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Mingyi WANG Xiaobing LIANG Yaping ZHENG Youzhi ZHAO Zhongqing WEI 《中国地球化学学报》2006,25(B08):65-66
The purpose of the thesis is to analyze the temporal and dimensional distribution of sulfate-reducing bacteria (SRB) groups and quantity in Lake Erhai. In April and September 2005, two sediment cores were collected from Lake Erhai. SRB groups were analyzed by PCR with six-groups primers designed according to the specific 16SrDNA sequence. FISH (fluorescence in-situ hybridization) was established with the oligonucleotide probe (SRB385) and utilized to analyze SRB quantity in the sediments. The results showed that in the sediments of Lake Erhai four SRB groups were detected except Desulfobacterium and Desulfobacter, meanwhile Desulfovibrio-Desulfomicrobium were detected only in autumn; different SRB groups had different temporal and dimensional distribution, and each group in autumn is distributed more widely than in spring; FISH used to count SRB in the sediments of fresh lake was set up successfully; the analysis of correlation between the sediment's depth and SRB quantity had statistical meaning (P〈0.05) . The result showed that SRB quantity showed a decreasing trend with increasing depth. Through the analysis of randomized block designed analysis of variance, the difference in SRB quantity between spring and autumn also had statistical meaning (P〈0.001), which revealed SRB quantity in autumn was larger than in spring; the result of FISH showed that there were some SRB in the deeper sediments in which no above-mentioned six SRB groups were detected by PCR. SRB groups in the sediments of Lake Erhai were rich, and the quantities of SRB groups in autumn were larger than in spring; possibly there were uncultivable SRB groups in the sediments of Lake Erhai. 相似文献
44.
Lichun LIU Yuting CHEN Kuanyu CHEN Jyhyih LEU Tsungkwei LIU 《中国地球化学学报》2006,25(B08):67-67
High concentrations of arsenic and humic substances in groundwater from the southwestern coastal plain of Taiwan were well known for their probable relationships with black-foot disease. In order to realize the relationships between the concentrations of humic substances and arsenic in this area, 24 well water samples were analyzed. After filtered through 0.45 μm glass fiber membrane in the field, samples were kept in the dark at 4℃ and then separated into six fractions with varying range of molecular weight (〈0.5, 0.5-1, 1-5, 5-10, 10-50 and 〉50 k Da) by ultrafiltration apparatus (Molecular/Por Stirred Cell system) in the lab. Concentrations of humic substances were measured by fluorescence spectrometer (HITACHI F-2000, ex=370, em=445) and arsenic by FIAS-AA (Perkin Elmer AAnalyst 100, FIAS-400). On average, only 6.2% of the total arsenic in water existed in the fraction of 〉0.5 k Da. and the others were complexed with humic substances (including humic acid and fulvic acid). The results demonstrated a distinct positive correlation between the concentrations of humic substances and arsenic. 相似文献
45.
Zhengji YI Kaixuan TAN Zhenxun YU Aili TAN Shiqiang WANG 《中国地球化学学报》2006,25(B08):125-126
Uranium processing and mining activities that generate many contaminants, such as high concentrations of U (VI), sulfate and heavy metals (Zn, Cu, Ni, etc), may pose a serious threat to the groundwater resources. In recent years, considerable research has been conducted respectively on two kinds of permeable reactive barriers (PRB), including zerovalent iron (ZVI) and sulfate reducing bacteria (SRB), for in-situ removal of these pollutants from groundwater. However, little investigation has been carried out on the potential benefits of bioaugmenting ZVI barriers to enhance the elimination of the pollutants by combining ZVI with SRB systems. The main goal of this study was to conduct batch and column experiments to determine whether the combination of SRB and ZVI can function synergistically and accelerate the rate of pollutant removal. The results of anaerobic batch experiments demonstrated that although the integrated ZVI/PRB system itself has no ability to reduce and remove sulfate directly, SRB can utilize hydrogen gas produced during the slow process of ZVI corrosion as an electron donor to raise biomass yields significantly and accelerate reductive sulfate removal. In particular, ferrous cations produced as the byproduct of ZVI corrosion process reacted with hydrogen sulfide from sulfate reduction and formed iron-bearing sulfide precipitates, which can stimulate the growth of SRB and promote sulfate removal activity by eliminating the biotoxicity of hydrogen sulfide. It was also shown that secondary mineral products (pyrite/ferrous sulfide) formed as a consequence of microbial sulfate reduction and ZVI corrosion process can enhance the microbial precipitation of soluble U (VI) as insoluble uraninite(uranium dioxide). 相似文献
46.
Christina A. Kellogg 《中国地球化学学报》2006,25(B08):218-219
Desert winds aerosolize several billion tons of soil-derived dust each year, including concentrated seasonal pulses from Africa and Asia. Huge dust events create an atmospheric bridge over continents and oceans, and eject a large pulse of soil-associated microorganisms into the atmosphere. These dust events might therefore have a role in expanding the biogeographical range of some microorganisms by facilitating rare long-distance dispersal events. The goal of this study is to characterize the microbes associated with African dust events and determine if they pose a risk to humans or downwind ecosystems. Air samples were collected by vacuum filtration in a source region (Mali, West Africa) during dust events and plated on R2A media to culture microorganisms. These organisms were compared to those in similar samples collected in the Caribbean during Saharan/Sahelian dust events. A high-volume liquid impinger is currently being tested in Barbados, collecting aerosol samples during African dust events. Over 100 bacteria and fungi (19 genera of bacteria and three genera of fungi) have been characterized from source region dust events. 相似文献
47.
本文对影响南极细菌S-15-13生长和胞外多糖产量的主要环境因子进行了研究,同时采用16S rDNA序列分析及系统发育分析方法对其进行了分子鉴定。结果表明:菌株S-15-13最佳产糖条件为:培养时间,56h;培养温度,8℃;碳源,1.0%葡萄糖;NaCl浓度,3.0%;pH,6.0-7.0。16S rDNA序列分析和系统发育分析表明,菌株S-15-13属于假交替单胞菌属(Pseudoaltero- monas)。 相似文献
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广西凤凰山锰银氧化矿可选性试验研究 总被引:3,自引:0,他引:3
锰银氧化矿是公认的难选冶矿石类型,在当前经济技术条件下仍属于难以利用的矿种。采用强磁选方法优先回收银锰、磁选尾矿氰化浸银的试验流程,获得银回收率达到90%的良好效果。 相似文献
50.
覆盖在北极海洋上的广阔海冰为其内部生存的微生物群落提供了一个独特的生境。研究表明 ,大量海冰细菌能够分泌产生胞外酶 ,其中产蛋白或脂质水解酶细菌的比例远高于产多糖水解酶的细菌。温度、盐度是直接影响海冰细菌生存与活力的 2个主要环境因子。 76%的产酪蛋白酶海冰细菌为低温菌 ,菌株只能在 <35°C条件下生长 ;而 98%的酪蛋白酶最适作用温度≥ 35°C ,其中 62 %的酶最适作用温度≥ 45°C。几乎所有的产酪蛋白酶海冰细菌都耐盐或嗜盐 相似文献