Establishment and application of an intelligent treating method for oil spill identification

In the identifying process of an oil spill accident, manual integral and artificial visual comparison are commonly used at present to determine the oil spill sources, these methods are time-consuming and easily affected by human factors. Therefore, it is difficult to achieve the purpose of rapid identification of an oil spill accident. In this paper, an intelligent method of automatic recognition, integration and calculation of diagnostic ratio of Gas Chromatography-Mass Spectrometer (GC/MS) spectrum are established. Firstly, four hundreds of samples collected around the world were analyzed using a standard method and Retention time locking technology (RTL) was applied to reduce the change of retention time of GC/MS spectrum. Secondly, the automatic identification, integration of n-alkanes, biomarker compounds, polycyclic aromatic hydrocarbons and calculation of the diagnostic ratios were realized by MATLAB software. Finally, a database of oil fingerprints were established and applied successfully in a spill oil accident. Based on the new method and database, we could acquire the diagnostic ratios of an oil sample and find out the suspected oil within a few minutes. This method and database can improve the efficiency in spilled oil identification.     

Enhanced a novel β-agarase production in recombinant Escherichia coli BL21(DE3) through induction mode optimization and glycerol feeding strategy

Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food,cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode optimization for high cell density and β-agarase production was established, which could effectively control acetate yield. First, exponential feeding strategy of glycerol with different overall specific growth rates(μ) was applied in the pre-induction phase. The results showed that the low μ(μ=0.2) was suggested to be the optimal for cell growth and β-agarase production. Second, the effects of induction temperature and the inducer concentration on cell growth and β-agarase production were investigated in the post-induction phase. When induced by isopropyl-β-d-thiogalactoside(IPTG), the strategy of 0.8 mmol/L IPTG induction at 20℃ was found to be optimal for β-agarase production. When cultivation was induced by continuous lactose feeding strategy of 1.0 g/(L·h), the β-agarase activity reached 112.5 U/mL, which represented the highest β-agarase production to date.Furthermore, the β-agarase was capable of degrading G. lemaneiformis powder directly to produce neoagarooligosaccharide, and the hydrolysates were neoagarotetraose(NA4) and neoagarohexaose(NA6). The overall research may be useful for the industrial production and application of β-agarase.     

多极化SAR溢油探测影响因素分析研究

Polarimetric synthetic aperture radar(SAR) oil spill detection parameters conformity coefficient(μ), Muller matrix parameters(|C|, B_0), the eigenvalues of simplified coherency matrix(λ_(nos)) and the influence of SAR observing parameters, ocean environment and noise level are investigated. Radarsat-2 data are used to make systematic analysis of polarimetric parameters for different incidences, wind speeds, noise levels and the ocean phenomena(oil slick and look likes). The influence of the SAR observing parameters, the ocean environment and the noise level on the typical polarimetric SAR parameter conformity coefficient has been analyzed. The results indicate that conformity coefficient cannot be simply used for oil spill detection, which represents the image signal to the noise level to some extent. When the signals are below the noise level for the oil slick and the look likes, the conformity coefficients are negative; while the signals above the noise level corresponds to positive conformity coefficients. For dark patches(low wind and biogenic slick) with the signal below the noise,polarization features such as conformity coefficient cannot separate them with oil slick. For the signal above the noise, the oil slick, the look likes(low wind and biogenic slick) and clean sea all have positive conformity coefficients, among which, the oil slick has the smallest conformity coefficient, the look likes the second, and the clean sea the largest value. For polarimetric SAR data oil spill detection, the noise plays a significant role. So the polarimetric SAR data oil spill detection should be carried out on the basis of noise consideration.     

热带西太平洋砂壳纤毛虫的多样性

为了探明热带西太平洋海区砂壳纤毛虫的多样性、垂直分布及纬向变化,我们于2012年11-12月在该海区四个断面采集了0-200 m的水样。共检出砂壳纤毛虫39属124种,多数种类喜好生活在表层和次表层。砂壳纤毛虫种丰富度、丰度以及多样性指数的高值主要出现在比叶绿素浓度最大值稍浅的位置,与温度和叶绿素浓度显著正相关,与盐度和采样深度显著负相关。多数优势种与环境因子间的相关性不显著。热带西太平洋海区砂壳纤毛虫多样性极高,各站种丰富度在25-52之间。在从表层至75 m处的多数采样点香农指数均大于3。该海区砂壳纤毛虫冗余种的比例很高,占砂壳纤毛虫总种类数的87.90%,贡献了砂壳纤毛虫60.38%的丰度,表明热带西太平洋海区砂壳纤毛虫群落对饵料组成和摄食压力改变的应对能力很强,稳定性很高。     

太平洋深海沉积物来源的菌株Tenacibaculum sp.HMG1对孔雀石绿降解特性的研究

本文在太平洋深海沉积物中分离得到一株孔雀石绿降解菌株,鉴定命名为Tenacibaculum sp.HMG1。通过菌株生长实验和高效液相色谱的研究表明, HMG1菌株可以在20 mg/L的孔雀石绿中维持较快的生长速率,并且在12 h内可降解98.8%的孔雀石绿,这证明该菌株具有很高的孔雀石绿耐受能力和降解活性。通过基因组测序在HMG1菌株发现一条过氧化物酶基因可能参与了孔雀石绿的降解,随后利用原核表达获得了相应的重组蛋白。实验表明,该重组过氧化物酶具有极强的活性,可在1000 mg/L的孔雀石绿中发挥降解功能。本文利用液相色谱-质谱联用（LC-MS）技术对孔雀石绿的菌株降解产物和重组酶降解产物进行鉴定,并基于鉴定结果推测了两种降解途径。结果发现两种降解方式存在共同的降解途径。此外,孔雀石绿降解条件的实验结果证明重组过氧化物酶可以在低温（20℃）、复杂的pH值（6.0–9.0）、高盐度（100 mmol/L）、金属离子和EDTA等反应条件下依旧维持很高的孔雀石绿降解活性。以上实验结果表明,HMG1菌株和重组过氧化物酶均在孔雀石绿污染生物修复方面具有很大潜力。     

基于GNSS的LEO卫星TRAIM可用性算法及分析

详细研究了基于GNSS的LEO卫星接收机授时自主完好性（TRAIM）故障检测数学模型以及可用性算法。以GPS和BDS组合为例,分别对500 km、650 km、800 km和1 400 km等几种典型轨道高度的卫星,开展基于GNSS的LEO卫星TRAIM可用性仿真分析。结果表明,在告警阈值100 ns时,基于GPS+BDS组合系统的TRAIM检测可用概率达到99.1%以上,基本满足LEO卫星TRIAM检测可用性需求。     

InSAR滑坡探测技术研究——以金沙江乌东德水电站段为例

采用3类InSAR产品和DEM数据开展金沙江流域乌东德水电站段的潜在滑坡探测,成功识别出多处已知和未知的滑坡点,并探测出滑坡体的形态及稳定性,提供了一种高效的大范围滑坡探测技术。同时采用小基线集InSAR技术对金坪子滑坡进行监测,不仅获得该滑坡的空间分区特征,也获取重点滑坡区的时间序列结果,并且与地面监测结果比较,精度达1.8 cm。展示了不同InSAR技术在不同尺度滑坡调查与监测中的应用特点。     

基于灯光数据的中国县域城镇建设用地产出效率时空演变特征

本文以灯光密度表征建设用地产出效率,采取KDE、ESDA、SDE等方法对中国县域尺度2000-2015年建设用地产出效率的总体特征、时空演变以及方向分布进行分析,研究发现：① 夜间灯光总亮度与建设用地出产出存在较强的相关关系,说明可用灯光总亮度表征建设用地产出,以灯光密度反映建设用地产出效率具有一定的科学性。② 建设用地产出效率东高西低,持续上升;增长速度西高东低,中部居中,但区域间年均增长率最大相差0.56%,整体较为均衡。③ 虽然建设用地产出效率由2000年的0.008单位灯光/km²持续上升至2015年的0.025单位灯光/km²,但其核密度曲线始终处于中低水平且右侧区间不断扩大,说明中国建设用地产出效率总体水平较低,呈现出中低水平俱乐部收敛的特征。④ 建设用地产出效率的全局 Moran's I指数都大于0,建设用地产出效率存在正向空间分布特征,且其局部空间格局变化较小,存在稳中有动,强中有弱,弱中有强的特点。⑤ 方向分布上,建设用地产出效率的方位角始终在72.420^o与81.066^o之间,说明东北-西南方向为建设用地产出效率的主要方向,且标准差椭圆的主轴与辅轴标准差都有所增加,说明在建设用地产出效率的主要方向与次要方向上都发生了分散现象。     

北京城市绿化的时空动态分析

城市绿化覆盖是城市生态系统的重要组成部分,合理的绿化率和绿化布局可以改善城市环境,提高城市人居适宜性。研究中将2005、2010、2015年北京市土地利用（LUC）数据中的城市居民用地作为城区范围,应用Landsat 5、GF-1影像数据和MODIS产品,利用支持向量机的监督分类方法,提取了2005、2010、2015年的北京市城市绿化覆盖数据,并获取了同期的植被指数（NDVI）数据;继而以城市绿化覆盖率、绿化覆盖均匀度和植被指数为评价指标,在公里栅格和行政区2个尺度上探讨了北京市城市绿化覆盖的空间分布格局和时间变化动态特征。研究表明：① 3个指标在空间和时间2个维度、区县和栅格2个尺度上都表现一致。这反映北京市过去10年中,在绿化面积增加的同时,绿化的空间布局得到优化改善,绿化的质量得到提高。② 2005-2015年,北京市城市绿化覆盖面积由518.93 km² 增加到1405.54 km²,绿化覆盖率由39.9%增加到49.13%,绿化覆盖均匀度由0.598增加到0.653,植被指数由0.42增加至0.5。③ 北京市城市绿化建设存在明显的时空差异。中心城区绿化建设缓慢,成效不明显;重大绿化建设成果主要集中在城市边缘地区和远郊区县。城市绿化改善过程主要发生在2005-2010年。     

顾及多分析尺度的地形部位面向对象分类方法

地形部位是地表形态的基本单元,其分类和提取在地貌发育、数字土壤制图、景观生态制图等领域有着重要的应用。康鑫等提出的多尺度Geomorphons地形部位分类法（简称多尺度Geomorphons法）利用高程相对差异信息和地形部位多尺度特征,可避免受地形属性计算及单一分析尺度约束而误分类,然而其存在分类破碎及分析尺度域难以确定的问题。基于此,本文以多尺度Geomorphons法为基础,提出了其适宜分析尺度域确定方法,建立了以初始地形部位数据层组合的对象多尺度分割和分类方法,进而构建了顾及多分析尺度的地形部位面向对象分类方法。以陕北黄土高原区域5 m分辨率DEM为实验数据,对面向对象分类方法进行了验证与评价。实验结果表明：①均值变点法可有效解决分析尺度域难以确定的问题,实验样区适宜分析尺度域为[5×5, 33×33]栅格单元;②以0,255为二值化的地形部位数据层组合适用于多尺度分割,尺度、形状及紧致度参数组合影响分割结果,且对于实验样区存在最优分割参数;③与多尺度Geomorphons法相比,本文方法得到的地形部位分类结果完整性较好,在地表形态对应和地理认知等方面更具合理性。