Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of ≈49 kDa size, and weak signals for proteins of ≈41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of ≈56 and 59 kDa size, and also a weak immunoreaction with a protein of ≈43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14–40 ng/g DW in controls to 373–638 ng/g DW in exposed mussels.     

A note on estimation of the Jacobian elliptic parameter in cnoidal wave theory

A new and simple calculating technique for the Jacobian elliptic parameter is presented in this study. The technique is very useful in generating a train of cnoidal waves in both laboratory and numerical wave tanks. Upon specification of water depth, the wave height and either the wave period or the wavelength, the proposed technique uses the Newton–Raphson method to estimate the Jacobian elliptic parameter directly, without trial and error procedures or look-up in tables. It is shown that the technique provides equally accurate results as the ad hoc methods previously used.     

基于EOF分析对南海西北海域水体光谱特性的研究

This study presents an analysis of the spectral characteristics of remote sensing reflectance(R_(rs)) in northwestern South China Sea based on the in situ optical and water quality data for August 2018.R_(rs)was initially divided into four classes,classes A to D,using the max-classification algorithm,and the spectral properties of whole R_(rs) were characterized using the empirical orthogonal function(EOF) analysis.Subsequently,the dominant factors in each EOF mode were determined.The results indicated that more than 95% of the variances of R_(rs) are partly driven by the back-scattering characteristics of the suspended matter.The initial two EOF modes were well correlated with the total suspended matter and back-scattering coefficient.Furthermore,the first EOF modes of the four classes of R_(rs)(A-D R_(rs)-EOF_1) significantly contributed to the total variances of each R_(rs) class.In addition,the correlation coefficients between the amplitude factors of class A-D R_(rs)-EOF_1 and the variances of the relevant water quality and optical parameters were better than those of the unclassified ones.The spectral shape of class AR_(rs)-EOF_1 was governed by the absorption characteristic of chlorophyll a and colored dissolved organic matter(CDOM).The spectral shape of class B R_(rs)-EOF_1 was governed by the absorption characteristic of CDOM since it exhibited a high correlation with the absorption coefficient of CDOM(a_g(λ)),whereas the spectral shape of class C R_(rs)-EOF_1 was governed by the back-scattering characteristics but not affected by the suspended matter.The spectral shape of class D R_(rs)-EOF_1 exhibited a relatively good correlation with all the water quality parameters,which played a significant role in deciding its spectral shape.     

The Bering Sea—A dynamic food web perspective

The Bering Sea is a high-latitude, semi-enclosed sea that supports extensive fish, seabird, marine mammal, and invertebrate populations and some of the world's most productive fisheries. The region consists of several distinct biomes that have undergone wide-scale population variation, in part due to fisheries, but also in part due to the effects of interannual and decadal-scale climatic variation. While recent decades of ocean observation have highlighted possible links between climate and species fluctuations, mechanisms linking climate and population fluctuations are only beginning to be understood. Here, we examine the food webs of Bering Sea ecosystems with particular reference to some key shifts in widely distributed, abundant fish populations and their links with climate variation. Both climate variability and fisheries have substantially altered the Bering Sea ecosystem in the past, but their relative importance in shaping the current ecosystem state remains uncertain.     

海底管道开沟机械的技术经济性能评定与选型

本文在对水下管道开沟机械的需求市场、实际应用和研制开发动向作深入调研的基础上，选择水下管道开沟机械特有的技术先进性、适用性、安全可靠性以及经济性评价指标，构造评价模型，对水下管道施工作业中广为应用的水力冲射开沟机械、海底管道犁、机械开沟机和土壤液化法埋管设备及其机型的技术经济性能作了较为详尽地分析对比及综合评价。在此基础上，笔者提出了水下管道开沟机械的一般选型原则和对我国海底管道开沟机械研究开发工     

白沙河地下水资源管理模型

针对白沙河地下水含水层的内部结构和本区开采特点以及已出现的环境问题，建立了地下水资源管理模型，确定了优化开采方案。该管理模型针对本区每年２个管理时段的长度及其开采量均不同的特点，导出了新的响应矩阵的公式。管理模型的建立有助于对该区地下水的合理开采。     

吉尼系数的计算及正态分布的吉尼系数

本文给出了吉尼系数的一般计算公式 ,推导出正态分布的吉尼系数渐进无偏估计 ,并以实例对比了传统的吉尼系数的算法——万分法与本文中提出的估计方法。     

Measurements of higher harmonic wave forces on a vertical truncated circular cylinder

Wave forces on a vertical truncated circular cylinder in Stokes waves with the wave slopes ranging from 0.06 to 0.24, are measured in a wave tank. The higher harmonic wave forces are compared with the available values from theories of the FNV (Faltisen–Newman–Vinje) model and Varyani solution. The first harmonic horizontal forces measured are much larger than the theoretical values from the FNV model, while the first harmonic vertical forces are well predicted by the Varyani theory. It was also found that the FNV model significantly overpredicts the second harmonic horizontal forces in high frequency waves, but under predicts the third harmonic forces. The differences between the actual measurement and the theory, in the second and third harmonic horizontal forces, become smaller at low wave frequencies as the wave slope increases. In addition, the transverse instabilities in the incoming waves with high wave slope were observed, which is due to the nonlinear modulation. Measurements were, thus, carried out before the instability occurred.     

水库鱼产力评价标准与模式的研究

本文在总结国内外已有成果的基础上,运用专家系统和灰色统计方法,初步建立了一套水库鱼产力评价的指标体系、指标权重体系和指标标准体系,首次给出了比较系统和具有普适意义的评价方案;并运用模糊数学方法建立了综合定量评价模式,从而在很大程度上解决了以往评价中由于多指标重迭、交错造成的难判、误判问题,为水库渔业资源的合理开发利用提供了比较科学的依据。