象山港养殖大黄鱼寄生新贝尼登虫成虫形态学和28S rDNA,ITS1分子鉴定

贝尼登类单殖吸虫是象山港海水养殖鱼类中一类危害严重的寄生虫。应用PCR扩增及DNA序列分析的分子生物学手段,并结合对成虫的形态学分析,对象山港养殖大黄鱼体表寄生的贝尼登类单殖吸虫(记作XSp)进行了种类鉴定,结果表明XSp从形态特征上属于新贝尼登虫属,与梅氏新贝尼登虫高度相似。扩增得到XSp的28S rDNA和ITS1序列长度分别为393和427 bp,与梅氏新贝尼登虫和鱾新贝尼登虫的5个28S rDNA 序列、2个ITS1序列的比对分析显示相似性除1个为97.4%外其余均大于99%,提示XSp与这几个鱾新贝尼登虫和梅氏新贝尼登虫为种内关系,而XSp与贝尼登虫的3个28S rDNA 和1个ITS1序列相似性分别为84.3%~89.5%和60.2%。系统进化树显示该吸虫与梅氏新贝尼登虫和鱾新贝尼登虫形成一个紧密的簇,而与贝尼登虫亲缘关系较远。根据普通生物学和序列特征分析,将XSp定种为梅氏新贝尼登虫,并且支持Whittington和Horton (1996)提出的梅氏新贝尼登虫和鱾新贝尼登虫为同种异名的分类观点。     

1997–1998 El Niño off Peru: A numerical study

An eddy-resolving numerical simulation for the Peru–Chile system between 1993 and 2000 is analyzed, mainly for the 1997–1998 El Niño. Atmospheric and lateral oceanic forcings are realistic and contain a wide range of scales from days to interannual. The solution is validated against altimetric observations and the few in situ observations available. The simulated 1997–1998 El Niño closely resembles the real 1997–1998 El Niño in its time sequence of events. The two well-marked, sea-level peaks in May–June and November–December 1997 are reproduced with amplitudes close to those observed. Other sub-periods of the El Niño seem to be captured adequately. Simple dynamical analyses are performed to explain the 1997–1998 evolution of the upwelling in the model. The intensity of the upwelling appears to be determined by an interplay between alongshore, poleward advection (related to coastal trapped waves) and wind intensity, but also by the cross-shore geostrophic flow and distribution of the water masses on a scale of 1000 km or more (involving Rossby waves westward propagation and advection from equatorial currents). In particular, the delay of upwelling recovery until fall 1998 (i.e., well after the second El Niño peak) is partly due to the persistent advection of offshore stratified water toward the coast of Peru. Altimetry data suggest that these interpretations of the numerical solution also apply to the real ocean.     

Trophic modeling of the Northern Humboldt Current Ecosystem, Part II: Elucidating ecosystem dynamics from 1995 to 2004 with a focus on the impact of ENSO

The Northern Humboldt Current Ecosystem is one of the most productive in the world in terms of fish production. Its location near to the equator permits strong upwelling under relatively low winds, thus creating optimal conditions for the development of plankton communities. These communities ultimately support abundant populations of grazing fish such as the Peruvian anchoveta, Engraulis ringens. The ecosystem is also subject to strong inter-annual environmental variability associated with the El Niño Southern Oscillation (ENSO), which has major effects on nutrient structure, primary production, and higher trophic levels. Here our objective is to model the contributions of several external drivers (i.e. reconstructed phytoplankton changes, fish immigration, and fishing rate) and internal control mechanisms (i.e. predator-prey) to ecosystem dynamics over an ENSO cycle. Steady-state models and time-series data from the Instituto del Mar del Perú (IMARPE) from 1995 to 2004 provide the base data for simulations conducted with the program Ecopath with Ecosim. In simulations all three external drivers contribute to ecosystem dynamics. Changes in phytoplankton quantity and composition (i.e. contribution of diatoms and dino- and silicoflagellates), as affected by upwelling intensity, were important in dynamics of the El Niño of 1997–98 and the subsequent 3 years. The expansion and immigration of mesopelagic fish populations during El Niño was important for dynamics in following years. Fishing rate changes were the most important of the three external drivers tested, helping to explain observed dynamics throughout the modeled period, and particularly during the post-El Niño period. Internal control settings show a mix of predator–prey control settings; however a “wasp-waist” control of the ecosystem by small pelagic fish is not supported.     

Growth inhibition and oxidative stress in two species of marine diatoms exposed to 1-phenylethanol

1-phenylethanol (1-PEA) is a flavor extensively used in the production of cosmetics, beverages, and food. The release of 1-PEA into coastal environments has aroused great concern. However, its potential effects on marine organisms are still unknown. In order to provide a better understanding of the ecological risks of 1-PEA in marine environments, this study determined the toxic effects of 1-PEA on two marine diatoms (Phaeodactylum tricornutum and Skeletonema costatum). The diatoms were grown in culture medium containing different concentrations of 1-PEA for 96 h. The contents of chlorophyll a, chlorophyll c, glutathione (GSH), malondialdehyde (MDA), and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), were measured at the end of the exposure period. 1-PEA was shown to significantly inhibit the growth of diatoms, with 96-h EC50 values of 257.14 mg/Land 126.46 mg/L in P. tricornutum and S. costatum, respectively. In P. tricornutum, the levels of SOD, CAT, GPx, GSH, and MDA were stimulated only when 1-PEA concentrations were close to or greater than the 96-h EC50 value. However, in S. costatum, the activities of SOD and CAT, and the syntheses of two chlorophylls were inhibited even at an exposure concentration below the 96-h EC50 value. Taken together, these findings indicate a potential ecological risk by discharging 1-PEA into coastal areas and its species-specific toxic effects on marine organisms.     

基于多源数据的山东省围填海造地特点及发展趋势研究

文章基于文献资料分析、官方数据汇总、国产高分辨率资源卫星遥感影像信息提取以及发展趋势研判等方法,对改革开放以来山东省全海域范围内围填海造地的历史发展、现状特征、发展趋势及其对生态环境的影响进行了分析和总结,并提出相关的政策建议。研究表明:改革开放以来,山东省围填海造地累计已超过4.58万hm~2。21世纪以来围填海造地进入快速增长时期,尤其是2010年以来,全省围填海造地又掀起了一次新的热潮,其面积和速度都远远超过了历史水平。鉴于围填海造地对环境和生态的巨大影响,文章提出了加强管理、严格环境影响评价、引入生态环境补偿机制以及建立分级填海评估制度和年度监测机制等建议。     

基于CO1基因的渔山列岛厚壳贻贝遗传资源评估

基于线粒体CO1基因的序列,对渔山列岛,大连獐子岛,南麂列岛,山东南隍城乡,舟山嵊泗5个野生群体的厚壳贻贝进行遗传多样性和遗传结构的分析。结果表明:在mt DNACO1基因同源片段上共检测到了39个多态位点,其中39个简约信息位点,无单突变位点,构成40个单倍型,群体遗传多样性水平为:渔山山东大连舟山南麂;然而单倍型多样性却是山东渔山南麂大连舟山。分子进化树和单倍型网络关系图构建结果显示:5个群体之间分为3个单倍型类群(A、B、C),其中A类群含15个单倍型,B类群含23个单倍型,C类群含2个单倍型(均为渔山列岛的单倍型),其中渔山列岛的单倍型在上述三个类群中都有分布。3个单倍型类群间的遗传距离为0.0099~0.0268。渔山列岛群体内遗传分化较为显著,其他4个群体间未检测到显著的遗传分化。     

基于HJ-1A/1B的2014年黄海海域浒苔灾害时空分布

自2007年以来, 黄海海域每年的5月初～8月中下旬浒苔(Ulva prolifera)会周期性地暴发与消亡,导致海洋生态环境被破坏以及经济损失。利用2014 年的HJ-1A/1B遥感影像, 利用神经网络监督分类及RULE规则影像重分类动态阈值法, 对2014年的浒苔的漂移路径、各时期影响的海域面积、分布面积以及暴发高峰期的最大面积进行了动态监测。结果表明, 2014年浒苔持续时间为101 d, 5月中旬开始在江苏省盐城市近海出现零星斑点, 分布面积为2.299 km², 影响面积为1 744.799 km²; 6月初到6月中旬浒苔广泛分布于黄海海域, 分布面积扩大至1 367.145 km², 达到当年的峰值; 从6月下旬开始, 浒苔进入衰退期, 浒苔分布面积、相对聚集密度均急剧缩小, 但影响面积的峰值出现在该时期; 8月初消亡于青岛附近海岸, 8月20日遥感影像已难以监测到浒苔的存在。2014年黄海海域浒苔经过了“出现—发展—暴发—衰退—消亡”5个发展阶段。     

荔湾3-1外输海底管道中落管抛石技术

落管抛石是进行深水抛石的一种工程技术,可作为保证管道稳定性、处理悬跨、抑制隆起屈曲和进行跨越支撑的工程解决方案。系统研究了抛石在波流作用下的稳定性,落石对管道冲击的影响以及施工技术。针对南海环境条件下的抛石稳定理论进行了修正;分析了三层聚丙烯涂层管道在落石冲击下的可接受抛石粒径;提出同时结合石块稳定性结果和管道抗冲击性能要求下的石料分级的方法;最后,对该技术在荔湾3-1外输海底管道的工程应用进行详细阐述。     

基于HJ-1A高光谱的黄河口碱蓬和柽柳盖度反演模型研究

碱蓬和柽柳是黄河口湿地典型的盐生植物类型,是多种保护珍禽的主要栖息地,具有景观尺度较小、分布广且多混生的特点。应用覆盖黄河口北部潮滩的HJ-1A高光谱遥感影像,基于现场测量的端元光谱和从遥感影像中使用顺序最大角凸锥法(SAMCC)自动提取的端元光谱,应用线性光谱分解法(LSU)、正交子空间投影法(OSP)、匹配滤波法(MF)、最小能量约束法(CEM)和自适应一致估计法(ACE)5种不同光谱解混方法进行混合像元光谱解混,对比两种方法得到的端元光谱分别对碱蓬和柽柳盖度的反演能力,并给出相应的反演模型。结果显示:(1)现场测量端元光谱取得了较好的碱蓬和柽柳盖度反演结果,其中应用LSU方法的光谱解混结果与现场测量盖度的决定系数对于碱蓬和柽柳分别达到了0.88和0.95;(2)两种端元获取方式的光谱解混结果中,LSU和OSP方法均获得了较高的相关性,ACE解混方法的相关性都最低;(3)SAMCC方法提取端元光谱对柽柳的分解结果与现场测量盖度的相关性远高于碱蓬。     

Laboratory Study for Influence of Clay Content (CC) on Wave-Induced Liquefaction in Marine Sediments

In this artice, the influence of clay content on the wave-induced liquefaction in marine sediments was reported. The one-dimensional (1-D) equipment was setup with a vertical cylinder and 1.8–m–thick clayey sandy deposit and 0.2–m–thick water above the deposit. Unlike the previous experimental study for a single soil layer, this study used sand-kaolin mixtures, sand-illite mixtures, and sand-bentonite mixtures as the experimental samples. A series of experiments with 3,000 wave cycles in each test were conducted under numerous wave and soil conditions, which allowed us to examine the influence of clay content (CC) on wave-induced liquefaction in marine sediments. The experimental results showed that the clayey sandy deposit will become prone to liquefaction with the increase of CC when CC is less than a critical value, which depends on the type of clay. However, when CC is greater than the critical value, liquefaction depth will decrease as CC increases. Furthermore, when the CC value reaches a certain level, liquefaction will not occur. For example, no liquefaction occurs when CC ≥ 33% for both kaolin-sand and illite-sand mixtures and CC ≥ 16.36% for bentonite-sand mixtures.