用16S rRNA基因的内切酶图谱快速鉴别几种对虾病原菌

坎普氏弧菌是青岛海洋大学生物系微生物实验室于１９８９－１９９０年自对虾养殖场中国对虾红腿病心脏及血淋巴中分离并鉴定的菌株，副溶血菌和溶藻胶弧菌两菌株于１９９４年９月得自中国科学院微生物研究所，为建立快速、准确的中国对虾病原菌的诊断技术，根据几种细菌的１６ＳｒＲＮＡ基因的序列，设计并合成该基因的多聚酶反应的引物ＰＬ１和ＰＬ２。并用该对引物分别从坎普氏弧菌、副溶血弧菌和溶藻胶弧菌的ＤＮＡ要品中扩增出分     

Further evidence for non-reemergence of winter SST anomalies in the North Pacific eastern subtropical mode water area

Following our previous study (Sugimoto and Hanawa, 2005b), we further investigate the reason why reemergence of winter sea surface temperature anomalies does not occur in the North Pacific eastern subtropical mode water (NPESTMW) area, despite its occurrence in the North Pacific subtropical mode water and North Pacific central mode water areas. We use vertical temperature and salinity profiles of the World Ocean Circulation Experiment Hydrographic Program and Argo floats with high vertical and temporal resolution, together with heat flux data through the sea surface. We point out first that one of the causes for non-occurrence of reemergence is that the thickness of NPESTMW is very thin. In addition to this basic cause, two major reasons are found: a vigorous mixing in the lower portion of NPESTMW and less heat input from the atmosphere in the warming season. Since, in the lower portion of NPESTMW and deeper, the stratification is favorable for salt-finger type convection to occur compared with the other mode water areas, vigorous mixing takes place. This is confirmed by both a large Turner Angle there and the existence of staircase structures in vertical temperature and salinity profiles. From the viewpoint of heat input, the NPESTMW area gradually gains heat in the warming season compared with other mode water areas. As a result, NPESTMW cannot be capped so quickly by the shallow summer mixed layer, and water properties of NPESTMW are to be gradually modified, even in the upper portion.     

Geomorphological evidence for upslope canyon-forming processes on the northern KwaZulu-Natal shelf,SW Indian Ocean,South Africa

A geomorphological and statistical analysis of slope canyons from the northern KwaZulu-Natal continental margin is documented and compared with submarine canyons from the Atlantic margin of the USA. The northern KwaZulu-Natal margin is characterized by increasing upslope relief, concave slope-gradient profiles and features related to upslope growth of the canyon forms. Discounting slope-gradient profile, this morphology is strikingly similar to canyon systems of the New Jersey slope. Several phases of canyon incision indicate that downslope erosion is also an important factor in the evolution of the northern KwaZulu-Natal canyon systems. Despite the strong similarities between the northern KwaZulu-Natal and New Jersey slope-canyon systems, key differences are evident: (1) the concavity of the northern KwaZulu-Natal slope, contrasting with the ∼linear New Jersey slope; (2) the relative isolation of the northern KwaZulu-Natal canyons, rather than the dense clustering of the New Jersey canyons; and (3) the absence of strongly shelf-breaching canyons along the northern KwaZulu-Natal margin. In comparison with the New Jersey margin, we surmise a more youthful stage of canyon evolution, a result of either the canyons themselves being younger or the formative processes being less active. Less complicated patterns of erosion resulting from reduced sediment availability have developed in northern KwaZulu-Natal. The reduction in slope concavity on the New Jersey margin may be the result of grading of the upper slope by intensive headward erosion, a process more subdued—or less evident—on the KwaZulu-Natal margin.     

I(L)值完全诱导空间

作者引入 I(L)值完全下半连续映射 ,研究其性质。利用 I(L)值完全下半连续映射定义I(L)值完全诱导空间 ,给出 I(L)值完全诱导空间的拓扑基的表达形式 ,证得两个 I(L)值完全诱导空间的映射是连续映射的充分必要条件 ,并建立了乘积空间的 I(L)值完全诱导空间与 I(L)值完全诱导空间的乘积空间的联系     

The impact of wave loads and pore-water pressure generation on initiation of sediment transport

The build-up of pore-water pressure by waves can lead to sediment liquefaction and subsequent transport by traction currents. This process was investigated by measuring pore-water pressures both in a field experiment and laboratory wave tank tests. Liquefaction was observed in the wave tank tests. The results suggest that sand is less susceptible than silts to wave-induced liquefaction because of the tendency to partially dissipate pore-water pressures. However, previous studies have determined that pore-water pressures must approach liquefaction before current velocities necessary to initiate transport are reduced. Once liquefaction has occurred more sediment can be transported.     

南海晚第四纪硅藻遗体及其地质意义Ⅵ.硅藻的2个新种和7个新记录

本文记述了南海晚第四纪沉积物中发现的两个新种[线形辐环藻（Actinocyclus lineatus Liu,Cheng et Lan sp.nov.）、星圆筛藻（Coscinodiscus aster-aeus Cheng,Liu et Lan sp.nov.）]以及在我国首次记录的圆筛藻的7个种。     

四川椎实螺属一新种(肺螺亚纲:基眼目,椎实螺科)

作者1987年于四川省乐山市大渡河尾段发现椎实螺属一新种,现命名为乐山椎实螺(Lymnaea leshanensis sp.nov.)。该种与日本琵琶湖产的 Lymnaea onychia 相似,但贝壳的形态、齿式、齿形、两性生殖系统的诸器官、外套膜的色带、个体大小、生活环境和动物区系均有所不同。前者属东洋区系,后者属古北区系。     

Types and characters of sub-bottom acoustic reflection in eastern part of Bohai Sea

Ｈｉｇｈ－ｒｅｓｏｌｕｔｉｏｎｓｕｂ－ｂｏｔｔｏｍｐｒｏｆｉｌｅｒｈａｓｒｅｖｅｄｅｄｓｅｄｉｍｅｎｔａｌｓｔｒａｔａａｎｄｅｎｔｉｒｏｎｍｅｎｔｓｉｎｔｈｅｅｕｔｔｍｐ．ｏｆｔｈｅｌ３ｏｈａｉＳｅａｓｉｎｃｅｔｈｅｌｔａｔｅＰｌｅｉｓｔｏｃｅｎｅ．Ａｔｃｃｏｒｄｉｎｇｔｏｔｈｅｃｈａｒａｃｔｅｒｓｏｆａｃａｕｓｅｉｃｒｅｆｌｅｃｔｉｏｎａｎｄｓｅｉｓｍｉｃｔｓｔｒａｔｔｌｇｍｐｈｉｃｐｒｉｎｃｉ－ｐｌｅ，ｓｏｍｅｃｌｔ．ｉｆｉｃａｔｉｏｎｏｆｔｙｐｉｃａｌａｃｏｕｓｔｉｃｒｅｆｌｅｃｔｉｏｎｉｓｎｚａｄｅｏｕｔｔｎｄｗｉＵｂｅｆａｖｏｕｒａｂｌｅｆｏｒｉｎｔｅｒｐｒｅｔａｔｉｏｎａｎｄｒｅｃｏｇｎｂａｔｉｏｎｏｆａｏｏｕｓｔｉｃｐｒｏｆｉｌｅｓｉｎｔｈｉｓａｒｅａ     

一种带自由面的三维船体贴体网络的数值生成

本文提出了一个生成自由面的三维船体贴体网格的数值方法。文中以静水面以上的船体型线作为描述非定常自由面的计算网格域，进一步完善了我们以往开发的无法考虑兴波与静水面以上的船体型线相互作用对船舶粘性流动影响的网格生成方法。静水面以上的初始自由面网格分别沿船面法线η及切线ζ方向的曲线长的指数进行分布，即可极方便地调节自由面在船面及静水面附近的网格间距，也保证了在数值离散三维Ｐｏｉｓｓｏｎ方程时具有相当好的收敛性。这种方法的特点是计算量相当小，尤其能极方便地控制网格在船面簿粘性层中及静水面附近的分布。以Ｓｅｒｉｅｓ６０船模作为计算算例，带自由面的网格分布是相当满意的     

Responses of phytoplankton and heterotrophic bacteria in the northwest subarctic Pacific to in situ iron fertilization as estimated by HPLC pigment analysis and flow cytometry

To verify the hypothesis that the growth of phytoplankton in the Western Subarctic Gyre (WSG), which is located in the northwest subarctic Pacific, is suppressed by low iron (Fe) availability, an in situ Fe fertilization experiment was carried out in the summer of 2001. Changes over time in the abundance and community structure of phytoplankton were examined inside and outside an Fe patch using phytoplankton pigment markers analyzed by high-performance liquid chromatography (HPLC) and flow cytometry (FCM). In addition, the abundance of heterotrophic bacteria was also investigated by FCM. The chlorophyll a concentration was initially ca. 0.9 μg l⁻¹ in the surface mixed layer where diatoms and chlorophyll b-containing green algae (prasinophytes and chlorophytes) were predominant in the chlorophyll biomass. After the iron enrichment, the chlorophyll a concentration increased up to 9.1 μg l⁻¹ in the upper 10 m inside the Fe patch on Day 13. At the same time, the concentration of fucoxanthin (a diatom marker) increased 45-fold in the Fe patch, and diatoms accounted for a maximum 69% of the chlorophyll biomass. This result was consistent with a microscopic observation showing that the diatom Chaetoceros debilis had bloomed inside the Fe patch. However, chlorophyllide a concentrations also increased in the Fe patch with time, and reached a maximum of 2.2 μg l⁻¹ at 5 m depth on Day 13, suggesting that a marked abundance of senescent algal cells existed at the end of the experiment. The concentration of peridinin (a dinoflagellate marker) also reached a maximum 24-fold, and dinoflagellates had contributed significantly (>15%) to the chlorophyll biomass inside the Fe patch by the end of the experiment. Concentrations of 19′-hexanoyloxyfucoxanthin (a prymnesiophyte marker), 19′-butanoyloxyfucoxanthin (a pelagophyte marker), and alloxanthin (a cryptophyte marker) were only incremented a few-fold increment inside the Fe patch. On the contrary, chlorophyll b concentration reduced to almost half of the initial level in the upper 10 m water column inside the Fe patch at the end of the experiment. A decrease with time in the abundance of eukaryotic ultraphytoplankton (<ca. 5 μm in size), in which chlorophyll b-containing green algae were possibly included was also observed by FCM. Overall, our results indicate that Fe supply can dramatically alter the abundance and community structure of phytoplankton in the WSG. On the other hand, cell density of heterotrophic bacteria inside the Fe patch was maximum at only ca. 1.5-fold higher than that outside the Fe patch. This indicates that heterotrophic bacteria abundance was little respondent to the Fe enrichment.