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141.
固定化鲅鱼乙酰胆碱酯酶的制备及部分性质测定 总被引:3,自引:0,他引:3
酶的固定化是酶传感器制备过程中重要的1个环节.研究采用直接共价法固定鲅鱼乙酰胆碱酯酶(AChE).制备方法为:0.1g CNBr活化的Sepharose 4B凝胶用1mmol/L的HCl充分溶胀后,与活力为10U的AChE溶液混合,于4℃下150r/min振荡8h.所制备的固定化酶活力回收率较高(96%),对pH值和温度变化的适应能力均优于非固定化酶;在3个月保存期内,前者的活力损失13%,而后者的活力则下降89%.这说明固定过程能够大大提高AChE的抗逆性和保持酶活力的稳定,有利于酶传感器的制备. 相似文献
142.
143.
Lodolo Emanuele Coren Franco Schreider Anatoly A. Ceccone Giulio 《Marine Geophysical Researches》1997,19(5):439-450
The southwestern part of the Scotia Sea, at the corner of the Shackleton Fracture Zone with the South Scotia Ridge has been investigated, combining marine magnetic profiles, multichannel seismic reflection data, and satellite-derived gravity anomaly data. From the integrated analysis of data, we identified the presence of the oldest part of the crust in this sector, which tentative age is older than anomaly C10 (28.7 Ma). The area is surrounded by structural features clearly imaged by seismic data, which correspond to gravity lows in the satellite-derived map, and presents a rhomboid-shaped geometry. Along its southern boundary, structural features related to convergence and possible incipient subduction beneath the continental South Scotia Ridge have been evidenced from the seismic profile. We interpret this area, now located at the edge of the south-western Scotia Sea, as a relict of ocean-like crust formed during an earlier, possibly diffuse and disorganized episode of spreading at the first onset of the Drake Passage opening. The successive episode of organized seafloor spreading responsible for the opening of the Drake Passage that definitively separated southern South America from the Antarctic Peninsula, instigated ridge-push forces that can account for the subduction-related structures found along the western part of the South Scotia Ridge. This seafloor accretion phase occurred from 27 to about 10 Ma, when spreading stopped in the western Scotia Sea Ridge, as resulted from the identification of the marine magnetic anomalies. 相似文献
144.
顶空气相色谱法测定海水二甲基硫和浮游植物细胞二甲基硫丙酸的研究 总被引:12,自引:2,他引:12
建立了顶空GC/FPD测定海水中二甲基硫(DMS)和浮游植物细胞中二甲基硫丙酸(DMSP)的方法,并研究盐度、温度、气液相比DMS诸因素对DMS顶空灵敏度的影响。该法对DMS测定的相对标准偏差均小于6%,平均回收率为106%,最低检出限为20ng/L。细胞DMSP先经碱作用转化为DMS,在50℃下作用时间不少于6h,峰高与浓度的双对数线性相关系数大于0.99。对1994年冬、1995年夏采自胶州湾 相似文献
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对单芯电缆传输这种通讯技术的原理进行了阐述;分析提出了部分电路、部件的技术指标;作为一种数据传输的手段,对单芯电缆传输技术在海洋专用CTD中的应用进行了相关论述. 相似文献
148.
Riccardo?Geletti Emanuele?LodoloEmail author Anatoly?A.?Schreider Alina?Polonia 《Marine Geophysical Researches》2005,26(1):17-28
The structural framework of the southern part of the Shackleton Fracture Zone has been investigated through the analysis of
a 130-km-long multichannel seismic reflection profile acquired orthogonally to the fracture zone near 60° S. The Shackleton
Fracture Zone is a 800-km-long, mostly rectilinear and pronounced bathymetric lineation joining the westernmost South Scotia
Ridge to southern South America south of Cape Horn, separating the western Scotia Sea plate from the Antarctic plate. Conventional
processing applied to the seismic data outlines the main structures of the Shackleton Fracture Zone, but only the use of enhanced
techniques, such as accurate velocity analyses and pre-stack depth migration, provides a good definition of the acoustic basement
and the architecture of the sedimentary sequences. In particular, a strong and mostly continuous reflector found at about
8.0 s two-way traveltime is very clear across the entire section and is interpreted as the Moho discontinuity. Data show a
complex system of troughs developed along the eastern flank of the crustal ridge, containing tilted and rotated blocks, and
the presence of a prominent listric normal fault developed within the oceanic crust. Positive flower structures developed
within the oceanic basement indicate strike-slip tectonism and partial reactivation of pre-existing faults. Present-day tectonic
activity is found mostly in correspondence to the relief, whereas fault-induced deformation is negligible across the entire
trough system. This indicates that the E–W-directed stress regime present in the Drake Passage region is mainly dissipated
along a narrow zone within the Shackleton Ridge axis. A reappraisal of all available magnetic anomaly identifications in the
western Scotia Sea and in the former Phoenix plate, in conjunction with new magnetic profiles acquired to the east of the
Shackleton Fracture Zone off the Tierra del Fuego continental margin, has allowed us to propose a simple reconstruction of
Shackleton Fracture Zone development in the general context of the Drake Passage opening. 相似文献
149.
对虾养殖生态系中有机碳的初步研究本研究由 总被引:1,自引:0,他引:1
对于围隔养殖对虾生态系中有机碳的变动进行了研究 ,结果表明 :溶解有机碳 (DOC)含量波动在 5 .2 99~ 13.39mg/ L之间 ,平均为 8.5 3mg/ L± 2 .2 5 mg/ L;颗粒有机碳 (POC)含量波动在0 .6 5~ 6 .6 3mg/ L之间 ,平均为 3.2 5 mg/ L± 1.76 mg/ L;总有机碳 (TOC)含量波动在 6 .92~ 2 0 .0 2mg/ L之间 ,平均为 11.78mg/ L± 3.82 mg/ L ;其中 DOC∶ POC∶ TOC为 0 .72∶ 0 .2 8∶ 1。各种有机碳组分的含量明显高于自然海水中各种有机碳的含量 ;各种有机碳组分的含量在养殖期间总体上呈上升的趋势 ;其变化与 DCOD的变化呈显著线性正相关的关系。 相似文献
150.