基于特定序列的长牡蛎视黄酸反应元件预测

视黄酸受体和核受体超家族中的大部分成员对细胞整个分化、增殖过程都具有调控功能。视黄酸受体结合配体后激活,通过结合靶基因启动子区特定的核苷酸序列调控靶基因表达。视黄酸受体结合序列是由核心序列[A/G]G[T/G]TCA间隔不同碱基构成的重复序列,称为视黄酸反应元件。为了实现对长牡蛎基因组中含有的视黄酸反应元件的快速筛选预测,本研究利用Perl工具编写了一个可以批量筛选视黄酸反应元件的脚本,并对长牡蛎基因组中启动子区域序列进行筛选预测,共筛选到412个启动子区含有视黄酸反应元件的基因。随后,将这些基因在各种数据库中比对分析,预测其参与的生物学过程及可能的生物学功能。结果显示,大部分基因与蛋白质结合、核苷酸结合、水解酶活性、蛋白激酶活性等功能有关。     

南四湖湖内浅槽工程施工对水环境的影响及控制措施

南四湖湖内工程是沂沭泗河洪水东调南下续建工程的单项工程,工程区位于山东省省级自然保护区内;挖泥船施工将对湖内水环境产生一定影响。本文从排泥场回水污染物、生产废水和生活污水三个方面阐述了工程施工对水环境的影响程度,并且提出了相应的控制措施。     

夏季长江口东北部上升流海域的生态环境特征

基于2006年7月18—23日对长江口东北部海域的大面调查,重点分析和探讨了长江口东北部海域的上升流现象及生态环境特征。结果显示：（1）大约在122°—123°E,32.3—33.7°N的海域范围内存在着较为明显的下层高盐冷水抬升的现象。（2）伴随着冷水的上升运动,对DO、营养盐以及Chl.a的平面和断面分布均存在一定...     

海岸工程中海浪数值模拟和环境参数选取的进展

主要介绍了我国近的来在海岸工程设计，建设中有关海浪数值模拟及环境参数选取方面的研究进展。     

江苏金坛新石器时代环境变迁研究

对江苏金坛三星村新石器遗址ＪＳ９８Ｔ０８２０号探坑南壁剖面进行采磁,对样品帮了粒度、地球化学、孢粉、微体古生物（有孔虫和介形类）等环境替代指标的测定与分析,并据此初步研究和恢复了该区新石器时代古定变状况,得出以下几点认识：（１０地球化学分析结果本区知全新世以来,气候有从暖湿向干冷变化的总趋势。（２）Ｐ２Ｏ５的含量在马家浜墓葬层和崧泽文化层可达生土层的５～６倍,充分反映了当时人类活动日益频繁,同时也     

赤点石斑鱼(Epinephelus akaara)精子超微结构及环境因子对精子活力的影响

为揭示赤点石斑鱼精子的超微结构及环境因子对其精子活力的影响,利用扫描电镜和透射电镜观察赤点石斑鱼精子的超微结构,设置不同梯度的温度、盐度、pH值及不同浓度的NaCl、KCl、CaCl₂、MgCl₂、EDTANa₂溶液,探究这些因子对赤点石斑鱼精子活力的影响。结果表明:赤点石斑鱼成熟精子的结构特点是细胞核圆形或卵圆形,核内染色质致密,没有核泡(核空隙)。精子尾部细长,横切面为典型的"9+2"微管结构。温度、盐度、pH值等环境因子对精子活力的影响表明,精子活力的适宜温度范围为23~31℃,27.5℃时精子寿命最长为37min;适宜的盐度范围为15~35,盐度15时精子寿命最长为50min;适宜的pH范围为7~9,pH为9时精子的运动时间最长为33min。赤点石斑鱼精子在EDTANa₂溶液中呈抑制状态,在400~700 mmol/L的NaCl溶液、400~600mmol/L的KCl溶液和500 mmol/L的CaCl₂溶液中精子均具有较好的活动能力。在MgCl₂溶液中赤点石斑鱼精子的活动能力不佳。赤点石斑鱼精子的最适温度范围与繁殖季节的最适水温范围符合,适宜盐度范围较广,对pH值的变化适应性较强。赤点石斑鱼精子在NaCl、KCl、CaCl₂溶液中活力较佳,在MgCl₂溶液和EDTANa₂溶液中呈抑制状态。     

Variable orientation within a natural population of the sandhopper Talitrus saltator (Crustacea: Amphipoda) as a response to a variable environment: The case-study of Berkoukesh beach,Tunisia

Sandy beaches are harsh environments, driving resident arthropod populations to various typical adaptations, particularly behavioural ones. Here we evaluated the effects of seasonal meteorological variability on the behaviour of Talitrus saltator on Berkoukesh beach (N-W Tunisia). The site is characterised by a Mediterranean climate, but is particularly exposed to seasonal winds and storms. The shoreline is in morphodynamic equilibrium. We tested sandhopper Talitrus saltator orientation in April, when sudden rainfall and storms are common, and in June, when as a rule the weather is warm and dry. The results were analysed with circular statistics and multiple regression models adapted to angular distributions, in order to highlight differences in orientation under the various conditions. Depending on the environmental conditions, amphipods from the same population appeared to utilise various orientation strategies as a response to different environmental constraints. The use of a range of behavioural mechanisms (sun-orientation seaward, sun-orientation landward, and phototaxis) resulted in links to the local landscape and to the animals’ life cycle. As a general conclusion, we can infer that the behavioural variability found within the same population represents a response to seasonal environmental fluctuation. Such an increase in variability is likely to develop on a beach in dynamic equilibrium, where landscape references are stable, and a variable behaviour represents a strategy for dealing with environmental fluctuations.     

Abundance,distribution, and feeding patterns of juvenile coho salmon (Oncorhynchus kisutch) in the Juan de Fuca Eddy

The Juan de Fuca Eddy is a seasonal, counter-clockwise gyre off the mouth of the Strait of Juan de Fuca between Washington, USA and British Columbia, Canada that may provide favorable feeding habitat for juvenile coho salmon (Oncorhynchus kisutch) during their early marine existence. In late September 2002, physical and biological sampling was conducted along two transects of the eddy region. Surface rope trawling was conducted to capture juvenile salmon and other nekton, along with bongo and neuston net tows to examine potential mesozooplanktonic salmon prey. Presence of the Juan de Fuca Eddy was confirmed with vertical water profiles. In addition, nutrient and chlorophyll a concentrations collected from 3-m depth were within the range observed in previous studies within the eddy region. In the mesozooplankton community, euphausiids, chaetognaths, and decapod megalopae were common. In the diet of juvenile coho salmon, euphausiids and decapod megalopae were dominant by percent number, and larval and juvenile fish were dominant by percent weight. Feeding intensity (percent body weight) based on stomach contents was variable, but not significantly different among stations. To compare the Juan de Fuca Eddy region with an upwelling area, we sampled along a transect off La Push (LP), Washington, USA which is south of the eddy. The eddy region was found to be less productive than the LP transect. Nutrients were lower, chlorophyll a concentrations were higher, and zooplankton abundance was generally higher along the LP transect than in the eddy region. In addition, more juvenile coho salmon were captured from the LP transect than the eddy region. Prey items in stomachs of salmon from the LP transect were heterogeneous compared to those from the eddy region. Feeding intensity along the LP transect was slightly lower and more variable than in the eddy region, and differences in feeding intensity among LP stations were significant. In addition, feeding intensities among stations nested within regions were significantly different.     

Momentum and gravity effects during the constant velocity water entry of wedge-shaped sections

Computational fluid dynamics analysis was used to investigate the added mass momentum, flow momentum and gravity effects during the constant velocity water entry of wedge-shaped sections with deadrise angles from 5° to 45°. It is shown that the added mass continues to increase for a time after chine immersion and that added mass can be estimated in terms of a constant added mass coefficient and an effective wetted width. A momentum theory is presented in which the water entry force is explained as the sum of the rate of change of added mass momentum, which becomes zero at immersion to chine depth ratios greater than about three, and the rate of change of flow momentum, which continues at deep immersions. The effect of gravity on the water entry force is given as the hydrostatic force together with the force necessary to create the potential energy in the water pile up. Hydrodynamic forces are not significantly changed by the effect of gravity on the flow fields.