Initiation of primary cell culture from amphioxus <Emphasis Type="Italic">Branchiostoma belcheri tsingtauense</Emphasis>

Amphioxus, a cephalochordate, is an important model fish for studies in evolution and comparative biology. A successful cell culture from amphioxus tissues in vitro would help understanding some basic issues. To determine the optimal culture conditions for proliferation of amphioxus cells, primary cultures were initiated from buccal cirri, tail, gill, gut and metapleural fold of amphioxus Branchiostoma belcheri tsingtauense. The media tested were L-15, F-12, M 199, MEM, DMEM, PRMI 1640 and LDF, each was supplemented with 20% fetal bovine serum. The optimal conditions include tail tissue cultured in L-15 or F-12 with supplement of 20% FBS and 1.5% NaCl at about 25°C. Supported by Doctoral Initial Fund of Ludong University (No.43304)     

Characterization of a new lectin involved in the protoplast regeneration of <Emphasis Type="Italic">Bryopsis hypnoides</Emphasis>

A group of coenocytic marine algae differs from higher plants,whose totipotency depends on an intact cell(or protoplast).Instead,this alga is able to aggregate its extruded protoplasm in sea water and generate new mature individuals.It is thought that lectins play a key role in the aggregation process.We purified a lectin associated with the aggregation of cell organelles in Bryopsis hypnoides.The lectin was ca.27 kDa with a pI between pH 5 and pH 6.The absence of carbohydrate suggested that the lectin was ...     

Characteristics of water exchange in the Luzon Strait during September 2006

The Luzon Strait is the only deep channel that connects the South China Sea(SCS) with the Pacific.The transport through the Luzon Strait is an important process influencing the circulation,heat and water budgets of the SCS.Early observations have suggested that water enters the SCS in winter but water inflow or outflow in summer is quite controversial.On the basis of hydrographic measurements from CTD along 120° E in the Luzon Strait during the period from September 18 to 20 in 2006,the characteristics of t...     

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冷岛效应的分析解

本文将冷岛视为一个负的扰动加热源,即冷源,用小扰动方法求出了冷岛效应的分析解,给出了解例,并与热岛效应的结果作了对比分析。     

Deriving Changjiang coastal zone wind from C-band SAR and its application to salinity simulation

Wind plays an important role in hydrodynamic processes such as the expansion of Changjiang （Yangtze） River Diluted Water （CDW）, and shelf circulation in the Changjiang estuary. Thus, it is essential to include wind in the numerical simulation of these phenomena. Synthetic aperture radar （SAR） with high resolution and wide spatial coverage is valuable for measuring spatially inhomogeneous ocean surface wind fields. We have collected 87 ERS-2 SAR images with wind-induced streaks that cover the Cbangjiang coastal area, to verify and improve the validity of wind direction retrieval using the 2D fast Fourier transform method. We then used these wind directions as inputs to derive SAR wind speeds using the C-band model. To demonstrate the applicability of the algorithms, we validated the SAR-retrieved wind fields using QuikSCAT measurements and the atmospheric Weather Research Forecasting model. In general, we found good agreement between the datasets, indicating the reliability and applicability of SAR- retrieved algorithms under different atmospheric conditions. We investigated the main error sources of this process, and conducted sensitivity analyses to estimate the wind speed errors caused by the effect of speckle, uncertainties in wind direction, and inaccuracies in the normalized radar cross section. Finally, we used the SAR-retrieved wind fields to simulate the salinity distribution off the Changjiang estuary. The findings of this study will be valuable for wind resource assessment and the development of future numerical ocean models based on SAR images.     

Pigment characterization for the 2011 bloom in Qinhuangdao implicated "brown tide" events in China

A large-scale bloom occurred from May to June in 2011 in sea area near Qinhuangdao of the Bohai Sea, leading to huge damage of the scallop culture industry. Similar blooms have been observed in this region for three years. The causative species of the bloom, which dominated the phytoplankton community with the maximum cell density around 109 cell/L, could not be identified with morphological features due to the small cell size (~2 mm). A pigment analytical method was then adopted to analyze the pigment profile of the phytoplankton samples collected from the blooming sea area. It was found that pico-sized (<2 mm), nano-sized (2-20 mm), and bulk phytoplankton samples had similar pigment profile, representing the pigment signature of the bloom-causative species. The major pigments detected included 19-butanoyloxyfucoxanthin (But-fuco), fucoxanthin (Fuco), diadinoxanthin (Diad) and chlorophyll a (Chl a), and high content of But-fuco was the most significant characteristics of the phytoplankton samples. Based on the pigment composition and content, the bloom-causative species could be tentatively identified as pelagophyte, "type 8" group of haptophyte, or silicoflagellate. Some unique features of the bloom, such as the extremely high cell density, small-sized and But-fuco containing cells, occurring in early summer, and the feeding-cessation effects on scallops, suggest it be a "brown tide" event similar to those reported in the east coast of the United States of America. The recurrent "brown tide" events and their dramatic impacts on the shellfish mariculture industry in Qinhuangdao need close attention in the coming years.     

Taxonomic distinctness of macrofauna as an ecological indicator in Laizhou Bay and adjacent waters

In this paper, we assessed the ecological and biodiversity status in the Bohai Sea through a quantitative survey on mac-rofaunal community at 25 stations in Laizhou Bay and adjacent waters in the autumn of 2006.We tested the robustness and effectiveness of taxonomic distinctness as an ecological indictor by analyzing its correlation with species richness and natural environmental variables and by analyzing other ecological indicators (Shannon-Wiener H' and W statistics from Abundance Biomass Comparison curve).Results so obtained indicated that the benthic environment of the study waters in general is not under major impact of anthropogenic disturbance, but some stations in Laizhou Bay and along the coast of the Shandong Peninsula and even in the central Bohai Sea might be moderately disturbed and showed signs of ecological degradation.The taxonomic distinctness measures △+ and Λ+ were independent of sampling effort and natural environment factors and were compliant to other ecological indicators.Further application of the taxonomic distinctness indicator to assess marine biodiversity and ecosystem health on a larger regional scale with historical data seems promising.     

取土器几何尺寸对饱和软黏土样结构的扰动研究

基于颗粒流软件模拟,以吹填淤泥为例,分析了取土器的内径、切角、壁厚等尺寸参数对高黏性软土微观动力学特性的影响;通过分层方法,比较了7组土样层间颗粒特征拐点的运动行为。模拟结果表明:土样的颗粒在底部形成对流位移场,对流处上方颗粒主要受拉膨胀,对流处下方颗粒主要受压收缩,由此造成孔隙率的规律变化和层理的弯曲变形。7组参数对应土样的孔隙率曲线与初始孔隙率曲线的交点同颗粒位移场的对流位置存在协同关系。土样层理表现为向下弯曲变形,层间颗粒位移行为存在特征拐点,拐点之间颗粒扰动行为不明显;反之,颗粒扰动行为较大,这种变形特征可拟合为下开口的旋转抛物面,且曲面方程的曲面形状拟合系数a值能初步评估7组土样的扰动程度。层间特征拐点的连线可再拟合为旋转抛物面,抛物面体积与土样体积的比值可用来评估土样的原状性。     

NUFFT-Based Iterative Reconstruction Algorithm for Synthetic Aperture Imaging Radiometers

Synthetic aperture imaging radiometers (SAIRs) are powerful sensors for high-resolution observation of the Earth at low microwave frequencies. However, the future application of this new technology is limited by its large number of element antennas and receiving channels. In order to further reduce the complexity of the whole system, the circular and rotating array configurations have been proposed. Their disadvantages lie primarily in more complicated image reconstruction, because the conventional fast Fourier transform (FFT) method could not be used directly for image reconstruction. This letter introduces an iterative method for the reconstruction of radiometric brightness temperature maps from nonuniform visibility function samples. This method combines the conjugate gradient algorithm with min-max nonuniform FFT approach, which has been shown to provide considerably improved image quality relative to the traditional gridding method. Simulations for SAIRs were performed and showed better image quality in comparison to the traditional frequency interpolation method.