基于高通量测序的表层水体纤毛虫多样性评估方法比较与优化

基于扩增子的高通量测序技术广泛应用于微型生物多样性的研究, 不同的测序手段和数据分析流程, 影响着微型生物多样性与群落结构的分析结果。本研究以易于形态鉴定的砂壳纤毛虫作为研究对象, 比较DNA测序、RNA测序和形态学方法检获的多样性和物种组成等, 探究序列分析流程中关键步骤: 嵌合体处理、可操作分类单元分析方法选择、合并相似分类单元以及去除稀有类群等对多样性结果的影响。研究结果显示无论基于DNA还是RNA的分子手段与形态学方法检获的主要物种基本一致, 与DNA测序相比, RNA测序检获的物种数少, 但差异不显著。基于97%以上相似度聚类和单核苷酸变异所得群落结构相似, 无显著差异; 且所有分析方法都能在一定程度上反映出自然界中不同类群的相对丰度。相较于单核苷酸变异和其他相似度阈值, 99%相似度下聚类所得多样性更为接近形态学结果。去除嵌合体和稀有类群(去除阈值: DNA测序0.05%; RNA测序0.07%), 可明显改善分子多样性虚高的问题。本研究为纤毛虫等真核微生物的分子多样性研究提供了科学的数据分析流程, 对未来真核微生物多样性研究具有指导意义。     

Vertical structures of PM10 and PM 2.5 and their dynamical character in low atmosphere in Beijing urban areas

The vertical structures and their dynamical character of PM2.5 and PM10 over Beijing urban areas are revealed using the 1 min mean continuous mass concentration data of PM2.5 and PM10 at 8, 100, and 320 m heights of the meteorological observation tower of 325 m at Institute of Atmospheric Physics, Chinese Academy of Sciences (IAP CAS tower hereafter) on 10―26 August, 2003, as well as the daily mean mass concentration data of PM2.5 and PM10 and the continuous data of CO and NO2 at 8, 100 (low layer), 200 (middle layer), and 320 m (high layer) heights, in combination with the same period meteorological field observation data of the meteorological tower. The vertical distributions of aerosols observed on IAP CAS tower in Beijing can be roughly divided into two patterns: gradually and rapidly decreasing patterns, I.e. The vertical distribution of aerosols in calm weather or on pollution day belongs to the gradually decreasing pattern, while one on clean day or weak cold air day belongs to the rapidly decreasing pattern. The vertical distributive characters of aerosols were closely related with the dynamical/thermal structure and turbulence character of the atmosphere boundary layer. On the clean day, the low layer PM2.5 and PM10 concentrations were close to those at 8 m height, while the concentrations rapidly decreased at the high layer, and their values were only one half of those at 8 m, especially, the concentration of PM2.5 dropped even more. On the clean day, there existed stronger turbulence below 150 m, aerosols were well mixed, but blocked by the more stronger inversion layer aloft, and meanwhile, at various heights, especially in the high layer, the horizontal wind speed was larger, resulting in the rapid decrease of aerosol concentration, I.e. Resulting in the obvious vertical difference of aerosol concentrations between the low and high layers. On the pollution day, the concentrations of PM2.5 and PM10 at the low, middle, and high layers dropped successively by, on average, about 10% for each layer in comparison with those at 8 m height. On pollution days, in company with the low wind speed, there existed two shallow inversion layers in the boundary layer, but aerosols might be, to some extent, mixed below the inversion layer, therefore, on the pollution day the concentrations of PM2.5 and PM10 dropped with height slowly; and the observational results also show that the concentrations at 320 m height were obviously high under SW and SE winds, but at other heights, the concentrations were not correlated with wind directions. The computational results of footprint analysis suggest that this was due to the fact that the 320 m height was impacted by the pollutants transfer of southerly flow from the southern peripheral heavier polluted areas, such as Baoding, and Shijiazhuang of Hebei Province, Tianjin, and Shandong Province, etc., while the low layer was only affected by Beijing's local pollution source. The computational results of power spectra and periods preliminarily reveal that under the condition of calm weather, the periods of PM10 concentration at various heights of the tower were on the order of minutes, while in cases of larger wind speed, the concentrations of PM2.5 and PM10 at 320 m height not only had the short periods of minute-order, but also the longer periods of hour order. Consistent with the conclusion previously drawn by Ding et al., that air pollutants at different heights and at different sites in Beijing had the character of "in-phase" variation, was also observed for the diurnal variation and mean diurnal variation of PM2.5 and PM10 at various heights of the tower in this experiment, again confirming the "in-phase" temporal/spatial distributive character of air pollutants in the urban canopy of Beijing. The gentle double-peak character of the mean diurnal variation of PM2.5 and PM10 was closely related with the evident/similar diurnal variation of turbulent momentum fluxes, sensible heat fluxes, and turbulent kinetic energy at various heights in the urban canopy. Besides, under the condition of calm weather, the concentration of PM2.5 and PM10 declined with height slowly, it was 90% of 8 m concentration at the low layer, a little lesser than 90% at the middle layer, and 80% at the high layer, respectively. Under the condition of weak cold air weather, the concentration remarkably dropped with height, it was 70% of 8 m concentration at the low layer, and 20%―30% at the middle and high layers, especially the concentration of PM2.5 was even lower.     

Uncertainty analysis of CO_2 flux components in subtropical evergreen coniferous plantation

We present an uncertainty analysis of ecological process parameters and CO2 flux components (Reco, NEE and gross ecosystem exchange (GEE)) derived from 3 years’ continuous eddy covariance meas-urements of CO2 fluxes at subtropical evergreen coniferous plantation, Qianyanzhou of ChinaFlux. Daily-differencing approach was used to analyze the random error of CO2 fluxes measurements and bootstrapping method was used to quantify the uncertainties of three CO2 flux components. In addition, we evaluated different ...     

海洋纤维增强热塑性立管极限承载拉力预测方法

海洋新型纤维增强热塑性立管因其可盘卷、耐腐蚀、耐疲劳和轻质化等优点,在深水油气开发中应用前景十分广阔。热塑性立管具有复合材料的各向异性、受力耦合效应及复杂的本构关系,且承受浮体运动和复杂海洋环境载荷,其失效模式尚未明确。针对轴对称载荷作用下纤维增强热塑性立管极限承载力问题,进行热塑性管稳态热传导和热应力的理论推导,求解了稳态温度和应力分布,首次给出了在任意温度载荷作用下管体径向位移的解析解,并直接求解其径向、轴向、环向和剪切应力。采用各向同性层Von Mises和各向异性层最大应力（Max Stress）准则或Tsai-Hill准则判定热塑性管的失效,基于应力分布、失效准则和二分法计算了热塑性管的极限载荷。温度载荷、纤维铺设角度和径厚比对管道的应力分布影响显著。不同温度载荷会改变失效指数沿径向的变化趋势,增大轴向拉力将增大热塑性管的失效指数,选用不同的失效准则在管体失效判定上存在一定的差异。热塑性管温度越低、纤维铺设角越小及径厚比越大,管道对轴向拉伸载荷的承载能力越强。     

爆炸液化场地中浅埋钢筋混凝土结构动力响应的现场试验研究

饱和砂土地基在爆炸荷载作用下会发生液化,地基上的结构物将受到爆炸荷载及地基液化的双重作用,从而产生不均匀沉降和破坏性变形。基于大型现场爆炸液化试验,对场地上钢筋混凝土(RC)结构的动力响应和地基液化后RC结构的变形进行了分析研究。结果表明:液化场地中浅埋RC结构产生了明显的不均匀沉降,且最大沉降量达到结构高度的10%,结构差异沉降达到最大沉降量的1/5,结构沉降变形在液化后15 h时基本稳定;RC结构表面未产生明显的裂缝,动态拉、压应变均在400??以内,不会对结构造成显著破坏;结构动力响应表现为柱侧加速度峰值明显大于梁侧,但柱侧动力稳定所需时间较梁侧短,即柱承受了更大的瞬时冲击力且其抵抗瞬时冲击力的能力更强。研究结果可以为在可液化地基中的浅埋RC结构稳定设计等工程情况提供参考。     

Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China （LCDV-cn） genome

An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene. Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309)     

Laboratory study of deep-water breaking waves

In an attempt to elucidate the mechanics of deep-water wave breaking, a variety of breaking waves, including spilling and plunging waves, of different length scales and geometries was studied. The waves were generated through wave-wave interactions using wave packets with constant-steepness components, constant-amplitude components, and also components following the Pierson-Moskowitz distribution. Wave steepening prior to breaking were found to cause an increase in the high frequency spectral slope of the wave spectrum. The slopes were correlated to the type of breaking and the intensity of the breaking. The energy loss through breaking varied with the spectral characteristics of the wave packet. On the other hand, it was also noted that, irrespective of the wave packet, the losses were from the higher frequency end of the first harmonics.     

岩石变形破坏的熵突变过程与破坏判据

在岩石的变形破坏过程中,当进入不稳定的破裂发展阶段之后,系统不断调整结构抵抗外力的扰动,裂纹向局域集中的有序方向发展,应变能不断耗散,并以应变能的耗散为媒介,使系统与外扰动所追加的负熵流产生能量流通,也使系统熵值和系统的维数逐次降低,这一阶段岩石系统远离平衡态,岩石的破坏是系统熵的突变过程。基于这一认识,并在验证应变能分布与结构块度分布模式的一致性的基础上,推导出包含了结构因素和能量分布的熵表达式;对熵表达式进行平衡分析获得局部突变的分岔集,得到了岩石局部破坏的熵折迭突变破坏准则;同时,探讨了熵表达式所表征的结构有序度的尖点突变性,解出岩石系统的分岔集,这个分岔集就是岩石系统熵突变的整体破坏准则。     

Estimated mass and stiffness matrices of shear building from modal test data

A method that estimates mass and stiffness matrices of shear building from modal test data is presented in this paper. The method depends on only measurable points that are less in number than the total structural degrees of freedom, and on the first two orders of structural mode measured. So it is applicable to most of the general test. Based on this method modal data of unmeasurable points are estimated, then global mass and stiffness matrices of structure are obtained by using the first two orders of modal data. Taking advantage of iteration the optimum global mass and stiffness matrices are gained. Finally, an example is studied in this paper. Its result shows that this method is reliable. © 1998 John Wiley & Sons, Ltd.