口蹄疫病毒VP1植物表达载体的构建

P1 T重组质粒上含有口蹄疫病毒 (FMDV)GD10分离株的p1cDNA片段 ,以此为模板 ,用PCR方法扩增其中的VP1基因 ,获得大小约 6 40bp的片段。该片段用BglⅡ和BstEⅡ酶切消化后克隆至表达载体 pCAMBIA130 5 .2 ,转化EcoliTOP10感受态细胞。重组质粒经PCR、酶切及序列分析 ,证实VP1基因处于CaMV35S启动子控制 ,且读码框正确     

正交试验法在大骨节病区饮水水质研究中的应用

本文运用正交试验法对陕西永寿等大骨节病区饮水水质及其与病情间的关系进行了研究和试验,取得了如下初步认识:1)饮用缺乏Mg~(2+)、C1~-、SO_4~(2-)、HCO_3~-、Se、Sr等组分的低矿化度水,是导致大骨节病发生的重要原因。2)水中的Na~++K~+、Mg~(2+)、Ca~(2+);C1~-、SO_4~(2-)、HCO_3~-;可溶性SiO_2、有机耗氧量、矿化度;Sr、Se、Zn等含量(或阴、阳离子毫克当量百分数)的任何一组最优组合,均可用来作为优质水的划分标准。在此基础上,确定出了非致病水的水质标准。文章还对所确定出的非致病水水质标准进行了实际验证。     

饲料蛋白水平对奥尼罗非鱼(Oreochromis niloticus × O. aureus)生长、免疫功能以及抗病力的影响

采用单因素实验设计方法,进行了饲料蛋白水平对奥尼罗非鱼(Oreochromis niloticus×O.aureus)生长、免疫功能和抗无乳链球菌能力影响的研究。结果表明,饲料蛋白水平对奥尼罗非鱼生长和饲料利用率以及成活率不产生显著影响,蛋白质效率和蛋白质沉积率伴随着蛋白水平的升高显著下降。35%(D35)饲料组奥尼罗非鱼肝脏过氧化氢酶含量显著高于其它饲料组,然而奥尼罗非鱼肝脏溶菌酶、超氧化物歧化酶活性以及丙二醛含量并不受饲料蛋白水平的影响。无乳链球菌攻毒实验结果表明,蛋白水平对奥尼罗非鱼在攻毒后24h和48h后的累积死亡率是不存在显著影响。本实验的结果表明,24.8%(D25)蛋白水平饲料能够满足奥尼罗非鱼正常生长、以及维持机体正常的非特异性免疫功能和抗病力的营养需求。     

青海省典型土遗址病害及动力测试分析

通过调查青海省四处典型土遗址发现表面剥蚀、冲沟、裂缝、坍塌、人为破坏等病害普遍存在,门源古城和湟中县部分长城因缺乏保护,人为破坏情况严重,土遗址保存状况恶劣;大通县长城和湟中县加牙烽燧在开展保护工程后,土遗址整体保存状况良好。大通县长城2处墙体的现场脉动观测结果显示:（1）2处墙体的速度峰值随高度增加有明显放大现象,一号墙体由底部到顶部速度峰值放大1.7倍,二号墙体放大2.9倍,放大效应让2处墙体在地震时更不稳定;（2）两处墙体的测试频率在3.04 Hz至5.71 Hz之间,通过计算获得一、二号墙体差值百分比分别为30.5%和11.1%,表明二号墙体与场地相互作用明显,处于高风险影响,当遭遇地震的卓越频率与之相近时会发生明显的共振现象。因此应对观测处墙体进行加固,以减小与场地的相互作用,提高墙体的抗震稳定性。     

指示冬小麦条锈病严重度的两个新的红边参数

通过人工田间诱发不同等级条锈病,在不同生育期测定了36条感染不同严重程度条锈病的冬小麦冠层光谱及相应叶片的生理生化参量。对测定的冬小麦红边一阶微分光谱进行分析,发现随着病情严重度的增加,红边一阶微分的前峰(700nm附近)越来越明显,后峰(约在725—740nm)越来越不突出,以红边一阶微分的双峰特征随病情指数的变化为基础,设计了两个新型的红边参数:DSr和Ar,它们可以分别用来描述红边一阶微分光谱曲线的陡峭度和不对称性,与其他常用的红边参数(红边位置、红边一阶微分最大值,红边一阶微分所包围面积)相比,新参数反演病情严重度的精度更高。     

气候异常对2007年度许昌小麦生产的影响

分析了2007年度气候异常引起的农业气象灾害和病虫害及其对小麦生育的影响,并提出了应对气候异常对许昌小麦生产影响的对策。     

Identification of Anomalous Aluminium Contents in Surface and Ground Water in the Alba Territory (NW Italy)

Aluminium was determined in surface and ground waters in the Alba region of NW Italy to provide information on natural and anthropogenic sources of contamination. Determinations were made by a spectrophotometric method based on the aluminium-pyrocatechol complex. The total aluminium content of waters was determined as well as the toxic form of monomeric aluminium, obtained from samples that had been passed through 0.20 μm cellulose filters to remove colloidal and particulate material. Results were correlated with mortality data associated with Alzheimer's disease. There appeared to be a correlation between mortality data and areas having the highest aluminium content of water.     

Health impacts of coal and coal use: possible solutions

Coal will be a dominant energy source in both developed and developing countries for at least the first half of the 21st century. Environmental problems associated with coal, before mining, during mining, in storage, during combustion, and postcombustion waste products are well known and are being addressed by ongoing research. The connection between potential environmental problems with human health is a fairly new field and requires the cooperation of both the geoscience and medical disciplines. Three research programs that illustrate this collaboration are described and used to present a range of human health problems that are potentially caused by coal. Domestic combustion of coal in China has, in some cases, severely affected human health. Both on a local and regional scale, human health has been adversely affected by coals containing arsenic, fluorine, selenium, and possibly, mercury. Balkan endemic nephropathy (BEN), an irreversible kidney disease of unknown origin, has been related to the proximity of Pliocene lignite deposits. The working hypothesis is that groundwater is leaching toxic organic compounds as it passes through the lignites and that these organics are then ingested by the local population contributing to this health problem. Human disease associated with coal mining mainly results from inhalation of particulate matter during the mining process. The disease is Coal Worker's Pneumoconiosis characterized by coal dust-induced lesions in the gas exchange regions of the lung; the coal worker's “black lung disease”.     

基于国际比较视角的北京“四个中心”建设体检评估探索

在梳理国内外城市规划评估体检实践与研究进展、总结北京城市体检评估机制的基础上,构建北京“四个中心”建设体检评估的框架,基于国际比较视角遴选指标定性定量分析相结合进行体检评估。体检评估结果表明：① 北京的全国政治中心服务保障能力显著提升,展现出大国首都形象;② 北京的全国文化中心地位进一步增强,但公共文化服务供给能力与世界知名城市存在差距,缺少具有国际影响力的文化品牌;③ 虽然国际交往环境及配套服务水平不断提升,但北京的国际组织规模影响力与大国首都地位不匹配,对国际游客的吸引力不强;④ 尽管北京已初步建成具有全球影响力的科技创新中心,创新对高质量发展支撑能力待增强。未来,北京应当坚持以首都发展为统领,大力加强“四个中心”功能建设、提高“四个服务”水平,提升在全球城市体系中的地位,扩大北京作为大国首都在世界的影响力。北京城市体检应重视城市定位体检、加强各参与方的交流合作、拓宽体检评价的国际视野、动态调整体检评价指标、探索高频时空数据分析技术的应用。