白缘(鱼央)5S rDNA的扩增分析及其在染色体上的FISH定位

本文分别对雌雄白缘(鱼央)的5S rDNA进行了PCR扩增和序列分析,并采用双色荧光原位杂交(FISH)技术,以白缘(鱼央)的5S rDNA序列和小麦的45S rDNA为探针,对其在白缘(鱼央)雌雄中期染色体上进行了FISH定位研究。结果表明,白缘(鱼央)5S rDNA序列无雌雄差异;5S rDNA的保守区序列为117 bp;5S rDNA和45S rDNA分别被定位于白缘(鱼央)的性染色体和第5号染色体上。同时从GenBank中获得了22种鱼的5S rDNA,运用DNAman软件构建了23种鱼的系统发育树,对白缘(鱼央)的进化地位进行了初步研究。本研究为阐明白缘(鱼央)在鱼类系统进化中的位置、重复序列在脊椎动物性染色体上的分布状况以及与性别决定与分化的关系,提供了资料积累和分析依据。     

CMIP5模式对中国近海海表温度的模拟及预估

基于观测和再分析资料;利用多种指标和方法评估了国际耦合模式比较计划（CMIP5）中21个模式对中国近海海温的月、季节和年际变化模拟能力。多模式集合能够再现气候平均意义下近海海温的空间分布特征;但量值上存在一定的低估。在渤海和黄海;集合平均与观测差别比较明显。在年际尺度上;与观测数据对比;模式模拟海温与Niño3指数相关性较小。中国近海海表面温度在1960-2002年有明显的升高趋势;从2003年开始增温趋缓。评估结果表明;ACCESS1.0、BCC-CSM1.1、HadGEM2-ES、IPSL-CM5A-MR、CMCC-CM、FGOALS-g2、CNRM-CM5-2、INMCM4八个模式对中国近海海温的变化有较好的模拟能力。利用ACCESS1.0、INMCM4、BCC-CSM1.1、IPSL-CM5A-MR、CMCC-CM这5个模式结果对中国近海海温未来的变化进行了预估。在RCP4.5、RCP8.5情景下;未来近100年中国近海海温有明显升高趋势;最优模式多模式集合平均增温分别可达到1.5℃、3.3℃;净热通量变化和平流变化共同促进了东海升温。     

CMIP5地球系统模式溶解氧模拟结果分析

本文基于常用的统计方法,通过与WOA09观测的海洋溶解氧浓度数据进行比较,定量地评估了9个CMIP5地球系统模式在历史排放试验中海洋溶解氧气候态特征的模拟能力。在海表,由于地球系统模式均能很好地模拟海表温度（SST）,模式模拟的海表溶解氧浓度分布与观测一致,模拟结果无论是全球平均浓度偏差还是均方根误差均接近0,空间相关系数与标准偏差接近1。在海洋中层以及深层这些重要水团所在的区域,各模式的模拟能力则差异较大,尤其在溶解氧低值区（OMZs）所在的500m到1000m,各模式均出现全球平均偏差、均方根误差的极大值以及空间相关系数的极小值。在海洋内部,模式偏差的原因比较复杂。经向翻转环流和颗粒有机碳通量均对模式的偏差有贡献。分析结果表明物理场偏差对溶解氧偏差的贡献较大。一些重要水团,比如北大西洋深水,南极底层水以及北太平洋中层水在极大程度上影响了溶解氧在这些海区的分布。需要指出的是,虽然在海洋内部各模式模拟的溶解氧浓度偏差较大,但是多模式平均结果却能表现出与观测较好的一致性。     

放养密度对大杂交鲟生长性能的影响及生理应答机制

为探讨放养密度对鱼类生长及生理应答机制的影响规律和作用,作者以大规格大杂交鲟(达氏鳇(Huso dauricus)♀×施氏鲟(Acipenser schrenckii)♂)为实验材料,研究了平均初始体质量约为243 g/尾,放养密度分别为6(SD1组)、9(SD2组)、12(SD3组)、15 kg/m3(SD4组)条件下,不同放养密度处理70 d后的实验鱼生长性能变化及生理应答机制。结果显示,放养密度对大杂交鲟肥满度影响不显著,SD2组鱼类具有最大的特定生长率和生长效率,随着放养密度增加,日增质量显著降低(P0.05),特定生长率和生长效率下降。测定了血液甲状腺素(T4)、三碘甲腺原氨酸(T3)和皮质醇水平变化,发现放养密度能引起大杂交鲟3个血液生理指标发生显著改变;随着养殖时间推移,T3和皮质醇浓度显著升高,T4浓度显著下降(P0.05)。这些结果说明神经内分泌活动的变化引起大杂交鲟血液生理指标变化,进而影响实验鱼生长性能。因此,在该养殖条件下推荐的养殖密度为9 kg/m3。     

不同饵料种类、投喂方式及环境条件对凡纳滨对虾相对摄食量的影响

为提高对虾养殖过程中的饵料利用率并减少养殖废水的排放,作者以凡纳滨对虾(Litopenaeus vannamei)为实验对象,研究了不同饵料种类、投喂方式、体质量、充气量、光照强度、水温及盐度对其相对摄食量的影响。结果表明:配合饲料组对虾的相对摄食量显著高于冰鲜虾肉组;连续单颗投喂时对虾的相对摄食量较高;相对摄食量随对对虾体重升高而显著(P0.01)下降,而且不同规格的对虾在竞争条件下平均相对摄食量会降低;充气量6 L/min组对虾的相对摄食量明显高于另外两组;弱光环境下对虾的相对摄食量较高;水温和盐度对相对摄食量的影响极显著(P0.01),在32℃时对虾获得最大相对摄食量,在盐度为5时相对摄食量最小,高盐度下组间差异不显著(P0.05)。因此,在实际生产中应采取少量多次的投饵策略,并根据环境条件的变化合理的调整投饵量。     

Prediction of Seaward Slope Recession in Berm Breakwaters Using M5' Machine Learning Approach

In the design process of berm breakwaters, their front slope recession has an inevitable rule in large number of model tests, and this parameter being studied. This research draws its data from Moghim’s and Shekari’s experiment results. These experiments consist of two different 2D model tests in two wave flumes, in which the berm recession to different sea state and structural parameters have been studied. Irregular waves with a JONSWAP spectrum were used in both test series. A total of 412 test results were used to cover the impact of sea state conditions such as wave height, wave period, storm duration and water depth at the toe of the structure, and structural parameters such as berm elevation from still water level, berm width and stone diameter on berm recession parameters. In this paper, a new set of equations for berm recession is derived using the M5'' model tree as a machine learning approach. A comparison is made between the estimations by the new formula and the formulae recently given by other researchers to show the preference of new M5'' approach.     

Intercomparison of CRA-Interim Precipitation Products with ERA5 and JRA-55

By using the hourly data from surface meteorological stations in China, the 3-hour precipitation data from CRA-Interim (Chinese Reanalysis-Interim), ERA5 (ECMWF Reanalysis 5) and JRA-55 (Japanese Reanalysis-55) are compared, both on the spatial-temporal distributions and on bias with observation precipitation in China. The results show that: (1) The three sets of reanalysis datasets can all reflect the basic spatial distribution characteristics of annual average precipitation in China. The simulation of topographic forced precipitation in complex terrain by CRA-interim is more detailed, while CRA-interim has larger negative bias in central and East China, and larger positive bias in southwest China. (2) In terms of seasonal precipitation, the three sets of reanalysis datasets overestimate the precipitation in the heavy rainfall zone of spring and summer, especially in southwest China. CRA interim’s location of the rain belt in the First Rainy Season in South China is west by south, the summer precipitation has positive bias in southwest and South China. (3) All of the reanalysis datasets can basically reflect the distribution difference of inter-annual variation of drought and flood, but the overall the CRA-Interim generally shows negative bias, while the ERA5 and JRA-55 exhibit positive bias. (4) For the diurnal variation of precipitation in summer, all the reanalysis datasets perform better in simulating the daytime precipitation than in the night, and bias of CRA-interim is less in southeast and northeast than elsewhere. (5) ERA5 generally performs the best on the evaluation of quantitative precipitation forecast, the JRA-55 is the next, followed by the CRA-Interim. CRA-Interim has higher missing rate and lower threat score for heavy rains; however, at the level of downpour, the CRA-Interim performs slightly better.     

模式内部变率引起的1.5℃和2℃升温阈值出现时间模拟的不确定性研究

模式内部变率是模拟结果不确定性的重要来源,然而它对于1.5℃和2℃升温阈值出现时间不确定性的影响尚不清楚。因此,基于耦合模式比较计划第五阶段(CMIP5)的多模式数据研究了模式内部变率对1.5℃和2℃升温阈值出现时间不确定性的影响以及对未来排放情景的敏感性。结果表明,模式内部变率对升温阈值出现时间模拟的影响与外强迫的影响相当,单个模式内部不同成员达到全球平均1.5℃或2℃增温的年份相差2~12年;其影响具有明显的空间差异,影响极大值出现在欧亚大陆以北洋面、白令海峡周围区域、北美东北部及其与格陵兰岛之间的海域、南半球高纬地区等;低排放情景下模式内部变率的影响大于高排放情景。     

Response of ocean dynamics to multiple equilibria of the Kuroshio path South of Japan

Variability of the Kuroshio path to the south of Japan plays a central role in the local climate change and exerts tremendous influences on the local atmosphere and ocean. In this study, the response of ocean dynamics, in terms of the eddy kinetic energy (EKE), potential vorticity (PV), relative vorticity, and eddy-mean flow interaction, to the Kuroshio path change is discussed. Kuroshio path south of Japan includes the near-shore non-large meander (nNLM), the off-shore non-large meander (oNLM), and the typical large meander (tLM). Analyses reveal that the distribution of EKE, PV, relative vorticity, and energy exchange between the eddy field and the mean flow respectively varies with the Kuroshio path: (1) The tLM has the maximum EKE along the path; (2) The positive and negative PV are located at the onshore and offshore side of Kuroshio axis, respevetively; (3) The distributions of anomalous relative voritcity of nNLM, oNLM, and tLM are consistent with sea surface height anomalies (SSHAs); (4) The tLM has the largest energy exchange between the eddy field and the mean flow in terms of the rate of barotropic energy conversion. On the other hand, the stability analysis of ocean currents suggests that the three Kuroshio paths south of Japan have their own intrinsic properties of the instability.     

那曲高寒草地总体输送系数及地面热源特征

利用中国科学院那曲高寒气候环境观测研究站那曲/BJ观测点的野外观测数据,估算了青藏高原那曲地区典型高寒草地下垫面的热量和水汽总体输送系数以及地表大气相对湿度因子,在此基础上利用中国气象局那曲气象站1980-2016年的常规业务观测数据,采用总体输送法计算并分析了那曲高寒草地地表通量特征。研究结果表明:(1)那曲/BJ观测点地表大气相对湿度因子γ的数值在33%~62%,9月最大,2月最小,热量和水汽输送系数CH和Cλ的季节变化范围分别在1.6×10^-3~2.7×10^-3和1.0×10^-3~2.0×10^-3,两者存在较大的差异。(2)1980-2016年那曲高寒草地感热通量总体呈现减弱趋势,而潜热通量呈现增强趋势,导致地面热源变化趋势不明显;分阶段来看,感热通量的变化在2004年前后发生转折,转折点前后的趋势为先减弱后增加,潜热通量在1994-2005年下降趋势明显,这也导致地面热源在1995-2005年有一个明显的减少。(3)年内季节变化上潜热通量相较于感热通量更明显,地面热源的季节变化更依赖于潜热通量的季节变化。