Rapid detection of virulent protease secreted by Vibrio anguillarum by dot enzyme-linked immunosorbent assay

Dot enzyme-linked immunosorbent assay （dot-ELISA）, indirect ELISA and Westem blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Westem blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5-6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards （＂marker＂） so that the alterations ofprotease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10^4 cell/mL in the indirect ELISA, while 10^5 cell/mL in the dot-ELISA.     

GPS数据采集技术及其在海洋调查中的应用

本文应用VB6．0开发了智能化的GPS数据采集软件．对该软件的功能进行了简单的介绍，同时对一些关键技术作了较详细的描述。利用该软件、GPS接收机及PC机的组合，可对走航式测量仪器(或移动设备)进行实时定位并保存定位记录，且大大减少一些不必要的数据，使得软件更为实用。     

关于“包装产品的计量标准型一次抽检方案”的确定

本文给出了“包装产品的计量标准型一次抽检方案”,并通过一个实例说明本方案比一般《计量标准型一次抽检方案》更为优越。     

海洋遥感四维测量应用

机载成象光谱仪遥感海洋是在轨星载海洋传感器定标校验的一种方法。本文研究了海洋航空遥感的四维测量方法，以及实现卫星、飞行遥感和船舶同步测量的重要意义。     

底部浮泥表层推移速度分布的ADCP—GPS估测方法

ADCP对底跟踪走航观测的流速数据中包含水体底部浮泥、底沙运动信息,对比GPS定位方法算出的水体流速数据可以分离出浮泥相对于GPS定位的运动信号,从而达到对底质推移观测的目的。     

使用GAMIT进行高精度基线向量解算的方法与实践

GAM IT是国际上常用的GPS定位定轨软件,但由于其安装的平台(UN IX/LINUX)与我们通用的W indow s平台有所差异,使得GAM IT的入门较难。因此本文结合具体实例,介绍GAM IT的安装、基本操作以及应用GAM IT进行高精度基线向量解算,供初学者参考。     

建立我国沿海永久GPS陆海垂直运动监测网的建议

论述了在我国沿海建立永久GPS陆海垂直运动监测风的目的和意义，介绍了有关的国内外发展现状，监测的方法和原理，最后提出了在我国建立永久GPS陆海垂直运动监测网的具体建议。     

基于强跟踪性能的QEKF组合导航自适应滤波研究

以低成本陆用航姿参考系统（AHRS）/GPS紧耦合系统为研究对象,建立了基于伪距、伪距率、航向角的组合观测数学模型。引入强跟踪滤波（STF）算法,利用渐消因子的作用,增强滤波器对状态快速变化的跟踪能力。同时,针对微惯性器件漂移过大的缺点,采用二阶EKF（QEKF）方法,通过对Hessian矩阵的求解,补偿系统观测方程线性化的二阶截断误差。仿真表明：STQEKF方法可高速准确地逼近系统非线性模型,实现陆地载体导航控制,在传感器精度有限的情况下,使姿态和位置的控制效果较标准EKF分别提高了约8.9%-38.2%和48.7%-54.4%     

两种地方坐标系统建立方法的对比

针对不同的情况需要建立不同的坐标系,介绍了两种不同的地方坐标系统建立方法,并对这两种方法进行了比较分析。分析结果表明,可以根据具体的情况下选用不同的方法。本文介绍的方法对工程应用有一定的帮助。     

露天矿GPS卡车调度系统数据流处理方法

露天矿GPS卡车调度系统是数字矿山建设中的重要组成部分,是露天矿生产管理利用GPS／GIS技术提高生产效益的有效途径之一。本文根据露天矿GPS卡车调度系统的组成、工作原理和数据流交换模式,提出了基于数据回放条件下的装卸车时序匹配计算方法以及数据流处理的主要内容。抚顺西露天矿的实践证明,该计算方法是一种处理卡车调度系统数据流的有效且可行的办法。