RAPD分析中最适样本量和位点数的研究

用25个引物,对40个样本扩增,研究了勒氏笛鲷随机扩增多态性DNA(RAPD)分析中的最适样本量和位点数。结果显示:在样本量达到20且位点数达到70个以上时,遗传距离趋于一个稳定的数值,即在RAPD分析中,样本数应达到20且位点数应达到70才能保证结果的可靠性。     

Chemical dynamics of N-containing ionic species in a boreal forest snowcover during the spring melt period

A study of the changes in the ionic loads of NO, NH, SO and H⁺ in a boreal forest snowpack at Lake Laflamme, Québec was carried out using hydrological and chemical data from field lysimeters. The results showed that depletion of the N-containing species occurs periodically in the snowpack during meltwater discharge. Rain-on-snow events led to in-pack losses of NO and NH at a rate of 130 μeq m^?2 day^?1 and 101·3 μeq m^?2day^?1 respectively. On dry days, however, dry deposition and deposition of organic debris from the canopy resulted in increases of 183·3 μeq m^?2day^?1 for NO and 4·5 μeq m^?2day^?1 for NH in the pack. In contrast, SO₄^2? showed continual in-pack increases due to deposition of 5·0 μeq m^?2day^?1 for wet days and 92·6 μeq m^?2day^?1 for dry days. The depletion of NO and NH is due to microbiological uptake of these nutrients during periods when the free water content of the pack is high. Controlled melts in a laboratory snowmelt simulator containing snow and organic matter from the forest canopy at Lake Laflamme showed losses of NO and NH similar to those observed in the field. As the microbiological uptake proceeds at a rate comparable to that of ionic load increases in the pack by dry deposition, models of the chemical dynamics of snowmelt should take the former into account in any system where organic content of the snowpack is appreciable.     

Spatial statistics of clustered data

Modern spatial statistics techniques are widely used to make predictions for natural processes that are continuously distributed over some convex domain. Implementation of these techniques often relies on the adequate estimation of certain spatial correlation functions such as the covariance and the variogram from the data sets available. This work studies the practical estimation of such spatial correlation functions in the case of clustered data. The coefficient of variation of the dimensionless spatial density of the point pattern of sample locations is suggested as a useful metric for degree of clusteredness of the clustered data set. We show that the common variogram estimator becomes increasingly unreliable with increasing coefficient of variation of the dimensionless spatial density of the point pattern of sample locations. Moreover, we present a modified form of the variogram estimator that incorporates declustering weights, and propose a scheme for estimating the declustering weights based on zones of proximity. Finally, insight is gained in terms of a numerical application of the common and modified methods on piezometric head data collected over an irregular network.Acknowledgments. This work has been supported by grants from the National Institute of Environmental Health Sciences (P42 ES05948-02), the Army Research Office (DAAG55-98-1-0289), and the National Aeronautics and Space Administration (60-00RFQ041). Some of the calculations conducted in support of this work were done on the SGI Origin 2400 at the North Carolina Supercomputing Center, RTP, NC.     

Stable carbon isotope ratios in lake and swamp sediments as a proxy for prehistoric forest clearance and crop cultivation in the Neotropics

Close correspondence between stable carbon isotope ratios ( ¹³ C), pollen, and charcoal profiles in sediment cores from Laguna Zoncho and Machita swamp, Costa Rica, shows that prehistoric forest clearance and crop cultivation can be detected in the stable carbon isotope ratios of total organic carbon ( ¹3C _TOC ). Analyses of δ 13C _TOC complement evidence from pollen, charcoal, and phytoliths and provide a proxy that is sensitive to the intensity and/or proximity to core sites of prehistoric forest clearance and agriculture in watersheds. Stable carbon isotope analyses are particularly useful in situations in which other evidence of forest clearance and agriculture is limited.     

昆明西山森林公园旅游垃圾管理研究

采用了情景模拟、现场观测和访谈等方法研究了昆明西山森林公园旅游垃圾管理中存在的问题,研究发现：（1）游道两侧垃圾散落严重,且多分布于隐蔽区域;（2）公园内存在诸多隐形休息区,同时也是旅游垃圾管理的盲区;（3）环卫设施设计忽视人体工学原理;（4）垃圾分类概念模糊,缺乏规范标准;（5）环境教育意识淡薄,多流于形式。研究发现依据旅游垃圾的二次利用性进行分类、重视环境教育、合理设计和布局环卫设施、科学分类收集和合理分类处理等措施有利于西山森林公园旅游垃圾的有效管理。     

冰雪灾害对吉安市青原区森林资源危害及恢复措施

2008年的低温冻雨冰雪灾害性天气对吉安市青原区林业生产造成了极大损失。以青原区8个乡镇森林为调查对象,调查不同区域、不同海拔、不同坡度、不同林种、不同树种的受灾若干特征,了解各种树种的防御雪灾的能力,提出恢复重建的具体措施,认为需要政府支持、科学经营和加强管护。     

Investigation of fractal distribution law for the trace number of random and grouped fractures in a geological mass

A fractal study method of the number of geological mass fractures is introduced in detail in this paper. Three main aspects of the problem were studied: (1) The random distribution of fractures in a geological mass was in good agreement with the fractal law. The size scale of the studied geological mass ranged from 2400 m to 1 mm for the length of each side, and the geological mass samples were taken from 13 coal areas in China. (2) The geological mass fractures were evidently directional and anisotropic, having originated from tectonic movement. Observation and statistics for the data from the Xuangang, Fenxi and Dongshan coal areas in Shanxi, China, demonstrated that the fracture distribution of each group, classified by the strike of the strata, still follow the fractal law, even though the fractal dimension varies to a certain extent with different strikes. (3) The sedimentary strata containing the coal seams, as a geological mass, underwent almost similar tectonic movements in their geological history. The mechanical experiments on geological mass samples from Fenxi and Jiexiu in Shanxi demonstrated that the fractal dimension of the number of fractures in the same strata is in good power function with the product of strength and elastic modulus. The larger the product of the strength of the elastic modulus is, the larger is the fractal dimension, and vice versa.     

Nematode distribution in cultivated and undisturbed soils of Guinea Savannah and Semi-deciduous Forest zones of Ghana

Climate change affects air temperature, sea levels as well as the soil and its ecosystem. The Guinea Savannah and Semi-deciduous Forest zones of Ghana are characterized by different climatic conditions and vegetative cover. Annual average temperature has been steadily increasing whilst annual total rainfall has been decreasing in both zones, and this has been causing a southward shift of the Savannah into the Forest zone. Soil organisms provide crucial ecosystem services which are required for sustainable agriculture and food production yet crop cultivation disturbs the soil ecosystem. The harsh conditions associated with the Savannah further expose the soil ecosystem to disturbance and loss of biodiversity which threatens food production and security. Soil nematodes are the most abundant animals in the soil and play a central and critical role in the soil food web complex. Studying the nematode community structure gives a reflection of the status of the entire soil ecosystem. Soil samples were taken from cultivated and natural landscapes in the Guinea Savannah and Semi-deciduous Forest agroecological zones to analyse the nematode community. Results from the study showed the Guinea Savannah zone recording warmer soil temperatures, lower organic matter percentage and lower nematode diversity(Genus Richness) as compared to the Semi-deciduous Forest zone. If the Savannah continues to shift southward, the Forest zone soil ecosystem risks disturbance and loss of biodiversity due to the harsh Savannah conditions. Our findings indicate that prevailing crop cultivation practices also disturb soil ecosystem in the two ecological zones which span across West Africa. A disturbed soil ecosystem endangers the future of food production and food security.     

The Fernow Experimental Forest,West Virginia,USA: Insights,datasets, and opportunities

Long-term experimental watershed studies have significantly influenced our global understanding of hydrological processes. The discovery and characterization of how stream water quantity and quality respond to a changing environment (e.g. land-use change, acidic deposition) has only been possible due to the establishment of catchments devoted to long-term study. One such catchment is the Fernow Experimental Forest (FEF) located in the headwaters of the Appalachian Mountains in West Virginia, a region that provides essential freshwater ecosystem services to eastern and mid-western United States communities. Established in 1934, the FEF is among the earliest experimental watershed studies in the Eastern United States that continues to address emergent challenges to forest ecosystems, including climate change and other threats to forest health. This data note describes available data and presents some findings from more than 50 years of hydrologic research at the FEF. During the first few decades, research at the FEF focused on the relationship between forest management and hydrological processes—especially those related to the overall water balance. Later, research included the examination of interactions between hydrology and soil erosion, biogeochemistry, N-saturation, and acid deposition. Hydro-climatologic and water quality datasets from long-term measurements and data from short-duration studies are publicly available to provide new insights and foster collaborations that will continue to advance our understanding of hydrology in forested headwater catchments. As a result of its rich history of research and abundance of long-term data, the FEF is positioned to continue to advance understanding of forest ecosystems in a time of unprecedented change.