中华哲水蚤(Calanus sinicus)个体核酸含量及其对生长状况的指示作用

采用荧光染色法对黄、东海春、秋季中华哲水蚤(Calanus sinicus)个体核酸含量进行了研究,初步探讨了RNA∶DNA比对中华哲水蚤生长状况的指示作用。结果表明,中华哲水蚤在饥饿培养24h后RNA∶DNA比值显著降低;雄性个体RNA含量和RNA∶DNA比值显著低于雌体和C5期幼体;春、秋季(2009年5月和2010年11月)个体RNA含量和RNA∶DNA比值存在显著的空间变化,两者最高值均出现在近岸;两个月份DNA含量相对稳定,在2.5—3.5μg/ind之间;个体RNA含量和RNA:DNA比值与叶绿素之间显著正相关,说明饵料是中华哲水蚤的生长过程中重要的影响因素。     

软悬挂状态下隔水管动力特性分析

建立了软悬挂状态下隔水管动力分析模型,采用有限单元法对模型进行离散,结合Newmark-β法对动态方程求解,并对模型进行验证,最后选取南海某口深水井实际参数,对软悬挂状态下隔水管动力特性进行分析。研究结果表明:软悬挂状态下隔水管底端横向位移最大,顶部铰接处弯矩最大。软悬挂状态下,隔水管横向位移与弯矩随着海底隔水管总成(LMRP)重量增加而减小,随着平台运动速度、平台航向与海流方向夹角增大而增大;回收隔水管减小悬挂长度有利于减小最大横向位移,但会造成弯矩轻微增加;采用旋转刚度适当较大的挠性接头则有利于减小隔水管挠性接头以下整体位移与弯矩。研究成果为钻井平台避台撤离时隔水管的安全提供理论指导。     

Seasonal variability of community structure and breeding activity in marine phytal harpacticoid copepods on Ulva pertusa from Pohang,east coast of Korea

Seasonal changes in community structure and reproductive status of phytal harpacticoid copepods in the shallow sublittoral bottom at two sites (Masan-ri and Guryongpo) in Pohang (Korea) are described monthly over a period of 1 year (October 1996 to September 1997). A total of 36 harpacticoid species was identified and the numerically dominant copepods were made up of the families Porcellidiidae and Tisbidae. Although the number of species did not show a seasonal trend, total harpacticoid density revealed a favorable distribution for the warmer season (spring and summer) at both sites. Multiple linear regression analyses showed that univariate indices such as density, evenness and diversity are closely associated with certain environmental parameters. For example, the dominant species fluctuated seasonally in abundance and their maximum densities were found to be temperature- (+ with Porcellidium ofunatense) and nutrient-dependent (+ with Scutellidium longicauda acheloides, + with Zaus unisetosus, and ? with P. wandoensis). In addition, the dominant species appeared to breed year round and their reproductive indices are significantly correlated with some environmental parameters such as temperature (?), pH (+), and phosphate (+). Three species (S. l. acheloides, P. wandoensis, and P. ofunatense) showed maximum density two or three months after their reproductive activity reached a maximum. Overall, the seasonal changes in a phytal harpacticoid community could be explained by combinations of environmental parameters supporting the complexity and biodiversity for this specific group of species in coastal ecosystems.     

线性偏振光激发的错位表面等离子体激元纳米结构聚焦

利用扫描近场光学显微镜观测并分析了两种表面等离子体激元纳米结构对表面等离子体激元(SPP)的激发和聚焦现象. 用线偏振光照射有半个周期相位差的环状沟槽结构与有半个周期位相差的环状狭缝结构,得到了单点的SPP聚焦. 有限时域差分法的模拟结果验证了实验中观测的现象. 这两种相位错位的表面等离子体激元纳米结构,突破了由于干涉导致的线偏振光不能得到单个聚焦点的限制. 与采用径向偏振光激发而得到单个聚焦点的方法相比,线偏振光不需要聚焦,也不需要将光束中心对准纳米结构的几何中心即可得到单点聚焦.     

Genetic variation of the small yellow croaker (Larimichthys polyactis) inferred from mitochondrial DNA provides novel insight into the fluctuation of resources

The small yellow croaker (Larimichthys polyactis) belongs to the family Sciaenidae, which is an offshore warm fish species and widely distributed in the western Pacific. In this study, the variation of genetic diversity and genetic differentiation among L. polyactis populations was analyzed by mitochondrial DNA control region. A total of 110 polymorphic sites were checked, which defined 134 haplotypes. High level of haplotype diversity (h=0.993±0.002) was detected in the examined range. Population genetic structure analyse (analysis of molecular variance, F_st) showed there were high gene flow among L. polyactis populations. The result showed that there were relatively high genetic diversity and low genetic differentiation among the Yellow Sea and the East China Sea populations, which can be attributed to diverse habitats, wide distribution range and high mutation rate of control region. Using phylogenetic methods, coalescent analyses (neutrality tests, mismatch distribution analysis, Bayesian skyline analyses) and molecular dating interpreted in conjunction with paleoclimatic and physiographic evidence, we inferred that the genetic make-up of extant populations of L. polyactis was shaped by Pleistocene environmental impacts on the historical demography of this species. Besides, relatively constant genetic diversity and larger effective population size were detected in recent L. polyactis population. The result showed that the fishing policy certainly, such as the summer closed fishing, played a role in protecting resources of L. polyactis. This study can offer a wealth of biological novelties which indicates genetic structure of L. polyactis population and provides the foundation for resources protection and policy setting.     

CMIP5模式对中国近海海表温度的模拟及预估

基于观测和再分析资料;利用多种指标和方法评估了国际耦合模式比较计划（CMIP5）中21个模式对中国近海海温的月、季节和年际变化模拟能力。多模式集合能够再现气候平均意义下近海海温的空间分布特征;但量值上存在一定的低估。在渤海和黄海;集合平均与观测差别比较明显。在年际尺度上;与观测数据对比;模式模拟海温与Niño3指数相关性较小。中国近海海表面温度在1960-2002年有明显的升高趋势;从2003年开始增温趋缓。评估结果表明;ACCESS1.0、BCC-CSM1.1、HadGEM2-ES、IPSL-CM5A-MR、CMCC-CM、FGOALS-g2、CNRM-CM5-2、INMCM4八个模式对中国近海海温的变化有较好的模拟能力。利用ACCESS1.0、INMCM4、BCC-CSM1.1、IPSL-CM5A-MR、CMCC-CM这5个模式结果对中国近海海温未来的变化进行了预估。在RCP4.5、RCP8.5情景下;未来近100年中国近海海温有明显升高趋势;最优模式多模式集合平均增温分别可达到1.5℃、3.3℃;净热通量变化和平流变化共同促进了东海升温。     

Oceanic response to Typhoon Nari (2007) in the East China Sea

The oceanic response to a typhoon in the East China Sea (ECS) was examined using thermal and current structures obtained from ocean surface drifters and a bottom-moored current profiler installed on the right side of the typhoon’s track. Typhoon Nari (2007) had strong winds as it passed the central region of the ECS. The thermal structure in the ECS responded to Typhoon Nari (2007) very quickly: the seasonal thermocline abruptly collapsed and the sea surface temperature dropped immediately by about 4°C after the typhoon passed. The strong vertical mixing and surface cooling caused by the typhoon resulted in a change in the thermal structure. Strong near-inertial oscillation occurred immediately after the typhoon passed and lasted for at least 4–5 days, during which a strong vertical current existed in the lower layer. Characteristics of the near-inertial internal oscillation were observed in the middle layer. The clockwise component of the inertial frequency was enhanced in the surface layer and at 63 m depth after the typhoon passed, with these layers almost perfectly out of phase. The vertical shear current was intensified by the interaction of the wind-driven current in the upper layer and the background semi-diurnal tidal current during the arrival of the typhoon, and also by the near-inertial internal oscillation after the typhoon passage. The strong near-inertial internal oscillation persisted without significant interfacial structure after the mixing of the thermocline, which could enhance the vertical mixing over several days.     

温度和饵料对双刺纺锤水蚤产卵率和孵化率的影响

Acartia bifilosa (Copepoda: Calanoida) is a common species in offing of global waters and it often becomes dominant species in some estuaries. This species develops different strategy according to variation of environment. Despite its ecological role, Acartia bifilosa also has been concerned as live feeds in aquaculture, but study on egg production rate of this species in saturated diet in different temperature and different diet is so far not available. In order to interpret the importance of temperature and diet on natural population variation and also on aquaculture as foods of larval fish, the functional response of reproductive success of Acartia bifilosa was quantified in the laboratory using different temperatures and diets. Acartia bifilosa was captured in Jiaozhou Bay and acclimated to corresponding temperature for 3~4 days. In order to reduce the effect of large extent temperature range on organism, we captured Acartia bifilosa from March to June and acclimated them to temperature which is adjacent to natural temperature. Daily egg production rate (EPR, eggs female-1day-1) was detected for 11~15 days at 5 different temperatures ranged from 8.0 to 23 °C and all the females was feed on saturated diet all through the experiments. EPR on first day was not calculated in the mean EPR to eliminate the effect of different diets. This result showed that Acartia bifilosa spawned continuously during the experiment days and no obvious regulation was found. EPR was positively correlated with temperature from 8.0 to 23 °C. The highest mean EPR was observed at 23 °C (7.3 eggs female-1day-1), and the lowest value was found at 8 °C (3.8 eggs female-1day-1). The effect of diets on EPR was evaluated at two different temperatures (8.0 °C and 12.5 °C). Same trends were found at the two temperatures: mean EPR fed on Chaetoceros sp. was higher than that fed on Skeletonema costatum, and during the 14-day experiments, EPR was higher for females fed on Skeletonema costatum than chaetoceros sp. in the first few days (3~5 days), but it changed reversely in the subsequent days until the end of the experiments. Hatched eggs were observed in the two experiments at temperature 8°C and 12.5 °C and no diapause eggs were found. The hatching success rate was low at 8°C but reached 93.2% at 12.5 °C. According to the results in this paper, we detected the comfortable temperature range for EPR, and evaluated the effect of Skeletonema costatum which often brings out red tide in natural waters. These results are beneficial to ecological research for explaining population variation and population recruitment of this species, These data also can be used in Acartia bifilosa aquaculture.     

大洋多金属结核矿区沉积物土工性质

利用1994年大洋多金属结核调查所获得的土工资料,结合以往有关资料进行了综合分析。研究了大洋沉积物类型、沉积物土工性质、土质强度等,为未来海底工程设施和多金属结核开采设备的设计与制造提供依据。     

深圳大鹏湾大鹏半岛沿岸海域的一次调查与分析

一九九八年三月,我们在大鹏半岛海域调查时,恰逢深圳大鹏半岛近岸海域赤潮发生。结合我们对该区域内沿海水域调查结果进行分析,给出该海域的潮汐、潮流的基本特征及当时该海域的水质状况,为规划、保护该海域的海洋环境提供科学依据,并为研究赤潮发生成因提供参考资料。