-OHA, did result in a significant reduction. Atrazine (0.1, 1.0 and 10 microM) significantly induced aromatase activity following a 24 h exposure, and 4,4'-DDE inhibited the activity but only at cytotoxic concentrations (100 microM). Based on these results, this in vitro model can be useful in examining the endocrine effects of EACs in sea turtles.