对虾白斑综合征病毒VP28基因在大肠杆菌中的表达

在已构建重组质粒pGEM—vp28的基础上,通过NcoⅠ,HindⅢ双酶切质粒pGEM—vp28和表达载体质粒pET-30a,分别获得携带限制性内切酶粘性末端的vp28基因片段和表达载体质粒片段,两片段经T4DNA连接酶连接获得了重组表达质粒pET30a—vp28,将此重组质粒导入表达宿主菌Escherichia coli BL21中,用IFIG诱导表达4h后,其表达产物在SDS—PAGE和Western blot中的显示的特异性条带大小约27ku,与预期的大小相近。     

武陵源风景名胜区旅游发展的动力学分析

根据Butler旅游地生命周期理论,利用历史数据统计,分析了武陵源风景名胜区主要景区生命周期的动态演化趋势。实证研究表明,卫星景区宝峰湖和黄龙洞景区正处于发展阶段,成熟阶段是今后理想演进阶段。武陵源核心景区已处于成熟稳定阶段,要严格控制游客数量,调整旅游产品结构,避免滑向衰落阶段。最后,对武陵源风景名胜区旅游环境容量控制问题进行了探讨。     

太阳黑子穿过同磁极性大黑子的观测

本文利用紫金山天文台精细结构望远镜拍摄的优质Ｈａ线心和离带照片，分析了新浮黑子活动区１９９０年１２月５日至９日磁结构演化及黑子的自行运动。我们观测到了一黑子穿过另一同极性大黑子这一非常少见的现象。     

鄂西盐池河温泉成因及其对水布垭水利工程地壳稳定性的影响

盐池河温泉是清江流域内唯一的温泉，出露于奥陶系红花园组灰岩地层中，受控于龙王冲断裂派生出的ＮＮＥ、ＮＷ向次级小断层。地质分析及有关测试成果表明，该温泉水源主要来自大气降水和清江河水沿断裂的下渗，经地热增温并吸收龙王冲断裂稳滑运动所产生的摩擦热量后，再沿ＮＮＥ、ＮＷ向断裂交汇部位溢出地表，是一个地层一断层复合控制型温泉。该温泉的出露，有利于水布垭水利工程区域地壳稳定性，可以减少水库诱发地震的机会。     

α2 CVn: A comparison between spot and oblate spheroid models

A comparison is made between a hot-spot model and a recently proposed oblate spheroid model (Böhm-Vitense & Van Dyk 1987) to explain the spectroscopic and photometric variations of α² CVn. It is found that the spot model gives a better fit to the spectroscopic and photometric variations. The spot model requires five high temperature circular patches over the surface of the star. The positions of these patches agree well with those derived spectroscopically by Pyper (1969).     

丹阳嘉山风景区废弃宕口地质环境治理可行性研究

分析及阐述了丹阳市嘉山风景区废弃宕口地质环境治理工作的目的、意义,明确了工程治理原则和总体目标,从治理方法、规划方案、项目保障及效益分析等方面进一步论证了项目实施的可行性和重要性。     

Geochemical and Microbiological Analysis of Sambe Hot Springs,Shimane Prefecture,Japan

Microbiological contribution to the formation of the manganese deposits in Sambe hot springs, Shimane, was investigated in combination with water chemistry, characterization of sediments and microbial community structure. Analysis of bacterial and fungal community structure based on DNA extracted from a Mn‐oxidizing enrichment culture indicated close matches with Pseudomonas putida, Phoma sp. and Plectosphaerella cucumerina, all Mn‐oxidizing microorganisms. These sediments were poorly crystalline and formed at neutral pH values, which is characteristic of biogenic precipitates. The EPMA results demonstrated a positive correlation between Mn and Ba contents in well‐crystalline Mn oxide grains. Substantial Ba contents were observed inside Mn oxide grains. These findings indicated that Ba contents in sediments are influenced by not only aqueous Ba²⁺ concentrations but also crystallinity of biogenic birnessite. Barium would be incorporated in birnessite during biomineralization.     

Causes of the Extreme Hot Midsummer in Central and South China during 2017:Role of the Western Tropical Pacific Warming

This study investigates why an extreme hot midsummer occurred in Central and South China(CSC) during 2017. It is shown that the western North Pacific subtropical high(WNPSH) was abnormally intensified and westward-extending,resulting in anomalous high pressure and consequent extreme heat over CSC. The abnormal WNPSH was favored by the warming of the western tropical Pacific(WTP), which was unrelated to ENSO and manifested its own individual effect.The WTP warming enhanced the convection in-situ and led to anomalous high pressure over CSC via a local meridional circulation. The influence of the WTP was confirmed by CAM4 model experiments. A comparison between the 2017 midsummer and 2010 midsummer(with a stronger WNPSH but weaker extreme heat) indicated that the influence of the WNPSH on extreme heat can be modulated by the associated precipitation in the northwestern flank.The role of the WTP was verified by regression analyses on the interannual variation of the WTP sea surface temperature anomaly(SSTA). On the other hand, the WTP has undergone prominent warming during the past few decades, resulting from decadal to long-term changes and favoring extreme warm conditions. Through a mechanism similar to the interannual variation, the decadal to long-term changes have reinforced the influence of WTP warming on the temperature over CSC,contributing to the more frequent hot midsummers recently. It is estimated that more than 50% of the temperature anomaly over CSC in the 2017 midsummer was due to the WTP warming, and 40% was related to the decadal to long-term changes of the WTP SSTA.     

大同地区干热岩勘查高温高压自喷井综合治理工艺

在大同天镇干热岩勘查项目中,当钻进至1622 m时,井内突然涌出大量高温高压气水混合物,这在干热岩钻井工作中极其罕见。针对这一中东部地区深部地热资源的重大发现,山西地勘局组织专家分析研究井口涌出情况,通过反复实践,利用方箱开合闸板实现引流,并进一步运用“下钻—注浆—降温—减压—压井—安装采油树”综合治理工艺,成功地完成了这眼地热井的保井发电试验任务。本文分析了自喷井形成原因,系统地总结了整个治理工艺过程,为今后同类型地热资源勘查钻探工作提供宝贵的经验和技术支撑。     

Hydraulic Conductivity Characteristics of Hot Dry Rocks under Different Fracture Modes

Enhanced geothermal system (EGS) is an effective method for developing and utilizing hot dry rock (HDR). The key to the effectiveness of EGS is the construction of an artificial fracture network. The permeability of fractures has severe effects on the heat transfer efficiency and sustainability of geothermal energy. However, the evolution characteristics of hydraulic conductivity under different failure modes have not been adequately studied for HDR. To clarify this, rocks with different failure modes were investigated by conducting thermal triaxial compression experiments, and the fluid seepage related to different rock failure modes was comprehensively investigated. The results showed that the characteristic stresses and crack surface roughness of the rock increased as the confining pressure increased. The permeability in the composited failure mode was the largest (11.4 μm2), followed by that in the Y-shaped shear failure mode (9.7 μm2), and that in the single-shear failure mode was the smallest (7.2 μm2). The confining pressures had an inhibitory effect on permeability. As the confining pressure increased from 5 to 30 MPa, the permeability decreased by 88.8%, 88.4%, and 89.9%, respectively. In contrast, the permeability was significantly enhanced by 128.3%, 94.6%, and 131% as the flow rate increased from 3 to 7 mL/min.