基于形态学和DNA条形码探讨“南鲳”物种有效性

众所周知鲳科鱼类均为重要的经济种类,在中国沿海仅见鲳属(Pampus)鱼类分布,而低鳍鲳属(Peprilus)和真鲳属(Stromateus)则分布于大西洋和东太平洋。本研究采用形态学和DNA条形码技术对海南、广西和广东水产品市场上出现的“南鲳”进行物种鉴定,同时下载该物种的COⅠ基因同源序列进行比较分析,并纠正GenBank中的错误序列。研究结果显示“南鲳”学名实为中间低鳍鲳(Peprilus medius),为冰冻进口货品,在我国沿海无该物种的分布报道,其与国内已知的6种鲳属鱼类在鳃耙、背鳍、臀鳍等外部形态上存在明显的差异,在COⅠ基因水平上与鲳属鱼类遗传距离为0.138,分化于中新世晚期。本研究可为水产品市场种类鉴定提供借鉴,也为鲳科鱼类的研究和海洋鱼类分类学提供基础数据和参考。     

长江口新桥水道动力地貌变化过程研究

以泰国湾 T155号柱状沉积物为研究对象,利用地球化学和矿物学手段探讨了全新世以来泰国湾古气候演化历史。(La/Sm)UCC-(Gd/Yb)UCC指示了全新世以来泰国湾西南部物质来源较为稳定,主要来自于马来半岛,季风控制下的沿岸流是其主要驱动力。通过对高岭石/(绿泥石+伊利石)(ωk/(ωc+ωl))、化学蚀变指数(CIA)和钾铝质量分数比值(ωK/ωAl)的变化规律进行综合研究,将全新世以来泰国湾古气候演化过程划分为两个阶段:①早中全新世(12000~6500calaBP):ωk/(ωc+ωl)值和CIA 值较高,ωK/ωAl值则较低,指示了该段时期强盛的东亚季风;发生于11200~10500calaBP和8500~7500calaBP的冷事件指示了泰国湾对新仙女木事件和8.2ka冷事件的响应;②中全新世(6500calaBP)以来:ωk/(ωc+ωl)值和 CIA 值明显下降,ωK/ωAl值则逐渐上升,指示了该段时间东亚季风呈逐渐减弱的趋势;在3200~1800calaBP时地球化学和黏土矿物指标呈现一个明显低值,对应于热带海域“斜氏普林虫低值事件”,揭示了全球气候变化在泰国湾海域良好的区域性响应特征。     

两种混养方式对异枝江蓠(Gracilaria bailinae)生长性能、表面附生细菌群落及抗生素抗性基因的影响

为揭示"鱼-藻"和"鱼-虾-藻"混养对异枝江蓠(Gracilaria bailinae)生长性能、表面附生细菌群落和抗生素抗性基因(antibiotic resistance genes,ARGs)的影响,阐明异枝江蓠表面附生细菌群落与生长性能、ARGs之间的关系,利用16S rDNA高通量测序技术和Real-time qPCR技术分析了异枝江蓠表面附生细菌群落和ARGs的组成与差异,冗余分析(RDA)探讨细菌群落与生长性能、ARGs之间的关联。结果表明:(1)"鱼-虾-藻"混养会促进异枝江蓠的生长性能,增加表面附生细菌群落的多样性。(2)异枝江蓠表面附生细菌群落主要属于变形菌门、蓝藻门、浮霉菌门和拟杆菌门,不同混养方式中优势菌属组成不同,"鱼-虾-藻"混养优势菌属多样性较高。(3)"鱼-虾-藻"混养的异枝江蓠ARGs/MGEs的相对丰度大多高于"鱼-藻"混养。(4)RDA分析表明,生长性能主要与Ralstonia、Blastopirellula等显著相关,ARGs/MGEs主要与Nitrosomonas、Alteromonas、Pleurocapsa;CC-7319等显著相关。"鱼-虾-藻"混养能够增强异枝江蓠的生长性能,提高异枝江蓠表面附生细菌群落的多样性。但"鱼-虾-藻"混养能够增加异枝江蓠ARGs/MGEs的相对丰度,存在一定的生态风险。因此,在注重经济效益的同时也要关注可能存在的对人类健康的危害。研究结果将有助于海水养殖环境的优化,为大型海藻在海水养殖业中的应用与推广提供理论基础。     

南极菲尔德斯半岛潮间带沉积物细菌的多样性及分离菌产酶测定

摘要:南极菲尔德斯半岛具有多种不同特征的生态地理微环境,如长城科考站、企鹅岛、生物湾与黄金湾流域、半岛南部、碧玉滩等。这些区域因水文地质、动植物分布、人类活动程度等不同而具有明显的生态地理差异。本研究从这些微环境海岸潮间带采集了7份代表性沉积物样品,采用16S rRNA基因高通量测序方法,对其细菌类群的多样性以及环境理化因子的影响进行了比较分析;同时采用常规可培养鉴定方法,对样品的分离菌株产酶状况进行了初步测定。结果表明：整体上看,所有样品的细菌类群主要分布在45门、104纲、442属当中,表现出比较高的多样性。其中,优势菌门Proteobacteria主要分布在半岛南部、碧玉滩和生物湾潮间带沉积物物中,优势菌门Bacteroidetes主要分布在企鹅岛两侧和黄金湾潮间带沉积物,长城站潮间带沉积物的优势门为Firmicutes。相似生态微环境的潮间带沉积物具有不同的优势菌门和纲;而不同生态微环境潮间带沉积物却具相同的优势菌门和纲。有机氮（TON）、NH4+-N、NO3--N、PO43--P、NO2--N含量与企鹅岛两侧、生物湾、黄金湾潮间带沉积物的菌群多样性具有相关性,其中TON对生物湾样品的影响最大,而有机碳（TOC）对所有样品的影响较小。分离菌株的产酶实验表明：在企鹅岛、生物湾、黄金湾等动物频繁出没的潮间带样品中,蕴藏着一批产淀粉酶、酪蛋白酶、脂肪酶、七叶苷酶、过氧化氢酶能力较强菌株,这为今后进一步的应用开发提供了宝贵的低温产酶菌株来源。 关键词: 菲尔德斯半岛; 潮间带沉积物; 细菌类群多样性; 产酶菌株     

杭州湾北岸临港新片区岸段潮滩沉积特征

近年来,海洋生态系统生态修复成效评估研究日益受到关注。本文梳理了国内外在红树林、珊瑚礁、海草床、盐沼湿地等四类典型海洋生态系统生态修复成效评估指标和评估方法方面的研究进展。结果表明,典型海洋生态系统在进行生态修复成效评估时,基于修复目标和修复方法,评估指标选取虽各有侧重,但主要包括生物和生境状况两个方面。多数生态修复项目的监测、评估都是在短期内进行的,缺乏长期、连续性监测,社会和经济相关指标的研究还相对较为缺乏。成效评估方法目前没有比较清晰、全面的分类。实际应用的评估方法较为单一,对海洋生态系统服务价值揭示不够全面。     

Observations and modelling of the travel time delay and leading negative phase of the 16 September 2015 Illapel,Chile tsunami

The systematic discrepancies in both tsunami arrival time and leading negative phase (LNP) were identified for the recent transoceanic tsunami on 16 September 2015 in Illapel, Chile by examining the wave characteristics from the tsunami records at 21 Deep-ocean Assessment and Reporting of Tsunami (DART) sites and 29 coastal tide gauge stations. The results revealed systematic travel time delay of as much as 22 min (approximately 1.7％ of the total travel time) relative to the simulated long waves from the 2015 Chilean tsunami. The delay discrepancy was found to increase with travel time. It was difficult to identify the LNP from the near-shore observation system due to the strong background noise, but the initial negative phase feature became more obvious as the tsunami propagated away from the source area in the deep ocean. We determined that the LNP for the Chilean tsunami had an average duration of 33 min, which was close to the dominant period of the tsunami source. Most of the amplitude ratios to the first elevation phase were approximately 40％, with the largest equivalent to the first positive phase amplitude. We performed numerical analyses by applying the corrected long wave model, which accounted for the effects of seawater density stratification due to compressibility, self-attraction and loading (SAL) of the earth, and wave dispersion compared with observed tsunami waveforms. We attempted to accurately calculate the arrival time and LNP, and to understand how much of a role the physical mechanism played in the discrepancies for the moderate transoceanic tsunami event. The mainly focus of the study is to quantitatively evaluate the contribution of each secondary physical effect to the systematic discrepancies using the corrected shallow water model. Taking all of these effects into consideration, our results demonstrated good agreement between the observed and simulated waveforms. We can conclude that the corrected shallow water model can reduce the tsunami propagation speed and reproduce the LNP, which is observed for tsunamis that have propagated over long distances frequently. The travel time delay between the observed and corrected simulated waveforms is reduced to <8 min and the amplitude discrepancy between them was also markedly diminished. The incorporated effects amounted to approximately 78％ of the travel time delay correction, with seawater density stratification, SAL, and Boussinesq dispersion contributing approximately 39％, 21％, and 18％, respectively. The simulated results showed that the elastic loading and Boussinesq dispersion not only affected travel time but also changed the simulated waveforms for this event. In contrast, the seawater stratification only reduced the tsunami speed, whereas the earth's elasticity loading was responsible for LNP due to the depression of the seafloor surrounding additional tsunami loading at far-field stations. This study revealed that the traditional shallow water model has inherent defects in estimating tsunami arrival, and the leading negative phase of a tsunami is a typical recognizable feature of a moderately strong transoceanic tsunami. These results also support previous theory and can help to explain the observed discrepancies.     

Geological characteristics of the Qiaoyue Seamount and associated ultramafic-hosted seafloor hydrothermal system (～52.1°E,Southwest Indian Ridge)

Hydrothermal vent incidence was once thought to be proportional to the spreading rate of the mid-ocean ridges (MORs). However, more and more studies have shown that the ultraslow-spreading ridges (e.g., Southwest Indian Ridge (SWIR)) have a relatively higher incidence of hydrothermal venting fields. The Qiaoyue Seamount (52.1°E) is located at the southern side of segment #25 of the SWIR, to the west of the Gallieni transform fault. The Chinese Dayang cruises conducted eight preliminary deep-towed surveys of hydrothermal activity in the area during 2009 and 2018. Here, through comprehensive analyses of the video and photos obtained by the deep-towed platforms, rock samples, and water column turbidity anomalies, a high-temperature, ultramafic-hosted hydrothermal system is predicted on the northern flank of the Qiaoyue Seamount. We propose that this hydrothermal system is most likely to be driven by gabboric intrusions. Efficient hydrothermal circulation channels appear against a backdrop of high rock permeability related to the detachment fault.     

锦州湾表层海水微塑料分布特征

海洋微塑料是全球关注的新兴环境问题,海湾由于特殊的地理环境特征,成为微塑料分布研究的热点区域。本研究以锦州湾为研究海域,于2017年10月布设了11个点位开展表层海水微塑料样品采集,在实验室采用湿式氧化法开展样品前处理,应用傅立叶变换显微红外光谱仪分析鉴定微塑料成分。研究结果表明,锦州湾表层水体微塑料平均丰度为(0.93±0.59)个/m³,微塑料数量占全部塑料样品的96.2%。微塑料的主要成分为聚丙烯和聚乙烯,分别占55.0%和23.5%;线状和片状塑料的比例最高,分别占41.7%和26.2%;白色、蓝色和半透明微塑料分别占35.1%、26.0%和21.4%。受水动力条件和陆域河流输入等影响,锦州湾表层水体中微塑料的空间分布整体呈现北部偏高,向南部递减的趋势。     

Levels and patterns of genetic variation in Pampus minor:Assessment of a mitochondrial DNA control region sequence

Pampus minor is an important commercial fish. Due to the similarity of external morphological characteristics among the genus Pampus species, P. minor has often been identified as the juvenile group of both P. cinereus and P. argenteus. While little genetic background on this species is known, this study was based on control region sequences and provided the first evaluation of the genetic signature of 264 individuals of P. minor from 11 populations along the coasts of China and Malaysia. The results indicate high genetic haplotype diversity and low nucleotide diversity in this species. Additionally, two differentiated haplotype lineages were identified in the P.minor populations. However, phylogenetic structures corresponding to the geographical locations were unable to be established. Analysis of molecular variance identified a vast majority of the genetic variation occurring within populations. F-statistic test value(FST) of pairwise indicated that great differences existed between the Chinese and Malaysian P. minor populations. For the Chinese populations, the genetic differences were insignificant with the exception of the Xiamen population, which is a marginal population. During the late Pleistocene, a population expansion of P. minor occurred. These expanded populations originated from the glacial refugium in the South China Sea and then rapidly occupied and adapted to their new habitat. The results of this study provide genetic information for ensuring the protection and management of P. minor resources.     

海南省海水鱼养殖产业现状调研与对策研究

文章基于“结构-行为-绩效”（SCP）范式,从市场结构、市场行为和市场绩效3个方面分析海南省的海水鱼养殖产业。发现：海水鱼养殖业属于原子型市场结构,市场集中度较低,龙头企业竞争力不足;总体进入壁垒较低,市场进入难度较小;种苗产业链分工和定价随机性强,鱼卵价格出现经常性短期波动;海水养殖业资源配置效率较低,许多企业面临环保督察、经营管理、人力资源等诸多危机,产业技术进步贡献度不足。因此,提出如下建议：海南海水鱼养殖业需要在兼顾生态文明建设的前提下,政府需要制定长远的渔业发展规划;加快推进行业的分工与专业化的生产;通过兼并、强强联合加快推进规模经济,进一步提高产品附加值,提高产业综合竞争力。