Organochlorines and dioxin-like compounds in green-lipped mussels Perna viridis from Hong Kong mariculture zones

Concentrations of persistent organic pollutants including polychlorinated biphenyls (PCBs), organochlorine (OC) pesticides and dioxin-like compounds were measured in green-lipped mussels, Perna viridis, collected from seven mariculture zones in Hong Kong between September and October in 2002 in order to evaluate the status, spatial distribution and potential sources of pollution in these areas. Concentrations ranged from 300 to 4400 ng/g lipid weight for total OCs and 170–1000 ng/g lipid weight for total PCBs (based on 28 congeners). Relatively smaller DDT concentrations in mussels compared with previous studies suggest reduced discharges of DDTs from nearby regions into Hong Kong waters. Detection of a mixture of HCH isomers in the mussels indicated that Hong Kong waters were predominantly contaminated by technical HCHs rather than lindane. Mussel samples from all sampling locations elicited significant dioxin-like activity in the H4IIE-luc bioassay. The greatest magnitude of dioxin-like response (39 pg TEQ/g wet wt.) was detected in mussels from Ma Wan in the western waters of Hong Kong, which is strongly influenced by the Pearl River discharge. Human health risk assessment was undertaken to evaluate potential risks associated with the consumption of the green-lipped mussels. Risk quotient (RQ) for dioxin-like compounds was greater than unity suggesting that adverse health effects may be associated with high mussel consumption.     

cDNA cloning and expression of a cytochrome P450 1A (CYP1A) gene from the hermaphroditic fish Rivulus marmoratus

We previously described the genomic structure of the cytochrome P450 1A (CYP1A) gene from the hermaphroditic fish Rivulus marmoratus [Kim, I.-C., Kim, Y.J., Yoon, Y.-D, Kawamura, S., Lee, Y.-S., Lee, J.-S., 2004a. Cloning of cytochrome P450 1A (CYP1A) genes from the hermaphroditic fish Rivulus marmoratus and the Japanese medaka Oryzias latipes. Mar. Environ. Res. 58, 125–129]. To further characterize R. marmoratus CYP1A, we cloned the cDNA sequence of a CYP1A gene from this species and also expressed its recombinant protein in an E. coli system. We exposed R. marmoratus to 4-nonylphenol, and found a small induction of CYP1A mRNA in the treated animals. In this paper, we discuss the characteristics of R. marmoratus CYP1A gene as well as its potential use in a biomonitoring assay.     

EFFECT OF PERPENDICULAR A.C. ELECTRIC FIELD ON THE OBLIQUE WHISTLER MODE INSTABILITY IN THE EARTH’S MAGNETOSPHERE

The elements of dielectric tensor and dispersion relation for obliquely propagating whistler waves with finite in an infinite magnetoplasma are obtained for a kappa distribution in the presence of perpendicular a.c. electric field. Integrals and modified plasma dispersion functions are reduced in power series form. Numerical calculations have been performed to obtain temporal growth rate and real frequencies of the plasma waves for magnetospheric plasma, using linear theory of dispersion relation. The effect and modification introduced by the perpendicular a.c. electric field on the temporal growth rates, real frequencies and resonance condition are discussed for kappa and Maxwellian distributions. Our results and their interpretation are compared with known whistler observations obtained by ground-based techniques and satellite observations.     

A technique for modeling the Earth’s gravity field on the basis of satellite accelerations

A technique is proposed for Earths gravity field modeling on the basis of satellite accelerations that are derived from precise orbit data. The functional model rests on Newtons second law. The computational procedure is based on the pre-conditioned conjugate-gradient (PCCG) method. The data are treated as weighted average accelerations rather than as point-wise ones. As a result, a simple three-point numerical differentiation scheme can be used to derive them. Noise in the orbit-derived accelerations is strongly dependent on frequency. Therefore, the key element of the proposed technique is frequency-dependent data weighting. Fast convergence of the PCCG procedure is ensured by a block-diagonal pre-conditioner (approximation of the normal matrix), which is derived under the so-called Colombo assumptions. Both uninterrupted data sets and data with gaps can be handled. The developed technique is compared with other approaches: (1) the energy balance approach (based on the energy conservation law) and (2) the traditional approach (based on the integration of variational equations). Theoretical considerations, supported by a numerical study, show that the proposed technique is more accurate than the energy balance approach and leads to approximately the same results as the traditional one. The former finding is explained by the fact that the energy balance approach is only sensitive to the along-track force component. Information about the cross-track and the radial component of the gravitational potential gradient is lost because the corresponding force components do no work and do not contribute to the energy balance. Furthermore, it is shown that the proposed technique is much (possibly, orders of magnitude) faster than the traditional one because it does not require the computation of the normal matrix. Hints are given on how the proposed technique can be adapted to the explicit assembling of the normal matrix if the latter is needed for the computation of the model covariance matrix.Acknowledgments. Professor R. Klees is thanked for support of the project and for numerous fruitful discussions. The authors are also thankful to Dr. J. Kusche for useful remarks and to Dr. E. Schrama, his solid background in satellite geodesy proved to be very helpful. A large number of valuable comments were made by Dr. S.-C. Han, Dr. P. Schwintzer, and an anonymous reviewer; their contribution is greatly acknowledged. The satellite orbits used in the numerical study were kindly provided by Dr. P. Visser (Aerospace Department, Delft University of Technology). Access to the SGI Origin 3800 computer was provided by Stichting Nationale Computerfaciliteiten (NCF), grant SG-027.     

IMPACT OF DIABATIC PROCESSES IN AGCM ON 4-DIMENSIONAL VARIATIONAL DATA ASSIMILATION*

The impact of diabatic processes on 4-dimensional variational data assimilation (4D-Var) was studied using the 1995 version of NCEP's global spectral model with and without full physics.The adjoint was coded manually.A cost function measuring spectral errors of 6-hour forecasts to "observation" (the NCEP reanalysis data) was minimized using the L-BFGS (the limited memory quasi-Newton algorithm developed by Broyden,Fletcher,Goldfard and Shanno) for optimizing parameters and initial conditions.Minimization of the cost function constrained by an adiabatic version of the NCEP global model converged to a minimum with a significant amount of decrease in the value of the cost function.Minimization of the cost function using the diabatic model, however,failed after a few iterations due to discontinuities introduced by physical parameterizations.Examination of the convergence of the cost function in different spectral domains reveals that the large-scale flow is adjusted during the first 10 iterations,in which discontinuous diabatic parameterizations play very little role.The adjustment produced by the minimization gradually moves to relatively smaller scales between 10-20th iterations.During this transition period,discontinuities in the cost function produced by "on-off" switches in the physical parameterizations caused the cost function to stay in a shallow local minimum instead of continuously decreasing toward a deeper minimum. Next,a mixed 4D-Var scheme is tested in which large-scale flows are first adiabatically adjusted to a sufficient level,followed by a diabatic adjustment introduced after 10 to 20 iterations. The mixed 4D-Var produced a closer fit of analysis to observations,with 38% and 41% more decrease in the values of the cost function and the norm of gradient,respectively,than the standard diabatic 4D-Var,while the CPU time is reduced by 21%.The resulting optimal initial conditions improve the short-range forecast skills of 48-hour statistics.The detrimental effect of parameterization discontinuities on minimization was also reduced.     

Noril’sk–Talnakh Cu–Ni–PGE deposits: a revised tectonic model

The Norilsk mining district is located at the northwest margin of the Tunguska basin, in the centre of the 3,000×4,000 km Siberian continental flood basalt (CFB) province. This CFB province was formed at the Permo-Triassic boundary from a superplume that ascended into the geometric centre of the Laurasian continent, which was surrounded by subducting slabs of oceanic crust. We suggest that these slabs could have reached the core–mantle boundary, and they may have controlled the geometric focus of the superplume. The resulting voluminous magma intruded and erupted in continental rifts and related extensive flood basalt events over a 2–4 Ma period. Cu–Ni–PGE sulfide mineralization is found in olivine-bearing differentiated mafic intrusions beneath the flood basalts at the northwestern margin of the Siberian craton and also in the Taimyr Peninsula, some 300 km east of a triple junction of continental rifts, now buried beneath the Mesozoic–Cenozoic sedimentary basin of western Siberia. The Norilsk-I and Talnakh-Oktyabrsky deposits occur in the Norilsk–Kharaelakh trough of the Tunguska CFB basin. The Cu–Ni–PGE-bearing mineralized intrusions are 2–3 km-wide and 20 km-long differentiated chonoliths. Previous studies suggested that parts of the magma remained in intermediate-level crustal chambers where sulfide saturation and accumulation took place before emplacement. The 5–7-km-thick Neoproterozoic to Palaeozoic country rocks, containing sedimentary Cu mineralization and evaporites, may have contributed additional metal and sulfur to this magma. Classic tectonomagmatic models for these deposits proposed that subvertical crustal faults, such as the northeast-trending Norilsk–Kharaelakh fault, were major trough-parallel conduits providing access for magmas to the final chambers. However, geological maps of the Norilsk region show that the Norilsk–Kharaelakh fault offsets the mineralization, which was deformed into folds and offset by related reverse faults, indicating compressional deformation after mineralization in the Late Triassic to Early Jurassic. In addition, most of the intrusions are sills, not dykes as should be expected if the vertical faults were major conduits. A revised tectonic model for the Norilsk region takes into account the fold structure and sill morphology of the dominant intrusions, indicating a lateral rather than vertical emplacement direction for the magma into final chambers. Taking into account the fold structure of the country rocks, the present distribution of the differentiated intrusions hosting the Norilsk-I and Talnakh–Oktyabrsky deposits may represent the remnants of a single, >60 km long, deformed and eroded palm-shaped cluster of mineralized intrusions, which are perceived as separate intrusions at the present erosional level. The original direction of sill emplacement may have been controlled by a northeast-trending paleo-rise, which we suggest is present at the southeastern border of the Norilsk–Kharaelakh trough based on analysis of the unconformity at the base of the CFB. The mineralized intrusions extend along this rise, which we interpret as a structure that formed above the extensionally tilted block in the metamorphic basement. Geophysical data indicate the presence of an intermediate magma chamber that could be linked with the Talnakh intrusion. In turn, this T-shaped flat chamber may link with the Yenisei–Khatanga rift along the northwest-trending Pyasina transform fault, which may have served as the principal magma conduit to the intermediate chamber. It then produced the differentiated mineralized intrusions that melted through the evaporites with in situ precipitation of massive, disseminated, and copper sulfide ore. The Norilsk–Kharaelakh crustal fault may not relate to mineralization and possibly formed in response to late Mesozoic spreading in the Arctic Ocean.Editorial handling: P. Lightfoot     

Structural changes and valence states in the MgCr<Subscript>2</Subscript>O<Subscript>4</Subscript>–FeCr<Subscript>2</Subscript>O<Subscript>4</Subscript> solid solution series

The influence on the structure of Fe²⁺ Mg substitution was studied in synthetic single crystals belonging to the MgCr₂O₄–FeCr₂O₄ series produced by flux growth at 900–1200 °C in controlled atmosphere. Samples were analyzed by single-crystal X-ray diffraction, electron microprobe analyses, optical absorption-, infrared- and Mössbauer spectroscopy. The Mössbauer data show that iron occurs almost exclusively as ^IVFe²⁺. Only minor Fe³⁺ (<0.005 apfu) was observed in samples with very low total Fe. Optical absorption spectra show that chromium with few exceptions is present as a trivalent cation at the octahedral site. Additional absorption bands attributable to Cr²⁺ and Cr³⁺ at the tetrahedral site are evident in spectra of end-member magnesiochromite and solid-solution crystals with low ferrous contents. Structural parameters a₀, u and T–O increase with chromite content, while the M–O bond distance remains nearly constant, with an average value equal to 1.995(1) Å corresponding to the Cr³⁺ octahedral bond distance. The ideal trend between cell parameter, T–O bond length and Fe²⁺ content (apfu) is described by the following linear relations: a₀=8.3325(5) + 0.0443(8)Fe²⁺ (Å) and T–O=1.9645(6) + 0.033(1)Fe²⁺ (Å) Consequently, Fe²⁺ and Mg tetrahedral bond lengths are equal to 1.998(1) Å and 1.965(1) Å, respectively.     

Stable carbon isotope ratios in lake and swamp sediments as a proxy for prehistoric forest clearance and crop cultivation in the Neotropics

Close correspondence between stable carbon isotope ratios ( ¹³ C), pollen, and charcoal profiles in sediment cores from Laguna Zoncho and Machita swamp, Costa Rica, shows that prehistoric forest clearance and crop cultivation can be detected in the stable carbon isotope ratios of total organic carbon ( ¹3C _TOC ). Analyses of δ 13C _TOC complement evidence from pollen, charcoal, and phytoliths and provide a proxy that is sensitive to the intensity and/or proximity to core sites of prehistoric forest clearance and agriculture in watersheds. Stable carbon isotope analyses are particularly useful in situations in which other evidence of forest clearance and agriculture is limited.