光照强度对海洋微藻脂肪含量及脂肪酸组成影响的研究

近年来，海洋微藻脂肪酸组成的研究及其应用越来越受到国内外科学家的重视。高度不饱和脂肪酸(PUFA)，特别是长链的n-3 PUFA，如二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)对海洋动物和人类都具有营养学和医学上的价值。在海洋微藻的培养过程中，光照强度是海洋微藻生长的条件之一，光线的明与暗，光度的强与弱，不仅对微藻的生长速率、产量有影响，而且对其脂肪含量和脂肪酸的组成也有影响(Teshima et al.，1983；Thompson et al.，1990； Renaud et al., 1991)。 作者在以往研究的基础上，选择了3种有代表性的海洋微藻：(1)小球藻Chlorella sp-2(李荷芳等，1999)，此藻脂肪酸中EPA含量高、且不含DHA；(2)球等鞭金藻(Isochrysis galbana)，该藻DHA含量较高，但几乎不含EPA；(3)前沟藻(Amphidinium sp.)的EPA、DHA含量均高。将以上3种微藻作为原料，在不同的光照强度下进行培养，测定并分析藻体中的脂肪含量和脂肪酸组成的变化，从而了解光强对海洋微藻脂肪含量及其脂肪酸组成的影响。     

温度、光强和盐度对鹧鸽菜幼体生长发育的影响

从野外采集鹤鸪菜Ｃａｌｏｇｌｏｓｓａｌｅｐｒｉｅｕｒｉｉ（Ｍｏｎｔ．）Ｊ．Ａｇ．幼体，在室内控制条件下，以温、盐、光等不同条件进行模拟培养试验。所获得的数据经生物统计检验表明：鹧鸪菜幼体生长最适水温为２３℃；光照强度为４０００ｌｘ；海水盐度为１８。此结果与我们多年来在汕头港内观察鹧鸪菜的生长及其季节变化相似。     

A new approach for predicting the extreme response of structures

In this article a method to estimate the mean upcrossing intensity, μ(u), of a stochastic process is proposed. It is assumed that the stochastic process is a sum of a Gaussian process and a second-order correction term. The method is based on the two-dimensional Saddlepoint approximation. The accuracy of the method is tested on processes having analytical solutions for μ(u). Numerical examples are given where the stochastic process represents (i) the horizontal response of a floating offshore structure in a Gaussian sea, and (ii) the response of a structure subjected to a Gaussian wind velocity process. In addition, the estimates are compared to empirical upcrossing intensities of simulated responses. For case (ii), the obtained μ(u) estimates are compared to estimates obtained by numerical integration.     

Phytoplankton production in two east coast estuaries: Photosynthesis-light functions and patterns of carbon assimilation in Chesapeake and Delaware Bays

Chesapeake Bay is a large and productive estuary that has received close scrutiny in recent years because of indications that its water quality and biota have been damaged by man's activities. Data on primary production for the estuary as a whole, however, are surprisingly sparse. We describe here the distribution of photosynthetic carbon assimilation by phytoplankton in Chesapeake Bay, and relate productivity patterns to hydrographic characteristics of the estuary. Between March 1982 and April 1983, a series of four cruises was conducted on Chesapeake Bay, and two cruises on the urbanized Delaware Bay for comparison. The upper Chesapeake and Delaware were highly turbid with high concentrations of suspended particulate matter and dissolved inorganic nutrients. Low chlorophyll concentrations were usually found in these areas of high turbidity, despite the abundance of nutrients, suggesting light limitation. Application of Wofsy's (1983) model of phytoplanton growth confirmed this suggestion. Chlorophyll and productivity maxima usually occurred seaward of the turbidity maxima where light penetration increased and suffient nutrients were present to support active phytoplankton growth. Further seaward of the chlorophyll maxima in the Chesapeake, the photic zone depth increased, concentrations of nutrients decreased, and phytoplankton biomass decreased, suggesting that nutrient availability, rather than light, controlled phytoplankton growth in the lower portion of the estuary. In contrast to the Chesapeake, Delaware Bay was more turbid, had generally higher nutrient concentrations, and was lower in phytoplankton productivity. The chlorophyll maxima and region of rapid phytoplankton growth occurred further toward the lower estuary and shelf regions in Delaware Bay because the high turbidity extended further seaward. Nutrients were never depleted at the shelf end of the estuary sufficiently to retard phytoplankton growth. Photosynthesis-irradiance (P-I) curves from simulated in situ and constant intensity incubations showed a strong correlation of the light-limited slope (a^B) with the light-saturated rate ( ) on each cruise. Spatial variations in corresponded to patterns of phytoplankton abundance, as did integral production (PP) and carbon-based growth rates (μ_C, μ_m), and photosynthetic parameters varied significantly with temperature.     

激光扫描共聚焦显微镜在海洋动物发育生物学上的应用

激光扫描共聚焦显微镜具有成像清晰、获得三维图像、进行多标记观察等优势。本文扼要介绍了激光扫描共聚焦显微镜的原理和特点,着重阐述了激光扫描共聚焦显微镜在海洋动物配子发生;受精过程中细胞内Ca~(2 )变化、肌动蛋白转移和蛋白激酶变化;胚胎和幼体发育的三维成像等研究领域中的应用。并对LSCM在DNA含量、RNA含量等领域的定量、定性前景进行展望。     

礁膜配子放散条件的研究

本文报道了礁膜配子的放散与温度、光照、比重的关系。结果表明，自然海区的礁膜在白天大量放散配子。配子放散的适宜温度为１９－２１℃；光照为２０００－５０００ｌｘ时能促进配子大量放散出来；配子放散的适宜比重为１．０５－１．０２５；这些研究结果将为礁膜人工育苗提供一些科学依据。     

哀牢山-红河断裂带中段应变分析

对哀牢山—红河断裂带中段和平—水塘剖面、墨江—元江剖面和其它地段的岩石应变及磁组构进行了分析，表明应变强度的校正磁各向异性度PJ从断裂带向西至三叠系明显降低，变形强度向西迅速减弱。磁化率椭球体主轴展布反映出剪切带内、外变形方式的改变。剪切带内，以水平走滑运动为主；向西则以水平缩短为主。在应变分析中，对断裂带内的S—C组构两组面理夹角、杏仁体和石榴石应变标志体进行了测量，结果显示，剪切带内的磁组构和岩石组构间关系较差。此外，还对这两条剖面中的三叠系进行了应变和磁组构分析，找出了它们与磁化率椭球体对应轴率间的相关性。     

不同激光扫描数据组拼接的一种新方法

激光扫描测量技术在三维城市建模领域的应用具有良好的发展前景。但是,不同精度的扫描数据组的拼接是目前需要解决的主要问题之一,本文对不同精度的数据组的拼接进行了实验性研究,并提出了一种新方法。     

激光扫描点云图像背景交互式滤除

激光扫描系统获取的数据中包含了大量背景信息,给信息提取和表面重建工作带来严重影响,需要有效滤除。依据空间点与平面的位置关系,提出了针对车载激光扫描点云图像背景信息滤除的有效方法,并以OpenGL为工具,开发了一系列点云数据处理工具,能快速、直观、准确地滤除无用背景信息,完整地保留有用的前景信息,从而为大规模三维场景快速重建提供保障。