Optimization of preparative separation and purification of total polyphenols from Sargassum tenerrimum by column chromatography

Polyphenols from the ethanol extracts of Sargassum tenerrimum (ST) with potent antiallergic effects were studied to optimize separation process through column chromatography. The adsorption and desorption characteristics of three widely used adsorbents: macroporous resin, silica gel, and polyvinylpolypyrrolidone (PVPP), were critically evaluated respectively and studied for the optimization of preparative separation of polyphenols. Static operations on these adsorbents showed that macroporous resin had the best adsorption and desorption capability among the three adsorbents. Dynamic adsorption and desorption with macroporous resin packed column were also conducted to optimize the parameters such as: with the optimal values shown in brackets, the concentration of extract solution (4 times diluted), pH value (6–7), adsorption speed (3 BV h⁻¹, bed volumes/per hour), concentration of ethanol (80%), elution speed (3 BV h⁻¹) and elution volume (7 BV). The chromatographic process so optimized gave a purity of 62.43% from the crude polyphenols, providing a promising basis for large scale preparation of bioactive polyphenols upon further scaling up tests.     

Optimization of Preparative Separation and Purification of Total PolyphenoJs from Sargassum tenerrimum by Column Chromatography

Polyphenols from the ethanol extracts of Sargassum tenerrimum （ST） with potent antiallergic effects were studied to optimize separation process through column chromatography. The adsorption and desorption characteristics of three widely used adsorbents： macroporous resin, silica gel, and polyvinylpolypyrrolidone （PVPP）, were critically evaluated respectively and studied for the optimization of preparative separation of polyphenols. Static operations on these adsorbents showed that macroporous resin had the best adsorption and desorption capability among the three adsorbents. Dynamic adsorption and desorption with macroporous resin packed column were also conducted to optimize the parameters such as： with the optimal values shown in brackets, the concen- tration of extract solution （4 times diluted）, pH value （6-7）, adsorption speed （3BVh-1, bed volumes/per hour）, concentration of ethanol （80%）, elution speed （3 BV ht） and elution volume （7 BV）. The chromatographic process so optimized gave a purity of 62.43% from the crude polyphenols, providing a promising basis for large scale preparation of bioactive polyphenols upon further scaling up tests.     

马尾藻的营养组成及其袋泡茶饮料的试制

对马尾藻进行营养成分的分析，结果表明马尾藻蛋白质的氨基酸组成均衡，氨基酸价为82，且富含无机质等。无机质中除了钙、钾和钠含量高外，碘含量达820mg/kg，是研制含碘食品和饮料的理想原料。马尾藻经加工制得的袋泡茶符合国家卫生标准，袋泡茶（2g/袋）经沸水冲泡后的碘浸出量为0.51mg/袋，可防治甲状腺机能减退病，即碘缺乏病。     

Fluoride contents in groundwaters and the main consumed foods (dates and tea) in Southern Algeria region

In the Algeria South, the dental fluorosis is a “silent” epidemic spreading within the populations. The present study was aimed to determine the fluoride content in water, dates and tea that are widely consumed. The results reveal that 35% of water wells had excessive fluoride levels (>1.5 mg/L). The eastern areas where dental decay is the most widespread pathology present the highest contents. Dates, tea and water are considerable sources of fluoride, with a daily contribution of 10, 20 and up to 70%, respectively. For these three sources, the daily intake of fluoride ingested by an adult exceeds the proposed safe threshold of 0.05–0.07 mg/kg day.     

Anti-oxidant and anti-inflammatory activities of ultrasonicassistant extracted polyphenol-rich compounds from Sargassum muticum

Polypehnol is an important,potentially bioactive component of Sargassum muticum.In this study,ultrasonic assisted extraction of polyphenol-rich substances was performed using a 38%ethanol solution at a solid:liquid ratio of 1:30 at 68℃ for 32min,determined by single-factor and response surface methodology(RSM)optimization.The content of polyphenol was 5.66mg/g in the crude extract.Further extraction showed that the polyphenol mainly distributed in ethyl acetate(SKEE)and water phases(SKEW).The anti-oxidation test by electron spin resonance(ESR)spectrum showed that the SKEE had the strongest scavenging activity on DPPH(1,1-diphenyl-2-picrylhydrazyl)and alkyl radicals.SKEE was shown noncytotoxic but could inhibit the generation of cellular ROS,showing protective effects in H2O2 and AAPHinduced Vero cells and UV-B irradiated HaCaT cells.SKEE also signifi cantly inhibited the release of NO of LPS-induced RAW 264.7 cells.Therefore,the polyphenol-rich extracts in ethanol and ethyl acetate showed excellent anti-oxidant and anti-infl ammatory activities,which is beneficial to the development of high-value bio-substances.     

海带多酚的产生与抗褐藻酸降解菌感染的相关性

利用生物化学的方法探讨了褐藻酸降解菌感染过程中海带体内多酚含量的变化 ,并分析了多酚含量的高低与海带对褐藻酸降解菌感染抗性的相关性。研究结果表明 :褐藻酸降解菌感染下海带体内多酚含量急剧上升 ,并且这种变化具有普遍性 ;多酚含量的急剧上升只发生在褐藻酸降解菌感染的初期阶段 ;海带体内多酚含量的高低与海带对褐藻酸降解菌的抗性具有明显的相关性。表明多酚类化合物在海带抵抗褐藻酸降解菌的感染中起重要作用     

福建不同地区人工引种无瓣海桑(Sonneratia apetala)生理生态研究

采用乙醇提取法、纳氏比色法、钼锑抗显色法及F-C法分别研究了人工引种于福建省漳浦、集美、泉港、连江无瓣海桑(Sonneratia apetala)成熟叶片的生理指标:叶绿素、氮、磷及总酚含量。结果表明,4个地区叶片叶绿素a含量在(1.44±0.54)—(2.52±0.94)mg/g之间波动,叶绿素b含量范围在(0.54±0.02)—(0.94±0.11)mg/g之间,叶绿素a/b随着纬度的升高有增大趋势,表明随纬度升高无瓣海桑对蓝紫光需求比例增大;各地点无瓣海桑氮、磷含量均无显著差异,且N:P比值均小于14,表明均存在N限制;总酚含量随着纬度的升高而逐步升高,可能与无瓣海桑生长条件随纬度的上升而受到外界环境胁迫增大有关。     

湖北省茶树种植气候区划

利用湖北省74个气象台站1971-2005年的气候资料,在地理信息系统(GIS)平台的支持下,结合茶树的生态学特性,运用模糊综合评价法对湖北茶树种植实现基于1 km×1 km格网的气候适宜性区划,分为最适宜种植区、适宜种植区、较适宜区和不适宜区.结果显示:鄂西南、鄂东南地区以及鄂东北部分地区为最适宜茶树种植区,面积约占湖北全省的27.1%;鄂西北及鄂中部分地区为不适宜区,主要受到年降水量和年极端最低气温的限制,面积约占全省的19.8%;其他地区则处于适宜和次适宜种植区.将区划结果与湖北历年茶叶产量数据进行比对,发现两者吻合度较高,即从茶树气候适宜性角度实现的精细化评价结果对指导湖北省的茶树种植规划具有一定的应用价值.