草鱼出血病病毒VP7蛋白基因的人工合成与原核表达

根据GenBank已发表的GCRVVP7蛋白基因序列,设计合成2条特异性引物和19条搭桥引物,通过搭桥PCR人工合成带有3处变异碱基的GCRVVP7蛋白基因整个编码框,经过酶切、连接、PCR鉴定和测序鉴定克隆到原核表达载体pColdTF中,并转化大肠杆菌BL21(DE3),进行IPTG诱导表达和表达产物的SDS-PAGE、Western-blotting分析、纯化以及反应原性的iELISA检测。结果表明,成功人工合成了长831bp的GCRV VP7蛋白基因编码框和构建了VP7蛋白基因的重组质粒pCold TF-VP7,pColdTF-VP7转化菌经IPTG(1mmol/L)诱导3—4h即可获得特异性蛋白VP7重组蛋白的高效表达,VP7重组蛋白相对分子量大小约为73.9ku,与预期大小相符,且与鼠抗GCRV血清有良好反应原性。     

A new chronological framework for Middle and Upper Pleistocene landscape evolution in the Sussex/Hampshire Coastal Corridor, UK

The unique Middle and Late Pleistocene sedimentary record preserved along the Sussex/Hampshire Coastal Corridor between Romsey and Brighton contains a wealth of deposits including highstand marine sediments associated with a variety of different aged beaches, fluvial sediments associated with rivers crossing the coastal plain and cold stage deposits accumulating above the marine and fluvial sediments. Although quarrying activity has been extensive across much of the area it has been undertaken in flooded workings due to the high level of the watertable. Consequently little is known in detail about the sequences except where they outcrop on the foreshore around the coast. This paper examines recent work from the lower coastal plain using a multi-disciplinary approach these deposits to elucidate the age of the sequences and their associated environments of deposition.OSL dates from two of the beaches, the Aldingbourne and Brighton/Norton Beaches, place both within MIS 7. Although these OSL dates cannot differentiate between sub-stages within MIS 7, coupling these results with inferences from local geography, lithology and contained microfossils it is clear that the beaches belong to two different phases within MIS 7. These two beaches are clearly divided by a major phase of erosion and downcutting associated with a fall in sea-level. Fluvial sediments from Solent Terrace 2 and Arun Terrace 4 also date within MIS 7 and are tentatively ascribed to the downcutting event between the beaches. Together this information allows us to propose, for the first time, a robust independently dated framework for the lower parts of the coastal plain integrating for the first time the marine and terrestrial record.     

210Pb and 7Be as Tracers for Aerosol Transfers at Center Guizhou，China：Ⅰ．The Explanation by Weekly Interval

It′s essential to measure air concentrations and depositional fluxes of environmental radionuclides in different regions for modeling global contamination transfer and validation, tracing deposition process and dating, referencing watershed erosion trace and assessing natural radiation of ecosystem. This study introduces the research background and methods and explains weekly variations of ²¹⁰Pb and ⁷Be concentrations in surface air and precipitation at Mt. Guanfeng/Guiyang since 2001. The results indicate that ²¹⁰Pb and ⁷Be concentrations in surface air fluctuate periodically at 2~6 week interval because of the meteorological condition changes. The annual trend in ²¹⁰Pb air concentration is overall influenced by air temperature variation and periodical rainfall. ⁷Be air concentration varies up and down regualarly. The synchronization of low concentrations for both ²¹⁰Pb and ⁷Be in surface air and high ratio (²¹⁰Pb/⁷Be ≥ 0.8) indicate oceanic poor ²¹⁰Pb-⁷Be air mass invasion. The synchronization of high concentrations of ²¹⁰Pb and ⁷Be in surface air and low ratio (²¹⁰Pb/⁷Be ≤ 0.7) implies rich ⁷Be air mass invasion from altostratus atmosphere. Weekly concentrations of ²¹⁰Pb and ⁷Be in precipitation and the K_d coefficients are high in late Autumn to Spring, and low in late Spring to Autumn. Although ²¹⁰Pb and ⁷Be concentrations vary seasonally, their weekly concentrations in precipitation increase with that of air concentration. The ²¹⁰Pb and ⁷Be weekly concentrations for seasonal division is about 0.2 Bq/L and 1 Bq/L, respectively. However, the K_d coefficient decreases when ²¹⁰Pb and ⁷Be air concentrations increase. Deposition of ²¹⁰Pb and ⁷Be with precipitation mainly take place in Spring and Summer; The weekly fallout of ²¹⁰Pb and ⁷Be increases with increasing precipitation; the increase of ⁷Be is triple of that of ²¹⁰Pb and 2.5 when precipitation nears to zero. The weekly-high fallout of ²¹⁰Pb and ⁷Be with precipitation corresponds with the dramatic decrease of their air concentrations within or vicinal weeks. The ratio of ²¹⁰Pb to ⁷Be in rainfall is overall ≤ 0.5 and varies synchronously with the ratio in surface air, indicating that the two radionuclides are cleaned by precipitation from air at close speed. In the extreme freezing disaster in 2008, both ²¹⁰Pb and ⁷Be air concentrations were low and coupling with coexist of low concentration of aerosol and high nuclides activities. Weekly concentration of ⁷Be in surface air at Mt. Guanfeng demonstrates the anticipated concentration level in the low-latitude and high-elevation region; the short-term fluctuations of ⁷Be concentration indicated the hysteresis was influcenced by the strong air current submersion and the low-elevation air mass rising differently at Mt. Guanfeng and Mt. Waliguan.      

Effects of substratum type on fish assemblages in shallow areas of a tropical estuary

While there is already a comprehensive understanding of the effects of environmental variables, such as dissolved oxygen, temperature and salinity, on the structure, biomass and metabolism of aquatic biota in estuarine habitats, the effect of sedimentation, a harmful underlying factor, remains unclear. The aim of this study was to assess the differences in fish assemblages along the freshwater to salt water gradient of a large tropical estuary, and to evaluate the effects on them of habitat disturbance associated with shallow water sedimentation in the intertidal areas. Fish and environmental variables were recorded in the upper, middle and lower salinity zones of the estuary at ebb tide in both the dry and wet seasons. Sediment samples associated with different levels of habitat disturbance were analysed using granulometry, and their organic content and dissolved oxygen levels were quantified. Water temperature, salinity, pH and dissolved oxygen levels were also measured. Habitat disturbance was found to be correlated with the density, biomass and richness of fish assemblages. A total of 77 species were recorded, forming two distinct fish assemblages, with the Eleotridae family dominating in the upper zone, and Gerreidae, Gobiidae and Tetraodontidae the most common in the middle and lower estuary. Changes in the structure of fish assemblages, including reductions in density, biomass and richness, were associated with disturbance to natural features, where muddy sediment was replaced by sandy sediment and the quantity of organic matter fell. Atherinella brasiliensis was the species which showed a preference for the disturbed areas in the middle and lower zones, while Dormitator maculatus showed a preference for them in the upper estuary. They may be taken as indicators of habitat disturbance due to shallow sedimentation.     

东亚季风区近地面大气气溶胶中OCPs和PCBs纬度分布：以7Be为参照系

连续一年在北京、青岛、广州以每周3d的时间尺度同步采集近地面大气中气溶胶中7Be的测定数据以及在春、秋季节变换时期我国5个不同纬度城市大气气溶胶中7Be和典型持久性有机污染物（有机氯农药和多氯联苯1的同步观测数据,并对文献上发表的我国其他城市近地面大气中气溶胶中’Be年平均值数据进行了分析。通过对以上数据资料的总结和分析,观察到在东亚季风区近地表大气气溶胶中7Be浓度的年平均值呈现正态分布模式,并且在中纬度北纬40°N附近达到极大值。大气气溶胶中7Be在春、秋季节变换时期我国不同纬度城市的瞬时纬度分布仍呈现正态分布模式,但以30°N为最大值。在秋季大气颗粒相中HCHs和PCBs浓度最大值出现在30°N。气相中PCB-28所占百分比随纬度增高而增大,而颗粒相中PCB-28的纬度变化不大。蒸汽压较低的PCB-180在气相中的浓度基本上不随纬度变化,而颗粒相中PCB-180则基本上集中在纬度36。N左右,表明在东亚季风区大气中挥发性较低的POPs化合物具有某种纬度聚焦作用。以宇宙射线成因核素7Be作为大气环流的参照系,可以得出东亚季风区大气环流可影响持久性有机污染物纬度分布的结论。     

基于定向片模型的SPOT-5遥感影像自检校光束法平差

本文通过建立内方位自检校模型,利用定向片模型对外方位元素进行内插建模,建立了基于定向片模型的自检校光束法平差,研究不同控制点数量和不同定向片间隔对定位精度的影响,并利用河南嵩山地区SPOT-5遥感影像数据对模型进行了实验验证和分析,结果表明,平面精度可以达到10.06m,高程精度可以达到8.84m.     

地下侧积砂坝建筑结构研究及储层评价——以孤东油田七区西Ng52+3砂体为例

分析了在不同沉积环境下侧积砂坝的沉积模式,研究了侧积体的空间组合特征,并利用模式预测的方法建立侧积砂坝的建筑结构模型。研究表明,孤东油田七区西Ng52+3侧积砂坝是小型河流形成的,其侧积体的空间组合模式为水平斜列式,侧积体的宽度为80～240 m,倾角为5°～12°。薄片、扫描电镜分析证实,七区西Ng52+3的成岩作用较弱,原始的粒间孔隙保存完好,储层孔隙度平均为32.3%,渗透率为3 500×10-3 μm2。侧积砂坝内含有高岭石、蒙脱石、伊利石等粘土矿物,使其储层物性变差。     

鄂尔多斯盆地庆城地区延长组7段致密油特征及油源分析

鄂尔多斯盆地三叠系延长组7段(长7段)半深湖—深湖相优质油源岩是油气的重要来源,包括黑色页岩和暗色泥岩,作为盆地重要的供烃源岩一直是研究的热点。为了明确不同烃源岩的贡献及油气来源,通过对庆城地区长7段致密油特征进行分析,对原油进行精细划分,并对源岩有效性进行分析,进一步明确油源对应关系,研究表明,庆城地区长7段黑色页岩和暗色泥岩均对油气成藏有贡献,其中黑色页岩贡献最大,长7₃段暗色泥岩次之,长7₂和长7₁段暗色泥岩贡献较小;原油为低密度、中—低黏度和低凝固点的轻质油。根据生标物特征将原油划分为3类,A类原油具有低C₃₀重排藿烷/C₂₉藿烷、低C₂₉Ts/C₂₉藿烷、低Ts/Tm的特征,由黑色页岩贡献,主要分布于长7₁段;C类原油具有高C₃₀重排藿烷/C₂₉藿烷、高C₂₉Ts/C₂₉藿烷、高Ts/Tm的特征,主要由暗色泥岩贡献,...     

遥感在大兴安岭森林覆盖区地质矿产调查中的应用——以黑龙江洛古河1:5万区域地质矿产调查工作为例

大兴安岭地区森林覆盖严重, 气候严寒, 交通极为不便, 野外有效工作时间短, 给区域地质矿产调查工作增加了难度, 急需遥感手段提高成果质量和效率. 在黑龙江大兴安岭洛古河等4幅1:5万区域地质矿产调查工作中, 利用SPOT7、Landsat7/8、ASTER等多种遥感数据开展地质矿产解译, 进行遥感影像分区, 建立地层、构造和侵入岩解译标志, 提取羟基和铁染蚀变异常, 结合水系沉积物测量成果划分了成矿有利区, 有效降低了地质矿产调查强度, 提高了调查效率, 增强了调查质量. 表明遥感技术在大兴安岭高植被覆盖区地质矿产调查过程中能够取得较好效果.     

鄂尔多斯盆地南部延长组长7油组页岩层系天然裂缝发育特征及主控因素

利用地表露头、岩心、薄片和测井资料,对鄂尔多斯盆地南部长7油层组陆相页岩层系天然裂缝的成因类型、发育特征和主控因素进行研究。按地质成因,鄂尔多斯盆地南部长7油层组页岩层系的天然裂缝可以分为构造裂缝和成岩裂缝两大类,其中构造裂缝主要包括剪切裂缝和张性裂缝;成岩裂缝主要包括层理缝、页理缝和收缩裂缝。不同岩性中的天然裂缝发育情况明显不同,致密砂岩中以构造裂缝为主,层理缝仅在粉砂岩和局部细砂岩中发育;页岩和凝灰岩中页理缝、层理缝和构造裂缝均比较发育。不同类型的天然裂缝主控因素明显不同,构造裂缝的发育主要受岩性和岩石力学层层厚控制;砂岩中层理缝的发育主要受碳质纹层、粘土矿物转化程度等因素控制;凝灰岩中层理缝的发育主要受刚性玻屑脱玻化作用的控制;页岩中页理缝的发育主要受纹层、TOC含量和黄铁矿含量的控制。