盐度对近江牡蛎耗氧率、排氨率、O:N 和吸收率的影响

以半现场流水槽法研究了不同盐度(5、10、15、20、25、30、35)对近江牡蛎(Crassostrea hongkongensis)耗氧率、排氨率、O:N、吸收率的影响,并测定了盐度为20条件下牡蛎耗氧率和排氨率的周日变化.结果表明,近江牡蛎耗氧率、排氨率、O:N先随着盐度的升高而下降,在20左右降到最低,随着盐度继续上升,又升高;而近江牡蛎的吸收率先随着盐度的升高而升高,在20左右达到最高,然后随盐度升高而下降.根据数据得出耗氧率与盐度的拟合方程: y=0.0033x2-0.1161x+1.5523, R2=0.9018;排氨率与盐度:y=0.0001x2-0.0041x+0.0871, R2=0.9889;O:N与盐度:y=0.0016x3-0.0782x2+0.9051x+10.818, R2=0.955;吸收率与盐度: y=-0.0011x2+0.0399x+0.4393.R2=0.9453.一日内,8时、14时、19时、22时4个时间点,近江牡蛎耗氧率和排氨率变化较大,在14时最大,表明该时点其代谢活动最强.     

甲壳胺寡糖的液相色谱及薄层层析分析

为了解低分子甲壳胺多糖的抗肿瘤辅助疗效,作者利用高效液相色谱法(HPLC)结合薄层色谱法(TLC),探讨了SUGAR-KS-802寡糖分析柱分析甲壳胺寡糖的条件,并尝试了几种薄层层析展开剂对甲壳胺寡糖组分的展层条件。结果表明,在HPLC流动相为0．001mol／L NaOH溶液,流速0．2mL／min时,可将混合的标准甲壳胺寡糖成分很好地分离;采用乙酸乙脂：甲醇：水：氨水=5：9：1：1．5混合液作为硅胶薄板展开剂时,可将不同聚合度的甲壳胺寡糖很好地分开,且层析重现性高,糖残基数目和Rf值具有很好的线性关系。2种分析方法结合可以作为1种可靠的甲壳胺寡糖分析方法。     

氨氮急性攻毒对水产经济动物棘胸蛙(Paa spinosa)蝌蚪死亡率、排氨率、耗氧率及窒息点的影响

以体长(1.932±0.204)cm、体质量(1.386±0.055)g的棘胸蛙蝌蚪为实验动物,在水温(24±0.2)℃、DO(7.30±0.01)mg/L、pH 7.30±0.01条件下,采用静水停食法开展了氨氮对蝌蚪的急性攻毒实验,并以此为基础,测定了不同氨氮质量浓度胁迫下棘胸蛙蝌蚪的排氨率、耗氧率、氧氮比及窒息点。结果表明:(1)蝌蚪对氨氮急性攻毒具明显的运动避毒行为,濒死个体的背部皮肤和肝脏均具明显的色变症状;(2)氨氮对蝌蚪的急性致死率具明显的剂量—时间效应,24h、48h、72h、96h LC50依次为177.5、151.7、148.6和146.8mg/L,毒性时段蓄积程度系数呈持续下降趋势,24—48h、48—72h、72—96h的时段MAC值分别为84.04%、10.1%和5.86%;(3)蝌蚪夜均、昼均、日均及时段排氨率随氨氮质量浓度增加均呈阶梯式下降趋势,其中时段排氨率与对照组均无显著差异的为9.80mg/L实验组,夜均、昼均、日均及时段排氨率与对照组均无显著差异的仅为2.45mg/L实验组(P0.05);(4)氨氮对蝌蚪呼吸耗氧具低毒兴奋效应,其夜均、昼均和日均耗氧率随氨氮质量浓度增加均呈先升后降趋势,峰值氨氮质量浓度范围均为4.90—7.35mg/L(P0.05),与对照组均无显著差异的仅为14.70mg/L实验组(P0.05),窒息点含氧量随氨氮质量浓度增加呈先降后升趋势,谷底出现于4.90mg/L实验组(P0.05),与对照组无显著差异的仅为9.80mg/L实验组(P0.05);(5)实验期间,蝌蚪氧氮比(O:N)波动于18.87—25.34之间,昼均和日均氧氮比(O:N)随氨氮质量浓度增加均依次呈"上升—稳定—下降—再稳定"之趋势,两者的峰值氨氮质量浓度范围分别为4.90—14.70mg/L和4.90—9.80mg/L,夜均氧氮比(O:N)呈先升后降趋势,峰值出现于7.35mg/L实验组(P0.05)。     

Identification of dissolved and particulate carbonyl compounds produced by marine harmful algal bloom species

Carbonyl compounds, especially polyunsaturated aldehydes (PUAs), are increasingly reported as teratogenic to grazers and deleterious to phytoplankton. While PUAs have been considered to be mainly produced by diatoms after cell wounding, little is known about whether microalgae other than diatoms, particularly harmful algal bloom (HAB)-forming species, produce carbonyl compounds. In this study, we analyzed the carbonyl compounds from eight common HAB-forming species ( Akashiwo sanguinea , Karenia mikimotoi , Karlodinium venefi cum , Margalefi dinium polykrikoides , Prorocentrum donghaiense , P . minimum , Scrippsiella trochoidea , and Heterosigma akashiwo ) using gas chromatography-mass spectrometry in full scan and selected ion monitoring (SIM) modes. Our results show a ubiquitous presence of carbonyl compounds in both dissolved and particulate forms in all species we examined. In the full scan mode, 133 chromatographic peaks were detected from 51 samples altogether. Both the varieties of carbonyl compounds and their quantities were algal species dependent, although most of the carbonyl compounds could not be fully identifi ed according to the mass spectral database only due to the unavailability of enough standards for all analytes. Aided with nine standards in SIM mode, we further identifi ed and quantifi ed all nine aldehydes (2-methyl-2-pentenal, trans-2-nonenal, cis-6-nonenal, 2,6-dimethyl-5-heptenal, trans-2-hexenal, trans-2- decenal, 2,4-heptadienal, trans-trans-2,4-octadienal, and trans-trans-2,4-decadienal). Four of these nine aldehydes were detected in particulate form, which confi rmed that trans-2-nonenal could be produced by K . mikimotoi , Karl . venefi cum , P . donghaiense , P . minimum , S . trochoidea , and H . akashiwo , 2,4-heptadienal and trans-trans-2,4-decadienal by A . sanguinea , M . polykrikoides , and S . trochoidea , and trans, trans-2,4- octadienal by S . trochoidea , respectively. We proved that some dinofl agellate and raphidophyte species could contribute to the pool of carbonyl compounds including PUAs in a marine ecosystem. Some carbonyl compounds, particularly those in high cell quota and/or dissolved concentration, may be related to fi shkilling or allelopathy which needs further identifi cation and quantifi cation.     

Seven-frequency VLBI Observations of the GHz-Peaked-Spectrum Source OQ 208

We present quasi-simultaneous VLBI images of the GHz-Peaked-Spectrum radio source OQ 208 obtained with the Very Long Baseline Array at 1.4,1.7,2.3,5.0,8.4,15.4GHz and the European VLBI Network at 6.7 GHz.The low frequency(1.4,1.7 and 2.3GHz)ob- servations reveal a weak and extended steep-spectrum component at about 30mas away at position angle-110°,which may be a remnant emission.The radio structure of OQ 208 con- sists of two mini-lobes at 5.0,6.7,8.4 and 15.4 GHz.Our spectral analysis further confirms that the southwest lobe undergoes free-free absorption and finds that the free-free absorption is stronger in the inner region.By fitting the 8.4 GHz images from 1994 to 2005,we obtain a separation speed of 0.031±0.006 mas yr~(-1)between the two mini-lobes.This indicates a jet proper motion of 0.105±0.020 c and a kinematic age of 219±42 yr for the radio source.     

基于上市公司网络的长三角城市网络空间结构研究

公司内部网络研究是当前城市经济网络研究的重要内容.本文利用2005、2010年长三角A股上市公司企业网络数据库,运用社会网络分析方法、位序—规模分析法等,分析了2005、2010年长三角城市网络空间结构演变及其主要影响因素.研究发现:①长三角上市公司总部沿沪宁—沪杭—杭甬Z字形轴线集聚,从一主(上海)二副(杭州、南京)三中心向一主(上海)三副(杭州、苏州、南京)四中心空间结构演变;苏州超过南京成为第三大企业总部集聚中心.②长三角城市网络核心—边缘结构整体延续,Z字形轴线城市维持核心地位,具备强大的网络权力与威望.③长三角城市网络除等级扩散、邻近扩散等特征之外.还具有明显的行政地域性.主要体现在省会南京、杭州分别成为江苏、浙江省内外上市公司子公司的重要据点;尤其是南京凭借省会优势,中心度仍然高于总部职能较强的苏州.④在长三角经济一体化背景下,跨行政地域性的联系有所加强,特别是上海、苏南向苏中、苏北的扩散明显;浙西南仍是网络相对封闭、孤立的地带,中心度相对较低.⑤行政等级和区划,市场容量等是长三角城市网络空间结构的主要影响因素,而到上海高速公路时间距离、劳动力成本和土地成本的影响在统计上未通过显著性检验.     

冬季和春季长江口及其近海水域浮游病毒丰度的分析

采用荧光显微技术,对2006年长江口及近海水域20个站点的表层及10m层或潜水体冬、春两季的浮游病毒丰度进行了检测,对浮游病毒丰度在季节（冬、春两季）、水平分布和垂直分布上的变化进行了探讨.调查区浮游病毒丰度在冬、春季节上并无明显差异,但在水平分布上存在很大差异,河口区浮游病毒直接检测量（Virus Direct Count, VDC）达到10^7个/ml,近海水域VDC为10^6个/ml,河口区的浮游病毒丰度都明显高于近海水域病毒丰度 （P＜0.01）.在垂直分布上,冬、春两季长江口水域水深小于10m的站位,表层浮游病毒丰度与底层病毒丰度无明显差别,水深大于10m的站位,表层水样的浮游病毒丰度都高于10m水层病毒丰度,说明长江口浮游病毒的垂直分布与站位总水深有关.还通过比较各站点VDC与叶绿素a含量的数据,分析了二者之间的相关性：冬季浮游病毒丰度与叶绿素a含量成正相关性;春季浮游病毒丰度与叶绿素a含量成负相关性,但病毒丰度受叶绿素a含量的影响仅为10%-11%.     

Generation of random waves in time-dependent extended mild-slope equations using a source function method

We develop techniques of numerical wave generation in the time-dependent extended mild-slope equations of Suh et al. [1997. Time-dependent equations for wave propagation on rapidly varying topography. Coastal Engineering 32, 91–117] and Lee et al. [2003. Extended mild-slope equation for random waves. Coastal Engineering 48, 277–287] for random waves using a source function method. Numerical results for both regular and irregular waves in one and two horizontal dimensions show that the wave heights and the frequency spectra are properly reproduced. The waves that pass through the wave generation region do not cause any numerical disturbances, showing usefulness of the source function method in avoiding re-reflection problems at the offshore boundary.     

Farmer-based Rice Cropping Systems in the Poyang Lake Region,China: Analysis of Characteristics and Policy Implications

This paper examines the spatial characteristics of farmer/household behaviors in regional rice cropping systems (RCS), and the results provide necessary information for developing strategies that will maintain regional food security. Through field study and statistical analysis based on 402 households questionnaires finished in 2014-2015 in the Poyang Lake Region (PLR) of China, we arrived at two main conclusions. First, single- and double cropping rice were found across the study area, but showed a general distribution trend, with double cropping rice in the southeast part (especially in Jinxian county) and single cropping rice in the northwest (particularly in De’an county). Second, the household decisions concerning RCS varied in different parts of the PLR, but double cropping was the dominant type, with about 63.57% of the respondent households in the PLR cultivating double cropping rice. However, the multiple-cropping index of paddy rice was only 1.55. About 3% of interviewed households had altered their RCS during this period. Based on these findings, the local governments should guide farmers’ paddy field cultivation behaviors by increasing the comparative efficiency of rice production, promoting appropriate scale operations and land conversion, as well as optimizing rice growing conditions to improve the multiple cropping index and enhance food provision. Finally, land-use efficiency and more sustainable use of land resources should be improved.     

基于GWR模型的中国区域旅行社业效率空间分异及动力机制分析

基于DEA模型对中国31个省域的旅行社业效率空间分异特征进行了分析,首次运用GWR模型探索交通、资本、人才、信息化和经济动力对区域旅行社业效率影响的空间差异。结果表明：旅行社业效率在空间上具有正相关性和集聚特征,空间格局从“川”字型向“山”字型转变。各动力因子的系数均存在空间非平稳性。资本和人才动力的回归系数在空间分布上从南向北依次递减;经济动力的分布趋势为从北向南依次递减;交通动力对中西部地区旅行社效率提升的促进作用显著于东部地区;信息化动力则在东部地区表现出较强的促进作用。