草鱼出血病病毒VP7蛋白基因的人工合成与原核表达

根据GenBank已发表的GCRVVP7蛋白基因序列,设计合成2条特异性引物和19条搭桥引物,通过搭桥PCR人工合成带有3处变异碱基的GCRVVP7蛋白基因整个编码框,经过酶切、连接、PCR鉴定和测序鉴定克隆到原核表达载体pColdTF中,并转化大肠杆菌BL21(DE3),进行IPTG诱导表达和表达产物的SDS-PAGE、Western-blotting分析、纯化以及反应原性的iELISA检测。结果表明,成功人工合成了长831bp的GCRV VP7蛋白基因编码框和构建了VP7蛋白基因的重组质粒pCold TF-VP7,pColdTF-VP7转化菌经IPTG(1mmol/L)诱导3—4h即可获得特异性蛋白VP7重组蛋白的高效表达,VP7重组蛋白相对分子量大小约为73.9ku,与预期大小相符,且与鼠抗GCRV血清有良好反应原性。     

A new chronological framework for Middle and Upper Pleistocene landscape evolution in the Sussex/Hampshire Coastal Corridor, UK

The unique Middle and Late Pleistocene sedimentary record preserved along the Sussex/Hampshire Coastal Corridor between Romsey and Brighton contains a wealth of deposits including highstand marine sediments associated with a variety of different aged beaches, fluvial sediments associated with rivers crossing the coastal plain and cold stage deposits accumulating above the marine and fluvial sediments. Although quarrying activity has been extensive across much of the area it has been undertaken in flooded workings due to the high level of the watertable. Consequently little is known in detail about the sequences except where they outcrop on the foreshore around the coast. This paper examines recent work from the lower coastal plain using a multi-disciplinary approach these deposits to elucidate the age of the sequences and their associated environments of deposition.OSL dates from two of the beaches, the Aldingbourne and Brighton/Norton Beaches, place both within MIS 7. Although these OSL dates cannot differentiate between sub-stages within MIS 7, coupling these results with inferences from local geography, lithology and contained microfossils it is clear that the beaches belong to two different phases within MIS 7. These two beaches are clearly divided by a major phase of erosion and downcutting associated with a fall in sea-level. Fluvial sediments from Solent Terrace 2 and Arun Terrace 4 also date within MIS 7 and are tentatively ascribed to the downcutting event between the beaches. Together this information allows us to propose, for the first time, a robust independently dated framework for the lower parts of the coastal plain integrating for the first time the marine and terrestrial record.     

Evidence from the Trent terrace archive, Lincolnshire, UK, for lowland glaciation of Britain during the Middle and Late Pleistocene

The late Middle Pleistocene fluvial terrace sequence of the lower Trent system, Lincolnshire (eastern England), provides an excellent record of environmental change, including evidence for the last two interglacial episodes. It also provides important stratigraphical evidence for the timing and extent of three separate glaciations. Two of these, the Anglian and Devensian, are well-established correlatives of Marine Isotope Stages (MIS) 12 and 2 respectively; the third is a hitherto un-named post-Anglian-pre-Devensian glaciation in eastern England that has been the subject of much previous speculation, but can now be attributed with some confidence to MIS 8. Crucially, the recognition of MIS 7 interglacial deposits within the Balderton-Southrey terrace of the proto-Trent indicates that the underlying Wragby Till, which is ascribed to this additional glaciation, was emplaced no later than MIS 8. The oldest terrace preserved within the lower Trent staircase, the Eagle Moor-Martin Terrace, is considered to be a complex glacial outwash terrace related to the Wragby Till glaciation. It is suggested that deposits representing MIS 11-9, which are conspicuously absent throughout the Trent system, were removed by this glaciation. This is a departure from previous interpretations, which have suggested MIS 10 or MIS 6 as the most likely stages in which an extensive post-Anglian-pre-Devensian lowland glaciation might have occurred in Britain. However, the widespread preservation of undisrupted post-MIS 8 fluvial sequences throughout the Trent valley and in neighbouring systems, within which MIS 7 interglacial deposits have now been recognized at a number of localities, indicates that ice sheets are unlikely to have advanced further into this catchment during MIS 6 than during the Devensian (MIS 2). Recognition of a British glaciation during MIS 8 corresponds with widespread evidence in Europe, which suggests that glacial deposits classified as ‘Saalian’ represent both MIS 8 and MIS 6; in many areas, distinguishing these remains controversial, as confident correlation with either stage is often only possible where glacial sediments interdigitate with well-constrained fluvial records.     

添加剂对KAlSi_3O_8-CaSO_4-CaCO_3体系反应表观活化能的影响

首先测定了KAlSi3O8-CaSO4-CaCO3体系在不同焙烧条件下钾长石转化成可溶性氧化钾的转化率,进而通过模拟计算表明:KAlSi3O8-CaSO4-CaCO3体系的固相扩散动力学过程符合金斯特林格动力学方程,反应表观活化能Ea为128.92kJ/mol。并着重考察了4种添加剂Na2SO4、Na2SO3、NaCl和NaF对KAlSi3O8-CaSO4-CaCO3体系的影响,结果表明:向体系中分别加入占反应物总质量3%的Na2SO4、Na2SO3、NaCl和1%的NaF后,体系的反应表观活化能Ea从原来的128.92kJ/mol依次下降至87.15、98.71、117.38和126.14kJ/mol,这表明4种添加剂中Na2SO4的效果最好,它能较大程度上降低反应表观活化能,从而降低反应温度和提高固相反应速率。     

四川盆地白马地区白马8井垂向地球化学特征

文章在详细介绍各项油气化探指标异常形成机制的基础上,通过对白马8井不同地层中化探指标的变化规律、含量特征以及相关元素对比值分析,结果发现白马8井各层位均无油气富集,但发现须家河组第二和第四岩性段是区域上良好的储层,在今后的油气勘查中应该予以重视。     

210Pb and 7Be as Tracers for Aerosol Transfers at Center Guizhou，China：Ⅰ．The Explanation by Weekly Interval

It′s essential to measure air concentrations and depositional fluxes of environmental radionuclides in different regions for modeling global contamination transfer and validation, tracing deposition process and dating, referencing watershed erosion trace and assessing natural radiation of ecosystem. This study introduces the research background and methods and explains weekly variations of ²¹⁰Pb and ⁷Be concentrations in surface air and precipitation at Mt. Guanfeng/Guiyang since 2001. The results indicate that ²¹⁰Pb and ⁷Be concentrations in surface air fluctuate periodically at 2~6 week interval because of the meteorological condition changes. The annual trend in ²¹⁰Pb air concentration is overall influenced by air temperature variation and periodical rainfall. ⁷Be air concentration varies up and down regualarly. The synchronization of low concentrations for both ²¹⁰Pb and ⁷Be in surface air and high ratio (²¹⁰Pb/⁷Be ≥ 0.8) indicate oceanic poor ²¹⁰Pb-⁷Be air mass invasion. The synchronization of high concentrations of ²¹⁰Pb and ⁷Be in surface air and low ratio (²¹⁰Pb/⁷Be ≤ 0.7) implies rich ⁷Be air mass invasion from altostratus atmosphere. Weekly concentrations of ²¹⁰Pb and ⁷Be in precipitation and the K_d coefficients are high in late Autumn to Spring, and low in late Spring to Autumn. Although ²¹⁰Pb and ⁷Be concentrations vary seasonally, their weekly concentrations in precipitation increase with that of air concentration. The ²¹⁰Pb and ⁷Be weekly concentrations for seasonal division is about 0.2 Bq/L and 1 Bq/L, respectively. However, the K_d coefficient decreases when ²¹⁰Pb and ⁷Be air concentrations increase. Deposition of ²¹⁰Pb and ⁷Be with precipitation mainly take place in Spring and Summer; The weekly fallout of ²¹⁰Pb and ⁷Be increases with increasing precipitation; the increase of ⁷Be is triple of that of ²¹⁰Pb and 2.5 when precipitation nears to zero. The weekly-high fallout of ²¹⁰Pb and ⁷Be with precipitation corresponds with the dramatic decrease of their air concentrations within or vicinal weeks. The ratio of ²¹⁰Pb to ⁷Be in rainfall is overall ≤ 0.5 and varies synchronously with the ratio in surface air, indicating that the two radionuclides are cleaned by precipitation from air at close speed. In the extreme freezing disaster in 2008, both ²¹⁰Pb and ⁷Be air concentrations were low and coupling with coexist of low concentration of aerosol and high nuclides activities. Weekly concentration of ⁷Be in surface air at Mt. Guanfeng demonstrates the anticipated concentration level in the low-latitude and high-elevation region; the short-term fluctuations of ⁷Be concentration indicated the hysteresis was influcenced by the strong air current submersion and the low-elevation air mass rising differently at Mt. Guanfeng and Mt. Waliguan.      

汶川8.0级大地震震源机制与构造运动特征

根据地震震源机制、断层参数结果,结合GPS测定的同震位移场与构造研究的最新结果,综合分析研究了2008年汶川8级大地震汶川地震发生的地震活动背景、震源应力场、断层构造运动特征及其动力学机制。地震活动性分析研究结果表明,2008年汶川8级大地震是在青藏高原与其周边地域构造运动剧烈,2001年起始的地震活动高潮期的背景下发生的。其长达300km的地震震源断层填补了青藏高原东缘1900年以来存在的8级地震活动的空区。震源机制与区域应力场特征及其动力学机制研究表明,汶川8级地震震源处于南北地震带中南段东部,青藏高原东向扩张与四川盆地的抵抗是该区构造运动的主要特征。汶川地震及其强余震是在一个稳定的、主压应力P轴以北西西-东南东方向为主的震源应力场控制下发生的。说明汶川地震震源区域主要受到四川盆地、华南块体区域应力场的控制并发震的。龙门山断裂带西侧的青藏高原相对于四川盆地发生的东向上升;而东侧的四川盆地相对于青藏高原发生的西向下降构造运动是2008年汶川8级地震发生的主要地震成因即地震发生机制。     

基于MicroStation V8的地形图更新与质检系统的开发

在MicroStation VBA环境下,对MicroStation V8进行二次开发,实现了一套基于MicroStation V8平台的多比例尺地形图要素更新与质检系统。该文着重阐述了该系统在处理点、线、面、注记、属性、出图效果及质量检查控制等方面的设计。通过二次开发,快速实现了地形图的更新与维护,并有利于质量的控制,符合入库要求。     

Effects of substratum type on fish assemblages in shallow areas of a tropical estuary

While there is already a comprehensive understanding of the effects of environmental variables, such as dissolved oxygen, temperature and salinity, on the structure, biomass and metabolism of aquatic biota in estuarine habitats, the effect of sedimentation, a harmful underlying factor, remains unclear. The aim of this study was to assess the differences in fish assemblages along the freshwater to salt water gradient of a large tropical estuary, and to evaluate the effects on them of habitat disturbance associated with shallow water sedimentation in the intertidal areas. Fish and environmental variables were recorded in the upper, middle and lower salinity zones of the estuary at ebb tide in both the dry and wet seasons. Sediment samples associated with different levels of habitat disturbance were analysed using granulometry, and their organic content and dissolved oxygen levels were quantified. Water temperature, salinity, pH and dissolved oxygen levels were also measured. Habitat disturbance was found to be correlated with the density, biomass and richness of fish assemblages. A total of 77 species were recorded, forming two distinct fish assemblages, with the Eleotridae family dominating in the upper zone, and Gerreidae, Gobiidae and Tetraodontidae the most common in the middle and lower estuary. Changes in the structure of fish assemblages, including reductions in density, biomass and richness, were associated with disturbance to natural features, where muddy sediment was replaced by sandy sediment and the quantity of organic matter fell. Atherinella brasiliensis was the species which showed a preference for the disturbed areas in the middle and lower zones, while Dormitator maculatus showed a preference for them in the upper estuary. They may be taken as indicators of habitat disturbance due to shallow sedimentation.