Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.     

Issues concerning global warming today

The global weather of today is growing significantly warmer; this is an indisputable fact. However, the scientific community has not yet reached consensus on the causes of global warming and its possible consequences. This paper introduces the causes of global warming and summarizes its results, which both involve a series of huge and complex system issues. Our top priority is to pinpoint the main reason and the interrelated links between causative factors by adopting a macro-approach, or comprehensive comparison analysis. Its physical mechanism was then determined and its digital model established after quantitative study. __________ Translated from Green Leaf, 2007, (8): 34–35 [译自: 绿叶]     

地震数据IP传输及存储技术应用

介绍一种IP传输方案。该方案通过地震数据IP传输及存储单元(ITU)、IP流多串口服务器完成，能实现单个台站与多个台网中心的Internet传输、单个台站与多个台网中心的专线传输、地震台网中心之间的Internet连接、远程数据下载及参数设置等多种传输方式，该传输方案具备实时性、可压缩性、多路服务、安全性、数据在线保存、传输费用低、操作简单等特点。     

四川盆地碳酸盐岩油气勘探实践与认识

四川盆地烃源十分丰富。但油气演化程度高,大多已进入高成熟和过成熟阶段,至今只在侏罗系发现有油藏,三叠系及其更老地层皆为气藏。储层主要为碳酸盐岩,其特点是低孔低渗,平均孔隙度仅1.7%,平均渗透率小于1×10~(-3)μm~2,但其中的“孔洞发育层段”平均孔隙度可达3%～6%。根据四十年的勘探经验,加强预探并争取新发现是四川盆地油气勘探发展的关键。预探中,按构造带规划大中型气田勘探目标,将成藏条件相似、地域上相邻的局部圈闭群作为一个整体勘探对象,实行稀井广探,少井高效。“八五”以来,在高陡构造带勘探技术、储层横向预探技术等方面有长足的进步,勘探成效不断提高,实现了资源序列的良性循环。     

地质雷达快速检测运营公路隧道隐患

为了评价运行多年隧道的衬砌质量，查找隐患，开展了拱顶的地质雷达无损快速检测。在雷达波形剖面图上分析空洞、不密实异常，推断出大量隐患。物探成果经过开凿验证，证明其隐患定位成功率在85％以上。地质雷达快速检测方法为隧道病害治理提供了可靠的基础资料。     

碎石桩复合地基检测分析

结合南京钢铁集团新钢铁厂碎石桩复合地基工程实例，从桩身的密实性和连续性、桩体周围土的隆起量以及桩间土挤密效果3个方面对碎石桩复合地基进行检测分析。     

利用催化动力学原理测定化探样中微量碘的含量

在HAc-NaAc形成的中性缓冲溶液体系中,利用碘的催化动力学原理,使N-N-四甲基-4-4-二氨基二苯甲烷与氯胺T的氧化反应,生成蓝色络合物来测定地探样中微量碘的含量。通过实验,最佳试剂加入量为8%的HAc溶液5mL,8%的Na2CO3溶液3mL,0.1%的N-N-四甲基-4-4-二氨基二苯甲烷溶液5mL所组成的溶液体系中,在加入0.1%的氯胺T1mL显色后,其测定结果准确度、精密度高,检出限低,测定化探样中微量碘时该方法可以推广应用。     

北疆寒潮天气分析及预报

利用1998-2014年20场北疆典型寒潮天气过程的高空、地面和数值预报产品资料,对北疆寒潮天气进行了环流分型和区域分型,总结了环流型与冷空气路径、区域类型的对应关系,重点统计了不同季节北疆寒潮爆发前一天内500hPa脊前北风急流、锋区强度、中低空冷空气强度、地面冷高压等特征量的平均值和最大、最小值,并检验欧洲ECMWF数值预报产品在北疆寒潮天气预报中的预报能力,为北疆寒潮精细化预报提供了技术参考。     

FFT和DFT在地震数据采集器幅频特性测试中的应用

在进行地震数据采集器的幅频特性测试中,当标定信号频率稍高时(约为采集器采样率1/5以上),采集器输出的正弦波形发生畸变,无法人工读取正弦波峰值.为了解决该问题,本文提出了基于离散傅里叶变换(DFT)的积分恢复算法,将该方法与快速傅里叶变化(FFT)谱分析方法同时应用于一台高精度地震数据采集器的幅频特性测试数据处理.结果表明这两种算法均能标准化、高精度地完成采集器幅频特性标定数据的计算.其中基于DFT的积分恢复算法的计算程序操作简单,能够高效率地完成数据采集器的幅频特性检定工作;并且该方法还可以应用于地震数据采集器的线性度测试.     

金刚石选矿方法与评价

金刚石原生矿和砂矿的选矿方法各有异同,但总体来说可分为粗选和精选两个阶段.粗选主要是利用重选跳汰法来进行,精选采用多种方法联合进行.而质量则是整个选矿过程的关键,质量检查应采取多种方法进行,每个工序和各个级别有不同的质量要求.金刚石总的回收率应不低于95%.