Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis)

A new cell line,CSEC,has been successfully established from embryos at gastrula stage of a cultured marine fish,half smooth tongue sole(Cynoglossus semilaevis).CSEC cells grow actively and stably more than 50 passages for over 200 d in DMEM medium supplemented with 15% FBS(fetal bovine serum),2.5 ng/cm 3 bFGF(basic fibroblast growth factor),1 ng/cm 3 LIF(leukemia inhibitory factor) and 50 mmol/dm 3 2-ME(2-mecaptoethanol).The cells grew well in the temperature range of 24-30 ℃ and the optimal growth temperature was 24℃.FBS and bFGF concentrations are the two key components for CSEC cells proliferation.Chromosome analysis reveals that CSEC cells have a normal diploid karyotype with 2n=42t.The significant fluorescent signals were observed in CSEC after transfection with the GFP reporter gene,suggesting that the CSEC cell line can be used as a useful tool for transgenic and genetic manipulation studies.CSEC cells showed the cytopathic effect(CPE) after infection with lymphosystis disease virus(LCDV) in 2 d.Moreover,the LCDV particles can be observed in the cytoplasm of virus-infected cells by electron microscopy.It suggests that CSEC could be potentially used for the study of aquatic virus.     

An interconnect width and spacing optimization model considering scattering effect

As the feature size of the CMOS integrated circuit continues to shrink, the more and more serious scattering effect has a serious impact on interconnection performance, such as delay and bandwidth. Based on the impact of the scattering effect on latency and bandwidth, this paper first presents the quality-factor model which optimises latency and bandwidth effectively with the consideration of the scattering effect. Then we obtain the analytical model of line width and spacing with application of curve-fitting method. The proposed model has been verified and compared based on the nano-scale CMOS technology. This optimisation model algorithm is simple and can be applied to the interconnection system optimal design of nano-scale integrated circuits.     

印度河上游Bagrot山谷降水稳定同位素变化及与水汽来源的关系

利用2015年8月至2016年7月在印度河上游流域Bagrot山谷降水稳定同位素（δ18O和δD）观测结果以及当地气象资料,利用同位素示踪及统计分析方法,并结合HYSPLIT模型,对研究区降水稳定同位素变化特征、大气水线以及水汽来源进行了分析。结果表明,观测期间Bagrot山谷降水稳定同位素的季节变化明显,δ18O与δD秋冬季偏低,春夏季偏高,且与气温变化一致,存在显著的温度效应,而降水量效应不明显。而且发现,研究区局地大气水线截距和斜率均低于全球的,反映了降水过程中云下二次蒸发作用较为强烈,特别是,不同的降水形态导致该研究区局地大气水线的斜率和截距不同。当液态降水（降雨）发生时,由于在较为干旱的气候环境下,雨滴在降落的过程中受到二次蒸发相对较强,使得局地大气水线的斜率和截距偏低;而当固态降水（降雪）发生时,由于温度较低,受再循环水汽和二次蒸发的影响较小,导致局地大气水线的斜率和截距均偏高。Bagrot山谷及其周边地区,从南到北局地大气水线的斜率相差不大,而其截距总体上随着纬度升高而降低,可能与云下二次蒸发导致稳定同位素发生的不平衡分馏逐渐强烈有关。通过Bagrot山谷站点降水稳定同位素观测结果并结合HYSPLIT模型的后向追踪,研究还发现,研究区全年主要受西风环流以及局地环流的影响。但与研究区以北的临近站点（慕士塔格、和田等）相比有所不同,由于Bagrot山谷位置更靠南,其仍然偶尔受到来自南方的海洋性水汽影响。这一研究结果可能对该地区树轮稳定同位素记录的解译具有一定的指示意义。     

关于确保甘肃省祁连山生态保护红线落地并严守的科技支撑建议

祁连山是中国西部重要生态安全屏障,是国家重点生态功能区,其生态保护与治理一直受到国家、地方政府和当地居民高度关注。目前,在祁连山的生态保护工作中,缺乏对现状基础数据的全面掌握,区域经济发展与生态环境保护矛盾突出,生态环境综合监测系统不完备,生态补偿机制推进实施缺乏创新。在全面梳理祁连山目前存在的重大生态问题的基础上,提出了祁连山生态保护红线落地并严守的建议,包括加快开展祁连山综合科学考察、完善并落实开展生态补偿机制、探索以正面清单为导向的协调发展模式、促进生态安全与地方经济社会可持续发展相协调、进一步实施重大配套政策、完善保障支撑体系等。     

基于长时间序列遥感数据的阿伯德尔国家公园 生态环境质量评价

阿伯德尔国家公园位于号称肯尼亚“水塔”的阿伯德尔山脉上,是肯尼亚重要的旅游景区和珍稀动植物保护区,开展该地生态环境质量评价对肯尼亚水资源保护与可持续发展具有重要的意义。本研究基于1987-2018年的Landsat系列遥感数据,通过计算综合遥感生态指数RSEI,结合趋势线分析法对阿伯德尔国家公园近32年间生态环境质量变化进行客观定量分析。研究结果显示：① 1987-2018年研究区RSEI均值从0.62下降到0.51,生态环境质量退化的区域面积为551.52 km ²,占总面积的71.85%,表明在气候变化和人类活动影响下,1987-2018年研究区的环境状况呈恶化趋势;②环境质量显著恶化的区域主要分布于亚高山植被区和东部低海拔森林覆盖区,亚高山地区植被生态系统结构单一,稳定性差,对气候变化和人类活动更为敏感;而东部区域存在过度开发,非法砍伐森林等现象,且旅游开发强度大,环境恶化与人类活动密切相关。     

GNSS多天线基线网单历元模糊度同步解算法

提出多天线基线网单历元模糊度同步解算法,其具体实现步骤为:1)根据宽巷组合模糊度易于固定的优点,采用附加已知基线长度约束法同步解算各基线的宽巷模糊度,得到dm～cm级精度的近似基线分量;2)将解算得到的各近似基线分量作为约束,同步解算各基线的基频模糊度,以获取mm级精度的基线矢量。该方法的关键在于检验各历元宽巷模糊度解算的正确性,以获取可靠的近似基线分量,为解算各基线的基频模糊度提供准确的基线先验信息。由于动态情况下各历元观测信息比较少,单纯依赖ratio检验不可靠,提出结合基线误差、单位权中误差、基线网模糊度闭合环及ratio值等对多组宽巷模糊度进行检验,避免ratio值设置不当导致模糊度检验中发生纳伪和弃真问题。实测数据结果证明,该处理方法使得模糊度解算的成功率提高1%～2%,可以获取移动平台更丰富的导航信息,提高其服务能力。     

加权EIV模型的经典最小二乘算法

采用GHM方法将EIV模型在最优解处线性化,得到解的近似方差。然后,将EIV模型表达成与Gauss-Markov模型相似的形式,利用标准最小二乘理论推导EIV模型的解及近似方差矩阵,得到与已有算法等价的结论。最后,推导观测值估值和残差的统计性质,建立起一整套EIV模型参数估计和精度评定的体系。     

Distinct activity phases during the recent geologic history of a Gulf of Mexico mud volcano

Laser line scan imaging and chirp sub-bottom profiling were used to detail the morphology of a submarine mud volcano and brine-filled crater at 652 m water depth in the northern Gulf of Mexico. The mud volcano has a relief of 6 m and a basal diameter of about 80 m. The feature comprises a central, brine-filled crater (253 m²) surrounded by a continuous bed of methanotrophic mussels (Bathymodiolus childressi) covering 434 m² and a patchy bed covering an additional 214 m² of the periphery. The brine pool was mostly <2 m deep, but there were two holes of >28 m and 12 m deep, respectively at the northern end of the pool which emitted continual streams of small clear bubbles. Sub-bottom profiles indicated three distinct strata beneath the present surface of the mud volcano. Integration of 17 profiles shows that the mud volcano has been built in at least three successive stages: the lowest stage deposited 35,400 m³, while the middle and upper stages deposited 7700 and 20,400 m³, respectively. Piston cores were taken at the northern edge of the mussel bed and a site ∼100 m southwest of the pool. Mussel and lucinid shells were recovered from the closer core, lucinid shells from the distant core. A mussel shell from 3.4 m sub-bottom had a Δ¹⁴C age of 16.2 ka. Mixture of modern carbon with “carbon dead” reservoir material would produce actual ages ∼2 ka less than the radiocarbon ages.     

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康普顿化的计算-推广的Kompaneets方程与Monte Carlo方法比较

在X射线天文学以及辐射物理学中,当硬X射线穿过" 冷"的等离子体时所发生的Compton软化是一种重要的辐射转移过程.简要介绍推广的Kompaneets方程,该方程在hv〈mec2及kTe〈mec2较宽松的条件下广泛成立,因此不仅能处理Compton硬化过程,而且也适用于Compton软化过程,后者在目前快速发展的X射线和γ射线天文学中十分重要.基于此方程,我们对天体物理中4种常见辐射谱(Gauss型发射谱线、黑体辐射谱、幂律辐射谱和热轫致辐射谱)在Compton软化情况下的谱演化进行了数值求解,并同Monte Carlo模拟结果做比较,证实了推广的Kompaneets方程的正确性和有效性.最后指出此方程在X射线天文学和γ射线天文学中的重要意义和潜在应用.