Concurrent imaging of chlorophyll fluorescence,Chlorophyll a content and green fluorescent proteins‐like proteins of symbiotic cnidarians

Research on photosynthetic cnidarians has been mainly focused on the symbiosis established between the cnidarian host and its dinoflagellates endosymbionts from genus Symbiodinium. Despite the potential of imaging techniques for assessing the spatial distribution of key parameters of cnidarian photobiology, such as photochemical activity, chlorophyll a content or green fluorescent proteins (GFPs), to our best knowledge, no study has ever attempted to simultaneous map these three features. In this study, we developed a modified imaging pulse amplitude fluorometer by applying excitation light of different wavelengths and selectively detecting short spectral bands through bandpass filters. The imaging system was used to sequentially excite and quantify chlorophyll variable fluorescence (maximum quantum yield of photosystem II, F_v/F_m), Chl a content (normalized difference vegetation index) and relative content of GFPs. The spatial distribution of these photophysiological parameters was mapped both horizontally, across the surface of the soft corals Sarcophyton cf. glaucum and Sinularia flexibilis and the zoanthid Protopalythoa sp., and vertically, throughout a vertical section of S. cf. glaucum. Results showed bleached areas within each individual coral colony and registered photophysiological changes with S. cf. glaucum tissue depth. Analysis of Protopalythoa sp. polyps’ expansion revealed differential surface patterns of NDVI and GFP concentration, and a negative relation between these latter parameters within each polyp. This novel non‐invasive approach allowed a high‐resolution characterization of the spatial relationship between these key parameters through the analysis of image information on a pixel‐by‐pixel basis, which has great potential for investigating the physiological state of symbiotic associations.     

粤西沿海一次赤潮事件的三维观测

From November 24 to 26, 2014, a red tide event occurred in the offshore water off the Hailing Island located at the western Guangdong coast. The red tide appeared as pink strips distributed within 3 km in the offshore water and extended for about 10 km along the shoreline. During the flood tide, the pink seawater rushed to the beach with breaking waves, forming foam strips on the beach. Guangdong Province Key Laboratory for Coastal Ocean Variation and Disaster Prediction Technologies, Guangdong Ocean University, emergently responded to the event and organized three-dimensional observations from the air, onboard and on beach. The preliminary analyses of the cruise data and water samples indicate that the event was induced by nontoxic Noctiluca scintillans, of which the concentration reaches as high as 4 200 cells/L near the surface and 2 600 cells/L at the bottom.     

应用实时定量PCR技术检测秦皇岛近岸海域褐潮原因种——抑食金球藻(Aureococcus anophagefferens)

Aureococcus anophagefferens, a small pelagophyte algae, has caused brown tide blooms in coastal waters of Qinhuangdao in recent years, presenting significant negative impacts on the shellfish mariculture industry. Under standard light microscopy, it is visually indistinguishable from other small algae in field samples due to its extremely small size. In this study, quantitative polymerase chain reaction(q PCR) based on 18 S r DNA sequences was developed and used to detect and enumerate A. anophagefferens. A linear regression(R2 = 0.91) was generated based on cycle thresholds value(Ct) versus known concentrations of A. anophagefferens. Twenty-two field samples collected in coastal waters of Qinhuangdao were subjected to DNA extraction and then analyzed using q PCR. Results showed that A. anophagefferens had a wide distribution in coastal waters along Qinhuangdao. Elevated A. anophagefferens abundance, category 3 brown tide blooms(200 000 cells/m L) occurred at Dongshan Beach and Tiger-stone Beach in August in 2013. In shellfish mariculture areas along coastal waters of Qinhuangdao, 4 stations had category 3 blooms, and 6 stations had category 2 blooms(35 000–200 000 cells/m L) in August and all stations had category 1 blooms(0 to ≤35 000 cells/m L) in October. Quantitative PCR allows for detection of A. anophagefferens cells at low levels in filed samples, which is essential to effective management and prediction of brown tide blooms.     

3种海洋微藻微胶囊培养的初步研究

用12mg/mL羧甲基纤维素钠(CMC)、10mg/mL海藻酸钠(SA)和0.5mmol/mL CaCl2制备亚心形扁藻、绿色杜氏藻和球等鞭金藻8701的中空海藻酸钙微胶囊,3种微藻在微胶囊内生长表现突出,最高细胞密度亚心形扁藻为70.4×106/mL,绿色杜氏藻为100.9×106/mL,球等鞭金藻8701为264.1×106/mL,分别是悬浮培养微藻最高密度的35倍、27倍和84倍,结果说明微胶囊化培养是微藻高密度培养的有效方式。     

日照水库及周边区域综合地质条件分析

日照水库是1959年建成的一座大(二)型水库,位于日照市东港区驻地以西16 km处的付疃河中游,控制流域面积548 km2,是日照城市及国民经济发展的最重要水源保障。近年来日照市经济社会飞速发展,特别随着"山东精品钢基地"等大项目的开工建设,供水安全形势愈发严峻,如何维护日照水库和保护这一水源地更是被提到了重要的议事日程。先行研究分析流域地质条件,是做好日照水库保护建设工作的重要基础。     

深度学习支持下的丘陵山区耕地高分辨率遥感信息分区分层提取方法

耕地是丘陵山区稀缺的土地资源,具有地形条件复杂、种植结构多样的特点,导致了山地耕地信息难以快速、准确获取,并且基于传统的遥感数据及遥感监测方法开展山区耕地信息快速自动提取比较困难。针对这一问题,本文以西南山区贵州省息烽县作为试验区,根据地理空间异质性特征,提出分区控制、分层提取的耕地形态信息提取思路,构建了一种地貌单元约束条件下的分区分层耕地形态信息的提取方法。该方法首先根据地貌-植被特征将试验区划分为平坝区、山坡区、林草区3类地理分区;然后在每类分区基础上,根据耕地所呈现的视觉特征划分为不同的类型,对不同类型的耕地分别设计不同的深度学习模型进行分层提取。试验结果证明,该方法对山区复杂地形背景噪声具有较好的抑制作用,所提取的耕地地块信息相比于传统方法更符合实际耕地的实际分布形态,有效地减少了漏提率和错提率。     

北斗二号与北斗三号完好性参数设计差异分析

针对能够标识北斗二号与北斗三号系统服务完好性的参数差异问题,该文从完好性参数定义、导航电文结构、参数内容和播发方式等方面对北斗二号和北斗三号系统完好性参数播发现状进行了比对分析,并对各类参数的工程实现情况进行了比较,结果表明:在兼容北斗二号基本导航服务相关系统完好性参数的基础上,北斗三号增加了可快速标识服务精度及预测精度的完好性参数;受多方面的约束与局限,北斗二号仅具备提供单频改正的广域差分增强服务的能力,且无相关国际标准规范支撑,而北斗三号为能获取国际民航组织测试认证,已立足于国际相关标准规范进行了可支撑单、双频改正的星基增强服务工程实现.     

航空重力测量的空中数据处理与分析

针对航空重力测量的原始扰动数据,本文介绍了数据处理的一般流程,内容包括原始数据检查、零点漂移校正、平均高程面归算、交叉点搜寻、内符合精度评价、离散数据的格网化等方面,详细说明了每一步数据处理的理论模型,并通过工程实例对计算结果进行了讨论和分析,为今后开展大规模航空重力测量业务作出一定的尝试和探索.     

河北矾山超钾质岩浆氧逸度及含水性特征的磷灰石地球化学记录

产于华北克拉通北缘的晚三叠世矾山超钾质碱性岩杂岩体主要由单斜辉石岩和辉石正长岩等组成,以发育内生磷灰石-磁铁矿矿床而闻名。本文选取矾山杂岩体中的磷灰石为研究对象,通过测试磷灰石的主微量元素及原位Sr-Nd同位素组成以探讨母岩浆的氧逸度及含水性特征。研究表明,矾山磷灰石以轻稀土元素富集、重稀土元素亏损为特征,显示出缺乏显著Ce异常的“右倾”的稀土配分型式,且具有较低的Ga含量及较高的δEu值,暗示杂岩体具有较高的岩浆氧逸度,这也得到了磷灰石Mn氧逸度计的支持。所有磷灰石以高Sr、低Y为特征,具有较高的Sr/Y(>30)和La/Yb(>90)比值,且Sr/Y与La/Yb存在明显的正相关关系,表明矾山超钾质岩浆具有较高的含水量。矾山磷灰石的原位Sr-Nd同位素投点位于地幔序列,明显偏离地壳趋势。母岩浆的“氧化且富水”特征是矾山杂岩体得以发生磷灰石-磁铁矿矿化的重要因素,其主要与板片俯冲过程中蚀变洋壳对岩石圈地幔的交代作用有关。通过对比矾山杂岩体与全球范围内其他地质环境中磷灰石的成分数据,发现具有“高Sr、低F、少Cl”特征的磷灰石可作为识别超钾质碱性岩杂岩体(及其相关金属矿床)的...     

Similarity model of feed support system for FAST

A new design of feed support system for Five hundred meter Aperture Spherical Telescope (FAST) is proposed in this paper. According to the similarity theory, a 1:15 scale model of feed support system has been built to make systemic research on the feasibility of the system. Then the control system and hardware structure of the feed support system are illustrated. A complete astronomical observation track is run by the scale model and the experiments results demonstrate that the new feed support system can satisfy the observation accuracy requirement of FAST.