大西洋鲑在封闭循环水养殖模式下的生长和性腺发育研究

本研究探讨了在封闭循环水养殖模式下大西洋鲑(Salmosalar)的生长发育模式,对封闭循环水系统养殖的大西洋鲑重要生长数量性状进行生物学测量,并跟踪性腺发育状况。本研究自2012年1月通过封闭循环水系统进行大西洋鲑养殖,经过周年养殖,体质量平均达到1194.42g,体长平均达到417.00 mm,体高平均达到100.58 mm,体宽平均达到53.52 mm,少数个体在养殖10个月后性腺发育较快,性腺质量达到9.76 g,性腺指数达到1.31%。本研究的研究结果为建立大西洋鲑标准化循环水养殖系统积累资料。     

长山群岛海洋渔业资源的平均营养级变化特征

根据长山群岛 1965-2016 年渔业统计资料,分析长山群岛海域主要捕捞渔获物产量、平均营养级 （Mean trophic level, MTL）、渔业均衡指数 （Fishing in balance index,FiB） 年际变化,探讨其海洋渔业资源利用状况,并利用小波分析方法研究52年来渔获物 MTL 周期变化特征。研究表明： （1） 长山群岛捕捞产量、MTL 和 FiB 指数呈阶段性变化; （2） 长山群岛渔业资源开发经历初期开发、扩张捕捞、过度捕捞、资源破坏等四个阶段,渔业资源环境正在逐渐恶化;（3） 受人类捕捞活动影响,MTL 在 15~19 年和 24~34 年两种时间尺度下呈周期波动,30 年为第一主周期,17 年为第二主周期。长山群岛渔业资源破坏日益严重,未来几年平均营养级将呈下降趋势。为防止渔业资源进一步衰退,应加强捕捞活动管理力度,落实海洋渔业资源保护制度;完善预警机制,构建海洋渔业资源监测系统;同时应积极调整长山群岛渔业产业结构,提高资源产出效率.     

基于Argo浮标的西太平洋SMAP卫星海表面盐度数据质量评估

以西太平洋为研究区域,利用Argo浮标表层盐度观测值(5 m)对SMAP卫星获得的2016年海表面盐度反演质量进行了评估。首先将西太平洋2016-01—12期间的每日和每月SMAP卫星SSS数据与Argo实测SSS数据进行匹配,然后利用最小二乘线性回归法对其进行相关性分析,并对误差的分布特征进行了研究。结果表明:SMAP SSS与Argo SSS之间具有极显著的正相关关系;每日Argo浮标数据(WMO ID:2901520,WMO ID:2901548)和SMAP SSS的变化趋势基本一致,前者均方根误差(RMSE)、偏差(Bias)和相关系数(r)分别为0.43, 0.34和0.71,后者RMSE,Bias和r分别为0.41,0.26和0.69;研究区域内全年RMSE值处于0～0.35,在西太平洋南部海域偏差较大,这可能是由于该海域小岛众多,缺少Argo实测数据,导致其网格化的盐度存在较大误差。除夏季外,研究区域的大部分海域,RMSE都小于0.25。在海表盐度较低的海域,两者的对比结果误差较大,该现象在夏秋两季尤为显著。     

《应用海洋学学报》征稿简则

《应用海洋学学报》是国家海洋局第三海洋研究所、中国海洋学会、福建省海洋学会主办,中国生态学学会海洋生态专业委员会协办,国家海洋局主管,国内外公开发行的全国性综合性海洋科学学报(季刊,国内统一刊号:CN35-1319/P,国际标准刊号:ISSN2095-4972)。《应用海洋学学报》是中文核心期刊、中国科技核心期刊、中国科学引文数据库(CSCD)收录期刊、中国学术期刊(光盘版)收录期刊和中国核心期刊(遴选)数据库收录期刊,也是中国、美国、英国、日本以及联合国等十几个科技期刊数据库的收录期刊。     

Contribution of trimethylamine N-oxide on the growth and pressure tolerance of deep-sea bacteria

Trimethylamine N-oxide(TMAO) is widely dispersed in marine environments and plays an important role in the biogeochemical cycle of nitrogen. Diverse marine bacteria utilize TMAO as carbon and nitrogen sources or as electron acceptor in anaerobic respiration. Alteration of respiratory component according to the pressure is a common trait of deep-sea bacteria. Deep-sea bacteria from dif ferent genera harbor high hydrostatic pressure(HHP) inducible TMAO reductases that are assumed to be constitutively expressed in the deep-sea piezosphere and facilitating quick reaction to TMAO released from ?sh which is a potential nutrient for bacterial growth. However, whether deep-sea bacteria universally employ this strategy remains unknown. In this study, 237 bacterial strains affliated to 23 genera of Proteobacteria,Bacteroidetes, Firmicutes and Actinobacteria were isolated from seawater, sediment or amphipods collected at dif ferent depths. The pressure tolerance and the utilization of TMAO were examined in 74 strains. The results demonstrated no apparent correlation between the depth where the bacteria inhabit and their pressure tolerance, regarding to our samples. Several deep-sea strains from the genera of Alteromonas, Halomonas,Marinobacter, Photobacterium, and Vibrio showed capacity of TMAO utilization, but none of the isolated Acinebacter, Bacillus, Brevundimonas, Muricauda, Novosphingobium, Rheinheimera, Sphingobium and Stenotrophomonas did, indicating the utilization of TMAO is a species-speci?c feature. Furthermore, we noticed that the ability of TMAO utilization varied among strains of the same species. TMAO has greater impact on the growth of deep-sea isolates of Vibrio neocaledonicus than shallow-water isolates. Taken together, the results describe for the ?rst time the TMAO utilization in deep-sea bacterial strains, and expand our understanding of the physiological characteristic of marine bacteria.     

Primary molecular basis of androgenic gland endocrine sex regulation revealed by transcriptome analysis in Eriocheir sinensis

In crustaceans, the male sexual dif ferentiation and maintenance are specially regulated by androgenic gland(AG). However, little is known about the genes involved in the regulation process.RNA-Seq was performed on AG with ejaculatory duct(AG_ED) and ejaculatory duct(ED) as control in Eriocheir sinensis, one of the most important economic and ?shery crabs with typically sex dimorphism. A total of 925 unigenes were identi?ed as dif ferentially expressed genes(DEGs) and the expression of nine genes randomly selected was con?rmed by qRT-PCR. 667 unigenes were up-regulated in AG_ED, being supposed to be AG preferential genes. Among them, the full length of i nsulin-like androgenic gland factor( IAG) cDNA named as Es-IAG was obtained as a logo gene of AG, which together with the genes i nsulin-like receptor( INR), and s ingle insulin binding domain protein( SIBD), might constitute the sex regulation pathway. Several sex related genes were identi?ed, and their function will have to be investigated. Also,the identi?cation of j uvenile hormone epoxide hydrolase 1( JHEH1), ecdysteroid 22-hydroxylase( DIB) and e cdysone receptor( ECR) preliminarily clari?ed the molecular regulation mechanism of eyestalk-AG-testis axis, which plays important roles in molting and reproduction. The results will enhance our understanding for the molecular basis of the AG involved in male sex regulation in crabs.     

Dynamic metabolite alterations of Portunus trituberculatus during larval development

A mass mortality often occurs from molting to the megalopa stage during the larval development of the swimming crab Portunus trituberculatus. Larvae with insuffcient nutrient accumulation during the zoeal stages are probably unable to develop into juvenile swimming crabs. However, the nutritional information such as the primary metabolites is scarce for P. trituberculatus larvae. The aim of this work is to obtain an insight into the metabolite traits of P. trituberculatus at early developmental stages. 1 H nuclear magnetic resonance spectroscopy coupled with multivariate data analysis was used to determine how the metabolite pro?les shift during larval development in P. trituberculatus. Our results show that the trend of total metabolites exhibited a rise from zoea 1 to zoea 3, followed by a drop from zoea 4 to megalopa and recovery during the ?rst juvenile stage. A large-scale depletion of total metabolites in the zoea 4 and megalopa stages suggests a deep depression of metabolic activity, which may be linked to the mass mortality from molting to the megalopa stage. These ?ndings provided essential metabolic information about the larval development of P. trituberculatus and important clues for understanding the nutritional requirements of P. trituberculatus in early developmental stages.     

An integrated approach to assess the ecological and chemical status of lakes with HOD/AHOD: a case study of two lakes

The thermal strati?cation of the lakes impedes the transfer of atmospheric oxygen into the lower layers of the lake. In lakes which are af fected by diverse anthropogenic in?uences, the increasing organic matter amounts lead to a sharp decrease in hypolimnetic oxygen amounts, aided by thermal strati?cation,and anaerobic conditions arise. The determination of hypolimnetic oxygen demand(HOD) and areal hypolimnetic oxygen demand(AHOD) and their monitoring represent an integrated approach to investigate the oxygenation of lakes, the nutrient conditions, and the physicochemical dynamics. In this study, two lakes dif fering by size and af fected by dif ferent anthropogenic sources, are investigated in this respect. At?rst, bathymetric studies were conducted to determine the depth, surface area, and volume relationships.Then, based on monitoring studies conducted in 2013 and 2014, the thermal strati?cation dynamics and layer properties were established using the relative thermal resistance to mixing(RTRM) index based on temperature and density pro?les. Following this, the oxygen depletion rates were determined by oxygen and temperature pro?ling in the hypolimnion. For the years of 2013 and 2014, the AHOD values for the Borabey Pond which is far from anthropogenic in?uences, were found to be 0.848 and 0.569 g O 2/(m·d), respectively.The AHOD values for the Porsuk Reservoir which was overburdened for years by industrial and domestic pollution were found to be 4.263 and 5.099 g O 2/(m·d), larger than its counterpart by almost sevenfold. The HOD and AHOD monitoring can be considered to a valuable tool for assessing the ecological and chemical status of lakes within Annex 5 of the Water Framework Directive and as an integrated approach to assess and monitor the status of lakes.     

Verification and recalibration of HY-2A microwave radiometer brightness temperature

HY-2A is the first one of the Chinese HY-2 ocean satellite series carrying a microwave radiometer(RM)to measure sea surface temperature,sea surface wind speed,atmospheric water vapor,cloud liquid water content,and rain rate.We verified the RM level 1B brightness temperature(T B)to retrieve environmental parameters.In the verification,TB that simulated using the ocean-atmosphere radiative transfer model(RTM)was used as a reference.The total bias and total standard deviation(SD)of the RM level 1B TB,with reference to the RTM simulation,ranged-20.6-4.38 K and 0.7-2.93 K,respectively.We found that both the total bias and the total SD depend on the frequency and polarization,although the values for ascending and descending passes are different.In addition,substantial seasonal variation of the bias was found at all channels.The verification results indicate the RM has some problems regarding calibration,e.g.,correction of antenna spillover and antenna physical emission,especially for the 18.7-GHz channel.Based on error analyses,a statistical recalibration algorithm was designed and recalibration was performed for the RM level 1B TB.Validation of the recalibrated TB indicated that the quality of the recalibrated RM level 1B TB was improved significantly.The bias of the recalibrated T B at all channels was reduced to<0.4 K,seasonal variation was almost eradicated,and SD was diminished(i.e.,the SD of the 18.7-GHz channel was reduced by more than 0.5K).