Isolation of Protoplasts from Undaria pinnatifida by Alginate Lyase Digestion

1　Introduction　Protoplastsserveasabasicandversatiletoolforge neticengineeringandbiochemicalresearchforseveralreasons :theymayregeneratetheirwallsandprovideamodelsystemforstudyingwallbiogenesis ,theirlysisprovidesagentlemeansofpreparingsubcellularor ganelles ,andthemembraneexpositionallowsgeneticmanipulationsinvolvingfusionoruptakeofnucleicacidstobepossible .Theseadvantagesarelessachiev ablewhenusingintactcells (Liciaetal.,1999) .Incomparisonwithlandplantsandunicellularalgae ,on lylimitedrepo…     

Identification of paralytic shellfish toxin-producing microalgae using machine learning and deep learning methods

Journal of Oceanology and Limnology - Paralytic shellfish poisoning (PSP) microalgae, as one of the harmful algal blooms, causes great damage to the offshore fishery, marine culture, and marine...     

CLIMATIC TREND INDICATED BY VARIATIONS OF GLACIERS AND LAKES IN THE TIANSHAN MOUNTAINS

ＣＬＩＭＡＴＩＣＴＲＥＮＤＩＮＤＩＣＡＴＥＤＢＹＶＡＲＩＡＴＩＯＮＳＯＦＧＬＡＣＩＥＲＳＡＮＤＬＡＫＥＳＩＮＴＨＥＴＩＡＮＳＨＡＮＭＯＵＮＴＡＩＮＳ￥ＨｕＲｕｊｉ；ＹａｎｇＣｈｕａｎｄｅ；ＭａＨｏｎｇ；ＪｉａｎｇＦｅｎｇｑｉｎｇ（ＸｉｎｊｉａｎｇＩｎ...     

对南极地区气温的聚类分析

对南极地区的多年月平均气温资料进行聚类分析,结果表明,可以分为极区内陆、海湾区域、东南极沿岸和南极半岛地区四大类型。南极半岛地区一些位于南极圈之外的考察站反映的仍是南极气候状况。     

上海中心城区河流水系百年变化及影响因素分析

分析上海中心城区河流水系1860~2003年变化情况,研究表明:①140余年来中心城区有历史记载河流消失至少310条段,总长超520 km,水面积减少约10.46 km²,水面率下降3.61%。中心城区所在水利片河流水系结构破坏,水系分枝比受河道消亡影响明显降低,水系分维数异常;②中心城区河流水系阶段性集中消亡,消亡驱动力因消亡时期而异;③初步估算,140余年来中心城区河道槽蓄容量减少超过2 029×10⁴m3、单位面积可调蓄容量减少5.06×10⁴m³/km²。河道槽蓄容量较百年前减少超过80%,城市河网调蓄能力明显削弱,且1950~1990年市政雨水泵站累积增加数目与同期区域内河道累积消失数量、长度和槽蓄容量间均呈显著正相关性。     

乌鲁木齐霾天气PM10单颗粒物理化学特征

为了解乌鲁木齐冬季污染天气形成过程中大气颗粒物化学组分的变化,探讨霾天气快速形成的主要原因,应用扫描电镜/X射线能谱仪(SEM/EDX)对2013年2月乌鲁木齐东南郊一次典型霾天和正常天气所采集的大气颗粒物(PM₁₀)样品进行了微观形貌和化学组成的对比分析。结果表明:伴随着霾形成过程细粒子浓度呈现急剧上升趋势,PM_1.0/PM₁₀比值高达0.77。SEM/EDX观测显示,霾天形成前后,PM₁₀的形态结构和元素组成存在较明显的差异,霾天规则的石膏颗粒占规则颗粒的71.1%,明显高于正常天气,表明大气颗粒物的硫化现象比较普遍,大量硅铝酸盐飞灰说明采样点周边有较明显的燃煤源输送;二次无机颗粒组成主要为硫酸盐颗粒,由正常天气的16.1%增加到24.6%,高浓度硫酸盐与乌鲁木齐东南郊此次霾形成有密切的联系。     

Estimating open boundary conditions from coastal tidal observations by adjoint approach

A numerical study for estimating the tidal open boundary conditions of a shelf current modrl from tb coastal tidal observations is presented. The method is based on the optimal control/adjoint method. A lrast square fitting of the model state to simulated data is used. Two ideal domains and coastlines are considered. Using the IAP shallow. water model and its adjoint model, some identical twin experiments are carried out to test efficiency and lirnilsd of the method. The results show that the adjoint method can efficiently estimate the open boundary conditions well for gulf/bay like domains. The adjoint method seems to have great potential to improve the accuracy of tide and shelf current modeling in coastal regions. Project supported hy the National Natural Science Fuundation of China (Grant No. 49376256)     

Influence of sequential tropical cyclones on phytoplankton blooms in the northwestern South China Sea

Upper ocean responses to the passage of sequential tropical cyclones over the northwestern South China Sea(SCS) in 2011 were investigated using satellite remote sensing data,Argo reanalysis data,and an array of mooring data.We found that the sea surface low temperature region lasted for more than 38 days and two phytoplankton blooms occurred after the passage of sequential tropical cyclones.The upper ocean cooling reached 2-5℃ with a right-side bias was observed along the typhoon track to about 200 km.The maintenance of low temperature region and the two phytoplankton blooms were mainly driven by upwelling and near-inertial turbulence mixing induced by the sequential tropical cyclones.The first phytoplankton bloom appeared on the 7~(th) day after the passage of the three tropical cyclones,and the chlorophyll-a(chl-a) concentration increased by 226%,which may be mainly driven by typhoons induced upwelling.The second phytoplankton bloom occurred on the 30~(th) day,the chl-a concentration increased by290%.Further analysis suggested that only the typhoons with similar characteristics as Nesat and Nalgae can induce strong near-inertial oscillation(NIO).Strong turbulent mixing associated with the near-inertial baroclinic shear instability lasted for 26 days.The measured mean eddy diffusivity in the upper ocean was above 10~(-4) m~2/s after typhoon Nesat.Enhancement of the turbulent mixing in the upper ocean helped to transport nutrient-rich cold waters from the deep layer to the euphotic layer,and is a maj or mechanism for the long-term maintenance of low temperature region as well as the second phytoplankton bloom.     

Effect of farmland expansion on drought over the past century in Songnen Plain,Northeast China

The effects of human activities on climate change are a significant area of research in the field of global environmental change. Land use and land cover change(LUCC) has a greater effect on climate than greenhouse gases, and the effect of farmland expansion on regional drought is particularly important. From the 1910 s to the 2010 s, cultivated land in Songnen Plain increased by 2.67 times, the area of cultivated land increased from 4.92×10~4 km~2 to 13.14×10~4 km~2, and its percentage of all land increased from 25% to 70%. This provides an opportunity to study the effects of the conversion of natural grassland to farmland on climate. In this study, the drought indices in Songnen Plain were evaluated from the 1910 s to the 2010 s, and the effect of farmland expansion on drought was investigated using statistical methods and the Weather Research and Forecasting Model based on UK's Climatic Research Unit data. The resulting dryness index, Palmer drought severity index, and standardized precipitation index values indicated a significant drying trend in the study area from 1981 to 2010. This trend can be attributed to increases in maximum temperature and diurnal temperature range, which increased the degree of drought. Based on statistical analysis and simulation, the maximum temperature, diurnal temperature range, and sensible heat flux increased during the growing season in Songnen Plain over the past 100 years, while the minimum temperature and latent heat flux decreased. The findings indicate that farmland expansion caused a drying trend in Songnen Plain during the study period.     

Predictable climate dynamics of abnormal East Asian winter monsoon: once-in-a-century snowstorms in 2007/2008 winter

In 2008 (January–February), East Asia (EA) experiences the most severe and long-persisting snowstorm in the past 100 years. Results in this study show that 2007/2008 winter is dominant by the third principal mode of the East Asian winter monsoon (EAWM) which explains 8.7% of the total surface air temperature variance over EA. Significantly distinguished from the first two leading modes, the third mode positive phase features an increased surface pressure over the northwestern EA, an enhanced central Siberian high (CSH), a strengthened and northwestward extended western Pacific subtropical high (WPSH) and anomalously strong moisture transport from western Pacific, Arabian Sea and Bay of Bengal to EA. It also exhibits an intimate linkage with the sea surface temperature anomalies (SSTAs) in the Arctic Ocean areas adjacent to northern Eurasian continent, central North Pacific and northeastern Pacific. Such SSTAs emerge in prior autumn and persist through ensuing winter, signifying precursory conditions for the anomalous third EAWM mode. Numerical experiments with a simple general circulation model demonstrate that the Arctic SSTAs excite geo-potential height anomalies over northern Eurasian continent and impacts on the CSH, while the extra-tropical Pacific SSTAs deform the WPSH. Co-effects of them play crucial roles on origins of the third EAWM mode. Based on these results, an empirical model is established to predict the third mode of the EAWM. Hindcast is performed for the 1957–2008 period, which shows a quite realistic prediction skill in general and good prediction ability in the extreme phase of the third mode of the EAWM such as 2007/2008 winter. Since all these predictors can be readily monitored in real time, this empirical model provides a real time forecast tool and may facilitate the seasonal prediction of high-impact weather associated with the abnormal EAWM.