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981.
982.
Haasch ML 《Marine environmental research》2002,54(3-5):297-301
The mummichog (Fundulus heteroclitus) has been shown to be responsive to peroxisome proliferating agents (PPAs). Peroxisomes function as important sites for fatty acid beta-oxidation. Peroxisome proliferation by PPAs or starvation can lead to changes in the size and number of peroxisomes and the expression of omega-hydroxylases (CYP2K1/2M1 in rainbow trout). Mummichogs were subjected to 96 h fasting or 96 h recovery from fasting. Expression of PMP70- and CYP2K1/2M1-like proteins in vehicle-treated or non-treated controls was compared in both males and females. Fasting and vehicle produced decreases in PMP70- and CYP2K1/2M1-like proteins in both males and females. In reproductive females, decreases due to fasting and vehicle treatment were greater than in female fish that were not gravid. Recovery from fasting resulted in levels of CYP2K1/2M1 near control levels in males while in recovered females, about 2-fold higher levels compared to controls were noted. These results indicate that gender, reproductive status and diet can produce changes in the expressed levels of peroxisomal PMP70 and microsomal CYP2K1/2M1-like proteins in the mummichog. 相似文献
983.
It is demonstrated that the Jason-1 measurements of sea surface height (SSH), wet path delay, and ionosphere path delay are within required accuracies, via a global cross-calibration with similar measurements made by TOPEX/Poseidon (T/P) over a 6-month period. Since the two satellites were on the same groundtrack separated in time by only 70 s, measurements were recorded at approximately the same location and time. The variations in the wet path delay measured by Jason-1 compared to T/P are only 5 mm RMS, well within the required performance of 1.2 cm RMS. The RMS of the ionosphere differences is also well within the expected values, with a mean RMS of 1.2 cm. The largest difference is that the Jason-1 SSH is biased high relative to T/P SSH by 144 mm after the T/P and Jason-1 data are both corrected with improved sea state bias (SSB) models. However, the bias will change if a different SSB model is used, so the user should be cautious that the bias used matches the SSB models. The bias is generally constant within ± 10 mm in the open ocean, but appears to be higher or lower in some regions. Additionally, the SSH has been verified by comparison with 36 island tide gauges over the same period. After removing the global relative bias, the Jason-1 SSH data agree with tide gauges within 3.7 cm RMS and with T/P data within about 3.5 cm RMS on average for 1-s measurements, meeting the required accuracy of 4.2 cm RMS. 相似文献
984.
985.
Our goal was to study the involvement of cytochrome P450 genes in estrogen metabolism and the extent to which the potentially carcinogenic 4-hydroxyestradiol metabolite is formed by channel catfish (Ictalurus punctatus; CC). Estradiol metabolism and ethoxyresorufin-O-deethylase (EROD) activity were assessed in several tissues from fish collected from three variably contaminated sites in the Mississippi River Delta, from laboratory control fish, and from fish exposed to 20 mg/kg benzo(a)pyrene (BaP) i.p. for 4 days. Liver EROD activity was induced by BaP, but Delta fish EROD activities were not statistically higher than activities in control fish. Gill microsomal EROD activity was also induced by BaP, but activities were 8- to 77-fold lower than those from liver. The predominant estrogen metabolites formed by CC liver, gill and gonad microsomes were 2-hydroxyestradiol and estrone as detected by GC/MS. Liver and gill 2-hydroxyestradiol formation was induced in BaP-dosed fish. The trends in hydroxyestradiol formation in field collected fish were more variable. In all fish liver microsomes there was more 2- compared to 4-hydroxyestradiol formed. However, BaP-treatment increased the 4:2 hydroxyestradiol ratio from 0.04 in control fish to 0.2 in BaP-exposed fish, suggesting that BaP induces the formation of the potentially genotoxic estrogen metabolite. No detectable 4-hydroxyestradiol was produced by gill and gonad microsomes. These results will ultimately help in determining which fish P450 genes are susceptible to environmental contamination and may be involved in estrogen genotoxicity. 相似文献
986.
大菱鲆产卵季节对卵子的生物学及生化特征的影响 总被引:13,自引:10,他引:13
于2000年6-8月,采用现场测定和生化分析方法,研究了大菱Ping雌鱼产卵盛期和产卵晚期卵子的生物学特征及生化组成。结果表明,大菱Ping卵子的生物学特征及生化组成随着产卵季节的进程而改变。产卵晚期的卵子卵径小于产卵盛期,每粒卵子含水量及重量均高于晚期,盛期卵子的粗蛋白、脂肪、灰分占干重的百分比低于晚期卵子。在产卵晚期,中性脂所产卵盛期有明显增加,而20:5(n-3)、20:4(n-6)水平比产卵盛期有明显增加,而20:5(n-3)、20:4(n-6)、22:5(n-3)和22:6(n-3)的水平则明显下降。产卵盛期卵子的孵化率和存活力要高于晚期。 相似文献
987.
988.
本文报道了网片剪裁计算理论,网片单、双完成计算公式,正确的网片剪裁单、双完成计算理论和计算公式,对国外的几何作图和网片剪裁循环比例的基本计算理论,作了进一步的网片剪裁实践和理论对比。结果得出,本文的理论和计算公式是正确的。 相似文献
989.
We present calibration results from Jason-1 (2001-) and TOPEX/POSEIDON (1992-) overflights of a California offshore oil platform (Harvest). Data from Harvest indicate that current Jason-1 sea-surface height (SSH) measurements are high by 138 ± 18 mm. Excepting the bias, the high accuracy of the Jason-1 measurements is in evidence from the overflights. In orbit for over 10 years, the T/P measurement system is well calibrated, and the SSH bias is statistically indistinguishable from zero. Also reviewed are over 10 years of geodetic results from the Harvest experiment. 相似文献
990.