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二色裂江珧EST和SOD同工酶组织特异性研究

取二色裂江珧 (Pinna bicolor)的消化盲囊、肾脏、后闭壳肌肌肉、外套膜和鳃等 5种组织 ,用垂直板聚丙烯酰胺凝胶电泳对酯酶 (EST)和过氧化物歧化酶 (SOD)同工酶在上述组织中的表达和分布进行了比较研究 ,并对酶谱表型及位点表达进行了分析。结果表明 ,二色裂江珧组织内的 EST和 SOD存在不同程度的组织特异性 ,EST共检测出 15条酶带 ,SOD检测出 8条酶带 ,且两种同工酶在消化盲囊中活性最高。推测 SOD由 2个座位编码 ,可分为 s- SOD二聚体和 m- SOD四聚体     

Measuring Tourist’s Water Footprint in a Mountain Destination of Northwest Yunnan, China

Monitoring and minimizing tourist’s water consumption is essential for the sustainable development of mountain destinations in the world. However, available data and a generally accepted protocol on such measurement are still limited. This study uses water footprint accounting to quantify tourist’s water demand in the Liming valley, a World Heritage site and a rapidly growing tourism destination in Northwest Yunnan, China. Both the water for direct and indirect use is taken into account based on the consump...     

Degradation of wetlands on the Qinghai-Tibet Plateau: A comparison of the effectiveness of three indicators

The Qinghai-Tibet Plateau encompasses a large quantity of wetlands, some of which have been degraded to varying severity levels. In the literature, a number of degradation indicators have been proposed to evaluate ecological health of wetlands, but their effectiveness in the plateau environment remains unknown. In this study, we assessed the effectiveness of three degradation indicators, soil moisture content at 10 cm deep, vegetative cover, and density of pika burrows. The degradation severity of wetlands in Maduo County on the Qinghai-Tibet Plateau is enumerated at four levels, intact, slight, moderate and severe. Analysis of 106 samples collected in the field demonstrates that the density of pika burrows is the least reliable indicator. By comparison, vegetative cover and underlying soil moisture content are more reliable, even though neither is a perfect indicator as the difference among adjacent levels of severity as revealed by t-test is not always statistically significant. The imperfection of vegetative cover as an indicator is due to its variation among different types of wetlands. The limitation of moisture content is attributed to its non-linear relationship with wetland degradation. Above the threshold of about 50% in moisture content wetlands are unlikely to be degraded. It is recommended that moisture be measured at the point near the surface and vegetative cover be further differentiated by species in order to improve their effectiveness.     

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西安市地面沉降探讨

本文在讨论西安市地面沉降特征的基础上,论述了西安市地下水开采量、承压水位变化与地面沉降三者之间在时间和空间上的对应关系,初步认定大量开采地下水是地面沉降主要原因。典型水准点沉降量与邻近自备井承压水位变化相关分析表明,两者间相关性极强。经综合分析市区地貌、第四系地质、工程地质和水文地质特征并进行工程地质分层后,可确定出市区地面沉降的第一和第二主要压缩层。据地面沉降与承压水位下降值回归方程所预测的地面沉降发展趋势表明,西安地面沉降今后还会继续发展,将对西安城市建设产生严重影响,故应采取相应治理措施。     

取土器几何尺寸对饱和软黏土样结构的扰动研究

基于颗粒流软件模拟,以吹填淤泥为例,分析了取土器的内径、切角、壁厚等尺寸参数对高黏性软土微观动力学特性的影响;通过分层方法,比较了7组土样层间颗粒特征拐点的运动行为。模拟结果表明:土样的颗粒在底部形成对流位移场,对流处上方颗粒主要受拉膨胀,对流处下方颗粒主要受压收缩,由此造成孔隙率的规律变化和层理的弯曲变形。7组参数对应土样的孔隙率曲线与初始孔隙率曲线的交点同颗粒位移场的对流位置存在协同关系。土样层理表现为向下弯曲变形,层间颗粒位移行为存在特征拐点,拐点之间颗粒扰动行为不明显;反之,颗粒扰动行为较大,这种变形特征可拟合为下开口的旋转抛物面,且曲面方程的曲面形状拟合系数a值能初步评估7组土样的扰动程度。层间特征拐点的连线可再拟合为旋转抛物面,抛物面体积与土样体积的比值可用来评估土样的原状性。     

青州市表层土壤元素地球化学组合特征研究

聚类分析和因子分析可以获得土壤元素地球化学组合特征及其差异性。对青州市表层土壤样品数据进行分析研究,通过聚类分析,绘制表层土壤元素聚类谱系图,将23种元素或指标分为5个元素组合簇群及2个单元素簇,研究各元素间的组合特征,探讨其相关性、聚集性及其指示意义;通过因子分析,找出有代表性的因子,用其代表变量,绘制典型因子得分等值线图,并从中分析不同元素组合的区域分布基于何种因素,用11个代表性因子的分布特征就基本可以代表青州市表层土壤23项原始变量的分布特征,并对F1,F2,F3主因子进行了地质解释。聚类分析与因子分析相结合,利于表层土壤中元素的共生组合特征及其差异性研究,利于对研究区表层土壤异常进行归纳总结。     

迟缓爱德华氏菌致病相关因子的研究进展

迟缓爱德华氏菌(Edwardsiealla tarda)是引起多种鱼类产生爱德华氏菌病(edwardsiellosis)的病原菌。随着水产养殖业的日益发展,致病性迟缓爱德华氏菌对水产养殖业的危害也越来越严重。有关迟缓爱德华氏菌的致病机理至今还没有系统、清晰的阐释,但近些年对其致病相关因子的研究已逐渐深入。文中对迟缓爱德华氏菌的生物分型、快速检测技术等进行了简要阐述,并对该病原菌的主要致病相关因子,包括黏附因子、抵抗宿主免疫机制的酶类、各种毒素、Ⅲ型和Ⅵ型分泌系统、载铁体及磷酸盐特异转运操纵子进行了详细阐述,以期为迟缓爱德华氏菌致病机理的进一步深入研究及其防治提供参考和借鉴。